SSGCID: Target Page

BatrA.18409.a

TrwN protein (Trw type IV secretion machinery)

CENTER ID:	BatrA.18409.a
ORGANISM:	Bartonella tribocorum CIP 105476 / IBS 506
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BatrA.18409.a.B1.GE35762	full length		1	213

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|382640.5.peg.2547
RefSeq:	YP_001610466.1
UniProt:	A9IZ24

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIFSDFTMFA AAYISNTPHL AFSALIEQGA RDNVDITSNN DKLFGQLSPL REETAITEQL KQNKHNFDAI FEQIDVENMK WFSFFGAFGF CRDLADVQSA LACCYKQKVS KCVFEKAALQ SALSFYNTKG LNSGADTQTI TSYIGVKAAA QEDKNLQDPV RLKKEEPEQV TKVDSLPLSS EELEDAFGHK ESSSRDAFAA EGSSSSTEQL SGN

NT Sequence

atgatttttt cagatttcac aatgtttgct gcggcataca tatccaatac ccctcattta gcattttcag ctctcatcga acaaggcgca cgggataatg ttgatataac aagcaataat gataaattat tcggtcaatt atctccactt agagaagaaa ctgcaataac tgagcagctc aaacaaaata aacataattt tgacgcaatc tttgagcaaa ttgatgttga aaatatgaaa tggttttctt tttttggggc atttggcttt tgcagagatc ttgcagatgt acaaagtgct ttagcttgct gttacaaaca aaaagtatca aagtgtgttt ttgaaaaagc ggcattacaa tctgcgttaa gtttttataa cacaaaaggc ttaaacagtg gtgccgatac acaaacgatt acttcatata ttggagtaaa agctgctgca caagaagata aaaacttgca agatcctgta aggctaaaga aagaagaacc agaacaagta actaaagtag attctcttcc tctttcttca gaggaattag aagatgcgtt tgggcataaa gaaagtagca gccgtgatgc ttttgctgcg gaaggttctt cttcatcgac cgagcagcta agcggtaatt ga

Details for BatrA.18409.a.B1.GE35762

HARVESTED ON:	7/31/2012
SEQUENCED ON:	8/3/2012
EXPECTED MW:	24kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI FSDFTMFAAA YISNTPHLAF SALIEQGARD NVDITSNNDK LFGQLSPLRE ETAITEQLKQ NKHNFDAIFE QIDVENMKWF SFFGAFGFCR DLADVQSALA CCYKQKVSKC VFEKAALQSA LSFYNTKGLN SGADTQTITS YIGVKAAAQE DKNLQDPVRL KKEEPEQVTK VDSLPLSSEE LEDAFGHKES SSRDAFAAEG SSSSTEQLSG N

Validated NT Sequence

aattttgttt nnctttaaga aggagatata ccatggctca ccaccaccac caccatatga ttttttcaga tttcacaatg tttgctgcgg catacatatc caatacccct catttagcat tttcagctct catcgaacaa ggcgcacggg ataatgttga tataacaagc aataatgata aattattcgg tcaattatct ccacttagag aagaaactgc aataactgag cagctcaaac aaaataaaca taattttgac gcaatctttg agcaaattga tgttgaaaat atgaaatggt tttctttttt tggggcattt ggcttttgca gagatcttgc agatgtacaa agtgctttag cttgctgtta caaacaaaaa gtatcaaagt gtgtttttga aaaagcggca ttacaatctg cgttaagttt ttataacaca aaaggcttaa acagtggtgc cgatacacaa acgattactt catatattgg agtaaaagct gctgcacaag aagataaaaa cttgcaagat cctgtaaggc taaagaaaga agaaccagaa caagtaacta aagtagattc tcttcctctt tcttcagagg aattagaaga tgcgtttggg cataaagaaa gtagcagccg tgatgctttt gctgcggaag gttcttcttc atcgaccgag cagctaagcg gtaattaagt gagtaagata ggatccggct gctaacaagc ccnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattt tttcagattt cacaatgttt gctgcggcat acatatccaa tacccctcat ttagcatttt cagctctcat cgaacaaggc gcacgggata atgttgatat aacaagcaat aatgataaat tattcggtca attatctcca cttagagaag aaactgcaat aactgagcag ctcaaacaaa ataaacataa ttttgacgca atctttgagc aaattgatgt tgaaaatatg aaatggtttt ctttttttgg ggcatttggc ttttgcagag atcttgcaga tgtacaaagt gctttagctt gctgttacaa acaaaaagta tcaaagtgtg tttttgaaaa agcggcatta caatctgcgt taagttttta taacacaaaa ggcttaaaca gtggtgccga tacacaaacg attacttcat atattggagt aaaagctgct gcacaagaag ataaaaactt gcaagatcct gtaaggctaa agaaagaaga accagaacaa gtaactaaag tagattctct tcctctttct tcagaggaat tagaagatgc gtttgggcat aaagaaagta gcagccgtga tgcttttgct gcggaaggtt cttcttcatc gaccgagcag ctaagcggta attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat