BatrA.18407.a

TrwE protein (Trw type IV secretion machinery)

CENTER ID: BatrA.18407.a
ORGANISM: Bartonella tribocorum CIP 105476 / IBS 506
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BatrA.18407.a.B2.GE35760 soluble domain 63 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|382640.5.peg.2574
RefSeq: YP_001610493.1
UniProt: A9IZ73

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFDNKEGDEK GKKLDNIEQK HIENAYESSE LGSEHRPTIP GTRVLLIVGL IVIVAVPIAL TWKAFKMRNT VEIEEEKPQQ TVQQIIPSYT PRVIEEPKLV EESEKSTPIE DSSLDISPAL AKLLQTTVPP HMIQDSEELA RRRMLSSSLN NGGSEGSTET NKASNDNSGA LNLQPVRLGQ SHAAQLRNRD LLITQGTQID CTLETKIITS QPGMTTCHLT RDIYSTSGRV VLLDRGSKVV GFYQSGLQQG QTRVFVQWLR IETPSGVIVN LDSPGTGPLG EAGIGGWVDR HFWERFSGAI MVSIIGDLGE WVRGKINKSS KENKENAQPQ GAHNAELIVN DVIQNSINIT PTLYKHQGER VNIFVARDLD FSDVYSLVTR
NT Sequence
atgtttgaca ataaggaagg agatgaaaaa ggcaaaaaac tggacaatat agagcaaaaa catattgaaa atgcctatga aagctccgaa ctaggttcag agcaccgtcc gacaattcca ggtacccgtg tattattaat cgtaggactt attgtcatcg tagcggtacc aattgctttg acttggaaag cttttaaaat gcgtaatacc gtagaaattg aggaagaaaa accgcaacaa acggtacagc aaattatacc aagctatacc ccgcgggtta tagaagagcc aaagcttgta gaagaatcag aaaaatcaac accaatagaa gactcaagcc tagatatttc accggccttg gctaagcttc ttcaaacaac cgttccccca catatgattc aagattctga agaattagcg cgcagacgta tgctcagttc tagtcttaac aatggtggaa gtgaaggatc tacggagaca aataaagcat cgaatgacaa cagtggtgca ttaaatctcc aacctgtacg cttaggtcaa tcacatgcgg cgcaacttcg taaccgtgat ctcttaatta cgcaaggaac gcaaatagat tgtacgttgg aaacaaaaat tattacctca caaccaggga tgacaacatg tcatttaacg cgggatattt attctacaag tggtcgtgtt gttttgctcg atcgtggttc taaagtcgtt ggtttttacc aaagtggctt acagcaaggg caaacacgtg tttttgtgca atggttacgt attgaaacgc cttctggtgt tattgtcaat cttgactcac ctgggaccgg tcctcttggt gaagcaggta tcggtggctg ggttgataga cacttttggg agcggtttag tggcgcaatt atggtgagta tcatcggtga tttaggagaa tgggtgagag gtaaaattaa taaaagtagc aaagagaata aagagaacgc acagccacaa ggagctcata atgctgaact tatagtaaac gatgttattc aaaattcaat caatataaca ccaacacttt acaaacacca aggcgaacga gtaaatattt ttgttgctcg tgatctggat ttcagcgatg tctacagtct cgtcacacgt taa
Details for BatrA.18407.a.B2.GE35760
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/6/2012
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMK AFKMRNTVEI EEEKPQQTVQ QIIPSYTPRV IEEPKLVEES EKSTPIEDSS LDISPALAKL LQTTVPPHMI QDSEELARRR MLSSSLNNGG SEGSTETNKA SNDNSGALNL QPVRLGQSHA AQLRNRDLLI TQGTQIDCTL ETKIITSQPG MTTCHLTRDI YSTSGRVVLL DRGSKVVGFY QSGLQQGQTR VFVQWLRIET PSGVIVNLDS PGTGPLGEXG IGGWVDRHFW ERFSGAIMVS IIGXLXEWVR GKINKSSXRI KRTHSHXSS
Validated NT Sequence
ataccatggc tcaccaccac caccaccata tgaaagcttt taaaatgcgt aataccgtag aaattgagga agaaaaaccg caacaaacgg tacagcaaat tataccaagc tataccccgc gggttataga agagccaaag cttgtagaag aatcagaaaa atcaacacca atagaagact caagcctaga tatttcaccg gccttggcta agcttcttca aacaaccgtt cccccacata tgattcaaga ttctgaagaa ttagcgcgca gacgtatgct cagttctagt cttaacaatg gtggaagtga aggatctacg gagacaaata aagcatcgaa tgacaacagt ggtgcattaa atctccaacc tgtacgctta ggtcaatcac atgcggcgca acttcgtaac cgtgatctct taattacgca aggaacgcaa atagattgta cgttggaaac aaaaattatt acctcacaac cagggatgac aacatgtcat ttaacgcggg atatttattc tacaagtggt cgtgttgttt tgctcgatcg tggttctaaa gtcgttggtt tttaccaaag tggcttacag caagggcaaa cacgtgtttt tgtgcaatgg ttacgtattg aaacgccttc tggtgttatt gtcaatcttg actcacctgg gaccggtcct cttggtgaan caggtatcgg tggctgggtt gatagacact tttgggagcg gtttagtggc gcaattatgg tgagtatcat cggtgantta ngagaatggg tgagaggnaa aattaataaa agtagcnnga gaataaagag aacgcacagc cacannagct cataatgctg aacttatagt aaacgatgtt attc
Expected Protein Sequence
MAHHHHHHMK AFKMRNTVEI EEEKPQQTVQ QIIPSYTPRV IEEPKLVEES EKSTPIEDSS LDISPALAKL LQTTVPPHMI QDSEELARRR MLSSSLNNGG SEGSTETNKA SNDNSGALNL QPVRLGQSHA AQLRNRDLLI TQGTQIDCTL ETKIITSQPG MTTCHLTRDI YSTSGRVVLL DRGSKVVGFY QSGLQQGQTR VFVQWLRIET PSGVIVNLDS PGTGPLGEAG IGGWVDRHFW ERFSGAIMVS IIGDLGEWVR GKINKSSKEN KENAQPQGAH NAELIVNDVI QNSINITPTL YKHQGERVNI FVARDLDFSD VYSLVTR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag cttttaaaat gcgtaatacc gtagaaattg aggaagaaaa accgcaacaa acggtacagc aaattatacc aagctatacc ccgcgggtta tagaagagcc aaagcttgta gaagaatcag aaaaatcaac accaatagaa gactcaagcc tagatatttc accggccttg gctaagcttc ttcaaacaac cgttccccca catatgattc aagattctga agaattagcg cgcagacgta tgctcagttc tagtcttaac aatggtggaa gtgaaggatc tacggagaca aataaagcat cgaatgacaa cagtggtgca ttaaatctcc aacctgtacg cttaggtcaa tcacatgcgg cgcaacttcg taaccgtgat ctcttaatta cgcaaggaac gcaaatagat tgtacgttgg aaacaaaaat tattacctca caaccaggga tgacaacatg tcatttaacg cgggatattt attctacaag tggtcgtgtt gttttgctcg atcgtggttc taaagtcgtt ggtttttacc aaagtggctt acagcaaggg caaacacgtg tttttgtgca atggttacgt attgaaacgc cttctggtgt tattgtcaat cttgactcac ctgggaccgg tcctcttggt gaagcaggta tcggtggctg ggttgataga cacttttggg agcggtttag tggcgcaatt atggtgagta tcatcggtga tttaggagaa tgggtgagag gtaaaattaa taaaagtagc aaagagaata aagagaacgc acagccacaa ggagctcata atgctgaact tatagtaaac gatgttattc aaaattcaat caatataaca ccaacacttt acaaacacca aggcgaacga gtaaatattt ttgttgctcg tgatctggat ttcagcgatg tctacagtct cgtcacacgt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat