BatrA.18398.a

VirD4 homologue (VirB type IV secretion machinery)

CENTER ID: BatrA.18398.a
ORGANISM: Bartonella tribocorum CIP 105476 / IBS 506
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BatrA.18398.a.B2.GE35757 soluble domain 143 638
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BatrA.18398.a.B2.PS01672 soluble domain 143 638

External Resources

RESOURCE REFERENCE ID
RefSeq: WP_012232127.1
UniProt: Q8GJ55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKYTKTQMVL ILIPIALGTL TIFLVPHFLL LITNGLKSNQ IYWTLRSEPL LILVLTAAIS LLYSLSQKLH LRKTITFVSV VFFGTIALYY VGSEIKRLSP YVGQQGITWS YALQFMDPMV VFGIIIGTFF SLIQFITTSP PKNKVKRAKK EVFGGAAWMD LREAAKIFPA DGQIVVGERY RVDQDNVCKI PFAPGNKTTW GKGGAVPLLT FNLDFGSTHM IFFAGSGGYK TTSTVIPTCL TYPGSIICLD PSTEVAPIVK FARQKMGNRN VIILDPNSLL TKSFNVIDWL LDDSVPRTQR EANIVSFSKL LLTDKKSDNS SAEYFSTQAH NLLTALLAHV IFSDEYEDHE RNLKTLRGIL SQSETAIINQ LRMIQETTPS PFIREMVGIF TEMAEQTFSG VYTTASKDTQ WLSLSNYADL VCGDDFSSSD IANGNIDVFL NLPASILNSY PAIGRVIIGA FLNAMVTADG NYKKRVLFVL DEVDLLGYMN ILEEARDRGR KYGTSLMLFY QSTGQLVNHF GESGARSWFE SCSFVSYAAI KDLQTAKEIS ERCGQMTVEV TGVSKSRGLS LGKSSYNINY QQRALILPHE VIQEMRQDEQ IILMQGQPPL RCGRAIYFRR KEMLAASAKN RFAPQKKN
NT Sequence
atgaaatata ccaagacaca aatggtcctt attttaatcc ctattgcttt aggtacttta acgatattcc ttgtccctca ttttttgttg ttgataacaa atggactaaa gagtaatcaa atttattgga ctcttcgttc agagccttta ttaatcttag tactaacagc tgctatttca ttgttataca gtctctctca aaaactacac ctacgtaaaa caattacttt tgtctccgtt gtcttttttg gaaccattgc tctttactat gttgggagtg aaataaaacg cttaagtccc tatgttggtc aacaaggaat aacgtggagt tatgcgcttc aatttatgga tcctatggtt gtctttggca tcatcattgg tacttttttt tctctcattc aattcattac aacctctcca cctaaaaata aggtcaagcg tgccaaaaaa gaggtttttg ggggagcggc atggatggat ttaagagaag cagcaaaaat ctttcctgcc gatgggcaaa ttgttgtggg ggaaagatac cgtgttgatc aagataatgt atgcaaaatt ccatttgcac ctggcaataa aacaacatgg gggaaaggtg gagcagtacc tttgctaacc tttaatcttg attttggttc aacccatatg atcttttttg ctggttctgg tggttataaa accacaagta cagtaattcc tacgtgttta acctatccag ggtctattat ttgtcttgat ccttcaacag aagttgcccc aatcgtcaaa tttgctcgtc aaaagatggg caatagaaat gttattattc ttgatcctaa ttcactttta acaaaaagtt ttaatgtcat cgattggctt ttagacgaca gtgtaccccg cacacaacgt gaagcaaata ttgttagctt ttccaaattg cttctcacgg ataaaaaatc agacaattct tcggcggaat atttctcgac acaagcccat aatcttttaa cggctcttct tgctcatgtg atattttctg atgaatatga agaccatgag cgcaacttaa aaacattgcg tggaattctc tctcaatcag aaacagctat tatcaatcaa ttacgcatga ttcaagaaac aaccccttct ccttttattc gtgaaatggt gggtattttt acagaaatgg cagaacaaac tttttcagga gtctatacaa ctgcctcaaa agacacccaa tggctttctt tgtctaatta cgcagatctt gtctgcggtg atgatttttc ctcctcagat attgccaatg gtaatataga tgtttttctc aatctccccg ccagcatttt aaacagctat ccagcgatag gacgtgtaat tattggtgcc tttctcaatg ctatggttac agcagacggt aactataaaa agcgtgtttt atttgtctta gatgaagttg atcttctagg ctatatgaat attttagaag aagctcgtga tcgtggacgt aaatacggca catccttaat gctcttttat caatccacgg gtcagttggt caatcacttt ggtgaatcag gggcacgttc atggtttgaa agctgctcct ttgtcagtta tgctgccatt aaagacctcc aaacagcgaa agagatttca gaacgctgtg gtcaaatgac cgttgaagtc accggagtaa gcaaatcaag aggtttatct ttaggaaaga gctcttacaa tatcaattac caacaaagag ctctcatttt accccacgag gttattcaag aaatgcgtca agatgaacaa attattctta tgcaagggca acctcccttg cgatgtggcc gtgccatcta tttcagaaga aaagaaatgt tagctgcttc tgcaaaaaat cgttttgccc cacaaaagaa aaactag
Details for BatrA.18398.a.B2.GE35757
HARVESTED ON: 7/31/2012
SEQUENCED ON: 10/23/2013
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMN KVKRAKKEVF GGAAWMDLRE AAKIFPADGQ IVVGERYRVD QDNVCKIPFA PGNKTTWGKG GAVPLLTFNL DFGSTHMIFF AGSGGYKTTS TVIPTCLTYP GSIICLDPST EVAPIVKFAR QKMGNRNVII LDPNSLLTKS FNVIDWLLDD SVPRTQREAN IVSFSKLLLT DKKSDNSSAE YFSTQAHNLL TALLAHVIFS DEYEDHERNL KTLRGILSQS ETAIINQLRM IQETTPSPFI REMVGIFTEM AEQTFSGVYT TASKDTQWLS LSNYADLVCG DDFSSSDIAN GNIDVFLNLP ASILNSYPAI GRVIIGAFLN AMVTADGNYK KRVLFVLDEV DLXGYXNILE EARDRGRKYG TSLMLFYQST GQLVNHFGES GARSWFESCS FVSYAAIKDL QTAKEISERC GQMTVEVTGV SKSRGLSLGK SSYNINYQQR ALILPHEVIQ EMRQDEQIIL MQGQPPLRCG RAIYFRRKEM LAASA
Validated NT Sequence
atggctcacc accaccacca ccatatgaat aaggtcaagc gtgccaaaaa agaggttttt gggggagcgg catggatgga tttaagagaa gcagcaaaaa tctttcctgc cgatgggcaa attgttgtgg gggaaagata ccgtgttgat caagataatg tatgcaaaat tccatttgca cctggcaata aaacaacatg ggggaaaggt ggagcagtac ctttgctaac ctttaatctt gattttggtt caacccatat gatctttttt gctggttctg gtggttataa aaccacaagt acagtaattc ctacgtgttt aacctatcca gggtctatta tttgtcttga tccttcaaca gaagttgccc caatcgtcaa atttgctcgt caaaagatgg gcaatagaaa tgttattatt cttgatccta attcactttt aacaaaaagt tttaatgtca tcgattggct tttagacgac agtgtacccc gcacacaacg tgaagcaaat attgttagct tttccaaatt gcttctcacg gataaaaaat cagacaattc ttcggcggaa tatttctcga cacaagccca taatctttta acggctcttc ttgctcatgt gatattttct gatgaatatg aagaccatga gcgcaactta aaaacattgc gtggaattct ctctcaatca gaaacagcta ttatcaatca attacgcatg attcaagaaa caaccccttc tccttttatt cgtgaaatgg tgggtatttt tacagaaatg gcagaacaaa ctttttcagg agtctataca actgcctcaa aagacaccca atggctttct ttgtctaatt acgcagatct tgtctgcggt gatgattttt cctcctcaga tattgccaat ggtaatatag atgtttttct caatctcccc gccagcattt taaacagcta tccagcgata ggacgtgtaa ttattggtgc ctttctcaat gctatggtta cagcagacgg taactataaa aagcgtgttt tatttgtctt agatgaagtt gatctnntng gctatnngaa tattttagaa gaagctcgtg atcgtggacg taaatacggc acatccttaa tgctctttta tcaatccacg ggtcagttgg tcaatcactt tggtgaatca ggggcacgtt catggtttga aagctgctcc tttgtcagtt atgctgccat taaagacctc caaacagcga aagagatttc agaacgctgt ggtcaaatga ccgttgaagt caccggagta agcaaatcaa gaggtttatc tttaggaaag agctcttaca atatcaatta ccaacaaaga gctctcattt taccccacga ggttattcaa gaaatgcgtc aagatgaaca aattattctt atgcaagggc aacctccctt gcgatgtggc cgtgccatct atttcagaag aaaagaaatg ttagctgctt ctgcaaa
Expected Protein Sequence
MAHHHHHHMN KVKRAKKEVF GGAAWMDLRE AAKIFPADGQ IVVGERYRVD QDNVCKIPFA PGNKTTWGKG GAVPLLTFNL DFGSTHMIFF AGSGGYKTTS TVIPTCLTYP GSIICLDPST EVAPIVKFAR QKMGNRNVII LDPNSLLTKS FNVIDWLLDD SVPRTQREAN IVSFSKLLLT DKKSDNSSAE YFSTQAHNLL TALLAHVIFS DEYEDHERNL KTLRGILSQS ETAIINQLRM IQETTPSPFI REMVGIFTEM AEQTFSGVYT TASKDTQWLS LSNYADLVCG DDFSSSDIAN GNIDVFLNLP ASILNSYPAI GRVIIGAFLN AMVTADGNYK KRVLFVLDEV DLLGYMNILE EARDRGRKYG TSLMLFYQST GQLVNHFGES GARSWFESCS FVSYAAIKDL QTAKEISERC GQMTVEVTGV SKSRGLSLGK SSYNINYQQR ALILPHEVIQ EMRQDEQIIL MQGQPPLRCG RAIYFRRKEM LAASAKNRFA PQKKN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata aggtcaagcg tgccaaaaaa gaggtttttg ggggagcggc atggatggat ttaagagaag cagcaaaaat ctttcctgcc gatgggcaaa ttgttgtggg ggaaagatac cgtgttgatc aagataatgt atgcaaaatt ccatttgcac ctggcaataa aacaacatgg gggaaaggtg gagcagtacc tttgctaacc tttaatcttg attttggttc aacccatatg atcttttttg ctggttctgg tggttataaa accacaagta cagtaattcc tacgtgttta acctatccag ggtctattat ttgtcttgat ccttcaacag aagttgcccc aatcgtcaaa tttgctcgtc aaaagatggg caatagaaat gttattattc ttgatcctaa ttcactttta acaaaaagtt ttaatgtcat cgattggctt ttagacgaca gtgtaccccg cacacaacgt gaagcaaata ttgttagctt ttccaaattg cttctcacgg ataaaaaatc agacaattct tcggcggaat atttctcgac acaagcccat aatcttttaa cggctcttct tgctcatgtg atattttctg atgaatatga agaccatgag cgcaacttaa aaacattgcg tggaattctc tctcaatcag aaacagctat tatcaatcaa ttacgcatga ttcaagaaac aaccccttct ccttttattc gtgaaatggt gggtattttt acagaaatgg cagaacaaac tttttcagga gtctatacaa ctgcctcaaa agacacccaa tggctttctt tgtctaatta cgcagatctt gtctgcggtg atgatttttc ctcctcagat attgccaatg gtaatataga tgtttttctc aatctccccg ccagcatttt aaacagctat ccagcgatag gacgtgtaat tattggtgcc tttctcaatg ctatggttac agcagacggt aactataaaa agcgtgtttt atttgtctta gatgaagttg atcttctagg ctatatgaat attttagaag aagctcgtga tcgtggacgt aaatacggca catccttaat gctcttttat caatccacgg gtcagttggt caatcacttt ggtgaatcag gggcacgttc atggtttgaa agctgctcct ttgtcagtta tgctgccatt aaagacctcc aaacagcgaa agagatttca gaacgctgtg gtcaaatgac cgttgaagtc accggagtaa gcaaatcaag aggtttatct ttaggaaaga gctcttacaa tatcaattac caacaaagag ctctcatttt accccacgag gttattcaag aaatgcgtca agatgaacaa attattctta tgcaagggca acctcccttg cgatgtggcc gtgccatcta tttcagaaga aaagaaatgt tagctgcttc tgcaaaaaat cgttttgccc cacaaaagaa aaactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BatrA.18398.a.B2.PS01672
PURIFICATION DATe: 11/7/2012
CONCENTRATION: 40mg/ml
OBSERVED MW: 54kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN KVKRAKKEVF GGAAWMDLRE AAKIFPADGQ IVVGERYRVD QDNVCKIPFA PGNKTTWGKG GAVPLLTFNL DFGSTHMIFF AGSGGYKTTS TVIPTCLTYP GSIICLDPST EVAPIVKFAR QKMGNRNVII LDPNSLLTKS FNVIDWLLDD SVPRTQREAN IVSFSKLLLT DKKSDNSSAE YFSTQAHNLL TALLAHVIFS DEYEDHERNL KTLRGILSQS ETAIINQLRM IQETTPSPFI REMVGIFTEM AEQTFSGVYT TASKDTQWLS LSNYADLVCG DDFSSSDIAN GNIDVFLNLP ASILNSYPAI GRVIIGAFLN AMVTADGNYK KRVLFVLDEV DLXGYXNILE EARDRGRKYG TSLMLFYQST GQLVNHFGES GARSWFESCS FVSYAAIKDL QTAKEISERC GQMTVEVTGV SKSRGLSLGK SSYNINYQQR ALILPHEVIQ EMRQDEQIIL MQGQPPLRCG RAIYFRRKEM LAASA
Validated NT Sequence
atggctcacc accaccacca ccatatgaat aaggtcaagc gtgccaaaaa agaggttttt gggggagcgg catggatgga tttaagagaa gcagcaaaaa tctttcctgc cgatgggcaa attgttgtgg gggaaagata ccgtgttgat caagataatg tatgcaaaat tccatttgca cctggcaata aaacaacatg ggggaaaggt ggagcagtac ctttgctaac ctttaatctt gattttggtt caacccatat gatctttttt gctggttctg gtggttataa aaccacaagt acagtaattc ctacgtgttt aacctatcca gggtctatta tttgtcttga tccttcaaca gaagttgccc caatcgtcaa atttgctcgt caaaagatgg gcaatagaaa tgttattatt cttgatccta attcactttt aacaaaaagt tttaatgtca tcgattggct tttagacgac agtgtacccc gcacacaacg tgaagcaaat attgttagct tttccaaatt gcttctcacg gataaaaaat cagacaattc ttcggcggaa tatttctcga cacaagccca taatctttta acggctcttc ttgctcatgt gatattttct gatgaatatg aagaccatga gcgcaactta aaaacattgc gtggaattct ctctcaatca gaaacagcta ttatcaatca attacgcatg attcaagaaa caaccccttc tccttttatt cgtgaaatgg tgggtatttt tacagaaatg gcagaacaaa ctttttcagg agtctataca actgcctcaa aagacaccca atggctttct ttgtctaatt acgcagatct tgtctgcggt gatgattttt cctcctcaga tattgccaat ggtaatatag atgtttttct caatctcccc gccagcattt taaacagcta tccagcgata ggacgtgtaa ttattggtgc ctttctcaat gctatggtta cagcagacgg taactataaa aagcgtgttt tatttgtctt agatgaagtt gatctnntng gctatnngaa tattttagaa gaagctcgtg atcgtggacg taaatacggc acatccttaa tgctctttta tcaatccacg ggtcagttgg tcaatcactt tggtgaatca ggggcacgtt catggtttga aagctgctcc tttgtcagtt atgctgccat taaagacctc caaacagcga aagagatttc agaacgctgt ggtcaaatga ccgttgaagt caccggagta agcaaatcaa gaggtttatc tttaggaaag agctcttaca atatcaatta ccaacaaaga gctctcattt taccccacga ggttattcaa gaaatgcgtc aagatgaaca aattattctt atgcaagggc aacctccctt gcgatgtggc cgtgccatct atttcagaag aaaagaaatg ttagctgctt ctgcaaa
Expressed Protein Sequence
MAHHHHHHMN KVKRAKKEVF GGAAWMDLRE AAKIFPADGQ IVVGERYRVD QDNVCKIPFA PGNKTTWGKG GAVPLLTFNL DFGSTHMIFF AGSGGYKTTS TVIPTCLTYP GSIICLDPST EVAPIVKFAR QKMGNRNVII LDPNSLLTKS FNVIDWLLDD SVPRTQREAN IVSFSKLLLT DKKSDNSSAE YFSTQAHNLL TALLAHVIFS DEYEDHERNL KTLRGILSQS ETAIINQLRM IQETTPSPFI REMVGIFTEM AEQTFSGVYT TASKDTQWLS LSNYADLVCG DDFSSSDIAN GNIDVFLNLP ASILNSYPAI GRVIIGAFLN AMVTADGNYK KRVLFVLDEV DLLGYMNILE EARDRGRKYG TSLMLFYQST GQLVNHFGES GARSWFESCS FVSYAAIKDL QTAKEISERC GQMTVEVTGV SKSRGLSLGK SSYNINYQQR ALILPHEVIQ EMRQDEQIIL MQGQPPLRCG RAIYFRRKEM LAASAKNRFA PQKKN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATA AGGTCAAGCG TGCCAAAAAA GAGGTTTTTG GGGGAGCGGC ATGGATGGAT TTAAGAGAAG CAGCAAAAAT CTTTCCTGCC GATGGGCAAA TTGTTGTGGG GGAAAGATAC CGTGTTGATC AAGATAATGT ATGCAAAATT CCATTTGCAC CTGGCAATAA AACAACATGG GGGAAAGGTG GAGCAGTACC TTTGCTAACC TTTAATCTTG ATTTTGGTTC AACCCATATG ATCTTTTTTG CTGGTTCTGG TGGTTATAAA ACCACAAGTA CAGTAATTCC TACGTGTTTA ACCTATCCAG GGTCTATTAT TTGTCTTGAT CCTTCAACAG AAGTTGCCCC AATCGTCAAA TTTGCTCGTC AAAAGATGGG CAATAGAAAT GTTATTATTC TTGATCCTAA TTCACTTTTA ACAAAAAGTT TTAATGTCAT CGATTGGCTT TTAGACGACA GTGTACCCCG CACACAACGT GAAGCAAATA TTGTTAGCTT TTCCAAATTG CTTCTCACGG ATAAAAAATC AGACAATTCT TCGGCGGAAT ATTTCTCGAC ACAAGCCCAT AATCTTTTAA CGGCTCTTCT TGCTCATGTG ATATTTTCTG ATGAATATGA AGACCATGAG CGCAACTTAA AAACATTGCG TGGAATTCTC TCTCAATCAG AAACAGCTAT TATCAATCAA TTACGCATGA TTCAAGAAAC AACCCCTTCT CCTTTTATTC GTGAAATGGT GGGTATTTTT ACAGAAATGG CAGAACAAAC TTTTTCAGGA GTCTATACAA CTGCCTCAAA AGACACCCAA TGGCTTTCTT TGTCTAATTA CGCAGATCTT GTCTGCGGTG ATGATTTTTC CTCCTCAGAT ATTGCCAATG GTAATATAGA TGTTTTTCTC AATCTCCCCG CCAGCATTTT AAACAGCTAT CCAGCGATAG GACGTGTAAT TATTGGTGCC TTTCTCAATG CTATGGTTAC AGCAGACGGT AACTATAAAA AGCGTGTTTT ATTTGTCTTA GATGAAGTTG ATCTTCTAGG CTATATGAAT ATTTTAGAAG AAGCTCGTGA TCGTGGACGT AAATACGGCA CATCCTTAAT GCTCTTTTAT CAATCCACGG GTCAGTTGGT CAATCACTTT GGTGAATCAG GGGCACGTTC ATGGTTTGAA AGCTGCTCCT TTGTCAGTTA TGCTGCCATT AAAGACCTCC AAACAGCGAA AGAGATTTCA GAACGCTGTG GTCAAATGAC CGTTGAAGTC ACCGGAGTAA GCAAATCAAG AGGTTTATCT TTAGGAAAGA GCTCTTACAA TATCAATTAC CAACAAAGAG CTCTCATTTT ACCCCACGAG GTTATTCAAG AAATGCGTCA AGATGAACAA ATTATTCTTA TGCAAGGGCA ACCTCCCTTG CGATGTGGCC GTGCCATCTA TTTCAGAAGA AAAGAAATGT TAGCTGCTTC TGCAAAAAAT CGTTTTGCCC CACAAAAGAA AAACTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT