BatrA.18388.c

VirB8 homologue (VirB type IV secretion machinery)

CENTER ID: BatrA.18388.c
ORGANISM: Bartonella tribocorum CIP 105476 / IBS 506
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BatrA.18388.c.B2.GE35756 soluble domain 65 234
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: Q8GJ60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKHNLIKLWR LRVKSDALEE YIKEARSFDI DRMHGMRVRM KISMALTVLF GLMTIALALA VAALTPLKTV EPFVIRVDNS TGIIDTVSAL KESPSDYDEA ITRYFASQYV RAREGFQASE AENSFRLVSL LSSPKEQNRF AKWYAGNNPE SPQNIYHNMI ATVTIKSISF ISKDLIQVRY YKTVRDFSEK ETISHWVSIL NFSYVNAHIS TSDRLINPLG FQVSEYRSDP EVIK
NT Sequence
atgaagcaca atttgataaa actgtggagg ttacgcgtga aaagtgatgc ccttgaagaa tatataaaag aagcacgctc atttgacatt gatcgcatgc atggcatgcg cgtgcggatg aaaatttcca tggctttaac agtgcttttt ggattgatga caattgcgct cgctttggct gtcgcagctt tgacgccctt gaaaactgta gaaccttttg ttatccgcgt tgataactca acaggcatca tcgatactgt aagtgcccta aaagaatccc ctagcgacta tgatgaagcg ataacgcgtt attttgctag ccaatatgtt cgtgctcgtg aaggttttca agcatcagag gcagaaaaca gttttcgctt agtctctctt ttatcttctc caaaagaaca aaatcgtttt gcaaaatggt acgcgggcaa taatccagaa agtccacaaa atatttatca taacatgatt gctacagtca caatcaaatc aatctctttt ataagcaaag atcttatcca agttcgttac tataaaacgg tcagagactt tagtgaaaaa gaaactattt cccattgggt ttcaatctta aatttttctt atgttaacgc acacatttca acctctgacc gtctcatcaa cccccttggt tttcaagtat cggaatatag atctgatcca gaggtgataa aatga
Details for BatrA.18388.c.B2.GE35756
HARVESTED ON: 7/31/2012
SEQUENCED ON: 8/6/2012
EXPECTED MW: 20kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT PLKTVEPFVI RVDNSTGIID TVSALKESPS DYDEAITRYF ASQYVRAREG FQASEAENSF RLVSLLSSPK EQNRFAKWYA GNNPESPQNI YHNMIATVTI KSISFISKDL IQVRYYKTVR DFSEKETISH WVSILNFSYV NAHISTSDRL INPLGFQVSE YRSDPEVIK
Validated NT Sequence
atggctcacc accaccacca ccatatgacg cccttgaaaa ctgtagaacc ttttgttatc cgcgttgata actcaacagg catcatcgat actgtaagtg ccctaaaaga atcccctagc gactatgatg aagcgataac gcgttatttt gctagccaat atgttcgtgc tcgtgaaggt tttcaagcat cagaggcaga aaacagtttt cgcttagtct ctcttttatc ttctccaaaa gaacaaaatc gttttgcaaa atggtacgcg ggcaataatc cagaaagtcc acaaaatatt tatcataaca tgattgctac agtcacaatc aaatcaatct cttttataag caaagatctt atccaagttc gttactataa aacggtcaga gactttagtg aaaaagaaac tatttcccat tgggtttcaa tcttaaattt ttcttatgtt aacgcacaca tttcaacctc tgaccgtctc atcaaccccc ttggttttca agtatcggaa tatagatctg atccagaggt gataaaataa gtgagtaaga taggatccgg ctgct
Expected Protein Sequence
MAHHHHHHMT PLKTVEPFVI RVDNSTGIID TVSALKESPS DYDEAITRYF ASQYVRAREG FQASEAENSF RLVSLLSSPK EQNRFAKWYA GNNPESPQNI YHNMIATVTI KSISFISKDL IQVRYYKTVR DFSEKETISH WVSILNFSYV NAHISTSDRL INPLGFQVSE YRSDPEVIK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgc ccttgaaaac tgtagaacct tttgttatcc gcgttgataa ctcaacaggc atcatcgata ctgtaagtgc cctaaaagaa tcccctagcg actatgatga agcgataacg cgttattttg ctagccaata tgttcgtgct cgtgaaggtt ttcaagcatc agaggcagaa aacagttttc gcttagtctc tcttttatct tctccaaaag aacaaaatcg ttttgcaaaa tggtacgcgg gcaataatcc agaaagtcca caaaatattt atcataacat gattgctaca gtcacaatca aatcaatctc ttttataagc aaagatctta tccaagttcg ttactataaa acggtcagag actttagtga aaaagaaact atttcccatt gggtttcaat cttaaatttt tcttatgtta acgcacacat ttcaacctct gaccgtctca tcaaccccct tggttttcaa gtatcggaat atagatctga tccagaggtg ataaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t