BatrA.17987.a

DNA polymerase III subunit beta (EC 2.7.7.7)

CENTER ID: BatrA.17987.a
ORGANISM: Bartonella tribocorum CIP 105476 / IBS 506
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BatrA.17987.a.B1.GE41241 full length 1 373
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|382640.5.peg.152
UniProt: A9ILZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRITVDRNQF LKSLGRVHRV VERRNTVPIL SNVLIDAENG HVQLKTTDLD LEVTESFTVN VEHAGATTVP AHLLYDIIRK LPDGNEIVLS VDENQASTMS VVSGCVHFQL QCLPKADFPE SLPGQFGYRF SLSASGLKHL LDCTQFAIST EETRYYLNGI YFHVINDDVL KLRLVATDGH RLAQVEMEAP SGVEGMPGVI VPRKAVGELQ KLLSEENDGD VCIELSETKI RFSVGSVIFT SKLIDGTFPD YQRVIPLGND KELVVNRQDF SSAVDRVSTI SSDRGRAVKL TIEHGQLRLV VNSPDSGSAE DQLSVTYSSE PLEIGFNSRY LLDIAGQLSS DEMVFMLAEA GAPALIRDND DADVLYVLMP IRV
NT Sequence
atgcgtatta cagtggatcg caatcaattt ctcaagtctc ttggtcgtgt tcaccgtgtg gtagaacgcc gtaatactgt tcctatttta tcgaatgtgc tcattgatgc agaaaatggc catgtgcagt taaagacaac agatcttgat ttagaggtta cagaatcctt tacagtcaat gtcgagcacg ctggagcaac cactgttccg gcacatttgc tctatgatat cattcggaaa cttcctgatg gcaatgaaat tgtattatcc gtcgatgaga atcaagcaag tacaatgtcc gttgtttcgg gttgtgtaca tttccaactt caatgtctgc caaaagcaga ttttccagag tcgcttcctg gacagtttgg ttatcgcttt tctctgtctg catctgggtt gaaacattta cttgattgta cgcaatttgc catttctact gaagaaaccc gctattatct gaatggtatt tacttccatg ttattaatga tgatgttttg aagctgcgtt tggttgcaac tgatggtcat cgtttggcac aagttgagat ggaagctcct tcaggcgtcg aaggaatgcc cggtgttatc gttcctcgta aagctgtagg agagttgcaa aaacttcttt cagaagaaaa tgatggtgat gtatgtatag agctttcaga gacaaagatt cgtttttctg taggttctgt gatttttaca tcaaagttaa ttgatggaac atttccagat tatcaacgtg ttattcctct tggcaatgat aaagaattag ttgttaatag acaggatttt tcttctgcag ttgatcgtgt ttcaacgatt tcaagtgatc gggggcgtgc agttaagtta acgattgagc atggacagtt aaggcttgtt gttaatagtc ctgattcggg tagtgcggaa gatcaattat ccgtaactta ctcatctgaa ccgcttgaga tagggtttaa ttcacgttat cttttagata ttgctgggca gctttcaagc gatgagatgg ttttcatgtt ggctgaagct ggagcgccag ctctgattcg tgataatgat gatgcggatg tactctatgt gctgatgcct attcgcgttt ag
Details for BatrA.17987.a.B1.GE41241
HARVESTED ON: 3/8/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 42kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMX XTVDRNQFLK SLGRVHRVVE RRNTVPILSN VLIDAENGHV QLKTTDLDLE VTESFTVNVE HAGATTVPAH LLYDIIRKLP DGNEIVLSVD ENQASTMSVV SGCVHFQLQC LPKADFPESL PGQFGYRFSL SASGLKHLLD CTQFAISTEE TRYYLNGIYF HVINDDVLKL RLVATDGHRL AQVEMEAPSG VEGMPGVIVP RKAVGELQKL LSEENDGDVC IELSETKIRF SVGSVIFTSK LIDGTFPDYQ RVIPLGNDKE LVVNRQDFSS AVDRVSTISS DRGRAVKLTI EHGQLRLVVN SPDSGSAEDQ LSVTYSSEPL EIGFNSRYLL DIAGQLSSDE MVFMLAEAGA PALIRDNDDA DV
Validated NT Sequence
atggctcacc accaccacca ccatatgnnn nntacagtgg atcgcaatca atttctcaag tctcttggtc gtgttcaccg tgtggtagaa cgccgtaata ctgttcctat tttatcgaat gtgctcattg atgcagaaaa tggccatgtg cagttaaaga caacagatct tgatttagag gttacagaat cctttacagt caatgtcgag cacgctggag caaccactgt tccggcacat ttgctctatg atatcattcg gaaacttcct gatggcaatg aaattgtatt atccgtcgat gagaatcaag caagtacaat gtccgttgtt tcgggttgtg tacatttcca acttcaatgt ctgccaaaag cagattttcc agagtcgctt cctggacagt ttggttatcg cttttctctg tctgcatctg ggttgaaaca tttacttgat tgtacgcaat ttgccatttc tactgaagaa acccgctatt atctgaatgg tatttacttc catgttatta atgatgatgt tttgaagctg cgtttggttg caactgatgg tcatcgtttg gcacaagttg agatggaagc tccttcaggc gtcgaaggaa tgcccggtgt tatcgttcct cgtaaagctg taggagagtt gcaaaaactt ctttcagaag aaaatgatgg tgatgtatgt atagagcttt cagagacaaa gattcgtttt tctgtaggtt ctgtgatttt tacatcaaag ttaattgatg gaacatttcc agattatcaa cgtgttattc ctcttggcaa tgataaagaa ttagttgtta atagacagga tttttcttct gcagttgatc gtgtttcaac gatttcaagt gatcgggggc gtgcagttaa gttaacgatt gagcatggac agttaaggct tgttgttaat agtcctgatt cgggtagtgc ggaagatcaa ttatccgtaa cttactcatc tgaaccgctt gagatagggt ttaattcacg ttatctttta gatattgctg ggcagctttc aagcgatgag atggttttca tgttggctga agctggagcg ccagctctga ttcgtgataa tgatgatgcg gatgtac
Expected Protein Sequence
MAHHHHHHMR ITVDRNQFLK SLGRVHRVVE RRNTVPILSN VLIDAENGHV QLKTTDLDLE VTESFTVNVE HAGATTVPAH LLYDIIRKLP DGNEIVLSVD ENQASTMSVV SGCVHFQLQC LPKADFPESL PGQFGYRFSL SASGLKHLLD CTQFAISTEE TRYYLNGIYF HVINDDVLKL RLVATDGHRL AQVEMEAPSG VEGMPGVIVP RKAVGELQKL LSEENDGDVC IELSETKIRF SVGSVIFTSK LIDGTFPDYQ RVIPLGNDKE LVVNRQDFSS AVDRVSTISS DRGRAVKLTI EHGQLRLVVN SPDSGSAEDQ LSVTYSSEPL EIGFNSRYLL DIAGQLSSDE MVFMLAEAGA PALIRDNDDA DVLYVLMPIR V
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgta ttacagtgga tcgcaatcaa tttctcaagt ctcttggtcg tgttcaccgt gtggtagaac gccgtaatac tgttcctatt ttatcgaatg tgctcattga tgcagaaaat ggccatgtgc agttaaagac aacagatctt gatttagagg ttacagaatc ctttacagtc aatgtcgagc acgctggagc aaccactgtt ccggcacatt tgctctatga tatcattcgg aaacttcctg atggcaatga aattgtatta tccgtcgatg agaatcaagc aagtacaatg tccgttgttt cgggttgtgt acatttccaa cttcaatgtc tgccaaaagc agattttcca gagtcgcttc ctggacagtt tggttatcgc ttttctctgt ctgcatctgg gttgaaacat ttacttgatt gtacgcaatt tgccatttct actgaagaaa cccgctatta tctgaatggt atttacttcc atgttattaa tgatgatgtt ttgaagctgc gtttggttgc aactgatggt catcgtttgg cacaagttga gatggaagct ccttcaggcg tcgaaggaat gcccggtgtt atcgttcctc gtaaagctgt aggagagttg caaaaacttc tttcagaaga aaatgatggt gatgtatgta tagagctttc agagacaaag attcgttttt ctgtaggttc tgtgattttt acatcaaagt taattgatgg aacatttcca gattatcaac gtgttattcc tcttggcaat gataaagaat tagttgttaa tagacaggat ttttcttctg cagttgatcg tgtttcaacg atttcaagtg atcgggggcg tgcagttaag ttaacgattg agcatggaca gttaaggctt gttgttaata gtcctgattc gggtagtgcg gaagatcaat tatccgtaac ttactcatct gaaccgcttg agatagggtt taattcacgt tatcttttag atattgctgg gcagctttca agcgatgaga tggttttcat gttggctgaa gctggagcgc cagctctgat tcgtgataat gatgatgcgg atgtactcta tgtgctgatg cctattcgcg tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat