BatrA.17330.f

BepF protein (VirB-translocated Bartonella effector protein)

CENTER ID: BatrA.17330.f
ORGANISM: Bartonella tribocorum CIP 105476 / IBS 506
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BatrA.17330.f.B1.GE35955 full length 1 655
BatrA.17330.f.B3.GE37288 BID2 475 613
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|382640.5.peg.1771
RefSeq: YP_001610015.1
UniProt: A9IWQ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKRNPSPLI AEMMAKFQQQ AEQSPTSPSH SQVSQSVPKP PVQKRPENLS HPPKVAPPPP LRMRDKTPAD TKIQKPETQE SSSAHIKTPP QRPPRAKDRE QNKQTAQESP SLQTKMAPPK PPRIKDKTPA DTKIQKPETQ ESSSAHIKTP PQRPPRAKDR EQNKQTAQES PSLQTKMAPP KPPRIKDKTP ADTKIQKPET QESSSAHIKT PPPKPPRAKD REQNKQTAQE SPSLQTKMPP PTPPRIKDKT PANATIQVQT STKNQNLETI APSVRPKTPQ HRQVRQQSSS SSQNSESYAV PPSRKPHQIG DRQKSEAAIQ KTAIRQPKPS QERKASINTM EKKLLLEAYH EEIRFLCKKT FGDRLILESR IEAIKENPNM GEQLLWDVTK KPQSISKLAG RKVLGIKNGA RKQAEETLSP LYAAIEGFVY TAKYVQENTS QNSHTKQQQH QQAQQEGAIS QHARNPLHSE KKIQPLSNQE IARRVQQNPS VQYGQREVQY WCQKVYRDPF IFNDRMEDMQ KIPEIGEELV WQIENHSQLF TPLAGKKVLG VKNEARKTAE ESLSTLCTAI KDYADTIKQV RESIVLHNQE QQKHHQPLSE IDKKLQKQQS LSQSAKPLER STVKQHKELT EASKQEERPP IRPRKAETSK TMALS
NT Sequence
atgaaaaaaa gaaacccctc ccctttaata gccgaaatga tggcaaaatt tcaacaacag gctgaacaat ctcccaccag tccctcccat tcccaagttt ctcaaagtgt gccaaaacca cctgtacaaa aacgcccaga gaatttgtca catcccccaa aggttgcgcc accgcccccc ctacgtatga gagataaaac accagctgat acaaaaatcc aaaagcctga aacccaagaa agctcctccg cgcacataaa aacgcctcca caaagacctc cacgtgcaaa agatagagaa caaaacaaac aaacggctca agaatccccc tctctacaga caaaaatggc tccgcccaaa cctccacgca taaaagataa aacaccagcc gacacaaaaa tccaaaagcc tgaaacccaa gaaagctcct ccgcgcacat aaaaacgcct ccacaaagac ctccacgtgc gaaggataga gaacaaaaca aacaaacggc tcaagaatcc ccctctctac agacaaaaat ggctccgccc aaacctccac gcataaaaga taaaacacca gccgacacaa aaatccaaaa gcctgaaacc caagaaagct cctccgcaca cataaaaacg cctccaccaa aacctccacg tgcaaaagat agagaacaaa acaaacaaac ggctcaagaa tccccctctc tacagacaaa aatgcctccg cccacccccc cacgcataaa agataaaaca ccagccaacg caacaatcca agtgcaaacc agcacaaaaa accaaaatct agaaaccatt gctccaagtg ttagacccaa aacaccacag cacaggcaag tcaggcagca aagcagttca tcatcacaaa acagtgagtc atatgcagta cctccttcaa gaaaaccaca ccaaatagga gacagacaaa aaagtgaggc tgccatccaa aaaacagcaa ttagacaacc aaaaccgtct caggagcgta aagcatctat taatacaatg gaaaaaaagc tattgctcga agcataccat gaagaaatac gatttttatg taaaaagact tttggtgacc gactcatttt agaatcaaga atagaagcca tcaaagaaaa ccccaatatg ggagaacaac tcttatggga tgttacaaaa aaacctcagt ctatttctaa gcttgctggt agaaaagttc ttggcataaa aaatggagcg cgcaaacaag ctgaagaaac gctttccccc ctatatgctg ccatagaagg ctttgtatat actgcaaaat atgtacaaga gaatacttca caaaattctc acacaaaaca acaacagcat caacaagcgc aacaggaagg agcaattagt caacacgcac gaaatcctct ccattcagaa aagaaaatcc agcctctctc aaatcaagaa attgcccgca gagttcaaca aaacccatcg gttcaatatg gtcaaagaga agtccaatat tggtgtcaaa aagtttatag agacccattt atttttaacg acagaatgga agatatgcaa aaaattccag agataggaga agagctcgta tggcaaattg aaaaccattc tcagcttttt actccacttg ctggtaaaaa agtccttggt gtaaaaaacg aagcacgcaa aactgctgaa gaaagtcttt ccaccctttg taccgccatt aaggattatg cagacactat aaaacaggta agagaaagta tcgtgctgca caatcaagaa caacaaaaac accatcagcc tcttagcgaa atagataaaa aattacaaaa acagcaaagc ctatcgcagt ccgcaaaacc acttgaacgc tcaacagtaa agcagcataa agagcttact gaagcatcga aacaagaaga gcgaccgcct attcgaccac gcaaagccga aacatcaaaa acaatggctt tatcctga
Details for BatrA.17330.f.B1.GE35955
HARVESTED ON: 8/28/2012
SEQUENCED ON: 2/22/2016
EXPECTED MW: 75kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 46
Validated AA Sequence
MAHHHHHHMK KRNPSPLIAE MMAKFQQQAE QSPTSPSHSQ VSQSVPKPPV QKRPENLSHP PKVAPPPPLR MRDKTPADTK IQKPETQESS SAHIKTPPQR PPRAKDREQN KQTAQESPSL QTKMAPPKPP RIKDKTPADT KIQKPETQES SSAHIKTPPQ RPPRAKDREQ NKQTAQESPS LQTKMAPPKP PRIKDKTPAD TKIQKPETQE SSSAHIKTPP PKPPRAKDRE QNKQTAQESP SLQTKMPPPT PPRIKDKTPA NATIQVQTST KXQNLETIAP SVRPKTPQHR QVXXQSSSSS QXXEXXXXXX
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa aaaagaaacc cctccccttt aatagccgaa atgatggcaa aatttcaaca acaggctgaa caatctccca ccagtccctc ccattcccaa gtttctcaaa gtgtgccaaa accacctgta caaaaacgcc cagagaattt gtcacatccc ccaaaggttg cgccaccgcc ccccctacgt atgagagata aaacaccagc tgatacaaaa atccaaaagc ctgaaaccca agaaagctcc tccgcgcaca taaaaacgcc tccacaaaga cctccacgtg caaaagatag agaacaaaac aaacaaacgg ctcaagaatc cccctctcta cagacaaaaa tggctccgcc caaacctcca cgcataaaag ataaaacacc agccgacaca aaaatccaaa agcctgaaac ccaagaaagc tcctccgcgc acataaaaac gcctccacaa agacctccac gtgcgaagga tagagaacaa aacaaacaaa cggctcaaga atccccctct ctacagacaa aaatggctcc gcccaaacct ccacgcataa aagataaaac accagccgac acaaaaatcc aaaagcctga aacccaagaa agctcctccg cacacataaa aacgcctcca ccaaaacctc cacgtgcaaa agatagagaa caaaacaaac aaacggctca agaatccccc tctctacaga caaaaatgcc tccgcccacc cccccacgca taaaagataa aacaccagcc aacgcaacaa tccaagtgca aaccagcaca aaanaccaaa atctagaaac cattgctcca agtgttagac ccaaaacacc acagcacagg caagtcagnn nncaaagcag ttcatcatca caaannnntg agncntnnnn nnnnnnnnct tc
Expected Protein Sequence
MAHHHHHHMK KRNPSPLIAE MMAKFQQQAE QSPTSPSHSQ VSQSVPKPPV QKRPENLSHP PKVAPPPPLR MRDKTPADTK IQKPETQESS SAHIKTPPQR PPRAKDREQN KQTAQESPSL QTKMAPPKPP RIKDKTPADT KIQKPETQES SSAHIKTPPQ RPPRAKDREQ NKQTAQESPS LQTKMAPPKP PRIKDKTPAD TKIQKPETQE SSSAHIKTPP PKPPRAKDRE QNKQTAQESP SLQTKMPPPT PPRIKDKTPA NATIQVQTST KNQNLETIAP SVRPKTPQHR QVRQQSSSSS QNSESYAVPP SRKPHQIGDR QKSEAAIQKT AIRQPKPSQE RKASINTMEK KLLLEAYHEE IRFLCKKTFG DRLILESRIE AIKENPNMGE QLLWDVTKKP QSISKLAGRK VLGIKNGARK QAEETLSPLY AAIEGFVYTA KYVQENTSQN SHTKQQQHQQ AQQEGAISQH ARNPLHSEKK IQPLSNQEIA RRVQQNPSVQ YGQREVQYWC QKVYRDPFIF NDRMEDMQKI PEIGEELVWQ IENHSQLFTP LAGKKVLGVK NEARKTAEES LSTLCTAIKD YADTIKQVRE SIVLHNQEQQ KHHQPLSEID KKLQKQQSLS QSAKPLERST VKQHKELTEA SKQEERPPIR PRKAETSKTM ALS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aaagaaaccc ctccccttta atagccgaaa tgatggcaaa atttcaacaa caggctgaac aatctcccac cagtccctcc cattcccaag tttctcaaag tgtgccaaaa ccacctgtac aaaaacgccc agagaatttg tcacatcccc caaaggttgc gccaccgccc cccctacgta tgagagataa aacaccagct gatacaaaaa tccaaaagcc tgaaacccaa gaaagctcct ccgcgcacat aaaaacgcct ccacaaagac ctccacgtgc aaaagataga gaacaaaaca aacaaacggc tcaagaatcc ccctctctac agacaaaaat ggctccgccc aaacctccac gcataaaaga taaaacacca gccgacacaa aaatccaaaa gcctgaaacc caagaaagct cctccgcgca cataaaaacg cctccacaaa gacctccacg tgcgaaggat agagaacaaa acaaacaaac ggctcaagaa tccccctctc tacagacaaa aatggctccg cccaaacctc cacgcataaa agataaaaca ccagccgaca caaaaatcca aaagcctgaa acccaagaaa gctcctccgc acacataaaa acgcctccac caaaacctcc acgtgcaaaa gatagagaac aaaacaaaca aacggctcaa gaatccccct ctctacagac aaaaatgcct ccgcccaccc ccccacgcat aaaagataaa acaccagcca acgcaacaat ccaagtgcaa accagcacaa aaaaccaaaa tctagaaacc attgctccaa gtgttagacc caaaacacca cagcacaggc aagtcaggca gcaaagcagt tcatcatcac aaaacagtga gtcatatgca gtacctcctt caagaaaacc acaccaaata ggagacagac aaaaaagtga ggctgccatc caaaaaacag caattagaca accaaaaccg tctcaggagc gtaaagcatc tattaataca atggaaaaaa agctattgct cgaagcatac catgaagaaa tacgattttt atgtaaaaag acttttggtg accgactcat tttagaatca agaatagaag ccatcaaaga aaaccccaat atgggagaac aactcttatg ggatgttaca aaaaaacctc agtctatttc taagcttgct ggtagaaaag ttcttggcat aaaaaatgga gcgcgcaaac aagctgaaga aacgctttcc cccctatatg ctgccataga aggctttgta tatactgcaa aatatgtaca agagaatact tcacaaaatt ctcacacaaa acaacaacag catcaacaag cgcaacagga aggagcaatt agtcaacacg cacgaaatcc tctccattca gaaaagaaaa tccagcctct ctcaaatcaa gaaattgccc gcagagttca acaaaaccca tcggttcaat atggtcaaag agaagtccaa tattggtgtc aaaaagttta tagagaccca tttattttta acgacagaat ggaagatatg caaaaaattc cagagatagg agaagagctc gtatggcaaa ttgaaaacca ttctcagctt tttactccac ttgctggtaa aaaagtcctt ggtgtaaaaa acgaagcacg caaaactgct gaagaaagtc tttccaccct ttgtaccgcc attaaggatt atgcagacac tataaaacag gtaagagaaa gtatcgtgct gcacaatcaa gaacaacaaa aacaccatca gcctcttagc gaaatagata aaaaattaca aaaacagcaa agcctatcgc agtccgcaaa accacttgaa cgctcaacag taaagcagca taaagagctt actgaagcat cgaaacaaga agagcgaccg cctattcgac cacgcaaagc cgaaacatca aaaacaatgg ctttatcctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BatrA.17330.f.B3.GE37288
HARVESTED ON: 8/28/2013
SEQUENCED ON: 9/3/2013
EXPECTED MW: 17kDa
OBSERVED MW: 16kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP LSNQEIARRV QQNPSVQYGQ REVQYWCQKV YRDPFIFNDR MEDMQKIPEI GEELVWQIEN HSQLFTPLAG KKVLGVKNEA RKTAEESLST LCTAIKDYAD TIKQVRESIV LHNQEQQKHH QPLSEIDKKL QKQQSLSQ
Validated NT Sequence
atcttactca cttactgcga taggctttgc tgtttttgta attttttatc tatttcgcta agaggctgat ggtgtttttg ttgttcttga ttgtgcagca cgatactttc tcttacctgt tttatagtgt ctgcataatc cttaatggcg gtacaaaggg tggaaagact ttcttcagca gttttgcgtg cttcgttttt tacaccaagg acttttttac cagcaagtgg agtaaaaagc tgagaatggt tttcaatttg ccatacgagc tcttctccta tctctggaat tttttgcata tcttccattc tgtcgttaaa aataaatggg tctctataaa ctttttgaca ccaatattgg acttctcttt gaccatattg aaccgatggg ttttgttgaa ctctgcgggc aatttcttga tttgagagag gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHPL SNQEIARRVQ QNPSVQYGQR EVQYWCQKVY RDPFIFNDRM EDMQKIPEIG EELVWQIENH SQLFTPLAGK KVLGVKNEAR KTAEESLSTL CTAIKDYADT IKQVRESIVL HNQEQQKHHQ PLSEIDKKLQ KQQSLSQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcctctct caaatcaaga aattgcccgc agagttcaac aaaacccatc ggttcaatat ggtcaaagag aagtccaata ttggtgtcaa aaagtttata gagacccatt tatttttaac gacagaatgg aagatatgca aaaaattcca gagataggag aagagctcgt atggcaaatt gaaaaccatt ctcagctttt tactccactt gctggtaaaa aagtccttgg tgtaaaaaac gaagcacgca aaactgctga agaaagtctt tccacccttt gtaccgccat taaggattat gcagacacta taaaacaggt aagagaaagt atcgtgctgc acaatcaaga acaacaaaaa caccatcagc ctcttagcga aatagataaa aaattacaaa aacagcaaag cctatcgcag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat