SSGCID: Target Page

BatrA.17330.e

BepA protein (VirB-translocated Bep effector protein)

CENTER ID:	BatrA.17330.e
ORGANISM:	Bartonella tribocorum CIP 105476 / IBS 506
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BatrA.17330.e.B2.GE37284	FIC		16	233
BatrA.17330.e.B3.GE37285	FIC and truncated OB-Fold		16	299

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|382640.5.peg.1754
RefSeq:	YP_001610009.1
UniProt:	A9IWP4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPKAKEKTKN IETSSPHHYV YPNTTTLKNK YGITNFDAFT AKYTHDTAKA IVNLREAPLP EKFDSSYLCS IHHQLFKNTF EWAGQPRNVP FTFLDESVTA MPEMKRAEWN NVFANDDEIE EGLQTLNKTL AEKNALRSLT REDFISEAVP LFVSLKNIRP FINGNEHTIH LFFEKLAQAA EHQLDFSLVT KERMLVASVA AIQNDNLEPM QHLFEDISNP QKIHLLKEFM SNMKELGRDI NDRSVMVTKE GETYTGTYRG AGHDSLAFNV NGAYIIGDKE DLTPEQLKTL KPGDKFTFTA SQTQELKNIL IPKETLAPLS KTEKAEMIAK DACVHTARNQ IQRLSKTVYD NAKTLDKKMV AIIKNPALGQ QLAEQIERSP DSVAHLAGFS LCGLQNQARA NAKNHLEMLC SAVANFAHAV KHAEKEITQE HATEQKRRAT TVEMPSKSLQ DLFTLPKELQ QESLAKNLGL QKELSNLVKN INSRLSATEH KAVKNNDYET LAQSIGVSEN KAKQITQTVK QAKEVHQQAC TRTVNHSNVL AIAS

NT Sequence

atgccaaaag ccaaagaaaa aaccaaaaat atagagacat cttctcctca tcattatgtc tatccgaaca ccacaacact caaaaataaa tatggaataa caaactttga cgcttttact gcaaaatata cgcatgatac agcaaaagcc atagttaacc tgcgtgaagc ccccctacca gaaaaatttg atagctccta tctatgctct attcaccatc aattgtttaa aaataccttt gaatgggcag gacagccccg caatgttccc tttacatttt tagacgaaag cgttaccgcc atgccagaga tgaaaagggc ggaatggaac aatgtttttg cgaatgatga tgagattgaa gaaggtttac aaacattaaa taaaacactt gcagaaaaaa acgctttgag aagcttaacg cgcgaagatt ttatttcaga agccgtacca ctctttgtct ctctcaaaaa tatacgtcca ttcataaatg ggaatgaaca tacaatacac ctcttttttg aaaagcttgc tcaagcagca gagcatcagc ttgatttttc acttgttaca aaagaacgca tgctggttgc tagtgttgca gcaatacaaa atgacaatct agaaccgatg caacatctgt ttgaagatat ctccaatccc caaaaaattc atcttttaaa agaattcatg agcaatatga aagaacttgg acgcgatatt aatgatcgtt ctgttatggt cacaaaggaa ggtgaaactt acacgggcac ctataggggg gctggtcatg atagccttgc tttcaatgtg aatggcgctt acatcatcgg tgataaagaa gatctcacac cagaacaact caaaacatta aagcctggtg ataaatttac cttcactgcc tcacaaaccc aagagttaaa aaatattctc attcctaaag aaacattagc gcctttatca aaaactgaaa aagctgaaat gattgcaaaa gatgcctgtg tccacaccgc tcgaaaccaa atccaacgat tgtcaaaaac tgtttatgac aacgcaaaaa cattagacaa aaagatggtt gcgattatta aaaatccagc cttaggtcag caacttgcag agcagattga gaggtcaccc gactctgttg ctcatcttgc cggtttcagt ttatgtggac tccaaaatca ggcacgtgca aacgctaaaa atcatcttga gatgctctgt agtgctgttg caaatttcgc gcatgctgtc aaacatgcgg agaaagaaat tacgcaagaa catgcaacag aacagaaacg ccgtgcaacc acagtagaaa tgcccagtaa aagcttgcaa gatctcttca ccttgccaaa agaattacag caagaatctt tagcaaaaaa tcttggactt caaaaagagc tgagcaatct tgtaaaaaac atcaatagcc gcctctcagc aactgaacat aaagctgtca aaaataatga ttacgaaaca ttggctcaaa gtattggtgt ctcagaaaat aaagccaagc aaattacaca aaccgttaag caagccaaag aagtacacca gcaagcctgc acgcgcacag ttaaccattc aaatgtactc gctatagcca gctaa

Details for BatrA.17330.e.B2.GE37284

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP HHYVYPNTTT LKNKYGITNF DAFTAKYTHD TAKAIVNLRE APLPEKFDSS YLCSIHHQLF KNTFEWAGQP RNVPFTFLDE SVTAMPEMKR AEWNNVFAND DEIEEGLQTL NKTLAEKNAL RSLTREDFIS EAVPLFVSLK NIRPFINGNE HTIHLFFEKL AQAAEHQLDF SLVTKERMLV ASVAAIQNDN LEPMQHLFED ISXPQKIHLL KEFMSNM

Validated NT Sequence

attttgttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgcct catcattatg tctatccgaa caccacaaca ctcaaaaata aatatggaat aacaaacttt gacgctttta ctgcaaaata tacgcatgat acagcaaaag ccatagttaa cctgcgtgaa gcccccctac cagaaaaatt tgatagctcc tatctatgct ctattcacca tcaattgttt aaaaatacct ttgaatgggc aggacagccc cgcaatgttc cctttacatt tttagacgaa agcgttaccg ccatgccaga gatgaaaagg gcggaatgga acaatgtttt tgcgaatgat gatgagattg aagaaggttt acaaacatta aataaaacac ttgcagaaaa aaacgctttg agaagcttaa cgcgcgaaga ttttatttca gaagccgtac cactctttgt ctctctcaaa aatatacgtc cattcataaa tgggaatgaa catacaatac acctcttttt tgaaaagctt gctcaagcag cagagcatca gcttgatttt tcacttgtta caaaagaacg catgctggtt gctagtgttg cagcaataca aaatgacaat ctagaaccga tgcaacatct gtttgaagat atctccnatc cccaaaaaat tcatctttta aaagaattca tgagcaatat gtaagtgagt aagataggat ccggctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngnnnnnnnn annnnnnnnn nnnncggnna acncccncnn nna

Expected Protein Sequence

MAHHHHHHPH HYVYPNTTTL KNKYGITNFD AFTAKYTHDT AKAIVNLREA PLPEKFDSSY LCSIHHQLFK NTFEWAGQPR NVPFTFLDES VTAMPEMKRA EWNNVFANDD EIEEGLQTLN KTLAEKNALR SLTREDFISE AVPLFVSLKN IRPFINGNEH TIHLFFEKLA QAAEHQLDFS LVTKERMLVA SVAAIQNDNL EPMQHLFEDI SNPQKIHLLK EFMSNM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcctcatc attatgtcta tccgaacacc acaacactca aaaataaata tggaataaca aactttgacg cttttactgc aaaatatacg catgatacag caaaagccat agttaacctg cgtgaagccc ccctaccaga aaaatttgat agctcctatc tatgctctat tcaccatcaa ttgtttaaaa atacctttga atgggcagga cagccccgca atgttccctt tacattttta gacgaaagcg ttaccgccat gccagagatg aaaagggcgg aatggaacaa tgtttttgcg aatgatgatg agattgaaga aggtttacaa acattaaata aaacacttgc agaaaaaaac gctttgagaa gcttaacgcg cgaagatttt atttcagaag ccgtaccact ctttgtctct ctcaaaaata tacgtccatt cataaatggg aatgaacata caatacacct cttttttgaa aagcttgctc aagcagcaga gcatcagctt gatttttcac ttgttacaaa agaacgcatg ctggttgcta gtgttgcagc aatacaaaat gacaatctag aaccgatgca acatctgttt gaagatatct ccaatcccca aaaaattcat cttttaaaag aattcatgag caatatgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BatrA.17330.e.B3.GE37285

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	101

Validated AA Sequence

MAHHHHHHHH MPHHYVYPNT TTLKNKYGIT NFDAFTAKYT HDTAKAIVNL REAPLPEKFD SSYLCSIHHQ LFKNTFEWAG QPRNVPFTFL DESVTAMPEM KRAEWNNVFA NDDEIEEGLQ TLNKTLAEKN ALRSLTREDF ISEAVPLFVS LKNIRPFING NEHTIHLFFE KLAQAAEHQL DFSLVTKERM LVASVAAIQN DNLEPMQHLF EDISNPQKIH LLKEFMSNMK ELGRDINDRS VMVTKEGETY TGTYRGAGHD SLAFNVNGAY IIGDKEDLTP EQLKTLKPGD KFTFT

Validated NT Sequence

attttgtnnn ntttaagaag gaganatacc atggctcacc accaccacca ccaccaccat atgcctcatc attatgtcta tccgaacacc acaacactca aaaataaata tggaataaca aactttgacg cttttactgc aaaatatacg catgatacag caaaagccat agttaacctg cgtgaagccc ccctaccaga aaaatttgat agctcctatc tatgctctat tcaccatcaa ttgtttaaaa atacctttga atgggcagga cagccccgca atgttccctt tacattttta gacgaaagcg ttaccgccat gccagagatg aaaagggcgg aatggaacaa tgtttttgcg aatgatgatg agattgaaga aggtttacaa acattaaata aaacacttgc agaaaaaaac gctttgagaa gcttaacgcg cgaagatttt atttcagaag ccgtaccact ctttgtctct ctcaaaaata tacgtccatt cataaatggg aatgaacata caatacacct cttttttgaa aagcttgctc aagcagcaga gcatcagctt gatttttcac ttgttacaaa agaacgcatg ctggttgcta gtgttgcagc aatacaaaat gacaatctag aaccgatgca acatctgttt gaagatatct ccaatcccca aaaaattcat cttttaaaag aattcatgag caatatgaaa gaacttggac gcgatattaa tgatcgttct gttatggtca caaaggaagg tgaaacttac acgggcacct ataggggggc tggtcatgat agccttgctt tcaatgtgaa tggcgcttac atcatcggtg ataaagaaga tctcacacca gaacaactca aaacattaaa gcctggtgat aaatttacct tcacttaagt gagtaagana ggatcnnnnn nnnnnnnccc c

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcctcatc attatgtcta tccgaacacc acaacactca aaaataaata tggaataaca aactttgacg cttttactgc aaaatatacg catgatacag caaaagccat agttaacctg cgtgaagccc ccctaccaga aaaatttgat agctcctatc tatgctctat tcaccatcaa ttgtttaaaa atacctttga atgggcagga cagccccgca atgttccctt tacattttta gacgaaagcg ttaccgccat gccagagatg aaaagggcgg aatggaacaa tgtttttgcg aatgatgatg agattgaaga aggtttacaa acattaaata aaacacttgc agaaaaaaac gctttgagaa gcttaacgcg cgaagatttt atttcagaag ccgtaccact ctttgtctct ctcaaaaata tacgtccatt cataaatggg aatgaacata caatacacct cttttttgaa aagcttgctc aagcagcaga gcatcagctt gatttttcac ttgttacaaa agaacgcatg ctggttgcta gtgttgcagc aatacaaaat gacaatctag aaccgatgca acatctgttt gaagatatct ccaatcccca aaaaattcat cttttaaaag aattcatgag caatatgaaa gaacttggac gcgatattaa tgatcgttct gttatggtca caaaggaagg tgaaacttac acgggcacct ataggggggc tggtcatgat agccttgctt tcaatgtgaa tggcgcttac atcatcggtg ataaagaaga tctcacacca gaacaactca aaacattaaa gcctggtgat aaatttacct tcacttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat