BatrA.17330.a
BepC protein (VirB-translocated Bartonella effector protein)
| CENTER ID: | BatrA.17330.a |
| ORGANISM: | Bartonella tribocorum CIP 105476 / IBS 506 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BatrA.17330.a.B1.GE35950 | full length | 1 | 532 | ||
| BatrA.17330.a.B3.GE37279 | FIC and truncated OB-Fold | 3 | 287 | ||
| BatrA.17330.a.B2.GE37278 | FIC | 3 | 220 | ||
| BatrA.17330.a.B4.GE37280 | BID | 298 | 434 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party
can then use to order the clone from a third-party clone manufacturer.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BatrA.17330.a.B2.PS02120 | FIC | 3 | 220 | ||
| BatrA.17330.a.B4.PS01976 | BID | 298 | 434 |
Structures
4WGJ
DEPOSITED: 9/18/2014
DETERMINATION: XRay
CLONE: BatrA.17330.a.B2.GE37278
PROTEIN: BatrA.17330.a.B2.PS02120
Publications by SSGCID
Evolutionary Diversification of Host-Targeted Bartonella Effectors Proteins Derived from a Conserved FicTA Toxin-Antitoxin Module
de Beer T, Dehio C, Edwards T, Harms A, Myler P, Phan I, Schirmer T, Dranow D, Tamegger S, Dietz N
Microorganisms - 2021
volume 9, issue 8, pages 1645
PMID: 34442725; PMCID: PMC8401265
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|382640.5.peg.1760 |
| RefSeq: | YP_001610011.1 |
| UniProt: | A9IWP7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLEQNYLYKG TTTLKNKYGI KDPNKLYERC NHDVVKEAVN FRHEPPPQNF DAAYLSLIHW SLFHKTFEWA GHTRDTSFTF EDGTTARMPA MRPKGYEAPF AIGPQIKKEL KQLEKTLNQK NNLKGLSHQE FAENAADVFM ALEHAHPFRK GNGRANRMFM EKLGQAAGHT VDFSFITKGR MTTACIEAMQ YGNSQPMKDL FEDITHPQKS VILKEFISQM REAGLNEINN RVVVAAKEGI PYEGIFRGTS AEGFVMEIEG AFVVGHKDDL SPEQIKTLRN GDHLCFQKTN VQNVKETLIP KETLSPLTHE ALFAKISNDA YVEACRKEIE HLSKTVYGKM QALKTKLDIV TAAPNLGNQF ADEILENPQS ISKFAGRKIL GMKSSNRKYA EQTVPQLCQA LKNYGTITQQ AKDTILEQHQ REQNRLSHSV EKPKKNLQNL FSLSEEQQRE ALLHSPALQQ ELHTFSRQLK NRLSSEEHKA IHEKDHTRLA CLLGTSESKA KEIAHTVKNT KEAQCQARSL KVSRSSSLAL TG
NT Sequence
atgttagagc aaaattatct atataaagga accacaacac tcaaaaataa atatggcata aaagacccta ataaactgta tgaacgatgt aaccatgacg ttgttaaaga agccgtcaac tttcgccatg aaccacctcc acaaaacttt gatgccgctt atctaagctt aatccactgg agtctcttcc acaaaacttt tgaatgggca ggacacaccc gcgatacatc ctttacattt gaagatggca ccactgcccg catgccagcg atgcgtccta aaggttatga agctcctttt gcgatcggtc cacagataaa aaaagaactc aaacaacttg aaaaaacact gaatcagaag aacaatttaa aaggtttatc gcaccaagaa tttgctgaaa atgccgctga cgtttttatg gcactcgagc acgcacaccc ttttagaaaa ggcaatgggc gcgccaatcg aatgtttatg gaaaaactgg gacaagccgc aggacatacc gttgactttt ctttcatcac aaaaggacgc atgacaacag cttgtattga agccatgcaa tatggcaatt cccaaccgat gaaagatctt tttgaagata tcactcatcc acaaaaatcc gttattttaa aagaattcat ctcccagatg agagaagctg gactgaatga aattaacaat cgtgttgttg ttgctgcaaa ggaaggaatc ccctatgaag gcatctttag aggcacttca gcggaagggt ttgttatgga aatagaggga gctttcgtcg ttgggcacaa agatgatctc tccccagaac agatcaaaac attacgcaat ggcgaccacc tctgctttca aaaaaccaat gttcaaaatg taaaagaaac actgatccca aaagaaacat tatcccctct cacccatgaa gcgttattcg caaagatttc aaatgacgct tatgtagaag catgccgaaa agaaatcgaa catttatcaa agactgttta tggtaaaatg caagccttaa agacaaaatt ggatatcgta acagccgctc caaacttagg aaatcagttt gccgatgaaa ttttagaaaa ccctcaatca atttctaaat ttgctgggcg taaaatactt ggcatgaaaa gctcaaatcg caagtacgca gaacaaactg ttccacaact ctgccaagcc cttaaaaatt atggtacaat aacacaacaa gcaaaagata caattctaga acaacaccaa agagaacaaa atcgcctaag ccattcagtc gaaaaaccta aaaaaaattt acaaaacctt ttttccttat cagaagaaca acaaagggaa gctttattgc attctccagc actacaacaa gaactccata ccttttcacg tcaactaaaa aatcgtctct cttcagaaga gcataaagcc atacacgaaa aagatcatac aagactggcg tgccttcttg gtacatcaga aagcaaagca aaagaaattg cacacactgt aaagaatacc aaagaagcac aatgtcaagc ccgcagctta aaagttagtc gttcctcatc actggctctt acaggctaa
Details for BatrA.17330.a.B1.GE35950
| HARVESTED ON: | 8/28/2012 |
| SEQUENCED ON: | 2/22/2016 |
| EXPECTED MW: | 61kDa |
| OBSERVED MW: | 64kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MLEQNYLYKG TTTLKNKYGI KDPNKLYERC NHDVVKEAVN FRHEPPPQNF DAAYLSLIHW SLFHKTFEWA GHTRDTSFTF EDGTTARMPA MRPKGYEAPF AIGPQIKKEL KQLEKTLNQK NNLKGLSHQE FAENAADVFM ALEHAHPFRK GNGRANRMFM EKLGQAAGHT VDFSFITKGR MTTACIEAMQ YGNSQPMKDL FEDITHPQKS VILKEFISQM REAGLNEINN RVVVAAKEGI PYEGIFRGTS AEGFVMEIEG AFVVGHKDDL SPEQIKTLRN GDHLCFQKTN VQNVKETLIP KETLSPLTHE ALFAKISNDA YVEACRKEIE HLSKTVYGKM QALKTKLDIV TAAPNLGXQF ADEILENPQS ISKFAGRKIL GMKSSNRKYA EQTVPQLCQA LKNYGTITQQ AKDTILEQHQ REQNRLSHSV EKPKKNLQNL FSLSEEQQRE ALLHSPALQQ ELHTFSRQLK NRLSSEEHKA IHEKDHTRLA CLLGTSESKA KEIAHTVKNT KEAQCQARSL
Validated NT Sequence
ccaccaccat atgttagagc aaaattatct atataaagga accacaacac tcaaaaataa atatggcata aaagacccta ataaactgta tgaacgatgt aaccatgacg ttgttaaaga agccgtcaac tttcgccatg aaccacctcc acaaaacttt gatgccgctt atctaagctt aatccactgg agtctcttcc acaaaacttt tgaatgggca ggacacaccc gcgatacatc ctttacattt gaagatggca ccactgcccg catgccagcg atgcgtccta aaggttatga agctcctttt gcgatcggtc cacagataaa aaaagaactc aaacaacttg aaaaaacact gaatcagaag aacaatttaa aaggtttatc gcaccaagaa tttgctgaaa atgccgctga cgtttttatg gcactcgagc acgcacaccc ttttagaaaa ggcaatgggc gcgccaatcg aatgtttatg gaaaaactgg gacaagccgc aggacatacc gttgactttt ctttcatcac aaaaggacgc atgacaacag cttgtattga agccatgcaa tatggcaatt cccaaccgat gaaagatctt tttgaagata tcactcatcc acaaaaatcc gttattttaa aagaattcat ctcccagatg agagaagctg gactgaatga aattaacaat cgtgttgttg ttgctgcaaa ggaaggaatc ccctatgaag gcatctttag aggcacttca gcggaagggt ttgttatgga aatagaggga gctttcgtcg ttgggcacaa agatgatctc tccccagaac agatcaaaac attacgcaat ggcgaccacc tctgctttca aaaaaccaat gttcaaaatg taaaagaaac actgatccca aaagaaacat tatcccctct cacccatgaa gcgttattcg caaagatttc aaatgacgct tatgtagaag catgccgaaa agaaatcgaa catttatcaa agactgttta tggtaaaatg caagccttaa agacaaaatt ggatatcgta acagccgctc caaacttagg aaancagttt gccgatgaaa ttttagaaaa ccctcaatca atttctaaat ttgctgggcg taaaatactt ggcatgaaaa gctcaaatcg caagtacgca gaacaaactg ttccacaact ctgccaagcc cttaaaaatt atggtacaat aacacaacaa gcaaaagata caattctaga acaacaccaa agagaacaaa atcgcctaag ccattcagtc gaaaaaccta aaaaaaattt acaaaacctt ttttccttat cagaagaaca acaaagggaa gctttattgc attctccagc actacaacaa gaactccata ccttttcacg tcaactaaaa aatcgtctct cttcagaaga gcataaagcc atacacgaaa aagatcatac aagactggcg tgccttcttg gtacatcaga aagcaaagca aaagaaattg cacacactgt aaagaatacc aaagaagcac aatgtcaagc ccgcagctta aa
Expected Protein Sequence
MAHHHHHHML EQNYLYKGTT TLKNKYGIKD PNKLYERCNH DVVKEAVNFR HEPPPQNFDA AYLSLIHWSL FHKTFEWAGH TRDTSFTFED GTTARMPAMR PKGYEAPFAI GPQIKKELKQ LEKTLNQKNN LKGLSHQEFA ENAADVFMAL EHAHPFRKGN GRANRMFMEK LGQAAGHTVD FSFITKGRMT TACIEAMQYG NSQPMKDLFE DITHPQKSVI LKEFISQMRE AGLNEINNRV VVAAKEGIPY EGIFRGTSAE GFVMEIEGAF VVGHKDDLSP EQIKTLRNGD HLCFQKTNVQ NVKETLIPKE TLSPLTHEAL FAKISNDAYV EACRKEIEHL SKTVYGKMQA LKTKLDIVTA APNLGNQFAD EILENPQSIS KFAGRKILGM KSSNRKYAEQ TVPQLCQALK NYGTITQQAK DTILEQHQRE QNRLSHSVEK PKKNLQNLFS LSEEQQREAL LHSPALQQEL HTFSRQLKNR LSSEEHKAIH EKDHTRLACL LGTSESKAKE IAHTVKNTKE AQCQARSLKV SRSSSLALTG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttag agcaaaatta tctatataaa ggaaccacaa cactcaaaaa taaatatggc ataaaagacc ctaataaact gtatgaacga tgtaaccatg acgttgttaa agaagccgtc aactttcgcc atgaaccacc tccacaaaac tttgatgccg cttatctaag cttaatccac tggagtctct tccacaaaac ttttgaatgg gcaggacaca cccgcgatac atcctttaca tttgaagatg gcaccactgc ccgcatgcca gcgatgcgtc ctaaaggtta tgaagctcct tttgcgatcg gtccacagat aaaaaaagaa ctcaaacaac ttgaaaaaac actgaatcag aagaacaatt taaaaggttt atcgcaccaa gaatttgctg aaaatgccgc tgacgttttt atggcactcg agcacgcaca cccttttaga aaaggcaatg ggcgcgccaa tcgaatgttt atggaaaaac tgggacaagc cgcaggacat accgttgact tttctttcat cacaaaagga cgcatgacaa cagcttgtat tgaagccatg caatatggca attcccaacc gatgaaagat ctttttgaag atatcactca tccacaaaaa tccgttattt taaaagaatt catctcccag atgagagaag ctggactgaa tgaaattaac aatcgtgttg ttgttgctgc aaaggaagga atcccctatg aaggcatctt tagaggcact tcagcggaag ggtttgttat ggaaatagag ggagctttcg tcgttgggca caaagatgat ctctccccag aacagatcaa aacattacgc aatggcgacc acctctgctt tcaaaaaacc aatgttcaaa atgtaaaaga aacactgatc ccaaaagaaa cattatcccc tctcacccat gaagcgttat tcgcaaagat ttcaaatgac gcttatgtag aagcatgccg aaaagaaatc gaacatttat caaagactgt ttatggtaaa atgcaagcct taaagacaaa attggatatc gtaacagccg ctccaaactt aggaaatcag tttgccgatg aaattttaga aaaccctcaa tcaatttcta aatttgctgg gcgtaaaata cttggcatga aaagctcaaa tcgcaagtac gcagaacaaa ctgttccaca actctgccaa gcccttaaaa attatggtac aataacacaa caagcaaaag atacaattct agaacaacac caaagagaac aaaatcgcct aagccattca gtcgaaaaac ctaaaaaaaa tttacaaaac cttttttcct tatcagaaga acaacaaagg gaagctttat tgcattctcc agcactacaa caagaactcc ataccttttc acgtcaacta aaaaatcgtc tctcttcaga agagcataaa gccatacacg aaaaagatca tacaagactg gcgtgccttc ttggtacatc agaaagcaaa gcaaaagaaa ttgcacacac tgtaaagaat accaaagaag cacaatgtca agcccgcagc ttaaaagtta gtcgttcctc atcactggct cttacaggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BatrA.17330.a.B3.GE37279
| HARVESTED ON: | 8/28/2013 |
| SEQUENCED ON: | 9/3/2013 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHME QNYLYKGTTT LKNKYGIKDX NKLYERCNHD VVKEAVNFRH EPPPQNFDAA YLSLIHXSLF HKTFEWAGHT RDTSFTFEDG TTARMPAMRP KGYEAPFAIG PQIKKELKQL EKTLNQKNNL KGLSHQEFAE NAADVFMALE HAHPFRKGNG RANRMFMEKL GQAAGHTVDF SFITKGRMTT ACIEAMQYGN SQPMKDLFED ITHPQKSVIL KEFISQMREA GLNEINNRVV VAAKEGIPYE GIFRGTSAEG FVMEIEGAFV VGHKDDLSPE QIKTLRNGDH LCFQ
Validated NT Sequence
atcttactca cttattgaaa gcagaggtgg tcgccattgc gtaatgtttt gatctgttct ggggagagat catctttgtg cccaacgacg aaagctccct ctatttccat aacaaaccct tccgctgaag tgcctctaaa gatgccttca taggggattc cttcctttgc agcaacaaca acacgattgt taatttcatt cagtccagct tctctcatct gggagatgaa ttcttttaaa ataacggatt tttgtggatg agtgatatct tcaaaaagat ctttcatcgg ttgggaattg ccatattgca tggcttcaat acaagctgtt gtcatgcgtc cttttgtgat gaaagaaaag tcaacggtat gtcctgcggc ttgtcccagt ttttccataa acattcgatt ggcgcgccca ttgccttttc taaaagggtg tgcgtgctcg agtgccataa aaacgtcagc ggcattttca gcaaattctt ggtgcgataa accttttaaa ttgttcttct gattcagtgt tttttcaagt tgtttgagtt ctttttttat ctgtggaccg atcgcaaaag gagcttcata acctttagga cgcatcgctg gcatgcgggc agtggtgcca tcttcaaatg taaaggatgt atcgcgggtg tgtcctgccc attcaaaagt tttgtggaag agactccngt ggattaagct tagataagcg gcatcaaagt tttgtggagg tggttcatgg cgaaagttga cggcttcttt aacaacgtca tggttacatc gttcatacag tttattangg tcttttatgc catatttatt tttgagtgtt gtggttcctt tatatagata attttgctcc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHEQ NYLYKGTTTL KNKYGIKDPN KLYERCNHDV VKEAVNFRHE PPPQNFDAAY LSLIHWSLFH KTFEWAGHTR DTSFTFEDGT TARMPAMRPK GYEAPFAIGP QIKKELKQLE KTLNQKNNLK GLSHQEFAEN AADVFMALEH AHPFRKGNGR ANRMFMEKLG QAAGHTVDFS FITKGRMTTA CIEAMQYGNS QPMKDLFEDI THPQKSVILK EFISQMREAG LNEINNRVVV AAKEGIPYEG IFRGTSAEGF VMEIEGAFVV GHKDDLSPEQ IKTLRNGDHL CFQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgagcaaa attatctata taaaggaacc acaacactca aaaataaata tggcataaaa gaccctaata aactgtatga acgatgtaac catgacgttg ttaaagaagc cgtcaacttt cgccatgaac cacctccaca aaactttgat gccgcttatc taagcttaat ccactggagt ctcttccaca aaacttttga atgggcagga cacacccgcg atacatcctt tacatttgaa gatggcacca ctgcccgcat gccagcgatg cgtcctaaag gttatgaagc tccttttgcg atcggtccac agataaaaaa agaactcaaa caacttgaaa aaacactgaa tcagaagaac aatttaaaag gtttatcgca ccaagaattt gctgaaaatg ccgctgacgt ttttatggca ctcgagcacg cacacccttt tagaaaaggc aatgggcgcg ccaatcgaat gtttatggaa aaactgggac aagccgcagg acataccgtt gacttttctt tcatcacaaa aggacgcatg acaacagctt gtattgaagc catgcaatat ggcaattccc aaccgatgaa agatcttttt gaagatatca ctcatccaca aaaatccgtt attttaaaag aattcatctc ccagatgaga gaagctggac tgaatgaaat taacaatcgt gttgttgttg ctgcaaagga aggaatcccc tatgaaggca tctttagagg cacttcagcg gaagggtttg ttatggaaat agagggagct ttcgtcgttg ggcacaaaga tgatctctcc ccagaacaga tcaaaacatt acgcaatggc gaccacctct gctttcaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BatrA.17330.a.B2.GE37278
| HARVESTED ON: | 8/28/2013 |
| SEQUENCED ON: | 9/3/2013 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHME QNYLYKGTTT LKNKYGIKDP NKLYERCNHD VVKEAVNFRH EPPPQNFDAA YLSLIHWSLF HKTFEWAGHT RDTSFTFEDG TTARMPAMRP KGYEAPFAIG PQIKKELKQL EKTLNQKNNL KGLSHQEFAE NAADVFMALE HAHPFRKGNG RANRMFMEKL GQAAGHTVDF SFITKGRMTT ACIEAMQYGN SQPMKDLFED ITHPQKSVIL KEFISQM
Validated NT Sequence
atcttactca cttacatctg ggagatgaat tcttttaaaa taacggattt ttgtggatga gtgatatctt caaaaagatc tttcatcggt tgggaattgc catattgcat ggcttcaata caagctgttg tcatgcgtcc ttttgtgatg aaagaaaagt caacggtatg tcctgcggct tgtcccagtt tttccataaa cattcgattg gcgcgcccat tgccttttct aaaagggtgt gcgtgctcga gtgccataaa aacgtcagcg gcattttcag caaattcttg gtgcgataaa ccttttaaat tgttcttctg attcagtgtt ttttcaagtt gtttgagttc tttttttatc tgtggaccga tcgcaaaagg agcttcataa cctttaggac gcatcgctgg catgcgggca gtggtgccat cttcaaatgt aaaggatgta tcgcgggtgt gtcctgccca ttcaaaagtt ttgtggaaga gactccagtg gattaagctt agataagcgg catcaaagtt ttgtggaggt ggttcatggc gaaagttgac ggcttcttta acaacgtcat ggttacatcg ttcatacagt ttattagggt cttttatgcc atatttattt ttgagtgttg tggttccttt atatagataa ttttgctcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHEQ NYLYKGTTTL KNKYGIKDPN KLYERCNHDV VKEAVNFRHE PPPQNFDAAY LSLIHWSLFH KTFEWAGHTR DTSFTFEDGT TARMPAMRPK GYEAPFAIGP QIKKELKQLE KTLNQKNNLK GLSHQEFAEN AADVFMALEH AHPFRKGNGR ANRMFMEKLG QAAGHTVDFS FITKGRMTTA CIEAMQYGNS QPMKDLFEDI THPQKSVILK EFISQM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgagcaaa attatctata taaaggaacc acaacactca aaaataaata tggcataaaa gaccctaata aactgtatga acgatgtaac catgacgttg ttaaagaagc cgtcaacttt cgccatgaac cacctccaca aaactttgat gccgcttatc taagcttaat ccactggagt ctcttccaca aaacttttga atgggcagga cacacccgcg atacatcctt tacatttgaa gatggcacca ctgcccgcat gccagcgatg cgtcctaaag gttatgaagc tccttttgcg atcggtccac agataaaaaa agaactcaaa caacttgaaa aaacactgaa tcagaagaac aatttaaaag gtttatcgca ccaagaattt gctgaaaatg ccgctgacgt ttttatggca ctcgagcacg cacacccttt tagaaaaggc aatgggcgcg ccaatcgaat gtttatggaa aaactgggac aagccgcagg acataccgtt gacttttctt tcatcacaaa aggacgcatg acaacagctt gtattgaagc catgcaatat ggcaattccc aaccgatgaa agatcttttt gaagatatca ctcatccaca aaaatccgtt attttaaaag aattcatctc ccagatgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BatrA.17330.a.B4.GE37280
| HARVESTED ON: | 8/28/2013 |
| SEQUENCED ON: | 9/3/2013 |
| EXPECTED MW: | 16kDa |
| OBSERVED MW: | 16kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHML IPKETLSPLT HEALFAKISN DAYVEACRKE IEHLSKTVYG KMQALKTKLD IVTAAPNLGN QFADEILENP QSISKFAGRK ILGMKSSNRK YAEQTVPQLC QALKNYGTIT QQAKDTILEQ HQREQNRLSH SVEKPK
Validated NT Sequence
atcttactca cttatttagg tttttcgact gaatggctta ggcgattttg ttctctttgg tgttgttcta gaattgtatc ttttgcttgt tgtgttattg taccataatt tttaagggct tggcagagtt gtggaacagt ttgttctgcg tacttgcgat ttgagctttt catgccaagt attttacgcc cagcaaattt agaaattgat tgagggtttt ctaaaatttc atcggcaaac tgatttccta agtttggagc ggctgttacg atatccaatt ttgtctttaa ggcttgcatt ttaccataaa cagtctttga taaatgttcg atttcttttc ggcatgcttc tacataagcg tcatttgaaa tctttgcgaa taacgcttca tgggtgagag gggataatgt ttcttttggg atcagcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHLI PKETLSPLTH EALFAKISND AYVEACRKEI EHLSKTVYGK MQALKTKLDI VTAAPNLGNQ FADEILENPQ SISKFAGRKI LGMKSSNRKY AEQTVPQLCQ ALKNYGTITQ QAKDTILEQH QREQNRLSHS VEKPK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctgatcc caaaagaaac attatcccct ctcacccatg aagcgttatt cgcaaagatt tcaaatgacg cttatgtaga agcatgccga aaagaaatcg aacatttatc aaagactgtt tatggtaaaa tgcaagcctt aaagacaaaa ttggatatcg taacagccgc tccaaactta ggaaatcagt ttgccgatga aattttagaa aaccctcaat caatttctaa atttgctggg cgtaaaatac ttggcatgaa aagctcaaat cgcaagtacg cagaacaaac tgttccacaa ctctgccaag cccttaaaaa ttatggtaca ataacacaac aagcaaaaga tacaattcta gaacaacacc aaagagaaca aaatcgccta agccattcag tcgaaaaacc taaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BatrA.17330.a.B2.PS02120
| PURIFICATION DATe: | 7/29/2014 |
| CONCENTRATION: | 22.75mg/ml |
| OBSERVED MW: | 26kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME QNYLYKGTTT LKNKYGIKDP NKLYERCNHD VVKEAVNFRH EPPPQNFDAA YLSLIHWSLF HKTFEWAGHT RDTSFTFEDG TTARMPAMRP KGYEAPFAIG PQIKKELKQL EKTLNQKNNL KGLSHQEFAE NAADVFMALE HAHPFRKGNG RANRMFMEKL GQAAGHTVDF SFITKGRMTT ACIEAMQYGN SQPMKDLFED ITHPQKSVIL KEFISQM
Validated NT Sequence
atcttactca cttacatctg ggagatgaat tcttttaaaa taacggattt ttgtggatga gtgatatctt caaaaagatc tttcatcggt tgggaattgc catattgcat ggcttcaata caagctgttg tcatgcgtcc ttttgtgatg aaagaaaagt caacggtatg tcctgcggct tgtcccagtt tttccataaa cattcgattg gcgcgcccat tgccttttct aaaagggtgt gcgtgctcga gtgccataaa aacgtcagcg gcattttcag caaattcttg gtgcgataaa ccttttaaat tgttcttctg attcagtgtt ttttcaagtt gtttgagttc tttttttatc tgtggaccga tcgcaaaagg agcttcataa cctttaggac gcatcgctgg catgcgggca gtggtgccat cttcaaatgt aaaggatgta tcgcgggtgt gtcctgccca ttcaaaagtt ttgtggaaga gactccagtg gattaagctt agataagcgg catcaaagtt ttgtggaggt ggttcatggc gaaagttgac ggcttcttta acaacgtcat ggttacatcg ttcatacagt ttattagggt cttttatgcc atatttattt ttgagtgttg tggttccttt atatagataa ttttgctcca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHEQ NYLYKGTTTL KNKYGIKDPN KLYERCNHDV VKEAVNFRHE PPPQNFDAAY LSLIHWSLFH KTFEWAGHTR DTSFTFEDGT TARMPAMRPK GYEAPFAIGP QIKKELKQLE KTLNQKNNLK GLSHQEFAEN AADVFMALEH AHPFRKGNGR ANRMFMEKLG QAAGHTVDFS FITKGRMTTA CIEAMQYGNS QPMKDLFEDI THPQKSVILK EFISQM
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGAGCAAA ATTATCTATA TAAAGGAACC ACAACACTCA AAAATAAATA TGGCATAAAA GACCCTAATA AACTGTATGA ACGATGTAAC CATGACGTTG TTAAAGAAGC CGTCAACTTT CGCCATGAAC CACCTCCACA AAACTTTGAT GCCGCTTATC TAAGCTTAAT CCACTGGAGT CTCTTCCACA AAACTTTTGA ATGGGCAGGA CACACCCGCG ATACATCCTT TACATTTGAA GATGGCACCA CTGCCCGCAT GCCAGCGATG CGTCCTAAAG GTTATGAAGC TCCTTTTGCG ATCGGTCCAC AGATAAAAAA AGAACTCAAA CAACTTGAAA AAACACTGAA TCAGAAGAAC AATTTAAAAG GTTTATCGCA CCAAGAATTT GCTGAAAATG CCGCTGACGT TTTTATGGCA CTCGAGCACG CACACCCTTT TAGAAAAGGC AATGGGCGCG CCAATCGAAT GTTTATGGAA AAACTGGGAC AAGCCGCAGG ACATACCGTT GACTTTTCTT TCATCACAAA AGGACGCATG ACAACAGCTT GTATTGAAGC CATGCAATAT GGCAATTCCC AACCGATGAA AGATCTTTTT GAAGATATCA CTCATCCACA AAAATCCGTT ATTTTAAAAG AATTCATCTC CCAGATGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for BatrA.17330.a.B4.PS01976
| PURIFICATION DATe: | 1/21/2014 |
| CONCENTRATION: | 21mg/ml |
| OBSERVED MW: | 16kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML IPKETLSPLT HEALFAKISN DAYVEACRKE IEHLSKTVYG KMQALKTKLD IVTAAPNLGN QFADEILENP QSISKFAGRK ILGMKSSNRK YAEQTVPQLC QALKNYGTIT QQAKDTILEQ HQREQNRLSH SVEKPK
Validated NT Sequence
atcttactca cttatttagg tttttcgact gaatggctta ggcgattttg ttctctttgg tgttgttcta gaattgtatc ttttgcttgt tgtgttattg taccataatt tttaagggct tggcagagtt gtggaacagt ttgttctgcg tacttgcgat ttgagctttt catgccaagt attttacgcc cagcaaattt agaaattgat tgagggtttt ctaaaatttc atcggcaaac tgatttccta agtttggagc ggctgttacg atatccaatt ttgtctttaa ggcttgcatt ttaccataaa cagtctttga taaatgttcg atttcttttc ggcatgcttc tacataagcg tcatttgaaa tctttgcgaa taacgcttca tgggtgagag gggataatgt ttcttttggg atcagcatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHLI PKETLSPLTH EALFAKISND AYVEACRKEI EHLSKTVYGK MQALKTKLDI VTAAPNLGNQ FADEILENPQ SISKFAGRKI LGMKSSNRKY AEQTVPQLCQ ALKNYGTITQ QAKDTILEQH QREQNRLSHS VEKPK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTGATCC CAAAAGAAAC ATTATCCCCT CTCACCCATG AAGCGTTATT CGCAAAGATT TCAAATGACG CTTATGTAGA AGCATGCCGA AAAGAAATCG AACATTTATC AAAGACTGTT TATGGTAAAA TGCAAGCCTT AAAGACAAAA TTGGATATCG TAACAGCCGC TCCAAACTTA GGAAATCAGT TTGCCGATGA AATTTTAGAA AACCCTCAAT CAATTTCTAA ATTTGCTGGG CGTAAAATAC TTGGCATGAA AAGCTCAAAT CGCAAGTACG CAGAACAAAC TGTTCCACAA CTCTGCCAAG CCCTTAAAAA TTATGGTACA ATAACACAAC AAGCAAAAGA TACAATTCTA GAACAACACC AAAGAGAACA AAATCGCCTA AGCCATTCAG TCGAAAAACC TAAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT