SSGCID: Target Page

BatrA.01664.a

Two-component sensor kinase BatS (EC 2.7.3.-) (BatR-BatS two-component system)

CENTER ID:	BatrA.01664.a
ORGANISM:	Bartonella tribocorum CIP 105476 / IBS 506
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BatrA.01664.a.B2.GE35948	kinase domain		333	594
BatrA.01664.a.B3.GE37277	periplasmic domain		68	279

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BatrA.01664.a.B2.PS01684	kinase domain		333	594

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|382640.5.peg.83
RefSeq:	YP_001608557.1
UniProt:	A9ILG5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDNSQLETL KKTAPNGVSS TPFLMFARLR LRTQRLFRQL LFSSLTRRIV ILNIAALAVL VTGILYLNQF RDGLIEAKIK SLCTQGKIIA GAIAASATVD TNSILIDPQK LLELQTGESV TPAPQSTDSW DFPINPEQVA PLLRRLITPK TTRARIYDRD ATLLLDSRVL YSSGEVFSYD LPPLKTEQNI WDRLTAWYSN TFYDKGIALD REQTKNRGIA YPEVYRALNG SLATAKRRNR QGQLIVSVAV PVQRYRAVVG ALLLSTTGSD IDNIVKAERL VIFKVFAVVG SVLLVLSLFL AHTIAHPLSK LSASANRVRN GNNQRVEIPD FSMREDEIGH LSTSIRDMTN ALYMRIEAIE RFAADVSHEL KNPLTSLRSA VETLPLAKNE EAQEKLLEII QHDVRRLDRL ITDISDASRL DAELARETAE IVDMKPLLES LVHAVQEVYR NAQTIDVSLN IVPRPHGKAY LVLGHELRLG QVISNLLENA RSFIPRNRGK ISITMKSHAS TLILTIEDNG PGIRSENIER IFERFYTDRP NEDAFGQNSG LGLSISRQII EAHNGTITAE NIIDPALGKC KTGARFIIML PLVK

NT Sequence

atgacagaca attctcaact ggaaactcta aaaaaaacag cccctaacgg tgtatcttca acgccatttc ttatgtttgc tcgtttgcgt cttcgaacac aacgtctctt tagacaattg cttttttcca gtctcacacg ccgcattgtt atattaaaca ttgctgccct tgccgttctg gttacaggca ttttatacct taaccaattt cgtgatggtt taattgaagc aaaaattaaa agtttatgta cccaaggtaa aattatcgct ggagctattg ctgcttctgc aacggtagat acaaattcta tccttattga cccccaaaaa ctcttggagt tacagaccgg tgaaagcgtt acacccgccc ctcaatcaac cgactcttgg gattttccca ttaaccctga gcaagttgcc cctcttttac gacgtctcat cacacctaaa acgacaagag cacgtatcta tgatcgtgat gctactttac ttcttgattc gcgcgttctt tattctagtg gagaagtttt tagctatgat ttacccccac ttaaaacaga acaaaacata tgggatcgct tgactgcatg gtattcaaac acattttatg acaaaggtat tgctcttgat agagagcaaa caaaaaaccg tggcattgct tatccagaag tgtaccgagc attaaacgga tctcttgcta ccgccaaaag acgcaacaga caaggtcaac tcattgtttc tgttgccgtt cctgttcaac gctatcgcgc agtggttggc gcccttcttc tttcaaccac tggctctgat attgataaca ttgtaaaagc tgaaagactg gttatattta aagtttttgc cgttgtaggc agcgtccttt tggttctttc gctcttttta gcccatacaa ttgcccatcc cttaagtaaa ttatctgcct ctgctaaccg agtacgtaat ggcaataatc agcgtgtaga aattcctgat ttttcaatgc gtgaagatga aattggccat ctctcaacct ctattcgtga catgaccaat gccctttata tgcgtattga agccattgaa cgctttgctg ctgatgtaag ccacgaatta aaaaatcccc tcacttctct acgcagtgct gttgaaaccc tccctctggc taaaaatgaa gaagcacaag aaaaattgct tgaaattatt caacacgatg tgcgccgtct tgatcggttg attacagata tttctgatgc atcgcgactt gatgcagagc ttgcacgaga aaccgcggag atcgttgata tgaaaccgct tttggaaagt cttgttcatg ccgttcaaga agtataccgc aatgcgcaaa ccatcgatgt aagccttaat attgtcccac gtcctcatgg aaaagcttat cttgtgttgg gacatgaact ccgtttaggg caagtcattt ccaatcttct tgaaaatgca cgttccttta ttccccgtaa tcgtggcaaa atttccatta ctatgaaaag tcatgcttca acccttattt taaccattga agacaatggc cccggtattc gttcagaaaa tattgaacgt atttttgaac gtttttatac agatcgacca aatgaagatg cttttggaca aaactctgga cttgggcttt ctattagtcg gcaaatcatc gaagcccata atgggacaat tacagccgaa aatattattg atccggcact tggaaaatgt aaaactggtg cacgttttat tattatgctt cctctcgtta aataa

Details for BatrA.01664.a.B2.GE35948

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR EDEIGHLSTS IRDMTNALYM RIEAIERFAA DVSHELKNPL TSLRSAVETL PLAKNEEAQE KLLEIIQHDV RRLDRLITDI SDASRLDAEL ARETAEIVDM KPLLESLVHA VQEVYRNAQT IDVSLNIVPR PHGKAYLVLG HELRLGQVIS NLLENARSFI PRNRGKISIT MKSHASTLIL TIEDNGPGIR SENIERIFER FYTDRPNEDA FGQNSGLGLS ISRQIIEAHN GTITAENIID PALGKCKTGA RFIIMLPLVK

Validated NT Sequence

cagccggntc ctatcttact cacttattta acgagaggaa gcataataat aaaacgtgca ccagttttac attttccaag tgccggatca ataatatttt cggctgtaat tgtcccatta tgggcttcga tgatttgccg actaatagaa agcccaagtc cagagttttg tccaaaagca tcttcatttg gtcgatctgt ataaaaacgt tcaaaaatac gttcaatatt ttctgaacga ataccggggc cattgtcttc aatggttaaa ataagggttg aagcatgact tttcatagta atggaaattt tgccacgatt acggggaata aaggaacgtg cattttcaag aagattggaa atgacttgcc ctaaacggag ttcatgtccc aacacaagat aagcttttcc atgaggacgt gggacaatat taaggcttac atcgatggtt tgcgcattgc ggtatacttc ttgaacggca tgaacaagac tttccaaaag cggtttcata tcaacgatct ccgcggtttc tcgtgcaagc tctgcatcaa gtcgcgatgc atcagaaata tctgtaatca accgatcaag acggcgcaca tcgtgttgaa taatttcaag caatttttct tgtgcttctt catttttagc cagagggagg gtttcaacag cactgcgtag agaagtgagg ggatttttta attcgtggct tacatcagca gcaaagcgtt caatggcttc aatacgcata taaagggcat tggtcatgtc acgaatagag gttgagagat ggccaatttc atcttcacgc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg aagatgaaat tggccatctc tcaacctcta ttcgtgacat gaccaatgcc ctttatatgc gtattgaagc cattgaacgc tttgctgctg atgtaagcca cgaattaaaa aatcccctca cttctctacg cagtgctgtt gaaaccctcc ctctggctaa aaatgaagaa gcacaagaaa aattgcttga aattattcaa cacgatgtgc gccgtcttga tcggttgatt acagatattt ctgatgcatc gcgacttgat gcagagcttg cacgagaaac cgcggagatc gttgatatga aaccgctttt ggaaagtctt gttcatgccg ttcaagaagt ataccgcaat gcgcaaacca tcgatgtaag ccttaatatt gtcccacgtc ctcatggaaa agcttatctt gtgttgggac atgaactccg tttagggcaa gtcatttcca atcttcttga aaatgcacgt tcctttattc cccgtaatcg tggcaaaatt tccattacta tgaaaagtca tgcttcaacc cttattttaa ccattgaaga caatggcccc ggtattcgtt cagaaaatat tgaacgtatt tttgaacgtt tttatacaga tcgaccaaat gaagatgctt ttggacaaaa ctctggactt gggctttcta ttagtcggca aatcatcgaa gcccataatg ggacaattac agccgaaaat attattgatc cggcacttgg aaaatgtaaa actggtgcac gttttattat tatgcttcct ctcgttaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BatrA.01664.a.B3.GE37277

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN QFRDGLIEAK IKSLCTQGKI IAGAIAASAT VDTNSILIDP QKLLELQTGE SVTPAPQSTD SWDFPINPEQ VAPLLRRLIT PKTTRARIYD RDATLLLDSR VLYSSGEVFS YDLPPLKTEQ NIWDRLTAWY SNTFYDKGIA LDREQTKNRG IAYPEVYRAL NGSLATAKRR NRQGQLIVSV AVPVQRYRAV VGALLLSTTG SDIDNIVKAE R

Validated NT Sequence

atcttactca cttatctttc agcttttaca atgttatcaa tatcagagcc agtggttgaa agaagaaggg cgccaaccac tgcgcgatag cgttgaacag gaacggcaac agaaacaatg agttgacctt gtctgttgcg tcttttggcg gtagcaagag atccgtttaa tgctcggtac acttctggat aagcaatgcc acggtttttt gtttgctctc tatcaagagc aatacctttg tcataaaatg tgtttgaata ccatgcagtc aagcgatccc atatgttttg ttctgtttta agtgggggta aatcatagct aaaaacttct ccactagaat aaagaacgcg cgaatcaaga agtaaagtag catcacgatc atagatacgt gctcttgtcg ttttaggtgt gatgagacgt cgtaaaagag gggcaacttg ctcagggtta atgggaaaat cccaagagtc ggttgattga ggggcgggtg taacgctttc accggtctgt aactccaaga gtttttgggg gtcaataagg atagaatttg tatctaccgt tgcagaagca gcaatagctc cagcgataat tttaccttgg gtacataaac ttttaatttt tgcttcaatt aaaccatcac gaaattggtt catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHNQ FRDGLIEAKI KSLCTQGKII AGAIAASATV DTNSILIDPQ KLLELQTGES VTPAPQSTDS WDFPINPEQV APLLRRLITP KTTRARIYDR DATLLLDSRV LYSSGEVFSY DLPPLKTEQN IWDRLTAWYS NTFYDKGIAL DREQTKNRGI AYPEVYRALN GSLATAKRRN RQGQLIVSVA VPVQRYRAVV GALLLSTTGS DIDNIVKAER

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaccaat ttcgtgatgg tttaattgaa gcaaaaatta aaagtttatg tacccaaggt aaaattatcg ctggagctat tgctgcttct gcaacggtag atacaaattc tatccttatt gacccccaaa aactcttgga gttacagacc ggtgaaagcg ttacacccgc ccctcaatca accgactctt gggattttcc cattaaccct gagcaagttg cccctctttt acgacgtctc atcacaccta aaacgacaag agcacgtatc tatgatcgtg atgctacttt acttcttgat tcgcgcgttc tttattctag tggagaagtt tttagctatg atttaccccc acttaaaaca gaacaaaaca tatgggatcg cttgactgca tggtattcaa acacatttta tgacaaaggt attgctcttg atagagagca aacaaaaaac cgtggcattg cttatccaga agtgtaccga gcattaaacg gatctcttgc taccgccaaa agacgcaaca gacaaggtca actcattgtt tctgttgccg ttcctgttca acgctatcgc gcagtggttg gcgcccttct tctttcaacc actggctctg atattgataa cattgtaaaa gctgaaagat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BatrA.01664.a.B2.PS01684

PURIFICATION DATe:	11/16/2012
CONCENTRATION:	26.4mg/ml
OBSERVED MW:	31kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGTG AAGATGAAAT TGGCCATCTC TCAACCTCTA TTCGTGACAT GACCAATGCC CTTTATATGC GTATTGAAGC CATTGAACGC TTTGCTGCTG ATGTAAGCCA CGAATTAAAA AATCCCCTCA CTTCTCTACG CAGTGCTGTT GAAACCCTCC CTCTGGCTAA AAATGAAGAA GCACAAGAAA AATTGCTTGA AATTATTCAA CACGATGTGC GCCGTCTTGA TCGGTTGATT ACAGATATTT CTGATGCATC GCGACTTGAT GCAGAGCTTG CACGAGAAAC CGCGGAGATC GTTGATATGA AACCGCTTTT GGAAAGTCTT GTTCATGCCG TTCAAGAAGT ATACCGCAAT GCGCAAACCA TCGATGTAAG CCTTAATATT GTCCCACGTC CTCATGGAAA AGCTTATCTT GTGTTGGGAC ATGAACTCCG TTTAGGGCAA GTCATTTCCA ATCTTCTTGA AAATGCACGT TCCTTTATTC CCCGTAATCG TGGCAAAATT TCCATTACTA TGAAAAGTCA TGCTTCAACC CTTATTTTAA CCATTGAAGA CAATGGCCCC GGTATTCGTT CAGAAAATAT TGAACGTATT TTTGAACGTT TTTATACAGA TCGACCAAAT GAAGATGCTT TTGGACAAAA CTCTGGACTT GGGCTTTCTA TTAGTCGGCA AATCATCGAA GCCCATAATG GGACAATTAC AGCCGAAAAT ATTATTGATC CGGCACTTGG AAAATGTAAA ACTGGTGCAC GTTTTATTAT TATGCTTCCT CTCGTTAAAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT