BatrA.00720.a

VirB11 homologue (VirB type IV secretion machinery)

CENTER ID: BatrA.00720.a
ORGANISM: Bartonella tribocorum CIP 105476 / IBS 506
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BatrA.00720.a.B1.GE35945 full length 1 356
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BatrA.00720.a.B1.PS01696 full length 1 356

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|85701.4.peg.1799
RefSeq: WP_012232124.1
UniProt: Q8GJ57

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPNLRTMND EAVAIVLTKL EPISTFLRDE GLFEIVINRP YQVMTEGIDG WKTIEAPALS FNELMGIAKV VASYSKQNIS DRNPILSATL PGNERIQIVI PPAVEKDTIS MTIRKPSSQS FSLEELANKG LFSLCEQVSF TPLNDYHARF NELKIIEHNL ANAYCNKDFV SFLNQAVKCQ KNILIAGKTG SGKTTLSKAL IAKIPQDERI ITIEDTQELV IPHPNHVSMI YSKDGQGLAS VGPKELLESS LRMRPDRILL QELRDGTAFY YIRNVNSGHP GSITTVHAST ALAAFEQMTL LVKESEGGGD LERDDIRGLL ISMIDIIIQC KRVEGKFKVT EIYYDPFKQR NIFGGD
NT Sequence
atgaatccaa atttacgcac catgaatgat gaagcagtcg cgattgttct cacaaaactt gaacctatca gtactttttt gagagatgag ggtctttttg aaattgttat caatcgtccc tatcaagtga tgacggaagg aattgatggg tggaaaacga tagaagcacc tgctctctct tttaatgaac tgatgggaat tgcgaaagtt gtcgcgtcct attctaagca aaatatatca gataggaatc caatattatc agcgactctc ccaggcaatg agcgcattca aattgtcatt ccacccgctg tcgaaaaaga cacaattagt atgacaattc gtaaaccatc gtcgcagagt ttttctctcg aagagcttgc caataaaggt ctcttctcat tatgtgagca ggtctctttc acaccattga atgattatca tgcgcgtttt aacgaactca aaatcatcga gcataatcta gccaatgcct actgcaataa agactttgtt tcctttttaa atcaggctgt aaaatgccaa aaaaatattc tgattgcagg aaaaactggt tcgggtaaaa caacattatc aaaggcatta attgcaaaaa ttcctcaaga cgagcgtatt atcaccattg aagacacaca ggaattggtg ataccgcacc ctaaccatgt ctcgatgatc tattcaaaag atggacaagg cttagcttct gttggtccca aagaattact tgaatcatct ttacggatgc gtcctgatcg tattcttttg caagagcttc gagatggtac agccttttat tatatccgca atgtcaattc aggccaccca ggttccatta cgaccgttca tgcatcaaca gcccttgcag cctttgaaca aatgaccctt ttggtcaaag aaagtgaagg agggggggat ttagagcgtg atgatattcg aggtttattg atttcaatga ttgatattat catccaatgc aaacgggttg aaggaaaatt taaggtcaca gaaatttatt atgatccctt caagcaacgt aacatttttg gcggtgacta a
Details for BatrA.00720.a.B1.GE35945
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MNPNLRTMND EAVAIVLTKL EPISTFLRDE GLFEIVINRP YQVMTEGIDG WKTIEAPALS FNELMGIAKV VASYSKQNIS DRNPILSATL PGNERIQIVI PPAVEKDTIS MTIRKPSSQS FSLEELANKG LFSLCEQVSF TPLNDYHARF NELKIIEHNL ANAYCNKDFV SFLNQAVKCQ KNILIAGKTG SGKTTLSKAL IAKIPQDERI ITIEDTQELV IPHPNHVSMI YSKDGQGLAS VGPKELLESS LRMRPDRILL QELRDGTAFY YIRNVNSGHP GSITTVHAST ALAAFEQMTL LVKESEGGGD LERDDIRGLL ISMIDIIIQC KRVEGKFKVT EIYYDPF
Validated NT Sequence
ttgaagggat cataataaat ttctgtgacc ttaaattttc cttcaacccg tttgcattgg atgataatat caatcattga aatcaataaa cctcgaatat catcacgctc taaatccccc cctccttcac tttctttgac caaaagggtc atttgttcaa aggctgcaag ggctgttgat gcatgaacgg tcgtaatgga acctgggtgg cctgaattga cattgcggat ataataaaag gctgtaccat ctcgaagctc ttgcaaaaga atacgatcag gacgcatccg taaagatgat tcaagtaatt ctttgggacc aacagaagct aagccttgtc catcttttga atagatcatc gagacatggt tagggtgcgg tatcaccaat tcctgtgtgt cttcaatggt gataatacgc tcgtcttgag gaatttttgc aattaatgcc tttgataatg ttgttttacc cgaaccagtt tttcctgcaa tcagaatatt tttttggcat tttacagcct gatttaaaaa ggaaacaaag tctttattgc agtaggcatt ggctagatta tgctcgatga ttttgagttc gttaaaacgc gcatgataat cattcaatgg tgtgaaagag acctgctcac ataatgagaa gagaccttta ttggcaagct cttcgagaga aaaactctgc gacgatggtt tacgaattgt catactaatt gtgtcttttt cgacagcggg tggaatgaca atttgaatgc gctcattgcc tgggagagtc gctgataata ttggattcct atctgatata ttttgcttag aataggacgc gacaactttc gcaattccca tcagttcatt aaaagagaga gcaggtgctt ctatcgtttt ccacccatca attccttccg tcatcacttg atagggacga ttgataacaa tttcaaaaag accctcatct ctcaaaaaag tactgatagg ttcaagtttt gtgagaacaa tcgcgactgc ttcatcattc atggtgcgta aatttggatt catatggtgg tggtggtg
Expected Protein Sequence
MAHHHHHHMN PNLRTMNDEA VAIVLTKLEP ISTFLRDEGL FEIVINRPYQ VMTEGIDGWK TIEAPALSFN ELMGIAKVVA SYSKQNISDR NPILSATLPG NERIQIVIPP AVEKDTISMT IRKPSSQSFS LEELANKGLF SLCEQVSFTP LNDYHARFNE LKIIEHNLAN AYCNKDFVSF LNQAVKCQKN ILIAGKTGSG KTTLSKALIA KIPQDERIIT IEDTQELVIP HPNHVSMIYS KDGQGLASVG PKELLESSLR MRPDRILLQE LRDGTAFYYI RNVNSGHPGS ITTVHASTAL AAFEQMTLLV KESEGGGDLE RDDIRGLLIS MIDIIIQCKR VEGKFKVTEI YYDPFKQRNI FGGD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc caaatttacg caccatgaat gatgaagcag tcgcgattgt tctcacaaaa cttgaaccta tcagtacttt tttgagagat gagggtcttt ttgaaattgt tatcaatcgt ccctatcaag tgatgacgga aggaattgat gggtggaaaa cgatagaagc acctgctctc tcttttaatg aactgatggg aattgcgaaa gttgtcgcgt cctattctaa gcaaaatata tcagatagga atccaatatt atcagcgact ctcccaggca atgagcgcat tcaaattgtc attccacccg ctgtcgaaaa agacacaatt agtatgacaa ttcgtaaacc atcgtcgcag agtttttctc tcgaagagct tgccaataaa ggtctcttct cattatgtga gcaggtctct ttcacaccat tgaatgatta tcatgcgcgt tttaacgaac tcaaaatcat cgagcataat ctagccaatg cctactgcaa taaagacttt gtttcctttt taaatcaggc tgtaaaatgc caaaaaaata ttctgattgc aggaaaaact ggttcgggta aaacaacatt atcaaaggca ttaattgcaa aaattcctca agacgagcgt attatcacca ttgaagacac acaggaattg gtgataccgc accctaacca tgtctcgatg atctattcaa aagatggaca aggcttagct tctgttggtc ccaaagaatt acttgaatca tctttacgga tgcgtcctga tcgtattctt ttgcaagagc ttcgagatgg tacagccttt tattatatcc gcaatgtcaa ttcaggccac ccaggttcca ttacgaccgt tcatgcatca acagcccttg cagcctttga acaaatgacc cttttggtca aagaaagtga aggagggggg gatttagagc gtgatgatat tcgaggttta ttgatttcaa tgattgatat tatcatccaa tgcaaacggg ttgaaggaaa atttaaggtc acagaaattt attatgatcc cttcaagcaa cgtaacattt ttggcggtga ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BatrA.00720.a.B1.PS01696
PURIFICATION DATe: 12/6/2012
CONCENTRATION: 24mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MNPNLRTMND EAVAIVLTKL EPISTFLRDE GLFEIVINRP YQVMTEGIDG WKTIEAPALS FNELMGIAKV VASYSKQNIS DRNPILSATL PGNERIQIVI PPAVEKDTIS MTIRKPSSQS FSLEELANKG LFSLCEQVSF TPLNDYHARF NELKIIEHNL ANAYCNKDFV SFLNQAVKCQ KNILIAGKTG SGKTTLSKAL IAKIPQDERI ITIEDTQELV IPHPNHVSMI YSKDGQGLAS VGPKELLESS LRMRPDRILL QELRDGTAFY YIRNVNSGHP GSITTVHAST ALAAFEQMTL LVKESEGGGD LERDDIRGLL ISMIDIIIQC KRVEGKFKVT EIYYDPF
Validated NT Sequence
ttgaagggat cataataaat ttctgtgacc ttaaattttc cttcaacccg tttgcattgg atgataatat caatcattga aatcaataaa cctcgaatat catcacgctc taaatccccc cctccttcac tttctttgac caaaagggtc atttgttcaa aggctgcaag ggctgttgat gcatgaacgg tcgtaatgga acctgggtgg cctgaattga cattgcggat ataataaaag gctgtaccat ctcgaagctc ttgcaaaaga atacgatcag gacgcatccg taaagatgat tcaagtaatt ctttgggacc aacagaagct aagccttgtc catcttttga atagatcatc gagacatggt tagggtgcgg tatcaccaat tcctgtgtgt cttcaatggt gataatacgc tcgtcttgag gaatttttgc aattaatgcc tttgataatg ttgttttacc cgaaccagtt tttcctgcaa tcagaatatt tttttggcat tttacagcct gatttaaaaa ggaaacaaag tctttattgc agtaggcatt ggctagatta tgctcgatga ttttgagttc gttaaaacgc gcatgataat cattcaatgg tgtgaaagag acctgctcac ataatgagaa gagaccttta ttggcaagct cttcgagaga aaaactctgc gacgatggtt tacgaattgt catactaatt gtgtcttttt cgacagcggg tggaatgaca atttgaatgc gctcattgcc tgggagagtc gctgataata ttggattcct atctgatata ttttgcttag aataggacgc gacaactttc gcaattccca tcagttcatt aaaagagaga gcaggtgctt ctatcgtttt ccacccatca attccttccg tcatcacttg atagggacga ttgataacaa tttcaaaaag accctcatct ctcaaaaaag tactgatagg ttcaagtttt gtgagaacaa tcgcgactgc ttcatcattc atggtgcgta aatttggatt catatggtgg tggtggtg
Expressed Protein Sequence
MAHHHHHHMN PNLRTMNDEA VAIVLTKLEP ISTFLRDEGL FEIVINRPYQ VMTEGIDGWK TIEAPALSFN ELMGIAKVVA SYSKQNISDR NPILSATLPG NERIQIVIPP AVEKDTISMT IRKPSSQSFS LEELANKGLF SLCEQVSFTP LNDYHARFNE LKIIEHNLAN AYCNKDFVSF LNQAVKCQKN ILIAGKTGSG KTTLSKALIA KIPQDERIIT IEDTQELVIP HPNHVSMIYS KDGQGLASVG PKELLESSLR MRPDRILLQE LRDGTAFYYI RNVNSGHPGS ITTVHASTAL AAFEQMTLLV KESEGGGDLE RDDIRGLLIS MIDIIIQCKR VEGKFKVTEI YYDPFKQRNI FGGD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATC CAAATTTACG CACCATGAAT GATGAAGCAG TCGCGATTGT TCTCACAAAA CTTGAACCTA TCAGTACTTT TTTGAGAGAT GAGGGTCTTT TTGAAATTGT TATCAATCGT CCCTATCAAG TGATGACGGA AGGAATTGAT GGGTGGAAAA CGATAGAAGC ACCTGCTCTC TCTTTTAATG AACTGATGGG AATTGCGAAA GTTGTCGCGT CCTATTCTAA GCAAAATATA TCAGATAGGA ATCCAATATT ATCAGCGACT CTCCCAGGCA ATGAGCGCAT TCAAATTGTC ATTCCACCCG CTGTCGAAAA AGACACAATT AGTATGACAA TTCGTAAACC ATCGTCGCAG AGTTTTTCTC TCGAAGAGCT TGCCAATAAA GGTCTCTTCT CATTATGTGA GCAGGTCTCT TTCACACCAT TGAATGATTA TCATGCGCGT TTTAACGAAC TCAAAATCAT CGAGCATAAT CTAGCCAATG CCTACTGCAA TAAAGACTTT GTTTCCTTTT TAAATCAGGC TGTAAAATGC CAAAAAAATA TTCTGATTGC AGGAAAAACT GGTTCGGGTA AAACAACATT ATCAAAGGCA TTAATTGCAA AAATTCCTCA AGACGAGCGT ATTATCACCA TTGAAGACAC ACAGGAATTG GTGATACCGC ACCCTAACCA TGTCTCGATG ATCTATTCAA AAGATGGACA AGGCTTAGCT TCTGTTGGTC CCAAAGAATT ACTTGAATCA TCTTTACGGA TGCGTCCTGA TCGTATTCTT TTGCAAGAGC TTCGAGATGG TACAGCCTTT TATTATATCC GCAATGTCAA TTCAGGCCAC CCAGGTTCCA TTACGACCGT TCATGCATCA ACAGCCCTTG CAGCCTTTGA ACAAATGACC CTTTTGGTCA AAGAAAGTGA AGGAGGGGGG GATTTAGAGC GTGATGATAT TCGAGGTTTA TTGATTTCAA TGATTGATAT TATCATCCAA TGCAAACGGG TTGAAGGAAA ATTTAAGGTC ACAGAAATTT ATTATGATCC CTTCAAGCAA CGTAACATTT TTGGCGGTGA CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT