SSGCID: Target Page

BaspA.18423.a

Filamentation induced by cAMP protein Fic (VirB-translocated Bep effector protein)

CENTER ID:	BaspA.18423.a
ORGANISM:	Bartonella sp. 1-1C
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaspA.18423.a.B1.GE35943	full length		1	348

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaspA.18423.a.B1.PS01673	full length		1	348

External Resources

RESOURCE	REFERENCE ID
UniProt:	E6YU37

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQINTLKITQ ELLALITEID EFKGAWRAFK TLAPERLNAL RHIAAVESIG SSTRIEGNKL TDREIEKLLL NSEIKSFKNR DEQEVIGYAK VMEIIFQSWK DIPITENHIK QLHRDLLYES DKDERHRGEY KTLRNDVTAF NSEGKMVGIV FETATPFDTP YRMQELIDWF NKTNELNNLH PLLMIAIFNV IFLAIHPFQD GNGRLSRILT TLLLLQKGYV YVPYSSLESI IENNKESYYL ALNQTQKTIY TKTPNWEPWI LFFLRALQKQ KRQLEMKIER EKNTLLALSE LALNILDYVN NHGRVTTRDM VREIGASPNT LKATFTSLVE KGFLTRHGGG RSTWYSLP

NT Sequence

atgcaaataa atacactcaa aatcacacaa gaattgttag cccttattac tgaaattgat gagttcaaag gtgcatggcg cgctttcaaa actcttgctc cagaacgttt gaatgctctg cgtcatattg cagctgttga aagtattgga tcttctactc gtattgaagg aaacaaatta acagaccgtg agattgaaaa gcttttatta aattcagaaa ttaaaagttt taagaaccgt gatgaacaag aagttattgg ttatgcaaaa gttatggaaa taatctttca atcttggaaa gatattccta ttacagaaaa tcatattaaa cagctgcatc gtgatctttt atatgagagc gataaagatg aacgtcatag aggggaatat aagacattac gtaatgatgt aacagcattc aattctgaag ggaaaatggt tggtattgtt tttgaaacag caacaccatt tgacacacca taccgaatgc aagaacttat cgattggttt aataaaacca atgaattaaa taatcttcat cctttactta tgattgctat ttttaatgtc attttcttag ctattcatcc tttccaagat ggaaatggac gtttaagtcg tatcttaacg accttacttt tgttacaaaa aggttatgtt tacgtaccat attcatcact tgaaagcatt attgagaata ataaagaaag ctactactta gctttgaatc aaacgcaaaa aacaatttat actaagacac ccaattggga accttggata ctcttttttt tacgtgctct acaaaaacaa aaacgccaat tagaaatgaa aatagaacgt gaaaaaaata ctcttttggc tctatctgaa ttagctctta atattcttga ttatgttaat aatcatgggc gtgttacaac tcgcgatatg gttcgtgaaa ttggagcaag ccccaataca cttaaagcaa cttttacatc attagttgaa aaaggatttt tgacgcgtca tggaggagga cgatcaactt ggtatagctt accttaa

Details for BaspA.18423.a.B1.GE35943

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	41kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	51

Validated AA Sequence

MEIIFQSWKD IPITENHIKQ LHRDLLYESD KDERHRGEYK TLRNDVTAFN SEGKMVGIVF ETATPFDTPY RMQELIDWFN KTNELNNLHP LLMIAIFNVI FLAIHPFQDG NGRLSRILTT LLLLQKGYVY VPYSSLESII ENNKESYYLA LNQTQKTIYT KTPNWEPWIL FFFTCSTKTK TPX

Validated NT Sequence

angatgnaaa agnngcttna agtgtanngg ggcttgnncc aatttcncga accanatcgc gagnngtaac acgcccatga ttattaacat antcaagaat attaagagnt aattcagata gagccaaaag agtatttttt tcacgttcta ttttcatttc tanntnggcg tttttgtttt tgtagagcac gtaaaaaaaa agagtatcca aggttcccaa ttgggtgtct tagtataaat tgttttttgc gtttgattca aagctaagta gtagctttct ttattattct caataatgct ttcaagtgat gaatatggta cgtaaacata acctttttgt aacaaaagta aggtcgttaa gatacgactt aaacgtccat ttccatcttg gaaaggatga atagctaaga aaatgacatt aaaaatagca atcataagta aaggatgaag attatttaat tcattggttt tattaaacca atcgataagt tcttgcattc ggtatggtgt gtcaaatggt gttgctgttt caaaaacaat accaaccatt ttcccttcag aattgaatgc tgttacatca ttacgtaatg tcttatattc ccctctatga cgttcatctt tatcgctctc atataaaaga tcacgatgca gctgtttaat atgattttct gtaataggaa tatctttcca agattgaaag attatttcca taacttttgc ataaccaata acttcttgtt catcacggtt cttaaaactt ttaatttctg aatttaataa aagcttttca atctcacggt ctgttaattt gtttccttca atacgagtag aagatccaat actttcaaca gctgcaatat gacgcagagc attcaaacgt tctggagcaa gagttttgaa agcgcgccat gcacctttga actcatcaat ttcagtaata agggctaaca attcttgtgt gattttgagt gtatt

Expected Protein Sequence

MAHHHHHHMQ INTLKITQEL LALITEIDEF KGAWRAFKTL APERLNALRH IAAVESIGSS TRIEGNKLTD REIEKLLLNS EIKSFKNRDE QEVIGYAKVM EIIFQSWKDI PITENHIKQL HRDLLYESDK DERHRGEYKT LRNDVTAFNS EGKMVGIVFE TATPFDTPYR MQELIDWFNK TNELNNLHPL LMIAIFNVIF LAIHPFQDGN GRLSRILTTL LLLQKGYVYV PYSSLESIIE NNKESYYLAL NQTQKTIYTK TPNWEPWILF FLRALQKQKR QLEMKIEREK NTLLALSELA LNILDYVNNH GRVTTRDMVR EIGASPNTLK ATFTSLVEKG FLTRHGGGRS TWYSLP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa taaatacact caaaatcaca caagaattgt tagcccttat tactgaaatt gatgagttca aaggtgcatg gcgcgctttc aaaactcttg ctccagaacg tttgaatgct ctgcgtcata ttgcagctgt tgaaagtatt ggatcttcta ctcgtattga aggaaacaaa ttaacagacc gtgagattga aaagctttta ttaaattcag aaattaaaag ttttaagaac cgtgatgaac aagaagttat tggttatgca aaagttatgg aaataatctt tcaatcttgg aaagatattc ctattacaga aaatcatatt aaacagctgc atcgtgatct tttatatgag agcgataaag atgaacgtca tagaggggaa tataagacat tacgtaatga tgtaacagca ttcaattctg aagggaaaat ggttggtatt gtttttgaaa cagcaacacc atttgacaca ccataccgaa tgcaagaact tatcgattgg tttaataaaa ccaatgaatt aaataatctt catcctttac ttatgattgc tatttttaat gtcattttct tagctattca tcctttccaa gatggaaatg gacgtttaag tcgtatctta acgaccttac ttttgttaca aaaaggttat gtttacgtac catattcatc acttgaaagc attattgaga ataataaaga aagctactac ttagctttga atcaaacgca aaaaacaatt tatactaaga cacccaattg ggaaccttgg atactctttt ttttacgtgc tctacaaaaa caaaaacgcc aattagaaat gaaaatagaa cgtgaaaaaa atactctttt ggctctatct gaattagctc ttaatattct tgattatgtt aataatcatg ggcgtgttac aactcgcgat atggttcgtg aaattggagc aagccccaat acacttaaag caacttttac atcattagtt gaaaaaggat ttttgacgcg tcatggagga ggacgatcaa cttggtatag cttaccttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BaspA.18423.a.B1.PS01673

PURIFICATION DATe:	11/6/2012
CONCENTRATION:	8.07mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	95
PERCENT COVERAGE:	51

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAA TAAATACACT CAAAATCACA CAAGAATTGT TAGCCCTTAT TACTGAAATT GATGAGTTCA AAGGTGCATG GCGCGCTTTC AAAACTCTTG CTCCAGAACG TTTGAATGCT CTGCGTCATA TTGCAGCTGT TGAAAGTATT GGATCTTCTA CTCGTATTGA AGGAAACAAA TTAACAGACC GTGAGATTGA AAAGCTTTTA TTAAATTCAG AAATTAAAAG TTTTAAGAAC CGTGATGAAC AAGAAGTTAT TGGTTATGCA AAAGTTATGG AAATAATCTT TCAATCTTGG AAAGATATTC CTATTACAGA AAATCATATT AAACAGCTGC ATCGTGATCT TTTATATGAG AGCGATAAAG ATGAACGTCA TAGAGGGGAA TATAAGACAT TACGTAATGA TGTAACAGCA TTCAATTCTG AAGGGAAAAT GGTTGGTATT GTTTTTGAAA CAGCAACACC ATTTGACACA CCATACCGAA TGCAAGAACT TATCGATTGG TTTAATAAAA CCAATGAATT AAATAATCTT CATCCTTTAC TTATGATTGC TATTTTTAAT GTCATTTTCT TAGCTATTCA TCCTTTCCAA GATGGAAATG GACGTTTAAG TCGTATCTTA ACGACCTTAC TTTTGTTACA AAAAGGTTAT GTTTACGTAC CATATTCATC ACTTGAAAGC ATTATTGAGA ATAATAAAGA AAGCTACTAC TTAGCTTTGA ATCAAACGCA AAAAACAATT TATACTAAGA CACCCAATTG GGAACCTTGG ATACTCTTTT TTTTACGTGC TCTACAAAAA CAAAAACGCC AATTAGAAAT GAAAATAGAA CGTGAAAAAA ATACTCTTTT GGCTCTATCT GAATTAGCTC TTAATATTCT TGATTATGTT AATAATCATG GGCGTGTTAC AACTCGCGAT ATGGTTCGTG AAATTGGAGC AAGCCCCAAT ACACTTAAAG CAACTTTTAC ATCATTAGTT GAAAAAGGAT TTTTGACGCG TCATGGAGGA GGACGATCAA CTTGGTATAG CTTACCTTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT