BaspA.18410.a

Bep9 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaspA.18410.a
ORGANISM: Bartonella sp. 1-1C
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaspA.18410.a.B3.GE37373 BID 478 615
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: E6YWF3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLEEKKKML NDVLISPKEL PSLTDDQIIQ SVLQDSFVKE KKEELESLSK TVFKSWNTLP FLINRINEDP DSARILVNKL RSSPLSFSKL AGFKIFGFKS PKRIKAEKNI FKLSLVVQQY GNVVQESRDT IIYHHNLEQA RCARKVLIPS DDIQYLLDLP YDMLLNVFKN QDTYQLGKKI LLFLNSVDMR LSANELQMLE AHKTDKFAKS MGISDIEASR MIDVVTKATK VLNKINIFRS NTHKISEAQT QELRSEGVSS GIYTNYNPNE AQTQRLRSEG VSSGIYTNYN PNEAQTQWLR SEEEYSAIYD LNEAQTQELR NEEEYSGIYE SYDPHEAQTQ ELRNEEEYSG IYANYDPNEA QTQELRNEEE YSGIYANCDP NRPYARILKT RKEKSDIHDN DDIYDNKDIY DSANIHDNDD IYDNKDIYDS ANIHDNDDIY DNKDIYDNPN AQTQELRSEE ENFGIYDTAE AQSSKRFLIP AKALAPLTPN EITDKIKINP VVQEHAEKVR DLSELVFGDR ELLNSHLAQI IKYPKLGESY SQTLKTSPES IAKFAGSEKW GVGNLRYREA EKNVPELCQA IEEYGNIVEV HHKQIIKNHE KEQKQIHKSV AMPSNEIQRL LNLPSDMRKN ILREKNTSQL NKELSVFLNQ VNFRLSIHEH MAIKEDDYQT LASSIKVSEV QAREIINFVK EAKTCQQEIK DIIFPQKKRA LAVAS
NT Sequence
atgaatttgg aagaaaagaa aaaaatgctt aatgatgttc ttatttcacc aaaagaattg ccttctctaa cagatgatca gattattcaa tcggtcttac aagactcttt tgttaaagaa aaaaaagaag aacttgaaag tttgtcaaaa actgtattta aaagctggaa tacattacct tttttgataa accggataaa tgaagatcca gattctgcaa gaatattggt gaataaactt agatcctctc ctttatcctt ctccaagctt gcaggtttta aaatatttgg atttaaaagt ccaaaacgta taaaggctga aaaaaatatt tttaaactta gtctagtagt tcaacaatac ggaaatgttg tgcaagaatc tagagataca atcatttacc atcataactt agaacaagca cgttgtgcac gaaaagtttt aatacctagt gatgatatac aatatcttct tgatttacca tatgacatgc tgttgaatgt ttttaaaaat caagatactt accaacttgg taaaaagata ttgttgtttc tcaattcagt tgatatgcgt ctttcagcaa acgagcttca aatgcttgaa gcgcataaga cagataagtt tgctaaaagt atgggtatat cagacattga agcgtcgaga atgatagatg ttgtcacaaa agccacaaaa gtactgaaca aaattaacat attccggtcg aacacgcata aaataagtga agctcaaaca caagagttga gaagtgaagg agtgtcctcg ggtatctaca caaattataa tccaaatgaa gctcaaacac agaggttgag aagtgaagga gtgtcctcgg gtatctacac aaattataat ccaaatgaag ctcaaacaca gtggttgaga agcgaagaag aatactcagc tatctatgat ctgaatgaag ctcaaacgca agagctgaga aatgaagaag agtactccgg tatctatgaa agctatgatc cgcatgaagc tcaaacgcaa gagctgagaa atgaagaaga gtactccggt atctatgcaa attatgatcc gaatgaagct caaacacaag agctgagaaa tgaagaagag tactccggta tctatgcaaa ttgtgatcca aatagacctt acgcgcggat attaaagaca agaaaagaga aatcggatat tcatgataat gacgatatct atgataataa ggatatctat gacagtgcca atattcatga taatgacgat atctatgata ataaggatat ctatgacagt gccaatattc atgataatga cgatatctat gataataagg atatctatga taatccgaat gctcaaacac aggagttgag gagcgaggaa gagaacttcg gtatctatga tactgctgaa gcacagagtt ctaaacgttt tcttattccg gcaaaagcat tagcgccttt aaccccaaat gaaattactg ataaaattaa aattaatcct gttgttcaag agcatgcaga aaaagttcga gatctttcag aactggtatt tggagaccga gaattattga acagccatct cgcacaaatt attaagtatc caaaattagg agaatcatat agccaaacac tcaaaacctc tcctgaatcc attgctaaat ttgccggctc tgaaaagtgg ggagtaggaa atttaagata tagagaagct gaaaaaaatg tcccagaact ctgtcaagca attgaagaat atggaaacat tgtggaagta catcataaac aaattattaa aaaccatgaa aaagaacaaa aacagatcca caaatcggta gcaatgccta gtaatgaaat acagcgtctt ttaaatctac caagtgatat gcgaaaaaat attttaagag aaaaaaatac ttctcaactt aacaaggaac tttccgtttt tttaaatcaa gttaattttc gtctttcaat acatgaacat atggcaatta aagaagatga ttatcagaca cttgctagca gtattaaggt atcagaagtt caagcacgcg aaattataaa ttttgttaaa gaagcaaaaa catgtcaaca agaaattaaa gatataatat ttccgcagaa gaaaagagcg ttagctgtag ctagttaa
Details for BaspA.18410.a.B3.GE37373
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML IPAKALAPLT PNEITDKIKI NPVVQEHAEK VRDLSELVFG DRELLNSHLA QIIKYPKLGE SYSQTLKTSP ESIAKFAGSE KWGVGNLRYR EAEKNVPELC QAIEEYGNIV EVHHKQIIKN HEKEQKQIHK SVAMPSN
Validated NT Sequence
attttnntta nctttaagaa ggaganatac catggctcac caccaccacc accatatgct tattccggca aaagcattag cgcctttaac cccaaatgaa attactgata aaattaaaat taatcctgtt gttcaagagc atgcagaaaa agttcgagat ctttcagaac tggtatttgg agaccgagaa ttattgaaca gccatctcgc acaaattatt aagtatccaa aattaggaga atcatatagc caaacactca aaacctctcc tgaatccatt gctaaatttg ccggctctga aaagtgggga gtaggaaatt taagatatag agaagctgaa aaaaatgtcc cagaactctg tcaagcaatt gaagaatatg gaaacattgt ggaagtacat cataaacaaa ttattaaaaa ccatgaaaaa gaacaaaaac agatccacaa atcggtagca atgcctagta attaagtgag taagatagga tccggctgct aacaagcnnc gaaagangnn ttnnnnnnnn ctgcgcnnnn nnnnnnncnn tannntnnnn nnntngccgc cacnacannn nnncnngnga catgngngnn tnnngangnn ncananncgn nnannnngan nnnnngnnnn nnntntnnnn nnannnnnnn ntccnannnn gcgnnnnnnc tnannnnnnn cnnnacannn gnnnnngaag annanntcnn nnnnnnnnnn aannnnnnng annnnnnnnn acccggnnnn nnnnnnnngn nnnnnnnnng nnnnnnnnnn nngnnnngnn nnnnnnnnnn annnnnncga nnnnnnnnnn cnnnnnnnaa nngnnaannn nnnngnnncn nnnnnncntn nnnnnnnnnn nnnnccnnnn ncnncnnnng nnnnnnnnng
Expected Protein Sequence
MAHHHHHHLI PAKALAPLTP NEITDKIKIN PVVQEHAEKV RDLSELVFGD RELLNSHLAQ IIKYPKLGES YSQTLKTSPE SIAKFAGSEK WGVGNLRYRE AEKNVPELCQ AIEEYGNIVE VHHKQIIKNH EKEQKQIHKS VAMPSN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcttattc cggcaaaagc attagcgcct ttaaccccaa atgaaattac tgataaaatt aaaattaatc ctgttgttca agagcatgca gaaaaagttc gagatctttc agaactggta tttggagacc gagaattatt gaacagccat ctcgcacaaa ttattaagta tccaaaatta ggagaatcat atagccaaac actcaaaacc tctcctgaat ccattgctaa atttgccggc tctgaaaagt ggggagtagg aaatttaaga tatagagaag ctgaaaaaaa tgtcccagaa ctctgtcaag caattgaaga atatggaaac attgtggaag tacatcataa acaaattatt aaaaaccatg aaaaagaaca aaaacagatc cacaaatcgg tagcaatgcc tagtaattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at