SSGCID: Target Page

BaspA.18404.c

Effector protein yopJ (Yop-like family protein)

CENTER ID:	BaspA.18404.c
ORGANISM:	Bartonella sp. 1-1C
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaspA.18404.c.B1.GE35940	full length		1	303

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	E6YW76

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRLRDLLGK VFHKKNKNDV VFEKKEKAVL RRKHNFIFGK DEPLKTTIMR LENDITHGSW YDTLYGELDF AMMPSLIEKA NKKYPLMSLK FSATPEDVPI LIKNTIDDKI QSSRLIVSLG DEKIHFAVLD HQTINDRVSL ILFETVGFKH MKPAVLAMRV KMAIEESQLP NCHFSIAEMD IQRSASECGI FSLALAKKLY READKLEKLH RDNINNVLCK SDFFVSYDEL DKYLPAAFYK HTQSITRLNE YIESNPKAKR EIINKKGEVI LERFDKNSVM IDNKKVSCSL HKKRVNEYKS LIR

NT Sequence

atgtccagat taagagattt actaggaaaa gtttttcata aaaaaaataa aaacgatgtt gtgtttgaaa aaaaagaaaa agctgtactt agaagaaagc acaattttat atttgggaaa gatgagccat taaaaaccac tattatgcgt ctggaaaacg acattacgca tggtagttgg tatgatacgc tttatggaga gttggatttt gcaatgatgc cttctctgat agagaaagca aataaaaaat atccactgat gagccttaag ttttctgcaa cccctgagga tgttcctata ttaataaaaa acacaataga tgataagatt caatcttcta gattaatagt tagtctagga gatgaaaaaa tccattttgc agtactcgat catcaaacta ttaatgacag ggtatctttg atattgtttg aaactgtagg attcaagcat atgaagccgg cggtactagc aatgagagta aaaatggcta ttgaagagag tcaattacct aattgtcatt tttctatagc agaaatggat attcaacgga gtgcttctga atgtgggatt tttagtttgg ctctagctaa aaagctttat cgtgaagcgg acaaattaga aaaactacac agagataata ttaacaatgt cttatgtaaa tcagattttt ttgtatctta tgatgagcta gataagtatc ttccagccgc tttttacaaa catacgcaga gcataacccg ccttaatgaa tatatagaat caaatccgaa agctaaacga gaaattatta acaagaaggg cgaagttatt ttggagaggt ttgataagaa ttcagtcatg atagataata aaaaggtatc ttgttcgcta cataaaaaaa gagttaatga atataaatct ttgataaggt aa

Details for BaspA.18404.c.B1.GE35940

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS RLRDLLGKVF HKKNKNDVVF EKKEKAVLRR KHNFIFGKDE PLKTTIMRLE NDITHGSWYD TLYGELDFAM MPSLIEKANK KYPLMSLKFS ATPEDVPILI KNTIDDKIQS SRLIVSLGDE KIHFAVLDHQ TINDRVSLIL FETVGFKHMK PAVLAMRVKM AIEESQLPNC HFSIAEMDIQ RSASECGIFS LALAKKLYRE ADKLEKLHRD NINNVLCKSD FFVSYDELDK YLPAAFYKHT QSITRLNEYI ESNPKAKREI INKKGEVILX RFDKNSVMID NKKVSCSLXK KXVNEYKSLI R

Validated NT Sequence

tttgtttnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatgtccag attaagagat ttactaggaa aagtttttca taaaaaaaat aaaaacgatg ttgtgtttga aaaaaaagaa aaagctgtac ttagaagaaa gcacaatttt atatttggga aagatgagcc attaaaaacc actattatgc gtctggaaaa cgacattacg catggtagtt ggtatgatac gctttatgga gagttggatt ttgcaatgat gccttctctg atagagaaag caaataaaaa atatccactg atgagcctta agttttctgc aacccctgag gatgttccta tattaataaa aaacacaata gatgataaga ttcaatcttc tagattaata gttagtctag gagatgaaaa aatccatttt gcagtactcg atcatcaaac tattaatgac agggtatctt tgatattgtt tgaaactgta ggattcaagc atatgaagcc ggcggtacta gcaatgagag taaaaatggc tattgaagag agtcaattac ctaattgtca tttttctata gcagaaatgg atattcaacg gagtgcttct gaatgtggga tttttagttt ggctctagct aaaaagcttt atcgtgaagc ggacaaatta gaaaaactac acagagataa tattaacaat gtcttatgta aatcagattt ttttgtatct tatgatgagc tagataagta tcttccagcc gctttttaca aacatacgca gagcataacc cgccttaatg aatatataga atcaaatccg aaagctaaac gagaaattat taacaagaag ggcgaagtta ttttgnagag gtttgataag aattcagtca tgatagataa taaaaaggta tcttgttcgc tacntaaaaa aanagttaat gaatataaat ctttgataag gtaagtgagt aananaggat ccnnnnnnnn nnncccccc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca gattaagaga tttactagga aaagtttttc ataaaaaaaa taaaaacgat gttgtgtttg aaaaaaaaga aaaagctgta cttagaagaa agcacaattt tatatttggg aaagatgagc cattaaaaac cactattatg cgtctggaaa acgacattac gcatggtagt tggtatgata cgctttatgg agagttggat tttgcaatga tgccttctct gatagagaaa gcaaataaaa aatatccact gatgagcctt aagttttctg caacccctga ggatgttcct atattaataa aaaacacaat agatgataag attcaatctt ctagattaat agttagtcta ggagatgaaa aaatccattt tgcagtactc gatcatcaaa ctattaatga cagggtatct ttgatattgt ttgaaactgt aggattcaag catatgaagc cggcggtact agcaatgaga gtaaaaatgg ctattgaaga gagtcaatta cctaattgtc atttttctat agcagaaatg gatattcaac ggagtgcttc tgaatgtggg atttttagtt tggctctagc taaaaagctt tatcgtgaag cggacaaatt agaaaaacta cacagagata atattaacaa tgtcttatgt aaatcagatt tttttgtatc ttatgatgag ctagataagt atcttccagc cgctttttac aaacatacgc agagcataac ccgccttaat gaatatatag aatcaaatcc gaaagctaaa cgagaaatta ttaacaagaa gggcgaagtt attttggaga ggtttgataa gaattcagtc atgatagata ataaaaaggt atcttgttcg ctacataaaa aaagagttaa tgaatataaa tctttgataa ggtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat