SSGCID: Target Page

BaspA.18404.b

Effector protein yopJ (Yop-like family protein)

CENTER ID:	BaspA.18404.b
ORGANISM:	Bartonella sp. 1-1C
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaspA.18404.b.B1.GE35939	full length		1	218

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	E6YW79

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNLRLVTTLK DLGASMQKTI GDGIQSSRFI VNVLTSAMPH FVVIDHKTIN NKLSFVLFEC TRCNNEVSFV LISKVKRAIE GFQLPDFYCS IVEMDIQRSM SECGIFSLAL AKKLYLRSDK LEKLHRDNIK GDRWDRDVYL SYDLLDTYLP IDFYKHVQGF RRLEEYVKKN PGSEKEIVNK KNETIFERFE RHTMVKRGRN MSASAHKKRI TEYKSLMR

NT Sequence

atgaatctta ggcttgttac gacattgaaa gatttgggcg catctatgca aaaaacaatc ggtgatggga ttcaatcttc gaggtttata gtgaatgtac tgactagtgc tatgccccat tttgtagtaa ttgatcataa aactatcaac aataagcttt cctttgtatt atttgagtgc acaagatgta ataatgaggt ttcttttgta ctaatatcga aggtaaaaag agccattgaa ggtttccaat tgcctgattt ttattgttct attgtagaaa tggatattca gcgaagtatg tctgaatgtg gaatttttag tttagctctc gctaaaaaac tttatcttcg atctgataaa ttagaaaaac tgcatagaga taatattaaa ggtgatcggt gggatagaga tgtttattta tcttatgatt tattggatac atatcttcca attgactttt ataaacatgt gcaaggtttt agacgtcttg aggaatatgt gaaaaaaaat ccaggatctg aaaaagaaat cgttaacaaa aaaaatgaaa ccatttttga gaggtttgaa agacatacaa tggtaaaaag aggtagaaat atgtctgcat cagcacacaa aaaaaggatt actgaatata aatcattaat gagatga

Details for BaspA.18404.b.B1.GE35939

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN LRLVTTLKDL GASMQKTIGD GIQSSRFIVN VLTSAMPHFV VIDHKTINNK LSFVLFECTR CNNEVSFVLI SKVKRAIEGF QLPDFYCSIV EMDIQRSMSE CGIFSLALAK KLYLRSDKLE KLHRDNIKGD RWDRDVYLSY DLLDTYLPID FYKHVQGFRR LEEYVKKNPG SEKEIVNKKN ETIFERFERH TMVKRGRNMS ASAHKKRITE YKSLMR

Validated NT Sequence

tttntnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgaatct taggcttgtt acgacattga aagatttggg cgcatctatg caaaaaacaa tcggtgatgg gattcaatct tcgaggttta tagtgaatgt actgactagt gctatgcccc attttgtagt aattgatcat aaaactatca acaataagct ttcctttgta ttatttgagt gcacaagatg taataatgag gtttcttttg tactaatatc gaaggtaaaa agagccattg aaggtttcca attgcctgat ttttattgtt ctattgtaga aatggatatt cagcgaagta tgtctgaatg tggaattttt agtttagctc tcgctaaaaa actttatctt cgatctgata aattagaaaa actgcataga gataatatta aaggtgatcg gtgggataga gatgtttatt tatcttatga tttattggat acatatcttc caattgactt ttataaacat gtgcaaggtt ttagacgtct tgaggaatat gtgaaaaaaa atccaggatc tgaaaaagaa atcgttaaca aaaaaaatga aaccattttt gagaggtttg aaagacatac aatggtaaaa agaggtagaa atatgtctgc atcagcacac aaaaaaagga ttactgaata taaatcatta atgagataag tgagtaagat aggatccggc tgcnncccc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc ttaggcttgt tacgacattg aaagatttgg gcgcatctat gcaaaaaaca atcggtgatg ggattcaatc ttcgaggttt atagtgaatg tactgactag tgctatgccc cattttgtag taattgatca taaaactatc aacaataagc tttcctttgt attatttgag tgcacaagat gtaataatga ggtttctttt gtactaatat cgaaggtaaa aagagccatt gaaggtttcc aattgcctga tttttattgt tctattgtag aaatggatat tcagcgaagt atgtctgaat gtggaatttt tagtttagct ctcgctaaaa aactttatct tcgatctgat aaattagaaa aactgcatag agataatatt aaaggtgatc ggtgggatag agatgtttat ttatcttatg atttattgga tacatatctt ccaattgact tttataaaca tgtgcaaggt tttagacgtc ttgaggaata tgtgaaaaaa aatccaggat ctgaaaaaga aatcgttaac aaaaaaaatg aaaccatttt tgagaggttt gaaagacata caatggtaaa aagaggtaga aatatgtctg catcagcaca caaaaaaagg attactgaat ataaatcatt aatgagatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at