SSGCID: Target Page

BaspA.17987.a

DNA polymerase III subunit beta (EC 2.7.7.7)

CENTER ID:	BaspA.17987.a
ORGANISM:	Bartonella sp. 1-1C
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaspA.17987.a.B1.GE41240	full length		1	373

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	E6YTH2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRITIGRSQL LKSLSRVHRI VERRSAIPIL SNVLIDTSDG RMQLKATDLD LEIIESSSVN IEQGGAITVP AHLLYDIVRK LPDDSNVILE VNEDQASAMS ITSGCTNFQL QCLPKVDFPE SLPGQFSCRF SLLASRLKYL LDCTQFAMST EETRYYLNGI YFHVIDDDIL KLRLVSTDGH RLAQVDIEAP SGVDGMPGVI IPRKTVGELQ KLLGEEADND VCIELSDTKI RFSVGSVILT SKLIDGVFPE YQRVIPLGND KKLIVNRQDF SAAVDRVSTI SSDRGRAVKL TIEKGVVKLV VNNPDSGSAK DQLVANYVFD PLEIGFNSRY LLDIAAQLSS DEMIFMFADS GAPTLIRGND DKNALYVLMP IRV

NT Sequence

atgcgcatta caattggtcg cagtcagctg cttaaatctc ttagtcgtgt tcaccgtatt gtggagcgtc gtagcgctat tcctattttg tcaaatgtgt tgatagatac aagtgatggg aggatgcaat taaaagcaac agatcttgat ctagaaatca tagaatcttc ttcagtcaat attgaacaag gtggggcgat aactgttcca gcgcatttgc tttatgatat tgttcgcaaa cttcctgatg atagtaacgt tattttagag gttaatgaag atcaggcaag tgctatgtct attacttctg gttgtactaa ttttcagttg cagtgtcttc ctaaggtaga ttttccggaa tcacttccag gtcagtttag ttgtcgcttc tctttgttgg catcaagatt gaaatattta cttgattgta cacagtttgc tatgtctact gaagagacac gttattatct gaatggtatt tattttcatg tcattgatga tgatattttg aagttgcgtt tggtttctac tgatggtcat cgtttggcgc aggttgatat agaagcacct tcgggagttg atggcatgcc aggtgttatt attcctcgta aaaccgtggg agagttgcaa aaacttcttg gggaagaagc tgataatgat gtgtgtatag agctttcaga tacaaagatt cgcttttctg ttgggtctgt aattttaaca tcaaaactaa ttgatggagt atttccagag tatcagcgcg ttattccatt aggaaatgat aaaaagttga ttgtcaatcg gcaagatttt tctgctgcag ttgatcgtgt ctcaacaatt tcaagtgatc gtgggcgtgc agtaaaattg acgattgaaa aaggggttgt aaaacttgta gtgaacaatc ctgattcagg aagtgcaaaa gatcaattgg ttgcaaatta tgtatttgat ccgcttgaga taggtttcaa ttcaagatat cttttagata ttgcagcaca actttcaagt gatgaaatga ttttcatgtt tgctgattct ggagcgccaa cattgattcg tggaaatgat gataaaaatg cgctttatgt gctgatgcct attcgtgttt ag

Details for BaspA.17987.a.B1.GE41240

HARVESTED ON:	data unavailable
SEQUENCED ON:	3/9/2017
EXPECTED MW:	42kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMR ITIGRSQLLK SLSRVHRIVE RRSAIPILSN VLIDTSDGRM QLKATDLDLE IIESSSVNIE QGGAITVPAH LLYDIVRKLP DDSNVILEVN EDQASAMSIT SGCTNFQLQC LPKVDFPESL PGQFSCRFSL LASRLKYLLD CTQFAMSTEE TRYYLNGIYF HVIDDDILKL RLVSTDGHRL AQVDIEAPSG VDGMPGVIIP RKTVGELQKL LGEEADNDVC IELSDTKIRF SVGSVILTSK LIDGVFPEYQ RVIPLGNDKK LIVNRQDFSA AVDRVSTISS DRGRAVKLTI EKGVVKLVVN NPDSGSAKDQ LVANYVFDPL EIGFNSRYLL DIAAQLSSDE MIFMFADSGA PTLIRGNDDK NALYVLMPIR V

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgca ttacaattgg tcgcagtcag ctgcttaaat ctcttagtcg tgttcaccgt attgtggagc gtcgtagcgc tattcctatt ttgtcaaatg tgttgataga tacaagtgat gggaggatgc aattaaaagc aacagatctt gatctagaaa tcatagaatc ttcttcagtc aatattgaac aaggtggggc gataactgtt ccagcgcatt tgctttatga tattgttcgc aaacttcctg atgatagtaa cgttatttta gaggttaatg aagatcaggc aagtgctatg tctattactt ctggttgtac taattttcag ttgcagtgtc ttcctaaggt agattttccg gaatcacttc caggtcagtt tagttgtcgc ttctctttgt tggcatcaag attgaaatat ttacttgatt gtacacagtt tgctatgtct actgaagaga cacgttatta tctgaatggt atttattttc atgtcattga tgatgatatt ttgaagttgc gtttggtttc tactgatggt catcgtttgg cgcaggttga tatagaagca ccttcgggag ttgatggcat gccaggtgtt attattcctc gtaaaaccgt gggagagttg caaaaacttc ttggggaaga agctgataat gatgtgtgta tagagctttc agatacaaag attcgctttt ctgttgggtc tgtaatttta acatcaaaac taattgatgg agtatttcca gagtatcagc gcgttattcc attaggaaat gataaaaagt tgattgtcaa tcggcaagat ttttctgctg cagttgatcg tgtctcaaca atttcaagtg atcgtgggcg tgcagtaaaa ttgacgattg aaaaaggggt tgtaaaactt gtagtgaaca atcctgattc aggaagtgca aaagatcaat tggttgcaaa ttatgtattt gatccgcttg agataggttt caattcaaga tatcttttag atattgcagc acaactttca agtgatgaaa tgattttcat gtttgctgat tctggagcgc caacattgat tcgtggaaat gatgataaaa atgcgcttta tgtgctgatg cctattcgtg tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat