BaspA.17330.j

Bep7 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaspA.17330.j
ORGANISM: Bartonella sp. 1-1C
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaspA.17330.j.B1.GE35936 full length 1 580
BaspA.17330.j.B2.GE37368 FIC 44 275
BaspA.17330.j.B3.GE37369 FIC and OB-Fold 44 333
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: E6YV83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKPYLFKII FEKVIMKKKN LKSLKKIFKP LRKKGEELLD LSSCCDYTYP DSDTLINKHG IKNSQALDEK CAYHSEQAII RLRREALPNK FNSSYLKHIH KCLFEDTFEW AGYTRDLTFK FKNGVTANIS KMQIPNTDAF FKESKAVSKS LHKFDQVLSK KNNLQGLSRE EFVDEATKLF SFLNYIHPFR DGNGRAQRIF FEKLAEAAGH KLDFSVVTQR RMIHACNDAI PVKNNVNYEA MQHLFEDISN PEKRRILKDY LHSVSNNKHA CNFLDDQIII TPREGVTYTG IYKDSNADSI IVETVDLYII CCKDYFTPEQ LKALKPNDKL TFTVPINKDL DQILIPAERL PPLTLEKILR TIENHTELQN NRKEIENLSQ IVYGNSKVLN SSIDVINKGR EVSKKIPEQI LNNPESIAKL AGFKICGIRT PRRRIAEKNL APLSQKIKNY TRSIQITKNI IIRNHVIEQK RLLQEITLPR EIMQDILSVS ESERSKALNV ENCVKTQKEL ISFLSKFESR LSETESQAFR EGDYKQIAQS IGMSEHKAKK LISIVNQVKK VYRQLQQLKV NKSVQMGVAV
NT Sequence
gtgaaaaagc catatttatt taaaataata tttgagaaag tgattatgaa gaagaaaaat ttaaaatctt taaaaaaaat ttttaaacct ttaagaaaaa aaggagaaga attattagac ttgtcttctt gttgtgatta tacttatcct gacagtgata cattaataaa taaacatgga ataaagaatt ctcaagctct tgatgaaaag tgcgcgtatc attcagagca agcaattatt cgtttgcgtc gagaagcact gccaaataag tttaattcct cttatttaaa acatattcat aaatgtttat ttgaagatac tttcgagtgg gctggttata ctcgcgatct tacatttaaa tttaaaaatg gtgtaactgc taacatctca aagatgcaaa taccaaatac agatgctttt tttaaagaga gtaaagcggt ttcaaaaagt ttacataaat ttgatcaagt gctttcgaaa aaaaataatt tacaaggctt atcacgtgaa gaatttgttg atgaagcaac gaagcttttt tcctttctca actatataca tccatttaga gatgggaatg ggcgtgcaca acgaatattt tttgaaaaac tcgcagaggc tgcaggccat aagcttgatt tttcagttgt gactcaaaga cgcatgatac atgcttgcaa tgatgcgata cctgtaaaaa ataatgtaaa ttatgaagca atgcaacatt tgttcgaaga tatctccaat ccagaaaaaa gacgtatttt gaaggattat ttacattctg tgtctaataa taagcatgca tgtaattttt tggatgatca aattattata acaccacgtg aaggtgtgac ctatacaggt atttataagg atagtaatgc ggattctatt atagttgaaa cagtagactt gtatatcata tgctgtaaag attattttac accagaacag cttaaggcat taaaacccaa tgataagctt acttttacag ttccaataaa taaagattta gatcaaattc tcatcccagc agaaagatta cctcctttaa cgttagaaaa aatacttcga acaattgaga atcatactga acttcaaaac aatcgaaaag aaatcgagaa tttatcacaa attgtttatg gaaattcaaa agtattaaat tcaagtattg atgtgattaa taaaggacga gaggtaagta aaaaaattcc tgaacaaatt ttaaataatc ctgaatctat tgctaagctc gctggtttta aaatatgtgg tataaggact ccaagacgca gaatagctga aaagaatctt gcaccactta gtcaaaaaat taaaaattat acgcgttcca tacaaattac taaaaatata atcattagaa atcatgtaat agaacaaaaa cgccttttac aagaaataac actaccccgt gaaataatgc aagatatcct tagcgtgtct gaaagtgaaa gatcaaaagc tttaaatgta gaaaattgtg ttaagaccca aaaagagctt atttcctttt tgtctaaatt tgaatctcgt ctttcagaga cagagtctca agcattcaga gaaggtgatt ataaacaaat cgctcaaagt attggtatgt cagaacataa agcaaaaaaa cttataagta ttgttaatca agtaaaaaaa gtatatcgac aacttcaaca attgaaagtg aataagtcag tacagatggg cgtagctgtt taa
Details for BaspA.17330.j.B1.GE35936
HARVESTED ON: 8/28/2012
SEQUENCED ON: 2/22/2016
EXPECTED MW: 68kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 54
Validated AA Sequence
MAHHHHHHMV KKPYLFKIIF EKVIMKKKNL KSLKKIFKPL RKKGEELLDL SSCCDYTYPD SDTLINKHGI KNSQALDEKC AYHSEQAIIR LRREALPNKF NSSYLKHIHK CLFEDTFEWA GYTRDLTFKF KNGVTANISK MQIPNTDAFF KESKAVSKSL HKFDQVLSKK NNLQGLSREE FVDEATKLFS FLNYIHPFRD GNGRAQRIFF EKLAEAAGHK LDFSVVTQRR MIHACNDAIP VKNNVNYEAM QHLFEDISNP EKRRILKXYL HSVSNNKHAC NFLDDQIIIT XREGVTYTGI YXXXNADSIX XXXXXXXIIC
Validated NT Sequence
atggctcacc accaccacca ccatatggtg aaaaagccat atttatttaa aataatattt gagaaagtga ttatgaagaa gaaaaattta aaatctttaa aaaaaatttt taaaccttta agaaaaaaag gagaagaatt attagacttg tcttcttgtt gtgattatac ttatcctgac agtgatacat taataaataa acatggaata aagaattctc aagctcttga tgaaaagtgc gcgtatcatt cagagcaagc aattattcgt ttgcgtcgag aagcactgcc aaataagttt aattcctctt atttaaaaca tattcataaa tgtttatttg aagatacttt cgagtgggct ggttatactc gcgatcttac atttaaattt aaaaatggtg taactgctaa catctcaaag atgcaaatac caaatacaga tgcttttttt aaagagagta aagcggtttc aaaaagttta cataaatttg atcaagtgct ttcgaaaaaa aataatttac aaggcttatc acgtgaagaa tttgttgatg aagcaacgaa gcttttttcc tttctcaact atatacatcc atttagagat gggaatgggc gtgcacaacg aatatttttt gaaaaactcg cagaggctgc aggccataag cttgattttt cagttgtgac tcaaagacgc atgatacatg cttgcaatga tgcgatacct gtaaaaaata atgtaaatta tgaagcaatg caacatttgt tcgaagatat ctccaatcca gaaaaaagac gtattttgaa ggantattta cattctgtgt ctaataataa gcatgcatgt aattttttgg atgatcaaat tattataacn nnacgtgaag gtgtgaccta tacaggtatt tatnnnnnnn gtaatgcgga ttctattnnn gntgnnnnnn tnnnnntntn tatcatatgc tg
Expected Protein Sequence
MAHHHHHHMV KKPYLFKIIF EKVIMKKKNL KSLKKIFKPL RKKGEELLDL SSCCDYTYPD SDTLINKHGI KNSQALDEKC AYHSEQAIIR LRREALPNKF NSSYLKHIHK CLFEDTFEWA GYTRDLTFKF KNGVTANISK MQIPNTDAFF KESKAVSKSL HKFDQVLSKK NNLQGLSREE FVDEATKLFS FLNYIHPFRD GNGRAQRIFF EKLAEAAGHK LDFSVVTQRR MIHACNDAIP VKNNVNYEAM QHLFEDISNP EKRRILKDYL HSVSNNKHAC NFLDDQIIIT PREGVTYTGI YKDSNADSII VETVDLYIIC CKDYFTPEQL KALKPNDKLT FTVPINKDLD QILIPAERLP PLTLEKILRT IENHTELQNN RKEIENLSQI VYGNSKVLNS SIDVINKGRE VSKKIPEQIL NNPESIAKLA GFKICGIRTP RRRIAEKNLA PLSQKIKNYT RSIQITKNII IRNHVIEQKR LLQEITLPRE IMQDILSVSE SERSKALNVE NCVKTQKELI SFLSKFESRL SETESQAFRE GDYKQIAQSI GMSEHKAKKL ISIVNQVKKV YRQLQQLKVN KSVQMGVAV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga aaaagccata tttatttaaa ataatatttg agaaagtgat tatgaagaag aaaaatttaa aatctttaaa aaaaattttt aaacctttaa gaaaaaaagg agaagaatta ttagacttgt cttcttgttg tgattatact tatcctgaca gtgatacatt aataaataaa catggaataa agaattctca agctcttgat gaaaagtgcg cgtatcattc agagcaagca attattcgtt tgcgtcgaga agcactgcca aataagttta attcctctta tttaaaacat attcataaat gtttatttga agatactttc gagtgggctg gttatactcg cgatcttaca tttaaattta aaaatggtgt aactgctaac atctcaaaga tgcaaatacc aaatacagat gcttttttta aagagagtaa agcggtttca aaaagtttac ataaatttga tcaagtgctt tcgaaaaaaa ataatttaca aggcttatca cgtgaagaat ttgttgatga agcaacgaag cttttttcct ttctcaacta tatacatcca tttagagatg ggaatgggcg tgcacaacga atattttttg aaaaactcgc agaggctgca ggccataagc ttgatttttc agttgtgact caaagacgca tgatacatgc ttgcaatgat gcgatacctg taaaaaataa tgtaaattat gaagcaatgc aacatttgtt cgaagatatc tccaatccag aaaaaagacg tattttgaag gattatttac attctgtgtc taataataag catgcatgta attttttgga tgatcaaatt attataacac cacgtgaagg tgtgacctat acaggtattt ataaggatag taatgcggat tctattatag ttgaaacagt agacttgtat atcatatgct gtaaagatta ttttacacca gaacagctta aggcattaaa acccaatgat aagcttactt ttacagttcc aataaataaa gatttagatc aaattctcat cccagcagaa agattacctc ctttaacgtt agaaaaaata cttcgaacaa ttgagaatca tactgaactt caaaacaatc gaaaagaaat cgagaattta tcacaaattg tttatggaaa ttcaaaagta ttaaattcaa gtattgatgt gattaataaa ggacgagagg taagtaaaaa aattcctgaa caaattttaa ataatcctga atctattgct aagctcgctg gttttaaaat atgtggtata aggactccaa gacgcagaat agctgaaaag aatcttgcac cacttagtca aaaaattaaa aattatacgc gttccataca aattactaaa aatataatca ttagaaatca tgtaatagaa caaaaacgcc ttttacaaga aataacacta ccccgtgaaa taatgcaaga tatccttagc gtgtctgaaa gtgaaagatc aaaagcttta aatgtagaaa attgtgttaa gacccaaaaa gagcttattt cctttttgtc taaatttgaa tctcgtcttt cagagacaga gtctcaagca ttcagagaag gtgattataa acaaatcgct caaagtattg gtatgtcaga acataaagca aaaaaactta taagtattgt taatcaagta aaaaaagtat atcgacaact tcaacaattg aaagtgaata agtcagtaca gatgggcgta gctgtttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BaspA.17330.j.B2.GE37368
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMC CDYTYPDSDT LINKHGIKNS QALDEKCAYH SEQAIIRLRR EALPNKFNSS YLKHIHKCLF EDTFEWAGYT RDLTFKFKNG VTANISKMQI PNTDAFFKES KAVSKSLHKF DQVLSKKNNL QGLSREEFVD EATKLFSFLN YIHPFRDGNG RAQRIFFEKL AEAAGHKLDF SVVTQRRMIH ACNDAIPVKN NVNYEAMQHL FEDISNPEKR RILKDYLHSV SNNKHACNFL D
Validated NT Sequence
atcttactca cttaatccaa aaaattacat gcatgcttat tattagacac agaatgtaaa taatccttca aaatacgtct tttttctgga ttggagatat cttcgaacaa atgttgcatt gcttcataat ttacattatt ttttacaggt atcgcatcat tgcaagcatg tatcatgcgt ctttgagtca caactgaaaa atcaagctta tggcctgcag cctctgcgag tttttcaaaa aatattcgtt gtgcacgccc attcccatct ctaaatggat gtatatagtt gagaaaggaa aaaagcttcg ttgcttcatc aacaaattct tcacgtgata agccttgtaa attatttttt ttcgaaagca cttgatcaaa tttatgtaaa ctttttgaaa ccgctttact ctctttaaaa aaagcatctg tatttggtat ttgcatcttt gagatgttag cagttacacc atttttaaat ttaaatgtaa gatcgcgagt ataaccagcc cactcgaaag tatcttcaaa taaacattta tgaatatgtt ttaaataaga ggaattaaac ttatttggca gtgcttctcg acgcaaacga ataattgctt gctctgaatg atacgcgcac ttttcatcaa gagcttgaga attctttatt ccatgtttat ttattaatgt atcactgtca ggataagtat aatcacaaca catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHCC DYTYPDSDTL INKHGIKNSQ ALDEKCAYHS EQAIIRLRRE ALPNKFNSSY LKHIHKCLFE DTFEWAGYTR DLTFKFKNGV TANISKMQIP NTDAFFKESK AVSKSLHKFD QVLSKKNNLQ GLSREEFVDE ATKLFSFLNY IHPFRDGNGR AQRIFFEKLA EAAGHKLDFS VVTQRRMIHA CNDAIPVKNN VNYEAMQHLF EDISNPEKRR ILKDYLHSVS NNKHACNFLD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattgttgtg attatactta tcctgacagt gatacattaa taaataaaca tggaataaag aattctcaag ctcttgatga aaagtgcgcg tatcattcag agcaagcaat tattcgtttg cgtcgagaag cactgccaaa taagtttaat tcctcttatt taaaacatat tcataaatgt ttatttgaag atactttcga gtgggctggt tatactcgcg atcttacatt taaatttaaa aatggtgtaa ctgctaacat ctcaaagatg caaataccaa atacagatgc tttttttaaa gagagtaaag cggtttcaaa aagtttacat aaatttgatc aagtgctttc gaaaaaaaat aatttacaag gcttatcacg tgaagaattt gttgatgaag caacgaagct tttttccttt ctcaactata tacatccatt tagagatggg aatgggcgtg cacaacgaat attttttgaa aaactcgcag aggctgcagg ccataagctt gatttttcag ttgtgactca aagacgcatg atacatgctt gcaatgatgc gatacctgta aaaaataatg taaattatga agcaatgcaa catttgttcg aagatatctc caatccagaa aaaagacgta ttttgaagga ttatttacat tctgtgtcta ataataagca tgcatgtaat tttttggatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaspA.17330.j.B3.GE37369
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMC CDYTYPDSDT LINKHGIKNS QALDEKCAYH SEQAIIRLRR EALPNKFNSS YLKHIHKCLF EDTFEWAGYT RDLTFKFKNG VTANISKMQI PNTDAFFKES KAVSKSLHKF DQVLSKKNNL QGLSREEFVD EATKLFSFLN YIHPFRDGNG RAQRIFFEKL AEAAGHKLDF SVVTQRRMIH ACNDAIPVKN NVNYEAMQHL FEDISNPEKR RILKDYLHSV SNNKHACNFL DDQIIITPRE GVTYTGIYKD SNADSIIVET VDLYIICCKD YFTPEQLKAL KPNDKLTFT
Validated NT Sequence
tcttactcac ttatgtaaaa gtaagcttat cattgggttt taatgcctta agctgttctg gtgtaaaata atctttacag catatgatat acaagtctac tgtttcaact ataatagaat ccgcattact atccttataa atacctgtat aggtcacacc ttcacgtggt gttataataa tttgatcatc caaaaaatta catgcatgct tattattaga cacagaatgt aaataatcct tcaaaatacg tcttttttct ggattggaga tatcttcgaa caaatgttgc attgcttcat aatttacatt attttttaca ggtatcgcat cattgcaagc atgtatcatg cgtctttgag tcacaactga aaaatcaagc ttatggcctg cagcctctgc gagtttttca aaaaatattc gttgtgcacg cccattccca tctctaaatg gatgtatata gttgagaaag gaaaaaagct tcgttgcttc atcaacaaat tcttcacgtg ataagccttg taaattattt tttttcgaaa gcacttgatc aaatttatgt aaactttttg aaaccgcttt actctcttta aaaaaagcat ctgtatttgg tatttgcatc tttgagatgt tagcagttac accattttta aatttaaatg taagatcgcg agtataacca gcccactcga aagtatcttc aaataaacat ttatgaatat gttttaaata agaggaatta aacttatttg gcagtgcttc tcgacgcaaa cgaataattg cttgctctga atgatacgcg cacttttcat caagagcttg agaattcttt attccatgtt tatttattaa tgtatcactg tcaggataag tataatcaca acacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHCC DYTYPDSDTL INKHGIKNSQ ALDEKCAYHS EQAIIRLRRE ALPNKFNSSY LKHIHKCLFE DTFEWAGYTR DLTFKFKNGV TANISKMQIP NTDAFFKESK AVSKSLHKFD QVLSKKNNLQ GLSREEFVDE ATKLFSFLNY IHPFRDGNGR AQRIFFEKLA EAAGHKLDFS VVTQRRMIHA CNDAIPVKNN VNYEAMQHLF EDISNPEKRR ILKDYLHSVS NNKHACNFLD DQIIITPREG VTYTGIYKDS NADSIIVETV DLYIICCKDY FTPEQLKALK PNDKLTFT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattgttgtg attatactta tcctgacagt gatacattaa taaataaaca tggaataaag aattctcaag ctcttgatga aaagtgcgcg tatcattcag agcaagcaat tattcgtttg cgtcgagaag cactgccaaa taagtttaat tcctcttatt taaaacatat tcataaatgt ttatttgaag atactttcga gtgggctggt tatactcgcg atcttacatt taaatttaaa aatggtgtaa ctgctaacat ctcaaagatg caaataccaa atacagatgc tttttttaaa gagagtaaag cggtttcaaa aagtttacat aaatttgatc aagtgctttc gaaaaaaaat aatttacaag gcttatcacg tgaagaattt gttgatgaag caacgaagct tttttccttt ctcaactata tacatccatt tagagatggg aatgggcgtg cacaacgaat attttttgaa aaactcgcag aggctgcagg ccataagctt gatttttcag ttgtgactca aagacgcatg atacatgctt gcaatgatgc gatacctgta aaaaataatg taaattatga agcaatgcaa catttgttcg aagatatctc caatccagaa aaaagacgta ttttgaagga ttatttacat tctgtgtcta ataataagca tgcatgtaat tttttggatg atcaaattat tataacacca cgtgaaggtg tgacctatac aggtatttat aaggatagta atgcggattc tattatagtt gaaacagtag acttgtatat catatgctgt aaagattatt ttacaccaga acagcttaag gcattaaaac ccaatgataa gcttactttt acatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat