SSGCID: Target Page

BaspA.17330.i

Bep6 protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BaspA.17330.i
ORGANISM:	Bartonella sp. 1-1C
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaspA.17330.i.B3.GE37366	FIC and OB-Fold		2	287
BaspA.17330.i.B4.GE37367	BID		298	436

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaspA.17330.i.B2.PS02003	FIC		2	231

External Resources

RESOURCE	REFERENCE ID
UniProt:	E6YV78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYSGYYYPNT EVLKNKYGIT NKRELNRQCS RETKKIMIAL RQEPSPEEFD SSYLKYLHQR LFESTFEWAG CIRGRTFKFA DGTAAKQYIV KTEDLQDSFL TGYKIEKSFQ EFDKMLSEKD YLRGLSREEF VNEATKLFSF LNYIHPFKDG NRRAQQIFFE KLAEAAGHKL DFSVVTRKRM IHACSQAMPK NANYEAMRHL FEDISNPEKV YLLQEAIYTQ SGKKRKNLDD KIVVIAHEDV AYTGIYRNSE FNSIMIDTVD SYILCCKDYF TPEQLKALKP NDELNFTAPI NKNLNQFLIP AEQLPPLKEE EVIEKIENNA CIQESLQKIR ELSKSVYDNP KILEQDISRI NINPKISIEL SERIINSAKS IGRLKGSKIG RIKSQTYKTS ERNSKMLGNE ISNYGDKVSN IRCTIIRKHR AKERRLLQTV KMPSKKLQDI LNLSVNLQLH ALRSSPETYI EMNDFIRQIT VRLSKKEHQK MCNSNYKELS ESIGISVNKA KMVIETVNKV NKILNILQPK NPQMQFMTL

NT Sequence

atgtattctg gttattatta tcctaatact gaagtattaa aaaacaaata tggaataaca aataaacgag aattaaatag gcaatgttct cgtgagacaa aaaaaataat gattgctctg cgtcaggagc catcgcctga agaatttgat tcttcttatc tgaaatatct tcatcaacgc ttatttgaga gcacattcga atgggctggc tgtattcgag gtcgtacatt taaatttgca gatggcacag ctgccaaaca gtacatagta aaaacagaag atttgcaaga ctcgttttta actggttaca aaatcgaaaa gagctttcaa gaatttgata aaatgctttc tgaaaaagat tatttacgag gcttatcgcg cgaagaattt gtcaatgaag caacaaagct tttttctttt ctcaactata tacatccatt caaagatgga aataggcgtg cgcaacaaat attttttgaa aaacttgcag aagctgcagg ccataagctt gatttttcag ttgtaacccg aaaacgcatg atacatgcct gtagtcaagc gatgcctaaa aatgcaaatt atgaagcgat gcggcattta ttcgaagata tctctaaccc agaaaaagta tatcttttgc aggaagctat ctacacccaa tctgggaaga agcgcaaaaa tctagatgat aaaattgtgg tgatagcaca tgaagatgtt gcctatactg gtatttacag aaacagtgaa tttaattcta ttatgataga cacagtggat tcatacatct tatgctgtaa agattatttt acaccagaac aacttaaggc attaaaacct aatgatgagc ttaactttac agctccaata aataaaaatt taaatcaatt tctcatccca gcagaacaat tacccccttt aaaggaagaa gaagtaattg aaaagattga aaataatgct tgtattcaag aaagcttaca aaaaattaga gagttatcaa aatctgttta cgataatccc aaaatactag aacaagatat aagtcgtatt aatataaatc ctaaaataag tatagagctt tctgaacgga ttataaattc tgctaaatct attggtcggc taaagggtag taaaattggg cgcatcaaaa gtcaaacata taaaacttct gaacgtaatt ctaaaatgct tggtaatgaa atatctaatt atggagataa agtatcaaat attagatgta caattattag aaaacataga gcaaaagaaa gacgtctttt acaaacagta aagatgccta gcaaaaaatt acaagatata cttaatttgt ctgtaaattt gcaactacat gctttacgtt cctctcctga aacttatata gaaatgaatg atttcatacg tcaaattact gtgcgtttat caaaaaaaga acatcaaaag atgtgtaatt ctaattataa agaactgtct gaaagtattg gcatatcagt caataaggcg aaaatggtta tagaaactgt taataaagtg aataaaattt tgaacatatt gcaacctaaa aatccacaaa tgcaatttat gactctctga

Details for BaspA.17330.i.B3.GE37366

HARVESTED ON:	9/27/2013
SEQUENCED ON:	10/3/2013
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMY SGYYYPNTEV LKNKYGITNK RELNRQCSRE TKKIMIALRQ EPSPEEFDSS YLKYLHQRLF ESTFEWAGCI RGRTFKFADG TAAKQYIVKT EDLQDSFLTG YKIEKSFQEF DKMLSEKDYL RGLSREEFVN EATKLFSFLN YIHPFKDGNR RAQQIFFEKL AEAAGHKLDF SVVTRKRMIH ACSQAMPKNA NYEAMRHLFE DISNPEKVYL LQEAIYTQSG KKRKNLDDKI VVIAHEDVAY TGIYRNSEFN SIMIDTVDSY ILCCKDYFTP EQLKALKPND ELNFT

Validated NT Sequence

tcttactcac ttatgtaaag ttaagctcat cattaggttt taatgcctta agttgttctg gtgtaaaata atctttacag cataagatgt atgaatccac tgtgtctatc ataatagaat taaattcact gtttctgtaa ataccagtat aggcaacatc ttcatgtgct atcaccacaa ttttatcatc tagatttttg cgcttcttcc cagattgggt gtagatagct tcctgcaaaa gatatacttt ttctgggtta gagatatctt cgaataaatg ccgcatcgct tcataatttg catttttagg catcgcttga ctacaggcat gtatcatgcg ttttcgggtt acaactgaaa aatcaagctt atggcctgca gcttctgcaa gtttttcaaa aaatatttgt tgcgcacgcc tatttccatc tttgaatgga tgtatatagt tgagaaaaga aaaaagcttt gttgcttcat tgacaaattc ttcgcgcgat aagcctcgta aataatcttt ttcagaaagc attttatcaa attcttgaaa gctcttttcg attttgtaac cagttaaaaa cgagtcttgc aaatcttctg tttttactat gtactgtttg gcagctgtgc catctgcaaa tttaaatgta cgacctcgaa tacagccagc ccattcgaat gtgctctcaa ataagcgttg atgaagatat ttcagataag aagaatcaaa ttcttcaggc gatggctcct gacgcagagc aatcattatt ttttttgtct cacgagaaca ttgcctattt aattctcgtt tatttgttat tccatatttg ttttttaata cttcagtatt aggataataa taaccagaat acatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHYS GYYYPNTEVL KNKYGITNKR ELNRQCSRET KKIMIALRQE PSPEEFDSSY LKYLHQRLFE STFEWAGCIR GRTFKFADGT AAKQYIVKTE DLQDSFLTGY KIEKSFQEFD KMLSEKDYLR GLSREEFVNE ATKLFSFLNY IHPFKDGNRR AQQIFFEKLA EAAGHKLDFS VVTRKRMIHA CSQAMPKNAN YEAMRHLFED ISNPEKVYLL QEAIYTQSGK KRKNLDDKIV VIAHEDVAYT GIYRNSEFNS IMIDTVDSYI LCCKDYFTPE QLKALKPNDE LNFT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattattctg gttattatta tcctaatact gaagtattaa aaaacaaata tggaataaca aataaacgag aattaaatag gcaatgttct cgtgagacaa aaaaaataat gattgctctg cgtcaggagc catcgcctga agaatttgat tcttcttatc tgaaatatct tcatcaacgc ttatttgaga gcacattcga atgggctggc tgtattcgag gtcgtacatt taaatttgca gatggcacag ctgccaaaca gtacatagta aaaacagaag atttgcaaga ctcgttttta actggttaca aaatcgaaaa gagctttcaa gaatttgata aaatgctttc tgaaaaagat tatttacgag gcttatcgcg cgaagaattt gtcaatgaag caacaaagct tttttctttt ctcaactata tacatccatt caaagatgga aataggcgtg cgcaacaaat attttttgaa aaacttgcag aagctgcagg ccataagctt gatttttcag ttgtaacccg aaaacgcatg atacatgcct gtagtcaagc gatgcctaaa aatgcaaatt atgaagcgat gcggcattta ttcgaagata tctctaaccc agaaaaagta tatcttttgc aggaagctat ctacacccaa tctgggaaga agcgcaaaaa tctagatgat aaaattgtgg tgatagcaca tgaagatgtt gcctatactg gtatttacag aaacagtgaa tttaattcta ttatgataga cacagtggat tcatacatct tatgctgtaa agattatttt acaccagaac aacttaaggc attaaaacct aatgatgagc ttaactttac atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BaspA.17330.i.B4.GE37367

HARVESTED ON:	9/27/2013
SEQUENCED ON:	10/3/2013
EXPECTED MW:	17kDa
OBSERVED MW:	17kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML IPAEQLPPLK EEEVIEKIEN NACIQESLQK IRELSKSVYD NPKILEQDIS RININPKISI ELSERIINSA KSIGRLKGSK IGRIKSQTYK TSERNSKMLG NEISNYGDKV SNIRCTIIRK HRAKERRLLQ TVKMPSKK

Validated NT Sequence

anannanttt ntttaacttt aagaaggaga nataccatgg ctcaccacca ccaccaccat atgctcatcc cagcagaaca attaccccct ttaaaggaag aagaagtaat tgaaaagatt gaaaataatg cttgtattca agaaagctta caaaaaatta gagagttatc aaaatctgtt tacgataatc ccaaaatact agaacaagat ataagtcgta ttaatataaa tcctaaaata agtatagagc tttctgaacg gattataaat tctgctaaat ctattggtcg gctaaagggt agtaaaattg ggcgcatcaa aagtcaaaca tataaaactt ctgaacgtaa ttctaaaatg cttggtaatg aaatatctaa ttatggagat aaagtatcaa atattagatg tacaattatt agaaaacata gagcaaaaga aagacgtctt ttacaaacag taaagatgcc tagcaaaaaa taagtgagta agataggatc cggctgctaa caanccnccg aaagannnnn nnnttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn anctnncnnn nncnnnngnn acnannnncn nnngcncnnn nannnnnnnn nnacnannng nnnnnnnnnn nnnnnnnnnc nnnnnnnnnt atnnangnta canganncgn nnnnnnnncg ngnnnncntc nnatnnnnnn nnnnnnntnn annnangcnn nnnnnacngt nnnnntgcan nnnnnntnna cnnnnnncnn gtcnnnnnnn nntnnncnna nnnnnnnnnn nnnnngannn tnnnnnannt nnnngnnnnn nngatcatnn nnnnnnnnnn nnnnnnngac ntnnntnnnn ncannannnn nnnnnnannn nnnnnnnnta anngcganna nnnnnnnagn nnnnnnnnnn nnnnnnntnn nntnnng

Expected Protein Sequence

MAHHHHHHLI PAEQLPPLKE EEVIEKIENN ACIQESLQKI RELSKSVYDN PKILEQDISR ININPKISIE LSERIINSAK SIGRLKGSKI GRIKSQTYKT SERNSKMLGN EISNYGDKVS NIRCTIIRKH RAKERRLLQT VKMPSKK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctcatcc cagcagaaca attaccccct ttaaaggaag aagaagtaat tgaaaagatt gaaaataatg cttgtattca agaaagctta caaaaaatta gagagttatc aaaatctgtt tacgataatc ccaaaatact agaacaagat ataagtcgta ttaatataaa tcctaaaata agtatagagc tttctgaacg gattataaat tctgctaaat ctattggtcg gctaaagggt agtaaaattg ggcgcatcaa aagtcaaaca tataaaactt ctgaacgtaa ttctaaaatg cttggtaatg aaatatctaa ttatggagat aaagtatcaa atattagatg tacaattatt agaaaacata gagcaaaaga aagacgtctt ttacaaacag taaagatgcc tagcaaaaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for BaspA.17330.i.B2.PS02003

PURIFICATION DATe:	2/20/2014
CONCENTRATION:	11.7mg/ml
OBSERVED MW:	27kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

atggctcacc accaccacca ccatatgtat tctggttatt attatcctaa tactgaagta ttaaaaaaca aatatggaat aacaaataaa cgagaattaa ataggcaatg ttctcgtgag acaaaaaaaa taatgattgc tctgcgtcag gagccatcgc ctgaagaatt tgattcttct tatctgaaat atcttcatca acgcttattt gagagcacat tcgaatgggc tggctgtatt cgaggtcgta catttaaatt tgcagatggc acagctgcca aacagtacat agtaaaaaca gaagatttgc aagactcgtt tttaactggt tacaaaatcg aaaagagctt tcaagaattt gataaaatgc tttctgaaaa agattattta cgaggcttat cgcgcgaaga atttgtcaat gaagcaacaa agcttttttc ttttctcaac tatatacatc cattcaaaga tggaaatagg cgtgcgcaac aaatattttt tgaaaaactt gcagaagctg caggccataa gcttgatttt tcagttgtaa cccgaaaacg catgatacat gcctgtagtc aagcgatgcc taaaaatgca aattatgaag cgatgcggca tttattcgaa gatatctcta acccagaaaa agtatatctt ttgcaggaag ctatctacac ccaatctggg aagaagcgca aaaatctaga tgataaataa gtgagtaaga t

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTATTCTG GTTATTATTA TCCTAATACT GAAGTATTAA AAAACAAATA TGGAATAACA AATAAACGAG AATTAAATAG GCAATGTTCT CGTGAGACAA AAAAAATAAT GATTGCTCTG CGTCAGGAGC CATCGCCTGA AGAATTTGAT TCTTCTTATC TGAAATATCT TCATCAACGC TTATTTGAGA GCACATTCGA ATGGGCTGGC TGTATTCGAG GTCGTACATT TAAATTTGCA GATGGCACAG CTGCCAAACA GTACATAGTA AAAACAGAAG ATTTGCAAGA CTCGTTTTTA ACTGGTTACA AAATCGAAAA GAGCTTTCAA GAATTTGATA AAATGCTTTC TGAAAAAGAT TATTTACGAG GCTTATCGCG CGAAGAATTT GTCAATGAAG CAACAAAGCT TTTTTCTTTT CTCAACTATA TACATCCATT CAAAGATGGA AATAGGCGTG CGCAACAAAT ATTTTTTGAA AAACTTGCAG AAGCTGCAGG CCATAAGCTT GATTTTTCAG TTGTAACCCG AAAACGCATG ATACATGCCT GTAGTCAAGC GATGCCTAAA AATGCAAATT ATGAAGCGAT GCGGCATTTA TTCGAAGATA TCTCTAACCC AGAAAAAGTA TATCTTTTGC AGGAAGCTAT CTACACCCAA TCTGGGAAGA AGCGCAAAAA TCTAGATGAT AAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT