BaspA.17330.h

Bep2 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaspA.17330.h
ORGANISM: Bartonella sp. 1-1C
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaspA.17330.h.B1.GE35935 full length 1 569
BaspA.17330.h.B2.GE37362 FIC 46 274
BaspA.17330.h.B3.GE37363 FIC and OB-Fold 46 330
BaspA.17330.h.B4.GE37364 BID 341 470
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: E6YW78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYFIILKDLK FYEDIWGYFI NLNYQGVVMK KSEMMITLGD INVPSPKNYV YPDSVVLKNK YGIMDLREFE ERMAHDTSKE TVNILEEPAP ERFDSSYLKY LHKRLFQHAL EWAGCTRDVP FTLSDGTNVV MPSMAKSDSD FGFAFGPQIK EGLDFIDQKL AEKNYLRGLS REEFVHDAAR IFGSINYIHP FREGNGRVQR LFFVKLARSV GYKLDFSATT TKRMTYVSVE VTKNAYMEPL QHLFEDISNP EKARILKQFI RKAKEDNIFD ASEKLITVAR EGETYKGIGK FLASDFIVIE AGDTCILSNK DSIAPEQLRT FKLGSDFIFT ALDTKELKEI FIPEEKLAAL REDEMIEKIL ECSSVREQRD KIQHCARFIY GRSDKLNEII NMIDMDPNLG EIFSEQIMAC CDNKKYFINK KVKTTNESVC KLSNEIKNYA NIVEDSRNQI LKEYKERQKR CGQVVKMPSK EVQCILDMPE DMREESLKSR SLSFLNESIS DFIIKVNSRL SPRECKAIND NNYEELAKSI SVSESKAKAI IKTIGEVKEI QKKIRVLRAN ALKVPAIAC
NT Sequence
gtgtatttta ttatcttaaa agatttaaaa ttttatgaag atatatgggg gtattttata aatctaaatt atcaaggtgt tgttatgaag aaaagtgaaa tgatgataac gttgggggat attaacgtac cttcgcctaa gaattatgtc tatcctgaca gtgtagtgtt gaagaataaa tatgggatta tggatcttcg agagttcgag gagagaatgg cgcatgatac atcaaaggaa actgttaata ttctggaaga acctgctcca gagcggtttg attcttctta tctaaagtat ttgcacaagc gtttatttca gcatgcatta gaatgggctg ggtgcacccg tgatgttcca tttacacttt cagatggtac aaacgttgtg atgccctcca tggcaaagtc tgattccgat tttggttttg catttggtcc acagattaag gaaggtttag atttcatcga tcagaaacta gctgagaaga attatttacg aggcttgtcg cgtgaagaat ttgttcatga tgcagcaaga atatttggtt ctatcaatta tattcaccct tttagggaag gcaatggacg cgtacaaaga ttgttttttg taaagcttgc tagatctgtc ggctataaac ttgacttttc cgccacaaca acaaagcgca tgacatatgt gagtgttgag gttacaaaaa acgcgtatat ggagcctttg caacatttat ttgaagatat ttccaatcca gaaaaggcgc gtattctaaa gcaattcata agaaaggcaa aggaagataa tatctttgat gcgagtgaaa agttgattac agtagcgaga gaaggtgaaa cctataaggg gattggtaaa tttcttgctt cagattttat tgtaatagaa gcgggagata cttgtatcct ctctaataaa gattccattg caccagaaca attaaggaca ttcaaacttg gtagtgattt catttttaca gctttagata ctaaagagct taaagaaatt tttatcccag aagaaaaatt agctgctttg agggaagatg aaatgattga aaaaattttg gaatgtagtt ctgttcgaga gcaacgagat aaaatccagc attgtgcaag atttatttat ggacgttcag ataaattgaa cgaaataata aacatgattg atatggatcc aaatttgggg gagatatttt ctgaacagat tatggcatgt tgtgataaca aaaagtattt cataaataaa aaagttaaaa ctactaatga aagtgtttgt aaacttagta atgaaattaa aaattatgca aatattgtag aagattctag aaatcaaatc ttaaaggagt ataaagaaag acaaaagcgt tgtggacagg tggtaaaaat gcctagcaag gaagtgcaat gcattctcga tatgccagaa gatatgcggg aagaatcttt gaaatcacgg agtctttcat ttcttaatga aagtatctct gattttatca ttaaagttaa ttctcgcctt tcaccacgtg aatgtaaggc aatcaatgat aataactatg aagaactcgc taaaagtatt agcgtatcag aaagtaaagc aaaggcaatt ataaaaacta ttggggaagt caaagaaata caaaagaaaa tacgagtact cagagcgaat gctttaaagg tgccagctat agcttgttaa
Details for BaspA.17330.h.B1.GE35935
HARVESTED ON: 8/28/2012
SEQUENCED ON: 2/22/2016
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 52
Validated AA Sequence
MAHHHHHHMV YFIILKDLKF YEDIWGYFIN LNYQGVVMKK SEMMITLGDI NVPSPKNYVY PDSVVLKNKY GIMDLREFEE RMAHDTSKET VNILEEPAPE RFDSSYLKYL HKRLFQHALE WAGCTRDVPF TLSDGTNVVM PSMAKSDSDF GFAFGPQIKE GLDFIDQKLA EKNYLRGLSR EEFVHDAARI FGSINYIHPF REGNGRVQRL FFVKLARSVG YKLDFSATTT KRMTYVSVEV TKNAYMEPLQ HLFEDISXPE KARIXXXFIR KAXXXXIFDA XXXXITVXXX XETYXXXXXX X
Validated NT Sequence
atggctcacc accaccacca ccatatggtg tattttatta tcttaaaaga tttaaaattt tatgaagata tatgggggta ttttataaat ctaaattatc aaggtgttgt tatgaagaaa agtgaaatga tgataacgtt gggggatatt aacgtacctt cgcctaagaa ttatgtctat cctgacagtg tagtgttgaa gaataaatat gggattatgg atcttcgaga gttcgaggag agaatggcgc atgatacatc aaaggaaact gttaatattc tggaagaacc tgctccagag cggtttgatt cttcttatct aaagtatttg cacaagcgtt tatttcagca tgcattagaa tgggctgggt gcacccgtga tgttccattt acactttcag atggtacaaa cgttgtgatg ccctccatgg caaagtctga ttccgatttt ggttttgcat ttggtccaca gattaaggaa ggtttagatt tcatcgatca gaaactagct gagaagaatt atttacgagg cttgtcgcgt gaagaatttg ttcatgatgc agcaagaata tttggttcta tcaattatat tcaccctttt agggaaggca atggacgcgt acaaagattg ttttttgtaa agcttgctag atctgtcggc tataaacttg acttttccgc cacaacaaca aagcgcatga catatgtgag tgttgaggtt acaaaaaacg cgtatatgga gcctttgcaa catttatttg aagatatttc nnntccagaa aaggcgcgta ttcnnnngna attcataaga aaggcannnn nngntnatat ctttgatgcg ngnnnnnagn tgattacagt nnnnnnnnnn nntgaaacct atnnngngat nnntnnnnnn cntgc
Expected Protein Sequence
MAHHHHHHMV YFIILKDLKF YEDIWGYFIN LNYQGVVMKK SEMMITLGDI NVPSPKNYVY PDSVVLKNKY GIMDLREFEE RMAHDTSKET VNILEEPAPE RFDSSYLKYL HKRLFQHALE WAGCTRDVPF TLSDGTNVVM PSMAKSDSDF GFAFGPQIKE GLDFIDQKLA EKNYLRGLSR EEFVHDAARI FGSINYIHPF REGNGRVQRL FFVKLARSVG YKLDFSATTT KRMTYVSVEV TKNAYMEPLQ HLFEDISNPE KARILKQFIR KAKEDNIFDA SEKLITVARE GETYKGIGKF LASDFIVIEA GDTCILSNKD SIAPEQLRTF KLGSDFIFTA LDTKELKEIF IPEEKLAALR EDEMIEKILE CSSVREQRDK IQHCARFIYG RSDKLNEIIN MIDMDPNLGE IFSEQIMACC DNKKYFINKK VKTTNESVCK LSNEIKNYAN IVEDSRNQIL KEYKERQKRC GQVVKMPSKE VQCILDMPED MREESLKSRS LSFLNESISD FIIKVNSRLS PRECKAINDN NYEELAKSIS VSESKAKAII KTIGEVKEIQ KKIRVLRANA LKVPAIAC
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgt attttattat cttaaaagat ttaaaatttt atgaagatat atgggggtat tttataaatc taaattatca aggtgttgtt atgaagaaaa gtgaaatgat gataacgttg ggggatatta acgtaccttc gcctaagaat tatgtctatc ctgacagtgt agtgttgaag aataaatatg ggattatgga tcttcgagag ttcgaggaga gaatggcgca tgatacatca aaggaaactg ttaatattct ggaagaacct gctccagagc ggtttgattc ttcttatcta aagtatttgc acaagcgttt atttcagcat gcattagaat gggctgggtg cacccgtgat gttccattta cactttcaga tggtacaaac gttgtgatgc cctccatggc aaagtctgat tccgattttg gttttgcatt tggtccacag attaaggaag gtttagattt catcgatcag aaactagctg agaagaatta tttacgaggc ttgtcgcgtg aagaatttgt tcatgatgca gcaagaatat ttggttctat caattatatt caccctttta gggaaggcaa tggacgcgta caaagattgt tttttgtaaa gcttgctaga tctgtcggct ataaacttga cttttccgcc acaacaacaa agcgcatgac atatgtgagt gttgaggtta caaaaaacgc gtatatggag cctttgcaac atttatttga agatatttcc aatccagaaa aggcgcgtat tctaaagcaa ttcataagaa aggcaaagga agataatatc tttgatgcga gtgaaaagtt gattacagta gcgagagaag gtgaaaccta taaggggatt ggtaaatttc ttgcttcaga ttttattgta atagaagcgg gagatacttg tatcctctct aataaagatt ccattgcacc agaacaatta aggacattca aacttggtag tgatttcatt tttacagctt tagatactaa agagcttaaa gaaattttta tcccagaaga aaaattagct gctttgaggg aagatgaaat gattgaaaaa attttggaat gtagttctgt tcgagagcaa cgagataaaa tccagcattg tgcaagattt atttatggac gttcagataa attgaacgaa ataataaaca tgattgatat ggatccaaat ttgggggaga tattttctga acagattatg gcatgttgtg ataacaaaaa gtatttcata aataaaaaag ttaaaactac taatgaaagt gtttgtaaac ttagtaatga aattaaaaat tatgcaaata ttgtagaaga ttctagaaat caaatcttaa aggagtataa agaaagacaa aagcgttgtg gacaggtggt aaaaatgcct agcaaggaag tgcaatgcat tctcgatatg ccagaagata tgcgggaaga atctttgaaa tcacggagtc tttcatttct taatgaaagt atctctgatt ttatcattaa agttaattct cgcctttcac cacgtgaatg taaggcaatc aatgataata actatgaaga actcgctaaa agtattagcg tatcagaaag taaagcaaag gcaattataa aaactattgg ggaagtcaaa gaaatacaaa agaaaatacg agtactcaga gcgaatgctt taaaggtgcc agctatagct tgttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BaspA.17330.h.B2.GE37362
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP KNYVYPDSVV LKNKYGIMDL REFEERMAHD TSKETVNILE EPAPERFDSS YLKYLHKRLF QHALEWAGCT RDVPFTLSDG TNVVMPSMAK SDSDFGFAFG PQIKEGLDFI DQKLAEKNYL RGLSREEFVH DAARIFGSIN YIHPFREGNG RVQRLFFVKL ARSVGYKLDF SATTTKRMTY VSVEVTKNAY MEPLQHLFED ISNPEKARIL KQFIRKAKED NIFERVKSK
Validated NT Sequence
tttntttnac tttaagaagg aganatacca tggctcacca ccaccaccac catatgccta agaattatgt ctatcctgac agtgtagtgt tgaagaataa atatgggatt atggatcttc gagagttcga ggagagaatg gcgcatgata catcaaagga aactgttaat attctggaag aacctgctcc agagcggttt gattcttctt atctaaagta tttgcacaag cgtttatttc agcatgcatt agaatgggct gggtgcaccc gtgatgttcc atttacactt tcagatggta caaacgttgt gatgccctcc atggcaaagt ctgattccga ttttggtttt gcatttggtc cacagattaa ggaaggttta gatttcatcg atcagaaact agctgagaag aattatttac gaggcttgtc gcgtgaagaa tttgttcatg atgcagcaag aatatttggt tctatcaatt atattcaccc ttttagggaa ggcaatggac gcgtacaaag attgtttttt gtaaagcttg ctagatctgt cggctataaa cttgactttt ccgccacaac aacaaagcgc atgacatatg tgagtgttga ggttacaaaa aacgcgtata tggagccttt gcaacattta tttgaagata tttccaatcc agaaaaggcg cgtattctaa agcaattcat aagaaaggca aaggaagata atatctttga gcgagtgaaa agtaagtgag taaganagga tccgnnnnnn nnnnnccccc nnanannnnn nnnnnnnnna aaanannnnn nnnnnnnncn gnnnngntcc nnnnnannnn anccnntgnn nnnnnnanna nnnnnnnnnn ttgnnnngat ccnnnnnnnn nnnnnntnnn nnnannnnnn nnnnntnggn nnnnnnngan ctnnnnnnnn nnt
Expected Protein Sequence
MAHHHHHHPK NYVYPDSVVL KNKYGIMDLR EFEERMAHDT SKETVNILEE PAPERFDSSY LKYLHKRLFQ HALEWAGCTR DVPFTLSDGT NVVMPSMAKS DSDFGFAFGP QIKEGLDFID QKLAEKNYLR GLSREEFVHD AARIFGSINY IHPFREGNGR VQRLFFVKLA RSVGYKLDFS ATTTKRMTYV SVEVTKNAYM EPLQHLFEDI SNPEKARILK QFIRKAKEDN IFDASEK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcctaaga attatgtcta tcctgacagt gtagtgttga agaataaata tgggattatg gatcttcgag agttcgagga gagaatggcg catgatacat caaaggaaac tgttaatatt ctggaagaac ctgctccaga gcggtttgat tcttcttatc taaagtattt gcacaagcgt ttatttcagc atgcattaga atgggctggg tgcacccgtg atgttccatt tacactttca gatggtacaa acgttgtgat gccctccatg gcaaagtctg attccgattt tggttttgca tttggtccac agattaagga aggtttagat ttcatcgatc agaaactagc tgagaagaat tatttacgag gcttgtcgcg tgaagaattt gttcatgatg cagcaagaat atttggttct atcaattata ttcacccttt tagggaaggc aatggacgcg tacaaagatt gttttttgta aagcttgcta gatctgtcgg ctataaactt gacttttccg ccacaacaac aaagcgcatg acatatgtga gtgttgaggt tacaaaaaac gcgtatatgg agcctttgca acatttattt gaagatattt ccaatccaga aaaggcgcgt attctaaagc aattcataag aaaggcaaag gaagataata tctttgatgc gagtgaaaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BaspA.17330.h.B3.GE37363
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP KNYVYPDSVV LKNKYGIMDL REFEERMAHD TSKETVNILE EPAPERFDSS YLKYLHKRLF QHALEWAGCT RDVPFTLSDG TNVVMPSMAK SDSDFGFAFG PQIKEGLDFI DQKLAEKNYL RGLSREEFVH DAARIFGSIN YIHPFREGNG RVQRLFFVKL ARSVGYKLDF SATTTKRMTY VSVEVTKNAY MEPLQHLFED ISNPEKARIL KQFIRKAKED NIFDASEKLI TVAREGETYX GIGKFLASDF IVIEAGDTCI LSNKXSIAPE QLRTFKLGSD FIFT
Validated NT Sequence
ttttntttna ctttaagang gagatatacc atggctcacc accaccacca ccatatgcct aagaattatg tctatcctga cagtgtagtg ttgaagaata aatatgggat tatggatctt cgagagttcg aggagagaat ggcgcatgat acatcaaagg aaactgttaa tattctggaa gaacctgctc cagagcggtt tgattcttct tatctaaagt atttgcacaa gcgtttattt cagcatgcat tagaatgggc tgggtgcacc cgtgatgttc catttacact ttcagatggt acaaacgttg tgatgccctc catggcaaag tctgattccg attttggttt tgcatttggt ccacagatta aggaaggttt agatttcatc gatcagaaac tagctgagaa gaattattta cgaggcttgt cgcgtgaaga atttgttcat gatgcagcaa gaatatttgg ttctatcaat tatattcacc cttttaggga aggcaatgga cgcgtacaaa gattgttttt tgtaaagctt gctagatctg tcggctataa acttgacttt tccgccacaa caacaaagcg catgacatat gtgagtgttg aggttacaaa aaacgcgtat atggagcctt tgcaacattt atttgaagat atttccaatc cagaaaaggc gcgtattcta aagcaattca taagaaaggc aaaggaagat aatatctttg atgcgagtga aaagttgatt acagtagcga gagaaggtga aacctataan gggattggta aatttcttgc ttcagatttt attgtaatag aagcgggaga tacttgtatc ctctctaata aagantccat tgcaccagaa caattaagga cattcaaact tggtagtgat ttcattttta cataantgag taananagna nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncnnnn naaaaaaana nnnnnnnnnn ntnngannnn nnnnnnnnnn nnnnntngnn nnnt
Expected Protein Sequence
MAHHHHHHPK NYVYPDSVVL KNKYGIMDLR EFEERMAHDT SKETVNILEE PAPERFDSSY LKYLHKRLFQ HALEWAGCTR DVPFTLSDGT NVVMPSMAKS DSDFGFAFGP QIKEGLDFID QKLAEKNYLR GLSREEFVHD AARIFGSINY IHPFREGNGR VQRLFFVKLA RSVGYKLDFS ATTTKRMTYV SVEVTKNAYM EPLQHLFEDI SNPEKARILK QFIRKAKEDN IFDASEKLIT VAREGETYKG IGKFLASDFI VIEAGDTCIL SNKDSIAPEQ LRTFKLGSDF IFT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcctaaga attatgtcta tcctgacagt gtagtgttga agaataaata tgggattatg gatcttcgag agttcgagga gagaatggcg catgatacat caaaggaaac tgttaatatt ctggaagaac ctgctccaga gcggtttgat tcttcttatc taaagtattt gcacaagcgt ttatttcagc atgcattaga atgggctggg tgcacccgtg atgttccatt tacactttca gatggtacaa acgttgtgat gccctccatg gcaaagtctg attccgattt tggttttgca tttggtccac agattaagga aggtttagat ttcatcgatc agaaactagc tgagaagaat tatttacgag gcttgtcgcg tgaagaattt gttcatgatg cagcaagaat atttggttct atcaattata ttcacccttt tagggaaggc aatggacgcg tacaaagatt gttttttgta aagcttgcta gatctgtcgg ctataaactt gacttttccg ccacaacaac aaagcgcatg acatatgtga gtgttgaggt tacaaaaaac gcgtatatgg agcctttgca acatttattt gaagatattt ccaatccaga aaaggcgcgt attctaaagc aattcataag aaaggcaaag gaagataata tctttgatgc gagtgaaaag ttgattacag tagcgagaga aggtgaaacc tataagggga ttggtaaatt tcttgcttca gattttattg taatagaagc gggagatact tgtatcctct ctaataaaga ttccattgca ccagaacaat taaggacatt caaacttggt agtgatttca tttttacatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BaspA.17330.h.B4.GE37364
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 16kDa
OBSERVED MW: 16kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMF IPEEKLAALR EDEMIEKILE CSSVREQRDK IQHCARFIYG RSDKLNEIIN MIDMDPNLGE IFSEQIMACC DNKKYFINKK VKTTNESVCK LSNEIKNYAN IVEDSRNQIL KEYKERQKRC GQVVKMPSK
Validated NT Sequence
tgttagcagc cggatcctat cttactcact tacttgctag gcatttttac cacctgtcca caacgctttt gtctttcttt atactccttt aagatttgat ttctagaatc ttctacaata tttgcataat ttttaatttc attactaagt ttacaaacac tttcattagt agttttaact tttttattta tgaaatactt tttgttatca caacatgcca taatctgttc agaaaatatc tcccccaaat ttggatccat atcaatcatg tttattattt cgttcaattt atctgaacgt ccataaataa atcttgcaca atgctggatt ttatctcgtt gctctcgaac agaactacat tccaaaattt tttcaatcat ttcatcttcc ctcaaagcag ctaatttttc ttctgggata aacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHFI PEEKLAALRE DEMIEKILEC SSVREQRDKI QHCARFIYGR SDKLNEIINM IDMDPNLGEI FSEQIMACCD NKKYFINKKV KTTNESVCKL SNEIKNYANI VEDSRNQILK EYKERQKRCG QVVKMPSK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattttatcc cagaagaaaa attagctgct ttgagggaag atgaaatgat tgaaaaaatt ttggaatgta gttctgttcg agagcaacga gataaaatcc agcattgtgc aagatttatt tatggacgtt cagataaatt gaacgaaata ataaacatga ttgatatgga tccaaatttg ggggagatat tttctgaaca gattatggca tgttgtgata acaaaaagta tttcataaat aaaaaagtta aaactactaa tgaaagtgtt tgtaaactta gtaatgaaat taaaaattat gcaaatattg tagaagattc tagaaatcaa atcttaaagg agtataaaga aagacaaaag cgttgtggac aggtggtaaa aatgcctagc aagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat