BaspA.17330.g

Bep5 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaspA.17330.g
ORGANISM: Bartonella sp. 1-1C
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaspA.17330.g.B1.GE35713 full length 1 511
BaspA.17330.g.B3.GE37058 partial FIC and OB-Fold 1 264
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaspA.17330.g.B1.PW36353 full length 1 511

External Resources

RESOURCE REFERENCE ID
UniProt: E6YVB0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQGIEFRNVC AYDVLKAEYR LKKEPLPEKF DSSYLKYLHK CLFEETFEWA GHTRDIPFTF LDGSVTMVPV NDKIKDGLER IDQILAEKNN LKGLSRKEFV YEASMLFALF SNLRPFAVGN GCAQRIFMEK IAEAAGHRLD FSVVTEQRMR SANNAALPHD GKAVGNITPM CHLFEDISNP EKVDVLREFM NAIRNRGSFD EYANIRNKII VAPNEGSTYT GIYKDSSPDS IMLMVNDKYV ICKKDYLAPE ILTALRLGDK LTFRASVPEN FEHILIPEEK VASLTEKEIV AKIVGNVFVQ KSQQKIKTLS KSVYKNSKML DADINLVNID SNLGEQYIRQ NISPFKYMSK LSGTEILGIN SPQRKQAKKD YFNLSQEFDN YISVVLSTRE QILQVHQEKQ ERCQQSIKMP SQAVQNVLSL SEEDQIKALN ANPSLHEEIT GLLDKVKDRL TFWEYQALDD RDYEKLSKSI GISVSRAQKV TEIIKKAQKV CQLTRSRAHE LKAMECNRLQ I
NT Sequence
ttgcaaggca tagagtttcg aaatgtatgt gcctatgatg tactaaaagc agagtacagg ctaaaaaaag aaccgctacc agaaaagttt gattcgtctt atctaaaata tcttcataaa tgtttatttg aagagacgtt tgaatgggct gggcataccc gtgacattcc atttacattt ttagatggct cagttaccat ggtgccagtc aatgataaaa ttaaagacgg tttagagaga atcgaccaaa tactagccga aaagaataat ttgaaaggtt tatcacgtaa agagtttgtc tatgaagcat cgatgctctt tgctttgttt agtaatttac gacctttcgc ggttggaaat gggtgcgcac aacgaatatt tatggaaaaa atagccgaag cagcggggca taggcttgac ttctcggtcg tgacagaaca acgcatgcga tctgctaata atgctgcgtt accacatgat ggcaaagctg taggcaatat cactcctatg tgtcatctgt ttgaagatat ctccaatcca gaaaaagtgg atgttttaag agaattcatg aatgccataa ggaatcgggg atcttttgat gagtacgcaa atataagaaa taagattatt gtagcgccaa atgaaggttc tacttataca ggcatctata aagatagtag cccagattct attatgctca tggtgaatga taagtatgtt atatgcaaaa aagattatct tgcaccagaa atattaacag cattacggct tggtgataaa cttactttta gagcttctgt gccagaaaat tttgagcata ttcttattcc agaagaaaaa gtagcctctt tgacagaaaa agaaattgtt gcaaaaattg taggaaatgt tttcgtccaa aaaagtcagc aaaaaatcaa aactttatca aaatctgtct ataaaaactc gaaaatgttg gatgctgata tcaatcttgt taatatagat tcaaatctag gtgaacagta tattaggcag aatatcagtc cttttaaata tatgtctaaa ttgtcaggta cggaaatatt gggtattaac agtccacagc gtaaacaagc taagaaagat tactttaatc tcagtcagga gtttgataat tatataagtg ttgtactctc tactagagaa caaattcttc aagtgcacca agaaaaacaa gaacgttgtc agcaatcgat aaaaatgcct agtcaagcag tgcaaaatgt cctttctttg tctgaagaag atcagatcaa agctttaaat gctaatcctt cacttcacga agagattaca ggtcttttgg ataaggtcaa agatcgtctt acattttggg agtatcaagc ccttgatgat agagattatg agaaactctc taaaagtatc ggcatatcag tcagcagagc acagaaagtt acagaaatta ttaagaaagc tcaaaaggta tgtcagctaa cgagaagcag agctcatgag ttgaaagcga tggaatgtaa tcggttacaa atataa
Details for BaspA.17330.g.B1.GE35713
HARVESTED ON: 7/10/2012
SEQUENCED ON: 2/22/2016
EXPECTED MW: 59kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 59
Validated AA Sequence
MAHHHHHHML QGIEFRNVCA YDVLKAEYRL KKEPLPEKFD SSYLKYLHKC LFEETFEWAG HTRDIPFTFL DGSVTMVPVN DKIKDGLERI DQILAEKNNL KGLSRKEFVY EASMLFALFS NLRPFAVGNG CAQRIFMEKI AEAAGHRLDF SVVTEQRMRS ANNAALPHDG KAVGNITPMC HLFEDISNPE KVDVLREFMN AIRNRGSFDE YANIRNKIIV APNEGSTYTG IYKDSSPDSI MLMVNDKYVI CKKDYLAPEI LTALRLXDKL TFRASVPENF EHILIPEXKV ASLTEKXXXX KXXXXVFVXK S
Validated NT Sequence
atggctcacc accaccacca ccatatgttg caaggcatag agtttcgaaa tgtatgtgcc tatgatgtac taaaagcaga gtacaggcta aaaaaagaac cgctaccaga aaagtttgat tcgtcttatc taaaatatct tcataaatgt ttatttgaag agacgtttga atgggctggg catacccgtg acattccatt tacattttta gatggctcag ttaccatggt gccagtcaat gataaaatta aagacggttt agagagaatc gaccaaatac tagccgaaaa gaataatttg aaaggtttat cacgtaaaga gtttgtctat gaagcatcga tgctctttgc tttgtttagt aatttacgac ctttcgcggt tggaaatggg tgcgcacaac gaatatttat ggaaaaaata gccgaagcag cggggcatag gcttgacttc tcggtcgtga cagaacaacg catgcgatct gctaataatg ctgcgttacc acatgatggc aaagctgtag gcaatatcac tcctatgtgt catctgtttg aagatatctc caatccagaa aaagtggatg ttttaagaga attcatgaat gccataagga atcggggatc ttttgatgag tacgcaaata taagaaataa gattattgta gcgccaaatg aaggttctac ttatacaggc atctataaag atagtagccc agattctatt atgctcatgg tgaatgataa gtatgttata tgcaaaaaag attatcttgc accagaaata ttaacagcat tacggctnnn ngataaactt acttttagag cttctgtgcc agaaaatttt gagcatattc ttattccaga anaaaaagta gcctctttga cagaaaaana aantgnnnca aaanntgnnn nnnnngtttt cgtcnaaaaa agtcag
Expected Protein Sequence
MAHHHHHHML QGIEFRNVCA YDVLKAEYRL KKEPLPEKFD SSYLKYLHKC LFEETFEWAG HTRDIPFTFL DGSVTMVPVN DKIKDGLERI DQILAEKNNL KGLSRKEFVY EASMLFALFS NLRPFAVGNG CAQRIFMEKI AEAAGHRLDF SVVTEQRMRS ANNAALPHDG KAVGNITPMC HLFEDISNPE KVDVLREFMN AIRNRGSFDE YANIRNKIIV APNEGSTYTG IYKDSSPDSI MLMVNDKYVI CKKDYLAPEI LTALRLGDKL TFRASVPENF EHILIPEEKV ASLTEKEIVA KIVGNVFVQK SQQKIKTLSK SVYKNSKMLD ADINLVNIDS NLGEQYIRQN ISPFKYMSKL SGTEILGINS PQRKQAKKDY FNLSQEFDNY ISVVLSTREQ ILQVHQEKQE RCQQSIKMPS QAVQNVLSLS EEDQIKALNA NPSLHEEITG LLDKVKDRLT FWEYQALDDR DYEKLSKSIG ISVSRAQKVT EIIKKAQKVC QLTRSRAHEL KAMECNRLQI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc aaggcataga gtttcgaaat gtatgtgcct atgatgtact aaaagcagag tacaggctaa aaaaagaacc gctaccagaa aagtttgatt cgtcttatct aaaatatctt cataaatgtt tatttgaaga gacgtttgaa tgggctgggc atacccgtga cattccattt acatttttag atggctcagt taccatggtg ccagtcaatg ataaaattaa agacggttta gagagaatcg accaaatact agccgaaaag aataatttga aaggtttatc acgtaaagag tttgtctatg aagcatcgat gctctttgct ttgtttagta atttacgacc tttcgcggtt ggaaatgggt gcgcacaacg aatatttatg gaaaaaatag ccgaagcagc ggggcatagg cttgacttct cggtcgtgac agaacaacgc atgcgatctg ctaataatgc tgcgttacca catgatggca aagctgtagg caatatcact cctatgtgtc atctgtttga agatatctcc aatccagaaa aagtggatgt tttaagagaa ttcatgaatg ccataaggaa tcggggatct tttgatgagt acgcaaatat aagaaataag attattgtag cgccaaatga aggttctact tatacaggca tctataaaga tagtagccca gattctatta tgctcatggt gaatgataag tatgttatat gcaaaaaaga ttatcttgca ccagaaatat taacagcatt acggcttggt gataaactta cttttagagc ttctgtgcca gaaaattttg agcatattct tattccagaa gaaaaagtag cctctttgac agaaaaagaa attgttgcaa aaattgtagg aaatgttttc gtccaaaaaa gtcagcaaaa aatcaaaact ttatcaaaat ctgtctataa aaactcgaaa atgttggatg ctgatatcaa tcttgttaat atagattcaa atctaggtga acagtatatt aggcagaata tcagtccttt taaatatatg tctaaattgt caggtacgga aatattgggt attaacagtc cacagcgtaa acaagctaag aaagattact ttaatctcag tcaggagttt gataattata taagtgttgt actctctact agagaacaaa ttcttcaagt gcaccaagaa aaacaagaac gttgtcagca atcgataaaa atgcctagtc aagcagtgca aaatgtcctt tctttgtctg aagaagatca gatcaaagct ttaaatgcta atccttcact tcacgaagag attacaggtc ttttggataa ggtcaaagat cgtcttacat tttgggagta tcaagccctt gatgatagag attatgagaa actctctaaa agtatcggca tatcagtcag cagagcacag aaagttacag aaattattaa gaaagctcaa aaggtatgtc agctaacgag aagcagagct catgagttga aagcgatgga atgtaatcgg ttacaaatat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaspA.17330.g.B3.GE37058
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML QGIEFRNVCA YDVLKAEYRL KKEPLPEKFD SSYLKYLHKC LFEETFEWAG HTRDIPFTFL DGSVTMVPVN DKIKDGLERI DQILAEKNNL KGLSRKEFVY EASMLFALFS NLRPFAVGNG CAQRIFMEKI AEAAGHRLDF SVVTEQRMRS ANNAALPHDG KAVGNITPMC HLFEDISNPE KVDVLREFMN AIRNRGSFDE YANIRNKIIV APNEGSTYTG IYKDSSPDSI MLMVNDKYVI CKKXYLAPEI LTALRLGDKL TFR
Validated NT Sequence
ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatgttg caaggcatag agtttcgaaa tgtatgtgcc tatgatgtac taaaagcaga gtacaggcta aaaaaagaac cgctaccaga aaagtttgat tcgtcttatc taaaatatct tcataaatgt ttatttgaag agacgtttga atgggctggg catacccgtg acattccatt tacattttta gatggctcag ttaccatggt gccagtcaat gataaaatta aagacggttt agagagaatc gaccaaatac tagccgaaaa gaataatttg aaaggtttat cacgtaaaga gtttgtctat gaagcatcga tgctctttgc tttgtttagt aatttacgac ctttcgcggt tggaaatggg tgcgcacaac gaatatttat ggaaaaaata gccgaagcag cggggcatag gcttgacttc tcggtcgtga cagaacaacg catgcgatct gctaataatg ctgcgttacc acatgatggc aaagctgtag gcaatatcac tcctatgtgt catctgtttg aagatatctc caatccagaa aaagtggatg ttttaagaga attcatgaat gccataagga atcggggatc ttttgatgag tacgcaaata taagaaataa gattattgta gcgccaaatg aaggttctac ttatacaggc atctataaag atagtagccc agattctatt atgctcatgg tgaatgataa gtatgttata tgcaaaaaag antatcttgc accagaaata ttaacagcat tacggcttgg tgataaactt acttttagat aagtgagtaa gataggatcc ggctgctacn nnnccngaan g
Expected Protein Sequence
MAHHHHHHMQ GIEFRNVCAY DVLKAEYRLK KEPLPEKFDS SYLKYLHKCL FEETFEWAGH TRDIPFTFLD GSVTMVPVND KIKDGLERID QILAEKNNLK GLSRKEFVYE ASMLFALFSN LRPFAVGNGC AQRIFMEKIA EAAGHRLDFS VVTEQRMRSA NNAALPHDGK AVGNITPMCH LFEDISNPEK VDVLREFMNA IRNRGSFDEY ANIRNKIIVA PNEGSTYTGI YKDSSPDSIM LMVNDKYVIC KKDYLAPEIL TALRLGDKLT FR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcaag gcatagagtt tcgaaatgta tgtgcctatg atgtactaaa agcagagtac aggctaaaaa aagaaccgct accagaaaag tttgattcgt cttatctaaa atatcttcat aaatgtttat ttgaagagac gtttgaatgg gctgggcata cccgtgacat tccatttaca tttttagatg gctcagttac catggtgcca gtcaatgata aaattaaaga cggtttagag agaatcgacc aaatactagc cgaaaagaat aatttgaaag gtttatcacg taaagagttt gtctatgaag catcgatgct ctttgctttg tttagtaatt tacgaccttt cgcggttgga aatgggtgcg cacaacgaat atttatggaa aaaatagccg aagcagcggg gcataggctt gacttctcgg tcgtgacaga acaacgcatg cgatctgcta ataatgctgc gttaccacat gatggcaaag ctgtaggcaa tatcactcct atgtgtcatc tgtttgaaga tatctccaat ccagaaaaag tggatgtttt aagagaattc atgaatgcca taaggaatcg gggatctttt gatgagtacg caaatataag aaataagatt attgtagcgc caaatgaagg ttctacttat acaggcatct ataaagatag tagcccagat tctattatgc tcatggtgaa tgataagtat gttatatgca aaaaagatta tcttgcacca gaaatattaa cagcattacg gcttggtgat aaacttactt ttagatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BaspA.17330.g.B1.PW36353
PURIFICATION DATe: 12/13/2012
CONCENTRATION: 15.2mg/ml
OBSERVED MW: 56kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 59
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML QGIEFRNVCA YDVLKAEYRL KKEPLPEKFD SSYLKYLHKC LFEETFEWAG HTRDIPFTFL DGSVTMVPVN DKIKDGLERI DQILAEKNNL KGLSRKEFVY EASMLFALFS NLRPFAVGNG CAQRIFMEKI AEAAGHRLDF SVVTEQRMRS ANNAALPHDG KAVGNITPMC HLFEDISNPE KVDVLREFMN AIRNRGSFDE YANIRNKIIV APNEGSTYTG IYKDSSPDSI MLMVNDKYVI CKKDYLAPEI LTALRLXDKL TFRASVPENF EHILIPEXKV ASLTEKXXXX KXXXXVFVXK S
Validated NT Sequence
atggctcacc accaccacca ccatatgttg caaggcatag agtttcgaaa tgtatgtgcc tatgatgtac taaaagcaga gtacaggcta aaaaaagaac cgctaccaga aaagtttgat tcgtcttatc taaaatatct tcataaatgt ttatttgaag agacgtttga atgggctggg catacccgtg acattccatt tacattttta gatggctcag ttaccatggt gccagtcaat gataaaatta aagacggttt agagagaatc gaccaaatac tagccgaaaa gaataatttg aaaggtttat cacgtaaaga gtttgtctat gaagcatcga tgctctttgc tttgtttagt aatttacgac ctttcgcggt tggaaatggg tgcgcacaac gaatatttat ggaaaaaata gccgaagcag cggggcatag gcttgacttc tcggtcgtga cagaacaacg catgcgatct gctaataatg ctgcgttacc acatgatggc aaagctgtag gcaatatcac tcctatgtgt catctgtttg aagatatctc caatccagaa aaagtggatg ttttaagaga attcatgaat gccataagga atcggggatc ttttgatgag tacgcaaata taagaaataa gattattgta gcgccaaatg aaggttctac ttatacaggc atctataaag atagtagccc agattctatt atgctcatgg tgaatgataa gtatgttata tgcaaaaaag attatcttgc accagaaata ttaacagcat tacggctnnn ngataaactt acttttagag cttctgtgcc agaaaatttt gagcatattc ttattccaga anaaaaagta gcctctttga cagaaaaana aantgnnnca aaanntgnnn nnnnngtttt cgtcnaaaaa agtcag
Expressed Protein Sequence
MAHHHHHHML QGIEFRNVCA YDVLKAEYRL KKEPLPEKFD SSYLKYLHKC LFEETFEWAG HTRDIPFTFL DGSVTMVPVN DKIKDGLERI DQILAEKNNL KGLSRKEFVY EASMLFALFS NLRPFAVGNG CAQRIFMEKI AEAAGHRLDF SVVTEQRMRS ANNAALPHDG KAVGNITPMC HLFEDISNPE KVDVLREFMN AIRNRGSFDE YANIRNKIIV APNEGSTYTG IYKDSSPDSI MLMVNDKYVI CKKDYLAPEI LTALRLGDKL TFRASVPENF EHILIPEEKV ASLTEKEIVA KIVGNVFVQK SQQKIKTLSK SVYKNSKMLD ADINLVNIDS NLGEQYIRQN ISPFKYMSKL SGTEILGINS PQRKQAKKDY FNLSQEFDNY ISVVLSTREQ ILQVHQEKQE RCQQSIKMPS QAVQNVLSLS EEDQIKALNA NPSLHEEITG LLDKVKDRLT FWEYQALDDR DYEKLSKSIG ISVSRAQKVT EIIKKAQKVC QLTRSRAHEL KAMECNRLQI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGC AAGGCATAGA GTTTCGAAAT GTATGTGCCT ATGATGTACT AAAAGCAGAG TACAGGCTAA AAAAAGAACC GCTACCAGAA AAGTTTGATT CGTCTTATCT AAAATATCTT CATAAATGTT TATTTGAAGA GACGTTTGAA TGGGCTGGGC ATACCCGTGA CATTCCATTT ACATTTTTAG ATGGCTCAGT TACCATGGTG CCAGTCAATG ATAAAATTAA AGACGGTTTA GAGAGAATCG ACCAAATACT AGCCGAAAAG AATAATTTGA AAGGTTTATC ACGTAAAGAG TTTGTCTATG AAGCATCGAT GCTCTTTGCT TTGTTTAGTA ATTTACGACC TTTCGCGGTT GGAAATGGGT GCGCACAACG AATATTTATG GAAAAAATAG CCGAAGCAGC GGGGCATAGG CTTGACTTCT CGGTCGTGAC AGAACAACGC ATGCGATCTG CTAATAATGC TGCGTTACCA CATGATGGCA AAGCTGTAGG CAATATCACT CCTATGTGTC ATCTGTTTGA AGATATCTCC AATCCAGAAA AAGTGGATGT TTTAAGAGAA TTCATGAATG CCATAAGGAA TCGGGGATCT TTTGATGAGT ACGCAAATAT AAGAAATAAG ATTATTGTAG CGCCAAATGA AGGTTCTACT TATACAGGCA TCTATAAAGA TAGTAGCCCA GATTCTATTA TGCTCATGGT GAATGATAAG TATGTTATAT GCAAAAAAGA TTATCTTGCA CCAGAAATAT TAACAGCATT ACGGCTTGGT GATAAACTTA CTTTTAGAGC TTCTGTGCCA GAAAATTTTG AGCATATTCT TATTCCAGAA GAAAAAGTAG CCTCTTTGAC AGAAAAAGAA ATTGTTGCAA AAATTGTAGG AAATGTTTTC GTCCAAAAAA GTCAGCAAAA AATCAAAACT TTATCAAAAT CTGTCTATAA AAACTCGAAA ATGTTGGATG CTGATATCAA TCTTGTTAAT ATAGATTCAA ATCTAGGTGA ACAGTATATT AGGCAGAATA TCAGTCCTTT TAAATATATG TCTAAATTGT CAGGTACGGA AATATTGGGT ATTAACAGTC CACAGCGTAA ACAAGCTAAG AAAGATTACT TTAATCTCAG TCAGGAGTTT GATAATTATA TAAGTGTTGT ACTCTCTACT AGAGAACAAA TTCTTCAAGT GCACCAAGAA AAACAAGAAC GTTGTCAGCA ATCGATAAAA ATGCCTAGTC AAGCAGTGCA AAATGTCCTT TCTTTGTCTG AAGAAGATCA GATCAAAGCT TTAAATGCTA ATCCTTCACT TCACGAAGAG ATTACAGGTC TTTTGGATAA GGTCAAAGAT CGTCTTACAT TTTGGGAGTA TCAAGCCCTT GATGATAGAG ATTATGAGAA ACTCTCTAAA AGTATCGGCA TATCAGTCAG CAGAGCACAG AAAGTTACAG AAATTATTAA GAAAGCTCAA AAGGTATGTC AGCTAACGAG AAGCAGAGCT CATGAGTTGA AAGCGATGGA ATGTAATCGG TTACAAATAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT