BaspA.17330.e
Bep8 protein (VirB-translocated Bartonella effector protein)
| CENTER ID: | BaspA.17330.e |
| ORGANISM: | Bartonella sp. 1-1C |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaspA.17330.e.B2.GE37051 | FIC | 10 | 241 | ||
| BaspA.17330.e.B3.GE37052 | FIC and OB-Fold | 10 | 297 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaspA.17330.e.B2.PS01912 | FIC | 10 | 241 | ||
| BaspA.17330.e.B2.PS01913 | FIC | 10 | 241 |
Structures
4PY3
DEPOSITED: 3/25/2014
DETERMINATION: XRay
CLONE: BaspA.17330.e.B2.GE37051
PROTEIN: BaspA.17330.e.B2.PS01912
Publications by SSGCID
Evolutionary Diversification of Host-Targeted Bartonella Effectors Proteins Derived from a Conserved FicTA Toxin-Antitoxin Module
de Beer T, Dehio C, Edwards T, Harms A, Myler P, Phan I, Schirmer T, Dranow D, Tamegger S, Dietz N
Microorganisms - 2021
volume 9, issue 8, pages 1645
PMID: 34442725; PMCID: PMC8401265
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_171090 |
| UniProt: | E6YV77 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVTFSDISLA RNYAYPHMNT LKNKHNIMST KKLAHVCEHY AKKAIINLNK EPLPQKFDSS YLKYIHQRLF ESTFEWAGYT RDFSFTFDDG TVAEMPMMKV PNLDIFYVQG NDIQENLKKF DQLLASKNNL QGLSREEFVD EAAKLFVFLN SIAPFRAGNE PTQRVFFEKL AEAAGHQLDF SVATEKRIMR ACIDGMTLKD NMAYKEMKSL FEDISDPKKI AALKNFLRRI PRLERERLND EYVIMPKEGM TYTGIYNVSS VNFVIIKVAD SCVLCTKDYF APEQLKVLKP GAECTVTVPT EKDLNQILIP AEKLTSLKEE EIVKKIKLSD YVKESQKKVD RFLKLVYGDV AVLDEYMLSI HERPEINKQL CEQIRKSPQN FAKLSGFKIF GIKSSKRRIA EENVSRLVQE IEKHAEIVKS VKDEIVLEFE KEQKRVEQVV KVSKEIQDIL NLPKEEQKKA LESSPVLYEE LCSFLKQVDS RLSAKEKDCI YGYNNRKLSE SIGVSENKAA IIIKTVNKSI KLREQFEKIA KEIKVDRSEI MAIAV
NT Sequence
atggtaacat tttctgatat atctttagct cgtaattatg cttatcctca catgaacaca ctaaagaata aacataatat aatgagtact aaaaagcttg cacacgtgtg cgagcattat gcaaaaaaag caataatcaa tttaaataag gagccattac cacaaaaatt tgattcctct tatttaaaat atatccatca gcgcttattc gagagtacat ttgagtgggc tggatatact cgtgattttt cgtttacgtt tgatgatggc actgttgctg aaatgccaat gatgaaagtt ccaaacttag atatttttta tgtacaaggt aacgacattc aagaaaactt aaagaaattt gatcaattac ttgcttcaaa gaataatttg caaggtttat cgcgtgaaga gtttgttgat gaagcagcaa aactttttgt tttcctcaat tctatagctc ctttcagggc tggtaatgag cctacacaac gagtattttt tgaaaaactt gctgaggctg caggtcatca actagatttt tcagttgcga cagaaaaacg tataatgcgt gcctgtattg atggaatgac tctaaaggat aatatggctt ataaagagat gaaatctctg tttgaagata tttctgatcc aaaaaaaata gccgctttaa agaattttct tagacgcata cctagattag agcgtgaacg tttgaacgat gaatatgtta taatgccaaa ggaaggtatg acctatacag gtatttataa tgtaagtagt gtaaattttg ttattatcaa agtagccgat tcttgtgttc tgtgcacgaa agattatttt gcaccagaac aacttaaggt attaaaacct ggagctgagt gtaccgttac agttccaaca gaaaaagact taaatcaaat tctcattcca gctgaaaaat taacttcttt aaaggaagaa gaaatagtta aaaagattaa gctttctgat tatgttaaag aaagccaaaa gaaagttgat cgttttttaa agcttgttta tggagacgta gcagtattag acgaatatat gctatctatt catgagcgtc ctgaaataaa taaacagttg tgcgaacaga ttagaaaatc tcctcaaaat tttgctaagc tctctggctt taaaatattc ggtataaaaa gttcaaaacg tagaatagct gaagaaaatg tgtcaagact cgttcaagaa attgaaaaac atgcggaaat tgtgaaatct gttaaagatg aaattgttct agaatttgaa aaagaacaaa aacgtgttga gcaagtggta aaagttagca aagagataca agatattctt aatttgccaa aggaagagca gaaaaaagct ttagagtcct ctcctgtcct ttacgaagag ctttgcagct ttctaaagca agtcgattct cgtctttcag caaaagagaa agactgtatc tatggctata ataatagaaa actttccgaa agtattggtg tatcggaaaa taaagcagcg ataattataa agactgttaa taaaagtata aaattacgcg aacaatttga aaagatcgca aaagaaataa aagttgatcg ttcagaaata atggctatag ccgtttga
Details for BaspA.17330.e.B2.GE37051
| HARVESTED ON: | 6/12/2013 |
| SEQUENCED ON: | 6/11/2013 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA RNYAYPHMNT LKNKHNIMST KKLAHVCEHY AKKAIINLNK EPLPQKFDSS YLKYIHQRLF ESTFEWAGYT RDFSFTFDDG TVAEMPMMKV PNLDIFYVQG NDIQENLKKF DQLLASKNNL QGLSREEFVD EAAKLFVFLN SIAPFRAGNE PTQRVFFEKL AEAAGHQLDF SVATEKRIMR ACIDGMTLKD NMAYKEMKSL FEDISDPKKI AALKNFLRRI PRLERERLND E
Validated NT Sequence
tcttactcac ttattcatcg ttcaaacgtt cacgctctaa tctaggtatg cgtctaagaa aattctttaa agcggctatt ttttttggat cagaaatatc ttcaaacaga gatttcatct ctttataagc catattatcc tttagagtca ttccatcaat acaggcacgc attatacgtt tttctgtcgc aactgaaaaa tctagttgat gacctgcagc ctcagcaagt ttttcaaaaa atactcgttg tgtaggctca ttaccagccc tgaaaggagc tatagaattg aggaaaacaa aaagttttgc tgcttcatca acaaactctt cacgcgataa accttgcaaa ttattctttg aagcaagtaa ttgatcaaat ttctttaagt tttcttgaat gtcgttacct tgtacataaa aaatatctaa gtttggaact ttcatcattg gcatttcagc aacagtgcca tcatcaaacg taaacgaaaa atcacgagta tatccagccc actcaaatgt actctcgaat aagcgctgat ggatatattt taaataagag gaatcaaatt tttgtggtaa tggctcctta tttaaattga ttattgcttt ttttgcataa tgctcgcaca cgtgtgcaag ctttttagta ctcattatat tatgtttatt ctttagtgtg ttcatgtgag gataagcata attacgagcc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHAR NYAYPHMNTL KNKHNIMSTK KLAHVCEHYA KKAIINLNKE PLPQKFDSSY LKYIHQRLFE STFEWAGYTR DFSFTFDDGT VAEMPMMKVP NLDIFYVQGN DIQENLKKFD QLLASKNNLQ GLSREEFVDE AAKLFVFLNS IAPFRAGNEP TQRVFFEKLA EAAGHQLDFS VATEKRIMRA CIDGMTLKDN MAYKEMKSLF EDISDPKKIA ALKNFLRRIP RLERERLNDE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctcgta attatgctta tcctcacatg aacacactaa agaataaaca taatataatg agtactaaaa agcttgcaca cgtgtgcgag cattatgcaa aaaaagcaat aatcaattta aataaggagc cattaccaca aaaatttgat tcctcttatt taaaatatat ccatcagcgc ttattcgaga gtacatttga gtgggctgga tatactcgtg atttttcgtt tacgtttgat gatggcactg ttgctgaaat gccaatgatg aaagttccaa acttagatat tttttatgta caaggtaacg acattcaaga aaacttaaag aaatttgatc aattacttgc ttcaaagaat aatttgcaag gtttatcgcg tgaagagttt gttgatgaag cagcaaaact ttttgttttc ctcaattcta tagctccttt cagggctggt aatgagccta cacaacgagt attttttgaa aaacttgctg aggctgcagg tcatcaacta gatttttcag ttgcgacaga aaaacgtata atgcgtgcct gtattgatgg aatgactcta aaggataata tggcttataa agagatgaaa tctctgtttg aagatatttc tgatccaaaa aaaatagccg ctttaaagaa ttttcttaga cgcataccta gattagagcg tgaacgtttg aacgatgaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaspA.17330.e.B3.GE37052
| HARVESTED ON: | 6/12/2013 |
| SEQUENCED ON: | 6/11/2013 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA RNYAYPHMNT LKNKHNIMST KKLAHVCEHY AKKAIINLNK EPLPQKFDSS YLKYIHQRLF ESTFEWAGYT RDFSFTFDDG TVAEMPMMKV PNLDIFYVQG NDIQENLKKF DQLLASKNNL QGLSREEFVD EAAKLFVFLN SIAPFRAGNE PTQRVFFEKL AEAAGHQLDF SVATEKRIMR ACIDGMTLKD NMAYKEMKSL FEDISDPKKI AALKNFLRRI PRXERERLND EYVIMPKEGM TYTGIYNVSS VNFVIIKVAX SCVLCTKXYF APEQLXXLKP GXXCTVT
Validated NT Sequence
tttgtttact ttaagaagga gatataccat ggctcaccac caccaccacc atatggctcg taattatgct tatcctcaca tgaacacact aaagaataaa cataatataa tgagtactaa aaagcttgca cacgtgtgcg agcattatgc aaaaaaagca ataatcaatt taaataagga gccattacca caaaaatttg attcctctta tttaaaatat atccatcagc gcttattcga gagtacattt gagtgggctg gatatactcg tgatttttcg tttacgtttg atgatggcac tgttgctgaa atgccaatga tgaaagttcc aaacttagat attttttatg tacaaggtaa cgacattcaa gaaaacttaa agaaatttga tcaattactt gcttcaaaga ataatttgca aggtttatcg cgtgaagagt ttgttgatga agcagcaaaa ctttttgttt tcctcaattc tatagctcct ttcagggctg gtaatgagcc tacacaacga gtattttttg aaaaacttgc tgaggctgca ggtcatcaac tagatttttc agttgcgaca gaaaaacgta taatgcgtgc ctgtattgat ggaatgactc taaaggataa tatggcttat aaagagatga aatctctgtt tgaagatatt tctgatccaa aaaaaatagc cgctttaaag aattttctta gacgcatacc tagatnagag cgtgaacgtt tgaacgatga atatgttata atgccaaagg aaggtatgac ctatacaggt atttataatg taagtagtgt aaattttgtt attatcaaag tagccnantc ttgtgttctg tgcacgaaag antattttgc accagaacaa cttnnnnnat taaaacctgg nnctgantgt accgttacat aantgagtaa nataggatcc ggctgctaac aaagcccgaa aggaagctga nttggctgct gcncgctgan caataactag catnacccct tggggctnna cnnnnntnnn ngnggnnnan nnnnnnnnnn nnnannnnnn nnnnnnnnnn nnnnnnnnnn ncntnnngnn nnnntnnnnn nnnnnnnnnn nnnnnggnnn actnnnnnng ntnnngnnnn nnnannnnnn nnnnnannnn nnnnngnnnn nannnannaa annnnnnnc
Expected Protein Sequence
MAHHHHHHAR NYAYPHMNTL KNKHNIMSTK KLAHVCEHYA KKAIINLNKE PLPQKFDSSY LKYIHQRLFE STFEWAGYTR DFSFTFDDGT VAEMPMMKVP NLDIFYVQGN DIQENLKKFD QLLASKNNLQ GLSREEFVDE AAKLFVFLNS IAPFRAGNEP TQRVFFEKLA EAAGHQLDFS VATEKRIMRA CIDGMTLKDN MAYKEMKSLF EDISDPKKIA ALKNFLRRIP RLERERLNDE YVIMPKEGMT YTGIYNVSSV NFVIIKVADS CVLCTKDYFA PEQLKVLKPG AECTVT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctcgta attatgctta tcctcacatg aacacactaa agaataaaca taatataatg agtactaaaa agcttgcaca cgtgtgcgag cattatgcaa aaaaagcaat aatcaattta aataaggagc cattaccaca aaaatttgat tcctcttatt taaaatatat ccatcagcgc ttattcgaga gtacatttga gtgggctgga tatactcgtg atttttcgtt tacgtttgat gatggcactg ttgctgaaat gccaatgatg aaagttccaa acttagatat tttttatgta caaggtaacg acattcaaga aaacttaaag aaatttgatc aattacttgc ttcaaagaat aatttgcaag gtttatcgcg tgaagagttt gttgatgaag cagcaaaact ttttgttttc ctcaattcta tagctccttt cagggctggt aatgagccta cacaacgagt attttttgaa aaacttgctg aggctgcagg tcatcaacta gatttttcag ttgcgacaga aaaacgtata atgcgtgcct gtattgatgg aatgactcta aaggataata tggcttataa agagatgaaa tctctgtttg aagatatttc tgatccaaaa aaaatagccg ctttaaagaa ttttcttaga cgcataccta gattagagcg tgaacgtttg aacgatgaat atgttataat gccaaaggaa ggtatgacct atacaggtat ttataatgta agtagtgtaa attttgttat tatcaaagta gccgattctt gtgttctgtg cacgaaagat tattttgcac cagaacaact taaggtatta aaacctggag ctgagtgtac cgttacatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BaspA.17330.e.B2.PS01912
| PURIFICATION DATe: | 10/10/2013 |
| CONCENTRATION: | 22mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA RNYAYPHMNT LKNKHNIMST KKLAHVCEHY AKKAIINLNK EPLPQKFDSS YLKYIHQRLF ESTFEWAGYT RDFSFTFDDG TVAEMPMMKV PNLDIFYVQG NDIQENLKKF DQLLASKNNL QGLSREEFVD EAAKLFVFLN SIAPFRAGNE PTQRVFFEKL AEAAGHQLDF SVATEKRIMR ACIDGMTLKD NMAYKEMKSL FEDISDPKKI AALKNFLRRI PRLERERLND E
Validated NT Sequence
tcttactcac ttattcatcg ttcaaacgtt cacgctctaa tctaggtatg cgtctaagaa aattctttaa agcggctatt ttttttggat cagaaatatc ttcaaacaga gatttcatct ctttataagc catattatcc tttagagtca ttccatcaat acaggcacgc attatacgtt tttctgtcgc aactgaaaaa tctagttgat gacctgcagc ctcagcaagt ttttcaaaaa atactcgttg tgtaggctca ttaccagccc tgaaaggagc tatagaattg aggaaaacaa aaagttttgc tgcttcatca acaaactctt cacgcgataa accttgcaaa ttattctttg aagcaagtaa ttgatcaaat ttctttaagt tttcttgaat gtcgttacct tgtacataaa aaatatctaa gtttggaact ttcatcattg gcatttcagc aacagtgcca tcatcaaacg taaacgaaaa atcacgagta tatccagccc actcaaatgt actctcgaat aagcgctgat ggatatattt taaataagag gaatcaaatt tttgtggtaa tggctcctta tttaaattga ttattgcttt ttttgcataa tgctcgcaca cgtgtgcaag ctttttagta ctcattatat tatgtttatt ctttagtgtg ttcatgtgag gataagcata attacgagcc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHAR NYAYPHMNTL KNKHNIMSTK KLAHVCEHYA KKAIINLNKE PLPQKFDSSY LKYIHQRLFE STFEWAGYTR DFSFTFDDGT VAEMPMMKVP NLDIFYVQGN DIQENLKKFD QLLASKNNLQ GLSREEFVDE AAKLFVFLNS IAPFRAGNEP TQRVFFEKLA EAAGHQLDFS VATEKRIMRA CIDGMTLKDN MAYKEMKSLF EDISDPKKIA ALKNFLRRIP RLERERLNDE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCTCGTA ATTATGCTTA TCCTCACATG AACACACTAA AGAATAAACA TAATATAATG AGTACTAAAA AGCTTGCACA CGTGTGCGAG CATTATGCAA AAAAAGCAAT AATCAATTTA AATAAGGAGC CATTACCACA AAAATTTGAT TCCTCTTATT TAAAATATAT CCATCAGCGC TTATTCGAGA GTACATTTGA GTGGGCTGGA TATACTCGTG ATTTTTCGTT TACGTTTGAT GATGGCACTG TTGCTGAAAT GCCAATGATG AAAGTTCCAA ACTTAGATAT TTTTTATGTA CAAGGTAACG ACATTCAAGA AAACTTAAAG AAATTTGATC AATTACTTGC TTCAAAGAAT AATTTGCAAG GTTTATCGCG TGAAGAGTTT GTTGATGAAG CAGCAAAACT TTTTGTTTTC CTCAATTCTA TAGCTCCTTT CAGGGCTGGT AATGAGCCTA CACAACGAGT ATTTTTTGAA AAACTTGCTG AGGCTGCAGG TCATCAACTA GATTTTTCAG TTGCGACAGA AAAACGTATA ATGCGTGCCT GTATTGATGG AATGACTCTA AAGGATAATA TGGCTTATAA AGAGATGAAA TCTCTGTTTG AAGATATTTC TGATCCAAAA AAAATAGCCG CTTTAAAGAA TTTTCTTAGA CGCATACCTA GATTAGAGCG TGAACGTTTG AACGATGAAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT
Details for BaspA.17330.e.B2.PS01913
| PURIFICATION DATe: | 10/10/2013 |
| CONCENTRATION: | 20.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA RNYAYPHMNT LKNKHNIMST KKLAHVCEHY AKKAIINLNK EPLPQKFDSS YLKYIHQRLF ESTFEWAGYT RDFSFTFDDG TVAEMPMMKV PNLDIFYVQG NDIQENLKKF DQLLASKNNL QGLSREEFVD EAAKLFVFLN SIAPFRAGNE PTQRVFFEKL AEAAGHQLDF SVATEKRIMR ACIDGMTLKD NMAYKEMKSL FEDISDPKKI AALKNFLRRI PRLERERLND E
Validated NT Sequence
tcttactcac ttattcatcg ttcaaacgtt cacgctctaa tctaggtatg cgtctaagaa aattctttaa agcggctatt ttttttggat cagaaatatc ttcaaacaga gatttcatct ctttataagc catattatcc tttagagtca ttccatcaat acaggcacgc attatacgtt tttctgtcgc aactgaaaaa tctagttgat gacctgcagc ctcagcaagt ttttcaaaaa atactcgttg tgtaggctca ttaccagccc tgaaaggagc tatagaattg aggaaaacaa aaagttttgc tgcttcatca acaaactctt cacgcgataa accttgcaaa ttattctttg aagcaagtaa ttgatcaaat ttctttaagt tttcttgaat gtcgttacct tgtacataaa aaatatctaa gtttggaact ttcatcattg gcatttcagc aacagtgcca tcatcaaacg taaacgaaaa atcacgagta tatccagccc actcaaatgt actctcgaat aagcgctgat ggatatattt taaataagag gaatcaaatt tttgtggtaa tggctcctta tttaaattga ttattgcttt ttttgcataa tgctcgcaca cgtgtgcaag ctttttagta ctcattatat tatgtttatt ctttagtgtg ttcatgtgag gataagcata attacgagcc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHAR NYAYPHMNTL KNKHNIMSTK KLAHVCEHYA KKAIINLNKE PLPQKFDSSY LKYIHQRLFE STFEWAGYTR DFSFTFDDGT VAEMPMMKVP NLDIFYVQGN DIQENLKKFD QLLASKNNLQ GLSREEFVDE AAKLFVFLNS IAPFRAGNEP TQRVFFEKLA EAAGHQLDFS VATEKRIMRA CIDGMTLKDN MAYKEMKSLF EDISDPKKIA ALKNFLRRIP RLERERLNDE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCTCGTA ATTATGCTTA TCCTCACATG AACACACTAA AGAATAAACA TAATATAATG AGTACTAAAA AGCTTGCACA CGTGTGCGAG CATTATGCAA AAAAAGCAAT AATCAATTTA AATAAGGAGC CATTACCACA AAAATTTGAT TCCTCTTATT TAAAATATAT CCATCAGCGC TTATTCGAGA GTACATTTGA GTGGGCTGGA TATACTCGTG ATTTTTCGTT TACGTTTGAT GATGGCACTG TTGCTGAAAT GCCAATGATG AAAGTTCCAA ACTTAGATAT TTTTTATGTA CAAGGTAACG ACATTCAAGA AAACTTAAAG AAATTTGATC AATTACTTGC TTCAAAGAAT AATTTGCAAG GTTTATCGCG TGAAGAGTTT GTTGATGAAG CAGCAAAACT TTTTGTTTTC CTCAATTCTA TAGCTCCTTT CAGGGCTGGT AATGAGCCTA CACAACGAGT ATTTTTTGAA AAACTTGCTG AGGCTGCAGG TCATCAACTA GATTTTTCAG TTGCGACAGA AAAACGTATA ATGCGTGCCT GTATTGATGG AATGACTCTA AAGGATAATA TGGCTTATAA AGAGATGAAA TCTCTGTTTG AAGATATTTC TGATCCAAAA AAAATAGCCG CTTTAAAGAA TTTTCTTAGA CGCATACCTA GATTAGAGCG TGAACGTTTG AACGATGAAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT