BaspA.17330.d

Bep3 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaspA.17330.d
ORGANISM: Bartonella sp. 1-1C
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BaspA.17330.d.B2.GE37048 FIC 87 318
BaspA.17330.d.B3.GE37049 FIC and extended OB-Fold 87 380
BaspA.17330.d.B4.GE37050 BID 395 535
BaspA.17330.d.B5.GE37451 N-term extended FIC 1 318
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: E6YV82

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRKSKDSPSL TVEELRRRRQ IIDSAIHTHT IERIPLHPDT LLVLEKYAEG GSLNEFNILM DSVIQMILQE MGNVQVEKLQ SASLSSYDNY IYPASHVLKN KYGIIDSDNL SMVSGHHAAK AIVNLHHEPL PERFDSFYLR YIHKRLFENT FEWAGNSRNF PFTFNDGTTA IIQTMKKVNS DDQFLESKKI AQYLDNVDQT LAEQNNFQGL SRQAFISKAA SMFALINYIH PFRDGNGRTQ RVFFERLAEA AGHQLDFSIV TAERMRVCSI LSMVRSNVMG DIGAMKHMFE DISNPEKVSI MKEFISSMSK SEYKNAQKMI IVMPKRGHNY LSFYESETAE HLLLKVDLNY ISKPLYVIFK KDYFLPNEVK ELKSGCPLSF KVPMSRDIKD LESILIPKET VASLTSDQLI EKITSHLAVQ LKRQEVDICA KYVYKNLEDF NEKISVKNIL ENRNFQEVFT KRIVDFPESI SKLAGTKILW LKTLKYKIAE QNVEALAQKV HDYVDIVKEV ENGIIKENLI KKKCLMTVVA MPSKKLQDIF NLSKDMQKET LSSSPSLQEE LNIFIKAVNQ RLMPLEHKWL RDANYDLLAE SIGISQSKAK QIGELFMQGK RLQNLLKEIK RDHSEVINMA C
NT Sequence
atgagaaaat ccaaagattc tccatctcta acagtagaag agcttcgaag acgtcgccaa attattgatt cagcgattca tactcatact attgaacgta tccctttgca tcctgacaca cttctagttt tagaaaaata tgcagaggga ggttcattaa atgagtttaa tatccttatg gatagtgtta tacaaatgat attgcaagaa atgggaaatg tacaggtgga aaaactacaa tctgcctctt tatcatcgta cgacaactat atttatcctg caagccacgt actaaaaaat aaatatggaa taatagactc agacaattta tccatggtaa gcggacatca tgcagcgaaa gcaatagtta atttgcacca tgaaccacta ccagaaaggt tcgattcttt ctatctaaga tatatccata aacgcttgtt tgagaataca tttgaatggg ctgggaatag tcgtaatttc ccatttacat ttaatgatgg cactactgct attatacaaa caatgaaaaa ggtaaattct gacgatcagt ttttagagag taaaaaaatt gctcaatacc tagataatgt ggatcaaacg cttgcagaac agaataattt tcaaggctta tcgcgacaag cttttattag taaagcagcg agtatgtttg ctcttattaa ttatatacac ccattcagag atggtaatgg tcgcacacaa cgagtgtttt ttgaaagact tgctgaagct gcaggtcatc aactagattt ctcaattgtt acagcagagc gtatgagagt ttgcagtata ctttctatgg tgcgttctaa tgttatgggc gatataggtg ctatgaagca tatgtttgaa gatatttcaa atccagaaaa agtaagtatt atgaaagagt tcataagctc catgagtaag tctgagtata aaaatgctca aaaaatgatt attgtaatgc caaaaagagg gcataactat ctaagttttt atgaaagtga gactgcagaa catcttctac tcaaagtaga tcttaattat atttcaaaac cgctttatgt gatattcaaa aaagattatt ttttacccaa tgaagttaag gaattaaagt ctggttgtcc gctaagtttt aaagttccta tgagtagaga tataaaagat ttggaaagta ttcttattcc aaaagaaacg gtagcttctt taacatcaga tcaacttatt gaaaaaatta cgagtcatct cgctgtccaa ttaaagcgac aagaagttga tatttgtgca aagtatgttt ataaaaattt agaagacttt aatgaaaaga taagtgtaaa aaacattctt gaaaatagaa attttcagga agtttttaca aagagaatag ttgattttcc tgagtctatt tctaaattag caggcacaaa aatattatgg ttgaaaactt taaaatacaa aattgctgaa caaaatgttg aagcacttgc tcaaaaggtt catgattatg tcgatattgt aaaagaggta gaaaatggaa ttattaaaga gaatctaata aagaaaaaat gtcttatgac agtagtagca atgcctagca aaaaattgca agatattttt aatttgtcaa aagatatgca aaaagaaact ttaagttcct ctccttcact ccaagaagaa ctcaatattt ttataaaagc agtcaatcag cgtcttatgc cattagaaca taaatggctt cgtgatgcta attatgattt gttagctgaa agcattggca tatcacaaag taaagcaaaa caaattggag agctctttat gcaaggcaag cgcctacaaa atttgttaaa agaaatcaag cgtgatcatt cagaagtaat caatatggct tgttaa
Details for BaspA.17330.d.B2.GE37048
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 28kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMY DNYIYPASHV LKNKYGIIDS DNLSMVSGHH AAKAIVNLHH EPLPERFDSF YLRYIHKRLF ENTFEWAGNS RNFPFTFNDG TTAIIQTMKK VNSDDQFLES KKIAQYLDNV DQTLAEQNNF QGLSRQAFIS KAASMFALIN YIHPFRDGNG RTQRVFFERL AEAAGHQLDF SIVTAERMRV CSILSMVRSN VMGDIGAMKH MFEDISNPEK VSIMKEFISS MSKSEYKNAQ K
Validated NT Sequence
ttttntttac tttaagaagg aganatacca tggctcacca ccaccaccac catatgtacg acaactatat ttatcctgca agccacgtac taaaaaataa atatggaata atagactcag acaatttatc catggtaagc ggacatcatg cagcgaaagc aatagttaat ttgcaccatg aaccactacc agaaaggttc gattctttct atctaagata tatccataaa cgcttgtttg agaatacatt tgaatgggct gggaatagtc gtaatttccc atttacattt aatgatggca ctactgctat tatacaaaca atgaaaaagg taaattctga cgatcagttt ttagagagta aaaaaattgc tcaataccta gataatgtgg atcaaacgct tgcagaacag aataattttc aaggcttatc gcgacaagct tttattagta aagcagcgag tatgtttgct cttattaatt atatacaccc attcagagat ggtaatggtc gcacacaacg agtgtttttt gaaagacttg ctgaagctgc aggtcatcaa ctagatttct caattgttac agcagagcgt atgagagttt gcagtatact ttctatggtg cgttctaatg ttatgggcga tataggtgct atgaagcata tgtttgaaga tatttcaaat ccagaaaaag taagtattat gaaagagttc ataagctcca tgagtaagtc tgagtataaa aatgctcaaa aataagtgag taagatagga tccggctgct aannnnnccc nnaang
Expected Protein Sequence
MAHHHHHHYD NYIYPASHVL KNKYGIIDSD NLSMVSGHHA AKAIVNLHHE PLPERFDSFY LRYIHKRLFE NTFEWAGNSR NFPFTFNDGT TAIIQTMKKV NSDDQFLESK KIAQYLDNVD QTLAEQNNFQ GLSRQAFISK AASMFALINY IHPFRDGNGR TQRVFFERLA EAAGHQLDFS IVTAERMRVC SILSMVRSNV MGDIGAMKHM FEDISNPEKV SIMKEFISSM SKSEYKNAQK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattacgaca actatattta tcctgcaagc cacgtactaa aaaataaata tggaataata gactcagaca atttatccat ggtaagcgga catcatgcag cgaaagcaat agttaatttg caccatgaac cactaccaga aaggttcgat tctttctatc taagatatat ccataaacgc ttgtttgaga atacatttga atgggctggg aatagtcgta atttcccatt tacatttaat gatggcacta ctgctattat acaaacaatg aaaaaggtaa attctgacga tcagttttta gagagtaaaa aaattgctca atacctagat aatgtggatc aaacgcttgc agaacagaat aattttcaag gcttatcgcg acaagctttt attagtaaag cagcgagtat gtttgctctt attaattata tacacccatt cagagatggt aatggtcgca cacaacgagt gttttttgaa agacttgctg aagctgcagg tcatcaacta gatttctcaa ttgttacagc agagcgtatg agagtttgca gtatactttc tatggtgcgt tctaatgtta tgggcgatat aggtgctatg aagcatatgt ttgaagatat ttcaaatcca gaaaaagtaa gtattatgaa agagttcata agctccatga gtaagtctga gtataaaaat gctcaaaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaspA.17330.d.B3.GE37049
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMY DNYIYPASHV LKNKYGIIDS DNLSMVSGHH AAKAIVNLHH EPLPERFDSF YLRYIHKRLF ENTFEWAGNS RNFPFTFNDG TTAIIQTMKK VNSDDQFLES KKIAQYLDNV DQTLAEQNNF QGLSRQAFIS KAASMFALIN YIHPFRDGNG RTQRVFFERL AEAAGHQLDF SIVTAERMRV CSILSMVRSN VMGDIGAMKH MFEDISNPEK VSIMKEFISS MSKSEYKNAQ KMIIVMPKRG HNYLSFYESE TAEHLLLXVD LNYISKPLYV IFKKDYFLXN EVKELKSGCP LSF
Validated NT Sequence
cnnnnnnnnn nttttgttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgtac gacaactata tttatcctgc aagccacgta ctaaaaaata aatatggaat aatagactca gacaatttat ccatggtaag cggacatcat gcagcgaaag caatagttaa tttgcaccat gaaccactac cagaaaggtt cgattctttc tatctaagat atatccataa acgcttgttt gagaatacat ttgaatgggc tgggaatagt cgtaatttcc catttacatt taatgatggc actactgcta ttatacaaac aatgaaaaag gtaaattctg acgatcagtt tttagagagt aaaaaaattg ctcaatacct agataatgtg gatcaaacgc ttgcagaaca gaataatttt caaggcttat cgcgacaagc ttttattagt aaagcagcga gtatgtttgc tcttattaat tatatacacc cattcagaga tggtaatggt cgcacacaac gagtgttttt tgaaagactt gctgaagctg caggtcatca actagatttc tcaattgtta cagcagagcg tatgagagtt tgcagtatac tttctatggt gcgttctaat gttatgggcg atataggtgc tatgaagcat atgtttgaag atatttcaaa tccagaaaaa gtaagtatta tgaaagagtt cataagctcc atgagtaagt ctgagtataa aaatgctcaa aaaatgatta ttgtaatgcc aaaaagaggg cataactatc taagttttta tgaaagtgag actgcagaac atcttctact cnaagtagat cttaattata tttcnaaacc gctttatgtg atattcaaaa aagattattt tttanccaat gaagttaagg aattaaagtc tggttgtccg ctaagttttt aagtgagtaa ganaggatcc ngctgctann nnnnccnaa
Expected Protein Sequence
MAHHHHHHYD NYIYPASHVL KNKYGIIDSD NLSMVSGHHA AKAIVNLHHE PLPERFDSFY LRYIHKRLFE NTFEWAGNSR NFPFTFNDGT TAIIQTMKKV NSDDQFLESK KIAQYLDNVD QTLAEQNNFQ GLSRQAFISK AASMFALINY IHPFRDGNGR TQRVFFERLA EAAGHQLDFS IVTAERMRVC SILSMVRSNV MGDIGAMKHM FEDISNPEKV SIMKEFISSM SKSEYKNAQK MIIVMPKRGH NYLSFYESET AEHLLLKVDL NYISKPLYVI FKKDYFLPNE VKELKSGCPL SF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattacgaca actatattta tcctgcaagc cacgtactaa aaaataaata tggaataata gactcagaca atttatccat ggtaagcgga catcatgcag cgaaagcaat agttaatttg caccatgaac cactaccaga aaggttcgat tctttctatc taagatatat ccataaacgc ttgtttgaga atacatttga atgggctggg aatagtcgta atttcccatt tacatttaat gatggcacta ctgctattat acaaacaatg aaaaaggtaa attctgacga tcagttttta gagagtaaaa aaattgctca atacctagat aatgtggatc aaacgcttgc agaacagaat aattttcaag gcttatcgcg acaagctttt attagtaaag cagcgagtat gtttgctctt attaattata tacacccatt cagagatggt aatggtcgca cacaacgagt gttttttgaa agacttgctg aagctgcagg tcatcaacta gatttctcaa ttgttacagc agagcgtatg agagtttgca gtatactttc tatggtgcgt tctaatgtta tgggcgatat aggtgctatg aagcatatgt ttgaagatat ttcaaatcca gaaaaagtaa gtattatgaa agagttcata agctccatga gtaagtctga gtataaaaat gctcaaaaaa tgattattgt aatgccaaaa agagggcata actatctaag tttttatgaa agtgagactg cagaacatct tctactcaaa gtagatctta attatatttc aaaaccgctt tatgtgatat tcaaaaaaga ttatttttta cccaatgaag ttaaggaatt aaagtctggt tgtccgctaa gtttttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BaspA.17330.d.B4.GE37050
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHMLI PKETVASLTS DQLIEKITSH LAVQLKRQEV DICAKYVYKN LEDFNEKISV KNILENRNFQ EVFTKRIVDF PESISKLAGT KILWLKTLKY KIAEQNVEAL AQKVHDYVDI VKEVENGIIK ENLIKKKCLM TVVAMPSKK
Validated NT Sequence
atcttactca cttatttttt gctaggcatt gctactactg tcataagaca ttttttcttt attagattct ctttaataat tccattttct acctctttta caatatcgac ataatcatga accttttgag caagtgcttc aacattttgt tcagcaattt tgtattttaa agttttcaac cataatattt ttgtgcctgc taatttagaa atagactcag gaaaatcaac tattctcttt gtaaaaactt cctgaaaatt tctattttca agaatgtttt ttacacttat cttttcatta aagtcttcta aatttttata aacatacttt gcacaaatat caacttcttg tcgctttaat tggacagcga gatgactcgt aattttttca ataagttgat ctgatgttaa agaagctacc gtttcttttg gaataagcat atggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHLI PKETVASLTS DQLIEKITSH LAVQLKRQEV DICAKYVYKN LEDFNEKISV KNILENRNFQ EVFTKRIVDF PESISKLAGT KILWLKTLKY KIAEQNVEAL AQKVHDYVDI VKEVENGIIK ENLIKKKCLM TVVAMPSKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcttattc caaaagaaac ggtagcttct ttaacatcag atcaacttat tgaaaaaatt acgagtcatc tcgctgtcca attaaagcga caagaagttg atatttgtgc aaagtatgtt tataaaaatt tagaagactt taatgaaaag ataagtgtaa aaaacattct tgaaaataga aattttcagg aagtttttac aaagagaata gttgattttc ctgagtctat ttctaaatta gcaggcacaa aaatattatg gttgaaaact ttaaaataca aaattgctga acaaaatgtt gaagcacttg ctcaaaaggt tcatgattat gtcgatattg taaaagaggt agaaaatgga attattaaag agaatctaat aaagaaaaaa tgtcttatga cagtagtagc aatgcctagc aaaaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BaspA.17330.d.B5.GE37451
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR KSKDSPSLTV EELRRRRQII DSAIHTHTIE RIPLHPDTLL VLEKYAEGGS LNEFNILMDS VIQMILQEMG NVQVEKLQSA SLSSYDNYIY PASHVLKNKY GIIDSDNLSM VSGHHAAKAI VNLHHEPLPE RFDSFYLRYI HKRLFENTFE WAGNSRNFPF TFNDGTTAII QTMKKVNSDD QFLESKKIAQ YLDNVDQTLA EQNNFQGLSR QAFISKAASM FALINYIHPF RDGNGRTQRV FFERLAEAAG HQLDFSIVTA ERMRVCSILS MVRSNVMGDI GAMKHMFEDI SNPEKVSIMK EFISSMSKSE YKNAQK
Validated NT Sequence
ctcacttatt tttgagcatt tttatactca gacttactca tggagcttat gaactctttc ataatactta ctttttctgg atttgaaata tcttcaaaca tatgcttcat agcacctata tcgcccataa cattagaacg caccatagaa agtatactgc aaactctcat acgctctgct gtaacaattg agaaatctag ttgatgacct gcagcttcag caagtctttc aaaaaacact cgttgtgtgc gaccattacc atctctgaat gggtgtatat aattaataag agcaaacata ctcgctgctt tactaataaa agcttgtcgc gataagcctt gaaaattatt ctgttctgca agcgtttgat ccacattatc taggtattga gcaatttttt tactctctaa aaactgatcg tcagaattta cctttttcat tgtttgtata atagcagtag tgccatcatt aaatgtaaat gggaaattac gactattccc agcccattca aatgtattct caaacaagcg tttatggata tatcttagat agaaagaatc gaacctttct ggtagtggtt catggtgcaa attaactatt gctttcgctg catgatgtcc gcttaccatg gataaattgt ctgagtctat tattccatat ttatttttta gtacgtggct tgcaggataa atatagttgt cgtacgatga taaagaggca gattgtagtt tttccacctg tacatttccc atttcttgca atatcatttg tataacacta tccataagga tattaaactc atttaatgaa cctccctctg catatttttc taaaactaga agtgtgtcag gatgcaaagg gatacgttca atagtatgag tatgaatcgc tgaatcaata atttggcgac gtcttcgaag ctcttctact gttagagatg gagaatcttt ggattttctc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMR KSKDSPSLTV EELRRRRQII DSAIHTHTIE RIPLHPDTLL VLEKYAEGGS LNEFNILMDS VIQMILQEMG NVQVEKLQSA SLSSYDNYIY PASHVLKNKY GIIDSDNLSM VSGHHAAKAI VNLHHEPLPE RFDSFYLRYI HKRLFENTFE WAGNSRNFPF TFNDGTTAII QTMKKVNSDD QFLESKKIAQ YLDNVDQTLA EQNNFQGLSR QAFISKAASM FALINYIHPF RDGNGRTQRV FFERLAEAAG HQLDFSIVTA ERMRVCSILS MVRSNVMGDI GAMKHMFEDI SNPEKVSIMK EFISSMSKSE YKNAQK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagaa aatccaaaga ttctccatct ctaacagtag aagagcttcg aagacgtcgc caaattattg attcagcgat tcatactcat actattgaac gtatcccttt gcatcctgac acacttctag ttttagaaaa atatgcagag ggaggttcat taaatgagtt taatatcctt atggatagtg ttatacaaat gatattgcaa gaaatgggaa atgtacaggt ggaaaaacta caatctgcct ctttatcatc gtacgacaac tatatttatc ctgcaagcca cgtactaaaa aataaatatg gaataataga ctcagacaat ttatccatgg taagcggaca tcatgcagcg aaagcaatag ttaatttgca ccatgaacca ctaccagaaa ggttcgattc tttctatcta agatatatcc ataaacgctt gtttgagaat acatttgaat gggctgggaa tagtcgtaat ttcccattta catttaatga tggcactact gctattatac aaacaatgaa aaaggtaaat tctgacgatc agtttttaga gagtaaaaaa attgctcaat acctagataa tgtggatcaa acgcttgcag aacagaataa ttttcaaggc ttatcgcgac aagcttttat tagtaaagca gcgagtatgt ttgctcttat taattatata cacccattca gagatggtaa tggtcgcaca caacgagtgt tttttgaaag acttgctgaa gctgcaggtc atcaactaga tttctcaatt gttacagcag agcgtatgag agtttgcagt atactttcta tggtgcgttc taatgttatg ggcgatatag gtgctatgaa gcatatgttt gaagatattt caaatccaga aaaagtaagt attatgaaag agttcataag ctccatgagt aagtctgagt ataaaaatgc tcaaaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at