BaspA.17330.c

Bep1 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaspA.17330.c
ORGANISM: Bartonella sp. 1-1C
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BaspA.17330.c.B1.GE35671 full length 1 553
BaspA.17330.c.B3.GE37046 FIC and OB-Fold 23 311
BaspA.17330.c.B4.GE37047 BID 322 459
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BaspA.17330.c.B4.PW37397 BID 322 459

External Resources

RESOURCE REFERENCE ID
UniProt: E6YTB2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKEILSNEAI ATETELSSNL PLPSHYTYPN SLTLKNKYGI IDHKEFTDKC AHDSAKATIN LRQEALPKEF NSFYLKYLHK RLFENTFEWA GCTRDIPFSF KDGTVAVMPE MMRSNWKTDQ PIIFATGNKV QDGLKNIDRI LVEKNNLQNL PRQEFIHHLA EIFASLNYTH PFREGNGRTQ RIFCEKLAQA ANYNLDFSIV TKERMSEVSI AAAQDGNLEP MKKLFDDISD PKKTVILKEF ISNTQNLDQE DINNCIIVTA DEGATYRGIY KGESPNSIVI KTGNTYAVCS KDYLAPEQLK TLKLNNEYTF TIPMQQNLKN TLIPKEKFPS LTKSEITKNI ITDTRIQAKL RQITHLSKIV YGNPKILNEQ IKMITNNPKV SKQLSTQIMS SPNSIANLAG IEILGIKNLA RKKAENHIVK LANSIDSYAS AVKNVETTII QEHQSEQKRR QTTVKLPSAA LQDILNLPEE KRKEFLSDKK LSSSIGKELK EFMKALNARL SPSEHKNIYE SNHQQFAKSV GISEDKAAAI IKVAQTSKEV SQQIKTPALN LEK
NT Sequence
atgaaagaaa tactctcaaa tgaagcaata gcaacagaaa cagaattatc atccaacctg cctttaccta gtcattatac ctatcccaat agtcttacac taaagaataa atatggtata atagatcata aagagtttac ggataaatgt gcccatgatt cagcaaaagc aactattaat ttgcgtcaag aagcactacc aaaagaattt aattccttct atttaaaata tcttcacaaa cgcttatttg agaatacatt tgaatgggct gggtgtaccc gcgatattcc tttttcattt aaagatggta cagtcgctgt tatgccagaa atgatgagat caaattggaa aacagaccaa ccaattattt ttgcaactgg caataaagtt caagatggtt taaaaaatat agatagaata cttgtcgaaa aaaacaattt acaaaactta ccacgccaag aatttattca tcatctagca gaaatatttg cttctctcaa ctacacgcat cctttcagag aaggcaatgg acgtacacaa cggatatttt gcgaaaaact tgctcaagct gcaaattata atcttgattt ttcaattgta acaaaagagc gtatgtctga agttagtatt gcagcagcgc aagatggcaa tctagaacct atgaaaaagc tttttgatga tatttctgat cctaaaaaaa cggttatttt aaaagaattc ataagtaata cacaaaatct tgatcaagag gatattaata actgtattat tgtaacagca gatgaaggtg caacttatag aggaatttat aaaggtgaaa gcccaaatag cattgtaatc aaaacgggca atacttacgc cgtctgtagc aaagattatt tagcaccaga acaactaaaa acattaaagc ttaataatga atacaccttt acaattccaa tgcaacaaaa tcttaaaaac actctcattc caaaagaaaa atttccctct ttaacaaaga gtgaaataac taaaaatatt ataacagata cccgtatcca agctaaacta aggcaaatta cgcatttatc aaaaattgtt tatggtaacc ccaaaatact aaatgaacag atcaagatga ttactaataa tccaaaggta agcaaacagc tttccacaca aattatgtct tctcctaatt ctattgctaa tcttgctggt atagaaatat tgggtataaa aaacttggcg cgtaaaaaag ctgaaaatca tattgtcaaa ctcgctaatt caattgacag ttatgcgtct gctgtaaaaa atgttgagac aacaataatt caagagcacc aatcagaaca aaaacgtcgt caaacaacgg taaaactacc tagtgcagca ttacaagaca ttcttaactt accagaagaa aagcggaagg aatttttatc agataaaaag ttatcttctt cgattggcaa agaactcaag gaatttatga aagcactcaa tgctcgtctt tcaccaagcg agcataaaaa tatctatgaa agtaatcatc aacaatttgc taaaagtgtt ggcatctcag aagataaagc agcagcaatc ataaaagttg ctcaaacatc taaagaagta tcccaacaaa taaaaacacc agcattgaat cttgaaaaat ag
Details for BaspA.17330.c.B1.GE35671
HARVESTED ON: 7/10/2012
SEQUENCED ON: 7/30/2012
EXPECTED MW: 63kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 55
Validated AA Sequence
MAHHHHHHMK EILSNEAIAT ETELSSNLPL PSHYTYPNSL TLKNKYGIID HKEFTDKCAH DSAKATINLR QEALPKEFNS FYLKYLHKRL FENTFEWAGC TRDIPFSFKD GTVAVMPEMM RSNWKTDQPI IFATGNKVQD GLKNIDRILV EKNNLQNLPR QEFIHHLAEI FASLNYTHPF REGNGRTQRI FCEKLAQAAN YNLDFSIVTK ERMSEVSIAA AQDGNLEPMK KLFDDISDPK KTVILKEFIS NTQNLDQEDI NNCIIVTADE GATYRGIYKG ESPNSIVIKT GNTYAVCSKD YLAPEQLKH
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa gaaatactct caaatgaagc aatagcaaca gaaacagaat tatcatccaa cctgccttta cctagtcatt atacctatcc caatagtctt acactaaaga ataaatatgg tataatagat cataaagagt ttacggataa atgtgcccat gattcagcaa aagcaactat taatttgcgt caagaagcac taccaaaaga atttaattcc ttctatttaa aatatcttca caaacgctta tttgagaata catttgaatg ggctgggtgt acccgcgata ttcctttttc atttaaagat ggtacagtcg ctgttatgcc agaaatgatg agatcaaatt ggaaaacaga ccaaccaatt atttttgcaa ctggcaataa agttcaagat ggtttaaaaa atatagatag aatacttgtc gaaaaaaaca atttacaaaa cttaccacgc caagaattta ttcatcatct agcagaaata tttgcttctc tcaactacac gcatcctttc agagaaggca atggacgtac acaacggata ttttgcgaaa aacttgctca agctgcaaat tataatcttg atttttcaat tgtaacaaaa gagcgtatgt ctgaagttag tattgcagca gcgcaagatg gcaatctaga acctatgaaa aagctttttg atgatatttc tgatcctaaa aaaacggtta ttttaaaaga attcataagt aatacacaaa atcttgatca agaggatatt aataactgta ttattgtaac agcagatgaa ggtgcaactt atagaggaat ttataaaggt gaaagcccaa atagcattgt aatcaaaacg ggcaatactt acgccgtctg tagcaaagat tatttagcac cagaacaact aaaacattaa agcttaataa tgaatacacc tttacaattc caatgcaaca aaatnntaaa aacactctca ttncaaaaga aaaatttccc tctttaacaa agagtgaaat aactaaaaat attataacag atacccgtat ccaagctaaa ntaaggcaaa ttacgcattt atcaaaaatt gtttatngta accccaaaat actaaatgaa cagatcaaga tgattactaa taatccaaag gtaagcaaac agctttccac acaaattatg tcttctccta attctattgc taatcttgct ggtatagaaa tattgggtat aaaaaacttg gcgcgtaaaa aagctgaaaa tcatattgtc aaactcgcta attcaattga cagttatgcg tctgctgtaa aaaatgttga gacaacaata attcaagagc accaatcaga acaaaaacgt cgtcaaacaa cggtaaaact acctagtgca gcattacaag acattcttaa cttaccagaa gaaaagcgga aggaattttt atcagataaa aagttatctt cttcgattgg caaagaactc aaggaattta tgaaagcact caatgctcgt ctttcaccaa gcgagcataa aaatatctat gaaagtaatc atcaacaatt tgctaaaagt gttggcatct cagaagataa agcagcagca atcataaaag ttgctcaaac atctaaagaa gtatcc
Expected Protein Sequence
MAHHHHHHMK EILSNEAIAT ETELSSNLPL PSHYTYPNSL TLKNKYGIID HKEFTDKCAH DSAKATINLR QEALPKEFNS FYLKYLHKRL FENTFEWAGC TRDIPFSFKD GTVAVMPEMM RSNWKTDQPI IFATGNKVQD GLKNIDRILV EKNNLQNLPR QEFIHHLAEI FASLNYTHPF REGNGRTQRI FCEKLAQAAN YNLDFSIVTK ERMSEVSIAA AQDGNLEPMK KLFDDISDPK KTVILKEFIS NTQNLDQEDI NNCIIVTADE GATYRGIYKG ESPNSIVIKT GNTYAVCSKD YLAPEQLKTL KLNNEYTFTI PMQQNLKNTL IPKEKFPSLT KSEITKNIIT DTRIQAKLRQ ITHLSKIVYG NPKILNEQIK MITNNPKVSK QLSTQIMSSP NSIANLAGIE ILGIKNLARK KAENHIVKLA NSIDSYASAV KNVETTIIQE HQSEQKRRQT TVKLPSAALQ DILNLPEEKR KEFLSDKKLS SSIGKELKEF MKALNARLSP SEHKNIYESN HQQFAKSVGI SEDKAAAIIK VAQTSKEVSQ QIKTPALNLE K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag aaatactctc aaatgaagca atagcaacag aaacagaatt atcatccaac ctgcctttac ctagtcatta tacctatccc aatagtctta cactaaagaa taaatatggt ataatagatc ataaagagtt tacggataaa tgtgcccatg attcagcaaa agcaactatt aatttgcgtc aagaagcact accaaaagaa tttaattcct tctatttaaa atatcttcac aaacgcttat ttgagaatac atttgaatgg gctgggtgta cccgcgatat tcctttttca tttaaagatg gtacagtcgc tgttatgcca gaaatgatga gatcaaattg gaaaacagac caaccaatta tttttgcaac tggcaataaa gttcaagatg gtttaaaaaa tatagataga atacttgtcg aaaaaaacaa tttacaaaac ttaccacgcc aagaatttat tcatcatcta gcagaaatat ttgcttctct caactacacg catcctttca gagaaggcaa tggacgtaca caacggatat tttgcgaaaa acttgctcaa gctgcaaatt ataatcttga tttttcaatt gtaacaaaag agcgtatgtc tgaagttagt attgcagcag cgcaagatgg caatctagaa cctatgaaaa agctttttga tgatatttct gatcctaaaa aaacggttat tttaaaagaa ttcataagta atacacaaaa tcttgatcaa gaggatatta ataactgtat tattgtaaca gcagatgaag gtgcaactta tagaggaatt tataaaggtg aaagcccaaa tagcattgta atcaaaacgg gcaatactta cgccgtctgt agcaaagatt atttagcacc agaacaacta aaaacattaa agcttaataa tgaatacacc tttacaattc caatgcaaca aaatcttaaa aacactctca ttccaaaaga aaaatttccc tctttaacaa agagtgaaat aactaaaaat attataacag atacccgtat ccaagctaaa ctaaggcaaa ttacgcattt atcaaaaatt gtttatggta accccaaaat actaaatgaa cagatcaaga tgattactaa taatccaaag gtaagcaaac agctttccac acaaattatg tcttctccta attctattgc taatcttgct ggtatagaaa tattgggtat aaaaaacttg gcgcgtaaaa aagctgaaaa tcatattgtc aaactcgcta attcaattga cagttatgcg tctgctgtaa aaaatgttga gacaacaata attcaagagc accaatcaga acaaaaacgt cgtcaaacaa cggtaaaact acctagtgca gcattacaag acattcttaa cttaccagaa gaaaagcgga aggaattttt atcagataaa aagttatctt cttcgattgg caaagaactc aaggaattta tgaaagcact caatgctcgt ctttcaccaa gcgagcataa aaatatctat gaaagtaatc atcaacaatt tgctaaaagt gttggcatct cagaagataa agcagcagca atcataaaag ttgctcaaac atctaaagaa gtatcccaac aaataaaaac accagcattg aatcttgaaa aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BaspA.17330.c.B3.GE37046
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MAHHHHHHMP SHYTYPNSLT LKNKYGIIDH KEFTDKCAHD SAKATINLRQ EALPKEFNSF YLKYLHKRLF ENTFEWAGCT RDIPFSFKDG TVAVMPEMMR SNWKTDQPII FATGNKVQDG LKNIDRILVE KNNLQNLPRQ EFIHHLAEIF ASLNYTHPFR EGNGRTQRIF CEKLAQAANY NLDFSIVTKE RMSEVSIAAA QDGNLEPMKK LFDDISDPKK TVILKEFISN TQNLDQEDIN NCIIVTADEG ATYRGIYKGE SPNSIVIKTG NTYAVCSKDY LAPNN
Validated NT Sequence
tttgnttnct ttaagaagga gatataccat ggctcaccac caccaccacc atatgcctag tcattatacc tatcccaata gtcttacact aaagaataaa tatggtataa tagatcataa agagtttacg gataaatgtg cccatgattc agcaaaagca actattaatt tgcgtcaaga agcactacca aaagaattta attccttcta tttaaaatat cttcacaaac gcttatttga gaatacattt gaatgggctg ggtgtacccg cgatattcct ttttcattta aagatggtac agtcgctgtt atgccagaaa tgatgagatc aaattggaaa acagaccaac caattatttt tgcaactggc aataaagttc aagatggttt aaaaaatata gatagaatac ttgtcgaaaa aaacaattta caaaacttac cacgccaaga atttattcat catctagcag aaatatttgc ttctctcaac tacacgcatc ctttcagaga aggcaatgga cgtacacaac ggatattttg cgaaaaactt gctcaagctg caaattataa tcttgatttt tcaattgtaa caaaagagcg tatgtctgaa gttagtattg cagcagcgca agatggcaat ctagaaccta tgaaaaagct ttttgatgat atttctgatc ctaaaaaaac ggttatttta aaagaattca taagtaatac acaaaatctt gatcaagagg atattaataa ctgtattatt gtaacagcag atgaaggtgc aacttataga ggaatttata aaggtgaaag cccaaatagc attgtaatca aaacgggcaa tacttacgcc gtctgtagca aagattattt agcaccgaac aactaaaaaa cattaaagct taataatgaa tacaccttta cntaagtgag taaganagga nccngctgct aacaaagccc gaanggaagc tgagttggct gctgcnancg ctgancatan ctagnataac cnnnnggggn cnctaacnnn nnnnnnnnng ngna
Expected Protein Sequence
MAHHHHHHPS HYTYPNSLTL KNKYGIIDHK EFTDKCAHDS AKATINLRQE ALPKEFNSFY LKYLHKRLFE NTFEWAGCTR DIPFSFKDGT VAVMPEMMRS NWKTDQPIIF ATGNKVQDGL KNIDRILVEK NNLQNLPRQE FIHHLAEIFA SLNYTHPFRE GNGRTQRIFC EKLAQAANYN LDFSIVTKER MSEVSIAAAQ DGNLEPMKKL FDDISDPKKT VILKEFISNT QNLDQEDINN CIIVTADEGA TYRGIYKGES PNSIVIKTGN TYAVCSKDYL APEQLKTLKL NNEYTFT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcctagtc attataccta tcccaatagt cttacactaa agaataaata tggtataata gatcataaag agtttacgga taaatgtgcc catgattcag caaaagcaac tattaatttg cgtcaagaag cactaccaaa agaatttaat tccttctatt taaaatatct tcacaaacgc ttatttgaga atacatttga atgggctggg tgtacccgcg atattccttt ttcatttaaa gatggtacag tcgctgttat gccagaaatg atgagatcaa attggaaaac agaccaacca attatttttg caactggcaa taaagttcaa gatggtttaa aaaatataga tagaatactt gtcgaaaaaa acaatttaca aaacttacca cgccaagaat ttattcatca tctagcagaa atatttgctt ctctcaacta cacgcatcct ttcagagaag gcaatggacg tacacaacgg atattttgcg aaaaacttgc tcaagctgca aattataatc ttgatttttc aattgtaaca aaagagcgta tgtctgaagt tagtattgca gcagcgcaag atggcaatct agaacctatg aaaaagcttt ttgatgatat ttctgatcct aaaaaaacgg ttattttaaa agaattcata agtaatacac aaaatcttga tcaagaggat attaataact gtattattgt aacagcagat gaaggtgcaa cttatagagg aatttataaa ggtgaaagcc caaatagcat tgtaatcaaa acgggcaata cttacgccgt ctgtagcaaa gattatttag caccagaaca actaaaaaca ttaaagctta ataatgaata cacctttaca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BaspA.17330.c.B4.GE37047
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 16kDa
OBSERVED MW: 15kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MLIPKEKFPS LTKSEITKNI ITDTRIQAKL RQITHLSKIV YGNPKILNEQ IKMITNNPKV SKQLSTQIMS SPNSIANLAG IEILGIKNLA RKKAENHIVK LANSIDSYAS AVKNVETTII QEHQSEQKRR QTTVKLPSA
Validated NT Sequence
atcttactca cttatgcact aggtagtttt accgttgttt gacgacgttt ttgttctgat tggtgctctt gaattattgt tgtctcaaca ttttttacag cagacgcata actgtcaatt gaattagcga gtttgacaat atgattttca gcttttttac gcgccaagtt ttttataccc aatatttcta taccagcaag attagcaata gaattaggag aagacataat ttgtgtggaa agctgtttgc ttacctttgg attattagta atcatcttga tctgttcatt tagtattttg gggttaccat aaacaatttt tgataaatgc gtaatttgcc ttagtttagc ttggatacgg gtatctgtta taatattttt agttatttca ctctttgtta aagagggaaa tttttctttt ggaatgagca tatggtggtg gtggtggtga gttat
Expected Protein Sequence
MAHHHHHHLI PKEKFPSLTK SEITKNIITD TRIQAKLRQI THLSKIVYGN PKILNEQIKM ITNNPKVSKQ LSTQIMSSPN SIANLAGIEI LGIKNLARKK AENHIVKLAN SIDSYASAVK NVETTIIQEH QSEQKRRQTT VKLPSA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctcattc caaaagaaaa atttccctct ttaacaaaga gtgaaataac taaaaatatt ataacagata cccgtatcca agctaaacta aggcaaatta cgcatttatc aaaaattgtt tatggtaacc ccaaaatact aaatgaacag atcaagatga ttactaataa tccaaaggta agcaaacagc tttccacaca aattatgtct tctcctaatt ctattgctaa tcttgctggt atagaaatat tgggtataaa aaacttggcg cgtaaaaaag ctgaaaatca tattgtcaaa ctcgctaatt caattgacag ttatgcgtct gctgtaaaaa atgttgagac aacaataatt caagagcacc aatcagaaca aaaacgtcgt caaacaacgg taaaactacc tagtgcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BaspA.17330.c.B4.PW37397
PURIFICATION DATe: 11/18/2013
CONCENTRATION: 31.4mg/ml
OBSERVED MW: 16kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MLIPKEKFPS LTKSEITKNI ITDTRIQAKL RQITHLSKIV YGNPKILNEQ IKMITNNPKV SKQLSTQIMS SPNSIANLAG IEILGIKNLA RKKAENHIVK LANSIDSYAS AVKNVETTII QEHQSEQKRR QTTVKLPSA
Validated NT Sequence
atcttactca cttatgcact aggtagtttt accgttgttt gacgacgttt ttgttctgat tggtgctctt gaattattgt tgtctcaaca ttttttacag cagacgcata actgtcaatt gaattagcga gtttgacaat atgattttca gcttttttac gcgccaagtt ttttataccc aatatttcta taccagcaag attagcaata gaattaggag aagacataat ttgtgtggaa agctgtttgc ttacctttgg attattagta atcatcttga tctgttcatt tagtattttg gggttaccat aaacaatttt tgataaatgc gtaatttgcc ttagtttagc ttggatacgg gtatctgtta taatattttt agttatttca ctctttgtta aagagggaaa tttttctttt ggaatgagca tatggtggtg gtggtggtga gttat
Expressed Protein Sequence
MAHHHHHHLI PKEKFPSLTK SEITKNIITD TRIQAKLRQI THLSKIVYGN PKILNEQIKM ITNNPKVSKQ LSTQIMSSPN SIANLAGIEI LGIKNLARKK AENHIVKLAN SIDSYASAVK NVETTIIQEH QSEQKRRQTT VKLPSA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTCATTC CAAAAGAAAA ATTTCCCTCT TTAACAAAGA GTGAAATAAC TAAAAATATT ATAACAGATA CCCGTATCCA AGCTAAACTA AGGCAAATTA CGCATTTATC AAAAATTGTT TATGGTAACC CCAAAATACT AAATGAACAG ATCAAGATGA TTACTAATAA TCCAAAGGTA AGCAAACAGC TTTCCACACA AATTATGTCT TCTCCTAATT CTATTGCTAA TCTTGCTGGT ATAGAAATAT TGGGTATAAA AAACTTGGCG CGTAAAAAAG CTGAAAATCA TATTGTCAAA CTCGCTAATT CAATTGACAG TTATGCGTCT GCTGTAAAAA ATGTTGAGAC AACAATAATT CAAGAGCACC AATCAGAACA AAAACGTCGT CAAACAACGG TAAAACTACC TAGTGCATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT