BaspA.01664.a

Two-component sensor kinase BatS (EC 2.7.3.-) (BatR-BatS two-component system)

CENTER ID: BaspA.01664.a
ORGANISM: Bartonella sp. 1-1C
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaspA.01664.a.B3.GE37276 periplasmic domain 29 240
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: E6YTA5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFFSSLTRRI VILNLAALGI LVTSILYLNQ FRNGLIEAKI ESLRTQGKII AGAIAASATI DTNSILIDPQ KLLELQAGES VTPMLQSTDT WDFPINSEQV APLLRRLITP KTTRARIYDR DATLLLDSRV LYSSGEVFTY DLPPIQTKKN LWEHLKSLFF NTLYGNGIAV DRRQAKDNET LHPEVYLALN GSSATAKRRN RQGQLIVSVA VPVQRYRAVV GALLLSTIGT DIDDIVESER LGIFKIFAVV GSVFLVLSLF LAHTIAHPLS KLSSSANRVR HGRSKRITIP DFSKREDEIG RLSTSIRDMT NALYTRIEAI ERFAADVSHE LKNPLTSLRS AVETLPLAKN KAQQTQLLEI IQHDVHRLDR LITDISDASR LDAELVRETA KIVDMGSLLK SLIQAIREIH CNKQNIDINL NIIPRTNGKP YLIVGHELRL GQVISNLIEN ARSFVPRKNG KICITMKNNA STLILTVEDN GPGIRSENIE RIFERFYTDR SGKDAFGKHS GLGLAISQQI IEAHNGTLMA ENIIDSKSKK EKIGARFIIT LPLTK
NT Sequence
ttgttctttt ccagtctcac acgtcgtatt gtgattttaa atctcgctgc ccttggtatt ctagttacaa gtattttgta tcttaaccaa ttccgtaatg gtttgattga agcaaaaatt gaaagtttac gcacccaagg aaaaatcatc gctggagcta ttgctgcttc tgcaacaata gacacaaact ctattttaat tgaccctcaa aaacttttgg aattacaagc tggagaaagt gttacaccaa tgcttcaatc aactgatact tgggattttc ccattaattc cgaacaagtt gctccacttt tacgacgtct tattacacca aaaacaacaa gagcacggat atatgatcgt gatgcgactt tgcttcttga ttcacgtgtt ctttattcga gtggtgaagt ttttacctat gatttacctc ctattcaaac aaaaaaaaat ttatgggaac acttaaaatc attgtttttt aatacactct atggcaatgg tatcgctgtt gatagaagac aagcaaaaga taatgaaact cttcatcctg aagtttatct agcattaaat ggctcttctg ctactgctaa acgacgtaac aggcaaggtc aactaattgt ttcagttgct gtacctgttc aacgttatcg tgcagtcgtt ggtgctcttt tgctttcaac tattggtaca gacattgatg atattgtaga aagtgaaaga ttaggaattt ttaaaatctt tgccgttgtt ggaagtgttt ttttggttct ttctctattt ttagctcata ctattgcgca tccactaagt aaattatctt cctccgctaa tcgtgtgcgt catggtcgca gtaagagaat aacaattcct gatttttcaa aacgtgaaga tgaaattggt cgtctttcta cctctattcg tgatatgacc aacgctcttt atacacgcat tgaagccatt gaacgttttg cagccgatgt aagtcatgaa ttaaagaatc ctcttacctc gctgcgcagt gccgttgaaa cattaccact agctaaaaat aaagcacaac agacacaatt acttgaaatt atccaacatg acgtgcatcg tcttgatcgt ttaattaccg atatttctga cgcatcgcga ttagatgcag aacttgttcg cgaaactgct aaaatagttg atatgggttc attgttaaaa agccttattc aagctattcg tgaaatccat tgcaataaac aaaatattga tattaatttg aatattattc cacgaactaa tggcaaacct tacctaattg taggacatga attacgttta ggccaagtga tttcaaatct cattgaaaat gcacgttctt ttgtacctcg taaaaatggt aaaatttgta ttacaatgaa aaataatgct tcaactctca ttttaactgt cgaagacaat ggcccaggta ttcgttcaga aaatattgaa cgtatttttg aacgtttcta tactgatcgg tcaggcaaag atgcttttgg gaaacattca ggtcttggcc ttgcaattag tcagcaaatc attgaagctc ataatggaac tctcatggct gaaaatatta ttgattccaa atctaaaaaa gaaaaaattg gtgctcgatt tattatcact cttccactca ccaaataa
Details for BaspA.01664.a.B3.GE37276
HARVESTED ON: 8/28/2013
SEQUENCED ON: 9/3/2013
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMN QFRNGLIEAK IESLRTQGKI IAGAIAASAT IDTNSILIDP QKLLELQAGE SVTPMLQSTD TWDFPINSEQ VAPLLRRLIT PKTTRARIYD RDATLLLDSR VLYSSGEVFT YDLPPIQTKK NLWEHLKSLF FNTLYGNGIA VDRRQAKDNE TLHPEVYLAL NGSSATAKRR NRQGQLIVSV AVPVQRYRAV VGALLLSTIG TDIDEL
Validated NT Sequence
attttnttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgaac caattccgta atggtttgat tgaagcaaaa attgaaagtt tacgcaccca aggaaaaatc atcgctggag ctattgctgc ttctgcaaca atagacacaa actctatttt aattgaccct caaaaacttt tggaattaca agctggagaa agtgttacac caatgcttca atcaactgat acttgggatt ttcccattaa ttccgaacaa gttgctccac ttttacgacg tcttattaca ccaaaaacaa caagagcacg gatatatgat cgtgatgcga ctttgcttct tgattcacgt gttctttatt cgagtggtga agtttttacc tatgatttac ctcctattca aacaaaaaaa aatttatggg aacacttaaa atcattgttt tttaatacac tctatggcaa tggtatcgct gttgatagaa gacaagcaaa agataatgaa actcttcatc ctgaagttta tctagcatta aatggctctt ctgctactgc taaacgacgt aacaggcaag gtcaactaat tgtttcagtt gctgtacctg ttcaacgtta tcgtgcagtc gttggtgctc ttttgctttc aactattggt acagacattg atgaattgta gaaagtgaaa gataagtgag taagatagga tccggctgct aacnnncccc cnaanannnn nnnnnannna nnnnnnnnnn nnnnntnnnn nnnnnncnnn nntnnnnnnn ntcnnnnttn cnnnnngacc nnnnnncc
Expected Protein Sequence
MAHHHHHHNQ FRNGLIEAKI ESLRTQGKII AGAIAASATI DTNSILIDPQ KLLELQAGES VTPMLQSTDT WDFPINSEQV APLLRRLITP KTTRARIYDR DATLLLDSRV LYSSGEVFTY DLPPIQTKKN LWEHLKSLFF NTLYGNGIAV DRRQAKDNET LHPEVYLALN GSSATAKRRN RQGQLIVSVA VPVQRYRAVV GALLLSTIGT DIDDIVESER
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaccaat tccgtaatgg tttgattgaa gcaaaaattg aaagtttacg cacccaagga aaaatcatcg ctggagctat tgctgcttct gcaacaatag acacaaactc tattttaatt gaccctcaaa aacttttgga attacaagct ggagaaagtg ttacaccaat gcttcaatca actgatactt gggattttcc cattaattcc gaacaagttg ctccactttt acgacgtctt attacaccaa aaacaacaag agcacggata tatgatcgtg atgcgacttt gcttcttgat tcacgtgttc tttattcgag tggtgaagtt tttacctatg atttacctcc tattcaaaca aaaaaaaatt tatgggaaca cttaaaatca ttgtttttta atacactcta tggcaatggt atcgctgttg atagaagaca agcaaaagat aatgaaactc ttcatcctga agtttatcta gcattaaatg gctcttctgc tactgctaaa cgacgtaaca ggcaaggtca actaattgtt tcagttgctg tacctgttca acgttatcgt gcagtcgttg gtgctctttt gctttcaact attggtacag acattgatga tattgtagaa agtgaaagat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat