BasaA.18418.a

Effector protein yopJ (Modular protein) (Yop-like family protein)

CENTER ID: BasaA.18418.a
ORGANISM: Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BasaA.18418.a.B1.GE35668 full length 1 401
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BasaA.18418.a.B1.PS01657 full length 1 401

External Resources

RESOURCE REFERENCE ID
UniProt: E6YPL1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPRSKNLTDQ DSGPIQTSSP LNSSATLEEL LTHLETLETQ ISDPSQTNDP SQASGLNQAS GFNQASGPIQ TSSPLNSSAT LEELLTRLGP LKAQASGLNQ DSGPIQTSSP LNSSATLEEL LSRLEANETT EKPSILFSKK ALQNVISDLE NDITNSSFVD ANYAHIDLTI MPHLVEQENN KCPGINLQFI TDPEQLVKSI QKVINQGIPS SRFLISLEGN DPHFAIIDQQ TIANKTSLIL FETTKFTHMN AEVLGVTVKS AIEAAQLPYC YFSMAEINLQ ANSSESGIIS LLFAKKLHLE ANKLKVMHQD NISGILCKRG IPLPYHKVDE YLPGAFYKHA QTGKRISQYV DKNRQYFTKT INNKDEILHH RLDKHSVLLD NKVVSCSSQK KRIEKYKALV R
NT Sequence
atgcctagat caaaaaattt aacagaccaa gacagtggtc caattcaaac cagtagtcca ttaaatagta gtgcaacatt agaagagctt ctaacccatt tagagacttt ggagacgcaa atcagtgatc caagccaaac caacgatcca agccaagcca gcggtctcaa ccaagccagt ggtttcaacc aagccagtgg tccaattcaa accagtagtc cattaaatag tagtgcaaca ttagaagagc ttctaacgcg tttaggacct ttgaaggctc aagccagcgg tctcaaccaa gacagtggtc caattcaaac cagtagtcca ttaaatagta gtgcaacatt agaagagctt ctatcgcgtt tagaggctaa tgaaacaaca gaaaaaccaa gtatcttatt cagtaagaaa gcattacaaa atgttatttc agatttagaa aatgacatta ctaatagtag tttcgtcgat gctaattatg cacatataga ccttacaata atgcctcact tagtcgagca ggaaaataat aaatgtccag ggataaatct tcaatttatt acagaccctg aacagcttgt gaaatcaata caaaaagtaa ttaatcaggg aattccatct tctagatttc taattagtct agaaggtaat gaccctcatt ttgcgataat tgatcaacaa accattgcta ataaaacatc cttaatattg tttgaaacta caaaattcac gcacatgaat gcagaagtac tgggagtgac cgtgaaatca gccattgaag ctgcacaact accttattgt tatttttcca tggcggaaat taatcttcaa gcaaactcct ctgaaagtgg aattataagt ttactttttg ccaaaaaact tcacctcgaa gctaataaat taaaagtaat gcaccaagat aatattagtg ggattttatg taaacgagga attcctttac cttatcataa agtggatgag tatcttccag gcgcttttta taaacatgca caaactggaa aacgtattag tcaatatgtc gacaaaaatc ggcagtattt tactaaaaca attaacaaca aagatgaaat tttgcatcat agattagata aacactcagt gttattagac aataaggttg tatcttgttc atcacaaaag aaaagaattg agaaatataa agctctggtc agataa
Details for BasaA.18418.a.B1.GE35668
HARVESTED ON: 7/10/2012
SEQUENCED ON: 7/31/2012
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMP RSKNLTDQDS GPIQTSSPLN SSATLEELLT HLETLETQIS DPSQTNDPSQ ASGLNQASGF NQASGPIQTS SPLNSSATLE ELLTRLGPLK AQASGLNQDS GPIQTSSPLN SSATLEELLS RLEANETTEK PSILFSKKAL QNVISDLEND ITNSSFVDAN YAHIDLTIMP HLVEQENNKC PGINLQFITD PEQLVKSIQK VINQGIPSSR FLISLEGNDP HFAIIDQQTI ANKTSLILFE TTKFTHMNAE VLGVTVKSAI EAAQLPYCYF SMAEINLQAN SSESGIISLL FAKKLHLEAN KLKVMHQDNI SGILCKRGIP LPYHKVDEYL PGAFYKHAQT GKRISQYVDK NRQYFTKTIN NKDEILHHRL DKHSVLLDNK VVSCSSQKKR
Validated NT Sequence
ataccatggc tcaccaccac caccaccata tgcctagatc aaaaaattta acagaccaag acagtggtcc aattcaaacc agtagtccat taaatagtag tgcaacatta gaagagcttc taacccattt agagactttg gagacgcaaa tcagtgatcc aagccaaacc aacgatccaa gccaagccag cggtctcaac caagccagtg gtttcaacca agccagtggt ccaattcaaa ccagtagtcc attaaatagt agtgcaacat tagaagagct tctaacgcgt ttaggacctt tgaaggctca agccagcggt ctcaaccaag acagtggtcc aattcaaacc agtagtccat taaatagtag tgcaacatta gaagagcttc tatcgcgttt agaggctaat gaaacaacag aaaaaccaag tatcttattc agtaagaaag cattacaaaa tgttatttca gatttagaaa atgacattac taatagtagt ttcgtcgatg ctaattatgc acatatagac cttacaataa tgcctcactt agtcgagcag gaaaataata aatgtccagg gataaatctt caatttatta cagaccctga acagcttgtg aaatcaatac aaaaagtaat taatcaggga attccatctt ctagatttct aattagtcta gaaggtaatg accctcattt tgcgataatt gatcaacaaa ccattgctaa taaaacatcc ttaatattgt ttgaaactac aaaattcacg cacatgaatg cagaagtact gggagtgacc gtgaaatcag ccattgaagc tgcacaacta ccttattgtt atttttccat ggcggaaatt aatcttcaag caaactcctc tgaaagtgga attataagtt tactttttgc caaaaaactt cacctcgaag ctaataaatt aaaagtaatg caccaagata atattagtgg gattttatgt aaacgaggaa ttcctttacc ttatcataaa gtggatgagt atcttccagg cgctttttat aaacatgcac aaactggaaa acgtattagt caatatgtcg acaaaaatcg gcagtatttt actaaaacaa ttaacaacaa agatgaaatt ttgcatcata gattagataa acactcagtg ttattagaca ataaggttgt atcttgttca tcacaaaaga aaagaat
Expected Protein Sequence
MAHHHHHHMP RSKNLTDQDS GPIQTSSPLN SSATLEELLT HLETLETQIS DPSQTNDPSQ ASGLNQASGF NQASGPIQTS SPLNSSATLE ELLTRLGPLK AQASGLNQDS GPIQTSSPLN SSATLEELLS RLEANETTEK PSILFSKKAL QNVISDLEND ITNSSFVDAN YAHIDLTIMP HLVEQENNKC PGINLQFITD PEQLVKSIQK VINQGIPSSR FLISLEGNDP HFAIIDQQTI ANKTSLILFE TTKFTHMNAE VLGVTVKSAI EAAQLPYCYF SMAEINLQAN SSESGIISLL FAKKLHLEAN KLKVMHQDNI SGILCKRGIP LPYHKVDEYL PGAFYKHAQT GKRISQYVDK NRQYFTKTIN NKDEILHHRL DKHSVLLDNK VVSCSSQKKR IEKYKALVR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccta gatcaaaaaa tttaacagac caagacagtg gtccaattca aaccagtagt ccattaaata gtagtgcaac attagaagag cttctaaccc atttagagac tttggagacg caaatcagtg atccaagcca aaccaacgat ccaagccaag ccagcggtct caaccaagcc agtggtttca accaagccag tggtccaatt caaaccagta gtccattaaa tagtagtgca acattagaag agcttctaac gcgtttagga cctttgaagg ctcaagccag cggtctcaac caagacagtg gtccaattca aaccagtagt ccattaaata gtagtgcaac attagaagag cttctatcgc gtttagaggc taatgaaaca acagaaaaac caagtatctt attcagtaag aaagcattac aaaatgttat ttcagattta gaaaatgaca ttactaatag tagtttcgtc gatgctaatt atgcacatat agaccttaca ataatgcctc acttagtcga gcaggaaaat aataaatgtc cagggataaa tcttcaattt attacagacc ctgaacagct tgtgaaatca atacaaaaag taattaatca gggaattcca tcttctagat ttctaattag tctagaaggt aatgaccctc attttgcgat aattgatcaa caaaccattg ctaataaaac atccttaata ttgtttgaaa ctacaaaatt cacgcacatg aatgcagaag tactgggagt gaccgtgaaa tcagccattg aagctgcaca actaccttat tgttattttt ccatggcgga aattaatctt caagcaaact cctctgaaag tggaattata agtttacttt ttgccaaaaa acttcacctc gaagctaata aattaaaagt aatgcaccaa gataatatta gtgggatttt atgtaaacga ggaattcctt taccttatca taaagtggat gagtatcttc caggcgcttt ttataaacat gcacaaactg gaaaacgtat tagtcaatat gtcgacaaaa atcggcagta ttttactaaa acaattaaca acaaagatga aattttgcat catagattag ataaacactc agtgttatta gacaataagg ttgtatcttg ttcatcacaa aagaaaagaa ttgagaaata taaagctctg gtcagatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BasaA.18418.a.B1.PS01657
PURIFICATION DATe: 10/16/2012
CONCENTRATION: 31mg/ml
OBSERVED MW: 43kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP RSKNLTDQDS GPIQTSSPLN SSATLEELLT HLETLETQIS DPSQTNDPSQ ASGLNQASGF NQASGPIQTS SPLNSSATLE ELLTRLGPLK AQASGLNQDS GPIQTSSPLN SSATLEELLS RLEANETTEK PSILFSKKAL QNVISDLEND ITNSSFVDAN YAHIDLTIMP HLVEQENNKC PGINLQFITD PEQLVKSIQK VINQGIPSSR FLISLEGNDP HFAIIDQQTI ANKTSLILFE TTKFTHMNAE VLGVTVKSAI EAAQLPYCYF SMAEINLQAN SSESGIISLL FAKKLHLEAN KLKVMHQDNI SGILCKRGIP LPYHKVDEYL PGAFYKHAQT GKRISQYVDK NRQYFTKTIN NKDEILHHRL DKHSVLLDNK VVSCSSQKKR
Validated NT Sequence
ataccatggc tcaccaccac caccaccata tgcctagatc aaaaaattta acagaccaag acagtggtcc aattcaaacc agtagtccat taaatagtag tgcaacatta gaagagcttc taacccattt agagactttg gagacgcaaa tcagtgatcc aagccaaacc aacgatccaa gccaagccag cggtctcaac caagccagtg gtttcaacca agccagtggt ccaattcaaa ccagtagtcc attaaatagt agtgcaacat tagaagagct tctaacgcgt ttaggacctt tgaaggctca agccagcggt ctcaaccaag acagtggtcc aattcaaacc agtagtccat taaatagtag tgcaacatta gaagagcttc tatcgcgttt agaggctaat gaaacaacag aaaaaccaag tatcttattc agtaagaaag cattacaaaa tgttatttca gatttagaaa atgacattac taatagtagt ttcgtcgatg ctaattatgc acatatagac cttacaataa tgcctcactt agtcgagcag gaaaataata aatgtccagg gataaatctt caatttatta cagaccctga acagcttgtg aaatcaatac aaaaagtaat taatcaggga attccatctt ctagatttct aattagtcta gaaggtaatg accctcattt tgcgataatt gatcaacaaa ccattgctaa taaaacatcc ttaatattgt ttgaaactac aaaattcacg cacatgaatg cagaagtact gggagtgacc gtgaaatcag ccattgaagc tgcacaacta ccttattgtt atttttccat ggcggaaatt aatcttcaag caaactcctc tgaaagtgga attataagtt tactttttgc caaaaaactt cacctcgaag ctaataaatt aaaagtaatg caccaagata atattagtgg gattttatgt aaacgaggaa ttcctttacc ttatcataaa gtggatgagt atcttccagg cgctttttat aaacatgcac aaactggaaa acgtattagt caatatgtcg acaaaaatcg gcagtatttt actaaaacaa ttaacaacaa agatgaaatt ttgcatcata gattagataa acactcagtg ttattagaca ataaggttgt atcttgttca tcacaaaaga aaagaat
Expressed Protein Sequence
MAHHHHHHMP RSKNLTDQDS GPIQTSSPLN SSATLEELLT HLETLETQIS DPSQTNDPSQ ASGLNQASGF NQASGPIQTS SPLNSSATLE ELLTRLGPLK AQASGLNQDS GPIQTSSPLN SSATLEELLS RLEANETTEK PSILFSKKAL QNVISDLEND ITNSSFVDAN YAHIDLTIMP HLVEQENNKC PGINLQFITD PEQLVKSIQK VINQGIPSSR FLISLEGNDP HFAIIDQQTI ANKTSLILFE TTKFTHMNAE VLGVTVKSAI EAAQLPYCYF SMAEINLQAN SSESGIISLL FAKKLHLEAN KLKVMHQDNI SGILCKRGIP LPYHKVDEYL PGAFYKHAQT GKRISQYVDK NRQYFTKTIN NKDEILHHRL DKHSVLLDNK VVSCSSQKKR IEKYKALVR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCTA GATCAAAAAA TTTAACAGAC CAAGACAGTG GTCCAATTCA AACCAGTAGT CCATTAAATA GTAGTGCAAC ATTAGAAGAG CTTCTAACCC ATTTAGAGAC TTTGGAGACG CAAATCAGTG ATCCAAGCCA AACCAACGAT CCAAGCCAAG CCAGCGGTCT CAACCAAGCC AGTGGTTTCA ACCAAGCCAG TGGTCCAATT CAAACCAGTA GTCCATTAAA TAGTAGTGCA ACATTAGAAG AGCTTCTAAC GCGTTTAGGA CCTTTGAAGG CTCAAGCCAG CGGTCTCAAC CAAGACAGTG GTCCAATTCA AACCAGTAGT CCATTAAATA GTAGTGCAAC ATTAGAAGAG CTTCTATCGC GTTTAGAGGC TAATGAAACA ACAGAAAAAC CAAGTATCTT ATTCAGTAAG AAAGCATTAC AAAATGTTAT TTCAGATTTA GAAAATGACA TTACTAATAG TAGTTTCGTC GATGCTAATT ATGCACATAT AGACCTTACA ATAATGCCTC ACTTAGTCGA GCAGGAAAAT AATAAATGTC CAGGGATAAA TCTTCAATTT ATTACAGACC CTGAACAGCT TGTGAAATCA ATACAAAAAG TAATTAATCA GGGAATTCCA TCTTCTAGAT TTCTAATTAG TCTAGAAGGT AATGACCCTC ATTTTGCGAT AATTGATCAA CAAACCATTG CTAATAAAAC ATCCTTAATA TTGTTTGAAA CTACAAAATT CACGCACATG AATGCAGAAG TACTGGGAGT GACCGTGAAA TCAGCCATTG AAGCTGCACA ACTACCTTAT TGTTATTTTT CCATGGCGGA AATTAATCTT CAAGCAAACT CCTCTGAAAG TGGAATTATA AGTTTACTTT TTGCCAAAAA ACTTCACCTC GAAGCTAATA AATTAAAAGT AATGCACCAA GATAATATTA GTGGGATTTT ATGTAAACGA GGAATTCCTT TACCTTATCA TAAAGTGGAT GAGTATCTTC CAGGCGCTTT TTATAAACAT GCACAAACTG GAAAACGTAT TAGTCAATAT GTCGACAAAA ATCGGCAGTA TTTTACTAAA ACAATTAACA ACAAAGATGA AATTTTGCAT CATAGATTAG ATAAACACTC AGTGTTATTA GACAATAAGG TTGTATCTTG TTCATCACAA AAGAAAAGAA TTGAGAAATA TAAAGCTCTG GTCAGATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T