SSGCID: Target Page

BasaA.18410.a

Bep9 protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BasaA.18410.a
ORGANISM:	Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BasaA.18410.a.B1.GE35667	full length		1	756
BasaA.18410.a.B2.GE37360	BID1		14	147

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	E6YS54

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTLANKTEGF GHFLIPPKQL SISVPDTSQD KNIDGYFSAK KRAVVKTLSA IVFRDGRALD RAIDRVTEHP GSVYALAKMI TEKPETFGKL AGSIKLGLRT PSRIRAEKNI QILADEIVEY AQMINALSNN YFEPMQRSTQ AVEMLSDDVL HLLNLPNDIR RHTLEQQDTS KVQKELSYFL DEMAFRLSEN ENGMLRSNNY KMLAKSLDIS KHKAQEIINI VRQVESLQLE IQEINKNRFS QEKHAEVIEN DQNIYESYDP QTQSSESLLN PSKEINPTKV IDSIYDNPSK EVDPVYANYT PNKTQTQNSR SFLNSAKKVD PVYDDPSKEI NPTKVIDSIY DNPSKEVDPV YANYTPNKTQ TQNSRSFLNS AKKIDPVYDD PSKEINPTKV IDSIYDNPSK EVDPVYANYT PNKTQTQNSR SFLNSAKKVD PVYDDPSKEI NPTKVIDSIY DNPSKEVDPV YANYTPNKTQ TQNSRSFLNS AKKIDPVYDD PSKEIDSVYA NYIPNKTQTQ NSRSFLIPPQ ELGPLTQNEI INKIFDNIIV QRHIGEIKHL SGIVFGNKEV FNSVLTNIIE TKKPEEIGEG YIKVLEVAPK NFAKLAGIKM WGLKNPRYKQ AKENVPELCK AIKDYTNVVK KYKEQILSHH KEEQKRVKQA VLMPNNEIQR FLNMPSDMLE NILIKQNISQ LHKEFSNFLN QVHSRLTAQE RKAIKRNDYH TLARNIKVPE VKAREIINVV KKAQECQQKI KDIISQKQKQ MIAVAH

NT Sequence

atgacacttg caaataaaac agaaggattt gggcattttt taattcctcc aaaacaatta tctatttctg tgcccgatac atcacaggac aaaaacatag atggttattt ttctgcaaag aagagagctg tagttaaaac tttgtcagcg attgtgttta gagacgggag ggctttagat agggcaatag atcgggttac tgaacatcca ggttctgtgt atgcattggc caaaatgata acagaaaaac ctgaaacttt cggtaaactt gcaggttcta ttaaattggg actaagaact ccgagccgta tacgagctga aaaaaatatt caaatcctgg ctgatgaaat tgtagaatat gcgcagatga taaacgcatt atcaaataat tattttgaac caatgcaacg ctcaacacaa gcagtcgaaa tgttgagtga cgatgtgctg catctgctta atctaccaaa tgacatacgg cgacatactt tagaacagca ggatacttct aaagttcaga aagaactttc gtatttttta gatgagatgg cttttcgtct ttcagaaaac gagaatggaa tgcttagaag caataattat aaaatgcttg ctaagagcct ggatatatca aaacataaag cacaggaaat cataaatatt gtacggcaag tggaaagcct gcaactagag attcaagaga ttaacaaaaa cagattttct caagaaaaac atgcagaggt catcgaaaac gatcagaata tttatgagtc atatgaccct caaacgcaga gttctgaaag tcttcttaat ccttcaaaag aaataaatcc tacaaaagta atcgattcta tctatgataa tccttcaaaa gaagtagatc ctgtctatgc aaattatacc ccaaacaaaa ctcaaacgca gaattctaga agttttctta attctgcaaa aaaagtagat cctgtctatg acgatccttc aaaagaaata aatcctacaa aagtaatcga ttctatctat gataatcctt caaaagaagt agatcctgtc tatgcaaatt ataccccaaa caaaactcaa acgcagaatt ctagaagttt tcttaattct gcaaaaaaaa tagatcctgt ctatgacgat ccttcaaaag aaataaatcc tacaaaagta atcgattcta tctatgataa tccttcaaaa gaagtagatc ctgtctatgc aaattatacc ccaaacaaaa ctcaaacgca gaattctaga agttttctta attctgcaaa aaaagtagat cctgtctatg acgatccttc aaaagaaata aatcctacaa aagtaatcga ttctatctat gataatcctt caaaagaagt agatcctgtc tatgcaaatt ataccccaaa caaaactcaa acgcagaatt ctagaagttt tcttaattct gcaaaaaaaa tagatcctgt ctatgacgat ccttcaaaag aaatagattc tgtctatgca aattatatcc caaacaaaac tcaaacgcag aattctagaa gttttcttat tcctccacaa gaattaggtc ctctaactca gaatgaaatt attaataaaa tttttgataa tattattgtt caaaggcata taggagaaat taagcatctt tcaggaattg tatttgggaa taaagaagta tttaacagcg ttctaacaaa catcattgag acaaaaaaac cagaagaaat cggagaaggg tatatcaaag tactagaagt tgctcctaaa aactttgcta aacttgctgg tattaaaatg tgggggctaa aaaatccgag atataaacaa gctaaagaaa atgtcccaga actctgtaaa gcaattaaag attatacaaa tgttgtaaaa aaatataagg aacaaattct ttcccatcat aaagaagaac aaaaacgtgt caaacaagcg gtattaatgc ctaataatga aatacagcgt tttttaaata tgccaagtga tatgttggaa aatattttaa taaagcaaaa tatttctcaa cttcataaag aattttctaa ttttttaaat caagttcatt ctcgtcttac agcacaagaa cgtaaagcga ttaagcgtaa tgattatcat acgctcgctc gcaatattaa ggtaccagaa gttaaagcac gtgagattat aaatgtcgta aaaaaagcac aagaatgcca acaaaaaatt aaagatataa tatctcagaa acagaaacaa atgatagctg tagctcatta a

Details for BasaA.18410.a.B1.GE35667

HARVESTED ON:	7/10/2012
SEQUENCED ON:	2/22/2016
EXPECTED MW:	87kDa
OBSERVED MW:	87kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	41

Validated AA Sequence

Validated NT Sequence

atggctcacc accaccacca ccatatgaca cttgcaaata aaacagaagg atttgggcat tttttaattc ctccaaaaca attatctatt tctgtgcccg atacatcaca ggacaaaaac atagatggtt atttttctgc aaagaagaga gctgtagtta aaactttgtc agcgattgtg tttagagacg ggagggcttt agatagggca atagatcggg ttactgaaca tccaggttct gtgtatgcat tggccaaaat gataacagaa aaacctgaaa ctttcggtaa acttgcaggt tctattaaat tgggactaag aactccgagc cgtatacgag ctgaaaaaaa tattcaaatc ctggctgatg aaattgtaga atatgcgcag atgataaacg cattatcaaa taattatttt gaaccaatgc aacgctcaac acaagcagtc gaaatgttga gtgacgatgt gctgcatctg cttaatctac caaatgacat acggcgacat actttagaac agcaggatac ttctaaagtt cagaaagaac tttcgtattt tttagatgag atggcttttc gtctttcaga aaacgagaat ggaatgctta gaagcaataa ttataaaatg cttgctaaga gcctggatat atcaaaacat aaagcacagg aaatcataaa tattgtacgg caagtggaaa gcctgcaact agagattcaa gagattaaca aaaacagatt ttctcaagaa aaacatgcag aggtcatcga aaacgatcag aatatttatg agtcatatga ccctcaaacg cagagttctg aaagtcttct taatccttca aaagaaataa atcctacaaa agtaatcgat tctatctatg acgntccttc aaaagaagta gatcctgtct atgcaaatta tatcccaaac aaaacttcaa acgcagantt ctagaagttt tcttantcct ccacaagaat taggtcctnt nactcagaat gaaattatta ataaaatttt tgataatatt attgttcaaa ggcatatagg agaaattaag catcnntcag gaattgtatt tgggaatana gaagtattta acagcgttct aacaaacatc attgagacaa aaaaaccaga agaaatcgga gaagggtata tcaaagtnnt agaagttgct cctaaaaact ttgctaaact tgctggtatt aaaatgtggg ggctaaaaaa tccgagatat aaacaagcta aagaaaatgt cccagaactc tgtaaagcaa ttaaagatta tacaaatgtt gtaaaaaaat ataaggaaca aattctttcc catcataaag aagaacaaaa acgtgtcaaa caagcggtat taatgcctaa taatgaaata cagcgttttt taaatatgcc aagtgatatg ttggaaaata ttttaataaa gcaaaatatt tctcaacttc ataaagaatt ttctaatttt ttaaatcaag ttcattctcg tcttacagca caagaacgta aagcgattaa gcgtaatgat tatcatacgc tcgctcgcaa tattaaggta ccagaagtta aagcacgtga gattataaat gtcgtaaaaa aagcacaaga atgccaacaa aaaattaaag atataatatc tcagaaacag

Expected Protein Sequence

MAHHHHHHMT LANKTEGFGH FLIPPKQLSI SVPDTSQDKN IDGYFSAKKR AVVKTLSAIV FRDGRALDRA IDRVTEHPGS VYALAKMITE KPETFGKLAG SIKLGLRTPS RIRAEKNIQI LADEIVEYAQ MINALSNNYF EPMQRSTQAV EMLSDDVLHL LNLPNDIRRH TLEQQDTSKV QKELSYFLDE MAFRLSENEN GMLRSNNYKM LAKSLDISKH KAQEIINIVR QVESLQLEIQ EINKNRFSQE KHAEVIENDQ NIYESYDPQT QSSESLLNPS KEINPTKVID SIYDNPSKEV DPVYANYTPN KTQTQNSRSF LNSAKKVDPV YDDPSKEINP TKVIDSIYDN PSKEVDPVYA NYTPNKTQTQ NSRSFLNSAK KIDPVYDDPS KEINPTKVID SIYDNPSKEV DPVYANYTPN KTQTQNSRSF LNSAKKVDPV YDDPSKEINP TKVIDSIYDN PSKEVDPVYA NYTPNKTQTQ NSRSFLNSAK KIDPVYDDPS KEIDSVYANY IPNKTQTQNS RSFLIPPQEL GPLTQNEIIN KIFDNIIVQR HIGEIKHLSG IVFGNKEVFN SVLTNIIETK KPEEIGEGYI KVLEVAPKNF AKLAGIKMWG LKNPRYKQAK ENVPELCKAI KDYTNVVKKY KEQILSHHKE EQKRVKQAVL MPNNEIQRFL NMPSDMLENI LIKQNISQLH KEFSNFLNQV HSRLTAQERK AIKRNDYHTL ARNIKVPEVK AREIINVVKK AQECQQKIKD IISQKQKQMI AVAH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac ttgcaaataa aacagaagga tttgggcatt ttttaattcc tccaaaacaa ttatctattt ctgtgcccga tacatcacag gacaaaaaca tagatggtta tttttctgca aagaagagag ctgtagttaa aactttgtca gcgattgtgt ttagagacgg gagggcttta gatagggcaa tagatcgggt tactgaacat ccaggttctg tgtatgcatt ggccaaaatg ataacagaaa aacctgaaac tttcggtaaa cttgcaggtt ctattaaatt gggactaaga actccgagcc gtatacgagc tgaaaaaaat attcaaatcc tggctgatga aattgtagaa tatgcgcaga tgataaacgc attatcaaat aattattttg aaccaatgca acgctcaaca caagcagtcg aaatgttgag tgacgatgtg ctgcatctgc ttaatctacc aaatgacata cggcgacata ctttagaaca gcaggatact tctaaagttc agaaagaact ttcgtatttt ttagatgaga tggcttttcg tctttcagaa aacgagaatg gaatgcttag aagcaataat tataaaatgc ttgctaagag cctggatata tcaaaacata aagcacagga aatcataaat attgtacggc aagtggaaag cctgcaacta gagattcaag agattaacaa aaacagattt tctcaagaaa aacatgcaga ggtcatcgaa aacgatcaga atatttatga gtcatatgac cctcaaacgc agagttctga aagtcttctt aatccttcaa aagaaataaa tcctacaaaa gtaatcgatt ctatctatga taatccttca aaagaagtag atcctgtcta tgcaaattat accccaaaca aaactcaaac gcagaattct agaagttttc ttaattctgc aaaaaaagta gatcctgtct atgacgatcc ttcaaaagaa ataaatccta caaaagtaat cgattctatc tatgataatc cttcaaaaga agtagatcct gtctatgcaa attatacccc aaacaaaact caaacgcaga attctagaag ttttcttaat tctgcaaaaa aaatagatcc tgtctatgac gatccttcaa aagaaataaa tcctacaaaa gtaatcgatt ctatctatga taatccttca aaagaagtag atcctgtcta tgcaaattat accccaaaca aaactcaaac gcagaattct agaagttttc ttaattctgc aaaaaaagta gatcctgtct atgacgatcc ttcaaaagaa ataaatccta caaaagtaat cgattctatc tatgataatc cttcaaaaga agtagatcct gtctatgcaa attatacccc aaacaaaact caaacgcaga attctagaag ttttcttaat tctgcaaaaa aaatagatcc tgtctatgac gatccttcaa aagaaataga ttctgtctat gcaaattata tcccaaacaa aactcaaacg cagaattcta gaagttttct tattcctcca caagaattag gtcctctaac tcagaatgaa attattaata aaatttttga taatattatt gttcaaaggc atataggaga aattaagcat ctttcaggaa ttgtatttgg gaataaagaa gtatttaaca gcgttctaac aaacatcatt gagacaaaaa aaccagaaga aatcggagaa gggtatatca aagtactaga agttgctcct aaaaactttg ctaaacttgc tggtattaaa atgtgggggc taaaaaatcc gagatataaa caagctaaag aaaatgtccc agaactctgt aaagcaatta aagattatac aaatgttgta aaaaaatata aggaacaaat tctttcccat cataaagaag aacaaaaacg tgtcaaacaa gcggtattaa tgcctaataa tgaaatacag cgttttttaa atatgccaag tgatatgttg gaaaatattt taataaagca aaatatttct caacttcata aagaattttc taatttttta aatcaagttc attctcgtct tacagcacaa gaacgtaaag cgattaagcg taatgattat catacgctcg ctcgcaatat taaggtacca gaagttaaag cacgtgagat tataaatgtc gtaaaaaaag cacaagaatg ccaacaaaaa attaaagata taatatctca gaaacagaaa caaatgatag ctgtagctca ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BasaA.18410.a.B2.GE37360

HARVESTED ON:	9/27/2013
SEQUENCED ON:	10/3/2013
EXPECTED MW:	16kDa
OBSERVED MW:	16kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML IPPKQLSISV PDTSQDKNID GYFSAKKRAV VKTLSAIVFR DGRALDRAID RVTEHPGSVY ALAKMITEKP ETFGKLAGSI KLGLRTPSRI RAEKNIQILA DEIVEYAQMI NALSNNYFEP MQRSTQAVEM LSD

Validated NT Sequence

atcttactca cttagtcact caacatttcg actgcttgtg ttgagcgttg cattggttca aaataattat ttgataatgc gtttatcatc tgcgcatatt ctacaatttc atcagccagg atttgaatat ttttttcagc tcgtatacgg ctcggagttc ttagtcccaa tttaatagaa cctgcaagtt taccgaaagt ttcaggtttt tctgttatca ttttggccaa tgcatacaca gaacctggat gttcagtaac ccgatctatt gccctatcta aagccctccc gtctctaaac acaatcgctg acaaagtttt aactacagct ctcttctttg cagaaaaata accatctatg tttttgtcct gtgatgtatc gggcacagaa atagataatt gttttggagg aattaacata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHLI PPKQLSISVP DTSQDKNIDG YFSAKKRAVV KTLSAIVFRD GRALDRAIDR VTEHPGSVYA LAKMITEKPE TFGKLAGSIK LGLRTPSRIR AEKNIQILAD EIVEYAQMIN ALSNNYFEPM QRSTQAVEML SD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttaattc ctccaaaaca attatctatt tctgtgcccg atacatcaca ggacaaaaac atagatggtt atttttctgc aaagaagaga gctgtagtta aaactttgtc agcgattgtg tttagagacg ggagggcttt agatagggca atagatcggg ttactgaaca tccaggttct gtgtatgcat tggccaaaat gataacagaa aaacctgaaa ctttcggtaa acttgcaggt tctattaaat tgggactaag aactccgagc cgtatacgag ctgaaaaaaa tattcaaatc ctggctgatg aaattgtaga atatgcgcag atgataaacg cattatcaaa taattatttt gaaccaatgc aacgctcaac acaagcagtc gaaatgttga gtgactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat