SSGCID: Target Page

BasaA.18404.b

Effector protein yopJ (Yop-like family protein)

CENTER ID:	BasaA.18404.b
ORGANISM:	Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BasaA.18404.b.B1.GE35666	full length		1	280

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	E6YPL0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRKLLNTIKN AVGLSQTEDL ENIITNLEND IANGDWLNTT YTEGDFQIMP ALIKQANNKY PEMHLTLIKE PRDFVASILK KIKNGIKSSR YIINVGGNDD LVHFAVVDHQ TIDNKTSLIL FEPARFSLPI PAILGAKMQT VIETRLPHCY FSLAEMDIQR SMSECGIFCL FLAKKFYRHS DKLEKLHRDN IKGDQWDRIA HLSHDLLDTY LPINFYKHTQ GVRRLQEYID KNPGSEDKII NKKNETISDR VNKNIVVLKG KCISISAHKK RVTEYKSLMR

NT Sequence

atgcgaaaat tattgaatac aataaaaaat gctgttggtt tatctcagac agaagatcta gaaaatatta tcacaaattt agagaatgac attgctaatg gtgattggct taacactact tacacagaag gagattttca aataatgcct gccttgataa agcaggcgaa taataaatat ccagagatgc accttacatt gattaaagag cctagagatt tcgtcgcatc aattttaaaa aaaattaaaa acggaattaa atcttctagg tatataatta acgtaggagg aaatgatgat ctcgttcatt ttgcagtagt agatcaccaa actatcgata acaaaacatc cctgatatta tttgaacctg caagattctc tcttccaatt ccagcgattc taggagcaaa aatgcaaacg gtcatagaaa ctcggttacc tcactgttat ttttctttag cggaaatgga tattcagcga agtatgtctg aatgtggaat tttttgttta tttctcgcta aaaaatttta tcgccattct gataaattag aaaaattaca tagagataat attaaaggtg atcagtggga tagaatagcc catttatctc atgatttgtt ggatacatat cttccaatca acttttataa acacacgcaa ggcgtgagac gccttcagga atatatagat aaaaatccag gatctgaaga caaaatcatt aacaaaaaaa atgaaactat ttctgataga gtaaacaaaa atatagttgt actaaagggt aaatgtatat ctatctcagc acacaaaaaa agagttactg aatataaatc tctgatgaga taa

Details for BasaA.18404.b.B1.GE35666

HARVESTED ON:	7/10/2012
SEQUENCED ON:	7/30/2012
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR KLLNTIKNAV GLSQTEDLEN IITNLENDIA NGDWLNTTYT EGDFQIMPAL IKQANNKYPE MHLTLIKEPR DFVASILKKI KNGIKSSRYI INVGGNDDLV HFAVVDHQTI DNKTSLILFE PARFSLPIPA ILGAKMQTVI ETRLPHCYFS LAEMDIQRSM SECGIFCLFL AKKFYRHSDK LEKLHRDNIK GDQWDRIAHL SHDLLDTYLP INFYKHTQGV RRLQEYIDKN PGSEDKIINK KNETISDRVN KNIVVLKGKC ISISAHKKRV TEYKSLXR

Validated NT Sequence

ttttgtnnnn tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgaa aattattgaa tacaataaaa aatgctgttg gtttatctca gacagaagat ctagaaaata ttatcacaaa tttagagaat gacattgcta atggtgattg gcttaacact acttacacag aaggagattt tcaaataatg cctgccttga taaagcaggc gaataataaa tatccagaga tgcaccttac attgattaaa gagcctagag atttcgtcgc atcaatttta aaaaaaatta aaaacggaat taaatcttct aggtatataa ttaacgtagg aggaaatgat gatctcgttc attttgcagt agtagatcac caaactatcg ataacaaaac atccctgata ttatttgaac ctgcaagatt ctctcttcca attccagcga ttctaggagc aaaaatgcaa acggtcatag aaactcggtt acctcactgt tatttttctt tagcggaaat ggatattcag cgaagtatgt ctgaatgtgg aattttttgt ttatttctcg ctaaaaaatt ttatcgccat tctgataaat tagaaaaatt acatagagat aatattaaag gtgatcagtg ggatagaata gcccatttat ctcatgattt gttggataca tatcttccaa tcaactttta taaacacacg caaggcgtga gacgccttca ggaatatata gataaaaatc caggatctga agacaaaatc attaacaaaa aaaatgaaac tatttctgat agagtaaaca aaaatatagt tgtactaaag ggtaaatgta tatctatctc agcacacaaa aaaagagtta ctgaatataa atctctgang agataagtga gtaananagg ancnnnnnnn nnnccccccn nnnnnnnnnn nnnnnnnnna annaaannna aaannnnnna nna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgaa aattattgaa tacaataaaa aatgctgttg gtttatctca gacagaagat ctagaaaata ttatcacaaa tttagagaat gacattgcta atggtgattg gcttaacact acttacacag aaggagattt tcaaataatg cctgccttga taaagcaggc gaataataaa tatccagaga tgcaccttac attgattaaa gagcctagag atttcgtcgc atcaatttta aaaaaaatta aaaacggaat taaatcttct aggtatataa ttaacgtagg aggaaatgat gatctcgttc attttgcagt agtagatcac caaactatcg ataacaaaac atccctgata ttatttgaac ctgcaagatt ctctcttcca attccagcga ttctaggagc aaaaatgcaa acggtcatag aaactcggtt acctcactgt tatttttctt tagcggaaat ggatattcag cgaagtatgt ctgaatgtgg aattttttgt ttatttctcg ctaaaaaatt ttatcgccat tctgataaat tagaaaaatt acatagagat aatattaaag gtgatcagtg ggatagaata gcccatttat ctcatgattt gttggataca tatcttccaa tcaactttta taaacacacg caaggcgtga gacgccttca ggaatatata gataaaaatc caggatctga agacaaaatc attaacaaaa aaaatgaaac tatttctgat agagtaaaca aaaatatagt tgtactaaag ggtaaatgta tatctatctc agcacacaaa aaaagagtta ctgaatataa atctctgatg agatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat