SSGCID: Target Page

BasaA.17987.a

DNA polymerase III subunit beta (EC 2.7.7.7)

CENTER ID:	BasaA.17987.a
ORGANISM:	Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BasaA.17987.a.B1.GE41239	full length		1	373

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	E6YP51

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRVTISRSQL LKSLGRVHRI VERRNTIPIL SNVLIDASDG RMQLKATDLD LEITESSSVN IEQSGAITVP AHLLYDIVRK LLDDSEVILD VDESQASTMS ITSGCTNFQL QCLPKVDFPE SLPGQFSCRF SLLASKLKYL IDCTQFAMST EETRYYLNGI YFHIISDDIS KLRLVSTDGH RLAQVDIEAP LGSDGMPGVI IPRKTVGELQ KLLGEEADND VCIELSDTKI RFSVGSVILT SKLIDGVFPE YQRVIPLKND KKLIINRRDF SSAVDRVSTI SSDRGRAVKL MIEKGLVRFV VNNPDSGSAK DQLVADYAFD PLEIGFNSRY LLDIAAQLSS DEMIFTFADS GAPTLIRGNS DTDSLYVLMP IRV

NT Sequence

atgcgcgtta caattagtcg tagtcagcta cttaaatctc ttggtcgtgt tcatcgtatt gtggagcgtc gtaatactat ccctattttg tcaaatgtat tgatagatgc gagtgatgga aggatgcaat taaaagcaac agatctcgat ctagaaatta cagaatcttc ttcagtcaat attgaacaaa gcggggcgat aactgttcca gcgcatttgc tctacgatat tgttcgcaaa cttcttgacg atagtgaggt tattttagac gttgatgaaa gtcaggcaag tactatgtct attacctctg gttgtactaa ttttcagctt caatgtcttc ctaaggtaga ttttccagaa tcgcttccag gccagtttag ttgtcgtttt tctttgttag cgtcaaaatt gaaatattta attgattgta cgcagtttgc tatgtctact gaagagacac gttattatct gaatggtatt tattttcata tcatttctga tgatatttcg aagttgcgtt tagtttcgac tgatggtcat cgtttagcac aggtcgatat agaagcacct ttggggtctg atggtatgcc aggtgttatc attcctcgta aaaccgtggg agagttgcaa aaacttcttg gggaagaagc tgataatgat gtgtgtatag agctttcaga tacaaagatt cgtttttctg ttgggtctgt aattttaaca tcaaaactaa ttgatggggt atttccagaa tatcaacgtg ttattccatt aaaaaatgat aaaaaattga ttatcaatcg acgagatttt tcttctgcgg ttgatcgtgt ctcaacaatt tcaagcgatc gtgggcgtgc agtaaaattg atgatcgaga aaggacttgt aagatttgta gtgaataatc ctgattcagg aagtgcgaag gatcagttgg ttgcagatta tgcatttgat ccgcttgaga taggtttcaa ttcgcgatat cttttggata ttgcagcaca actttcaagt gatgaaatga tttttacgtt tgctgattct ggagcgccaa cgttgattcg tggaaatagt gatacagatt cgctttatgt gctgatgcct attcgtgttt aa

Details for BasaA.17987.a.B1.GE41239

HARVESTED ON:	data unavailable
SEQUENCED ON:	3/9/2017
EXPECTED MW:	42kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMP VTISRSQLLK SLGRVHRIVE RRNTIPILSN VLIDASDGRM QLKATDLDLE ITESSSVNIE QSGAITVPAH LLYDIVRKLL DDSEVILDVD ESQASTMSIT SGCTNFQLQC LPKVDFPESL PGQFSCRFSL LASKLKYLID CTQFAMSTEE TRYYLNGIYF HIISDDISKL RLVSTDGHRL AQVDIEAPLG SDGMPGVIIP RKTVGELQKL LGEEADNDVC IELSDTKIRF SVGSVILTSK LIDGVFPEYQ RVIPLKNDKK LIINRRDFSS AVDRVSTISS DRGRAVKLMI EKGLVRFVVN NPDSGSAKDQ LVADYAFDPL EIGFNSRYLL DIAAQLSSDE MIFTFADSGA PTLIRGNSDT DS

Validated NT Sequence

atggctcacc accaccacca ccatatgccc gttacaatta gtcgtagtca gctacttaaa tctcttggtc gtgttcatcg tattgtggag cgtcgtaata ctatccctat tttgtcaaat gtattgatag atgcgagtga tggaaggatg caattaaaag caacagatct cgatctagaa attacagaat cttcttcagt caatattgaa caaagcgggg cgataactgt tccagcgcat ttgctctacg atattgttcg caaacttctt gacgatagtg aggttatttt agacgttgat gaaagtcagg caagtactat gtctattacc tctggttgta ctaattttca gcttcaatgt cttcctaagg tagattttcc agaatcgctt ccaggccagt ttagttgtcg tttttctttg ttagcgtcaa aattgaaata tttaattgat tgtacgcagt ttgctatgtc tactgaagag acacgttatt atctgaatgg tatttatttt catatcattt ctgatgatat ttcgaagttg cgtttagttt cgactgatgg tcatcgttta gcacaggtcg atatagaagc acctttgggg tctgatggta tgccaggtgt tatcattcct cgtaaaaccg tgggagagtt gcaaaaactt cttggggaag aagctgataa tgatgtgtgt atagagcttt cagatacaaa gattcgtttt tctgttgggt ctgtaatttt aacatcaaaa ctaattgatg gggtatttcc agaatatcaa cgtgttattc cattaaaaaa tgataaaaaa ttgattatca atcgacgaga tttttcttct gcggttgatc gtgtctcaac aatttcaagc gatcgtgggc gtgcagtaaa attgatgatc gagaaaggac ttgtaagatt tgtagtgaat aatcctgatt caggaagtgc gaaggatcag ttggttgcag attatgcatt tgatccgctt gagataggtt tcaattcgcg atatcttttg gatattgcag cacaactttc aagtgatgaa atgattttta cgtttgctga ttctggagcg ccaacgttga ttcgtggaaa tagtgataca gattcgct

Expected Protein Sequence

MAHHHHHHMR VTISRSQLLK SLGRVHRIVE RRNTIPILSN VLIDASDGRM QLKATDLDLE ITESSSVNIE QSGAITVPAH LLYDIVRKLL DDSEVILDVD ESQASTMSIT SGCTNFQLQC LPKVDFPESL PGQFSCRFSL LASKLKYLID CTQFAMSTEE TRYYLNGIYF HIISDDISKL RLVSTDGHRL AQVDIEAPLG SDGMPGVIIP RKTVGELQKL LGEEADNDVC IELSDTKIRF SVGSVILTSK LIDGVFPEYQ RVIPLKNDKK LIINRRDFSS AVDRVSTISS DRGRAVKLMI EKGLVRFVVN NPDSGSAKDQ LVADYAFDPL EIGFNSRYLL DIAAQLSSDE MIFTFADSGA PTLIRGNSDT DSLYVLMPIR V

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcg ttacaattag tcgtagtcag ctacttaaat ctcttggtcg tgttcatcgt attgtggagc gtcgtaatac tatccctatt ttgtcaaatg tattgataga tgcgagtgat ggaaggatgc aattaaaagc aacagatctc gatctagaaa ttacagaatc ttcttcagtc aatattgaac aaagcggggc gataactgtt ccagcgcatt tgctctacga tattgttcgc aaacttcttg acgatagtga ggttatttta gacgttgatg aaagtcaggc aagtactatg tctattacct ctggttgtac taattttcag cttcaatgtc ttcctaaggt agattttcca gaatcgcttc caggccagtt tagttgtcgt ttttctttgt tagcgtcaaa attgaaatat ttaattgatt gtacgcagtt tgctatgtct actgaagaga cacgttatta tctgaatggt atttattttc atatcatttc tgatgatatt tcgaagttgc gtttagtttc gactgatggt catcgtttag cacaggtcga tatagaagca cctttggggt ctgatggtat gccaggtgtt atcattcctc gtaaaaccgt gggagagttg caaaaacttc ttggggaaga agctgataat gatgtgtgta tagagctttc agatacaaag attcgttttt ctgttgggtc tgtaatttta acatcaaaac taattgatgg ggtatttcca gaatatcaac gtgttattcc attaaaaaat gataaaaaat tgattatcaa tcgacgagat ttttcttctg cggttgatcg tgtctcaaca atttcaagcg atcgtgggcg tgcagtaaaa ttgatgatcg agaaaggact tgtaagattt gtagtgaata atcctgattc aggaagtgcg aaggatcagt tggttgcaga ttatgcattt gatccgcttg agataggttt caattcgcga tatcttttgg atattgcagc acaactttca agtgatgaaa tgatttttac gtttgctgat tctggagcgc caacgttgat tcgtggaaat agtgatacag attcgcttta tgtgctgatg cctattcgtg tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat