BasaA.17330.k

Bep1 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BasaA.17330.k
ORGANISM: Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BasaA.17330.k.B1.GE35711 full length 1 557
BasaA.17330.k.B2.GE37357 FIC 21 253
BasaA.17330.k.B3.GE37358 FIC and OB-Fold 21 309
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: E6YNY8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKQTLSNEAI MTESASSLPS PNNYNYPNSL TLKNKYGIIN HQEFMDKCAH DSAKAAINLR QEALPKKFDS SYLKYLHKRL FESSFEWAGC TRDIPFPFKD GTLAVMPEMM RSNWKTDKPI VFATGNKVQD GLKNIDRMLI EKDNLQNLPR QEFIHHIAEI FSSLNYTHPF REGNGRTQRL FCEKLAQAAN YNLDFSVVTK ERMSEVSIAA AQDGNLEPMK KLFDDISDPH KTAILKEFIS NIQNIDQEDI NDCIIVAADK GVTYTGIYKG ASPNSIIIKT ENTYTICSKD HLTPEQLKTL NLNNECTLKI PTTQNKENVL IPKEILAPLT SKQILKKIQN NTDIQNKKKE IKHLSKLVYA SAKTLNQQIK VINNNQRLSK QLSSQIISSP NSVANLAGFE ILGMKSPARK KAEDHAIKLA NSIDTYASII KNVKATIIQQ YKSEQKRLQT TVKLPSPTLQ NILNLSEEKR KELLSEEKLS SSIARELDEF MHALNTRLSP SERKNIHESD HQQLAKSVGI SENKAATIIK VAQEAKEVSQ QIQKLAFSVE KQFIVAN
NT Sequence
atgaaacaaa cactctcaaa cgaagcaata atgacagaat cagcatctag tttgccttca cctaataatt ataactatcc caatagtctt acattaaaga ataaatatgg aatcataaat catcaagaat ttatggataa atgtgcacat gattcagcaa aagcagccat taatttgcgt caagaagcac tgccaaagaa gtttgactct tcctatttaa aatatcttca taaacgctta tttgaaagct cgtttgaatg ggctggttgt actcgtgata tcccttttcc atttaaagat ggtacacttg ctgttatgcc agaaatgatg agatcaaatt ggaaaacaga caaacctatt gtttttgcaa ccggtaataa agtgcaagat ggtttgaaaa atatagatag aatgctcatc gaaaaggata atttacaaaa cttaccacgc caagagttta ttcatcatat agccgaaata ttttcttctc tcaactacac gcatcctttt agagaaggca atggacgtac acaacggcta ttttgcgaaa aacttgctca agctgcaaat tataatcttg acttttcagt tgtaacaaaa gagcgtatgt ctgaggtcag tattgcagca gcacaagatg gcaatctaga accgatgaaa aagctttttg atgatatctc cgaccctcac aaaacagcta ttttaaagga attcataagc aatatacaaa atattgatca agaagatatt aatgattgta ttattgtagc agcggataaa ggtgtaacct atacaggaat ttataaaggt gcaagcccaa atagtattat aatcaaaaca gaaaatactt atacgatctg tagcaaagat catctcacac cagaacaatt aaaaacatta aatcttaata atgaatgtac cctgaaaatt ccaaccactc aaaataaaga aaatgtttta attccaaaag aaatattagc tcctttgaca tcaaaacaaa tattaaaaaa aattcaaaac aatactgaca ttcaaaacaa aaaaaaagaa atcaaacatt tatcaaaact tgtatatgct agcgctaaaa cattaaatca acagataaag gtaattaata ataatcaaag gctaagcaaa cagctttcct cacaaattat atcttctccc aactctgttg ctaatcttgc aggctttgaa atattgggta tgaaaagccc agcgcgtaaa aaagctgaag atcatgctat caaactcgct aactcgattg acacttatgc atctattata aaaaatgtta aggcaacaat aatccaacag tacaaatcag aacaaaaacg ccttcaaaca acggtaaaac tacccagccc aacattgcaa aatattctta acttatcaga agaaaagcga aaggaattgt tatcagagga aaagttatct tcttctattg ccagagaact ggatgaattt atgcatgcac tcaatactcg tctttcacca agcgagcgta aaaacattca tgaaagtgat catcaacaat tagctaaaag tgttggcata tcagaaaata aagcagcaac aatcatcaaa gttgctcaag aagctaaaga ggtatcccaa caaatacaaa aactagcctt tagtgttgaa aaacagttta ttgtagcaaa ctaa
Details for BasaA.17330.k.B1.GE35711
HARVESTED ON: 7/10/2012
SEQUENCED ON: 2/22/2016
EXPECTED MW: 63kDa
OBSERVED MW: 63kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 56
Validated AA Sequence
MAHHHHHHMK QTLSNEAIMT ESASSLPSPN NYNYPNSLTL KNKYGIINHQ EFMDKCAHDS AKAAINLRQE ALPKKFDSSY LKYLHKRLFE SSFEWAGCTR DIPFPFKDGT LAVMPEMMRS NWKTDKPIVF ATGNKVQDGL KNIDRMLIEK DNLQNLPRQE FIHHIAEIFS SLNYTHPFRE GNGRTQRLFC EKLAQAANYN LDFSVVTKER MSEVSIAAAQ DGNLEPMKKL FDDISDPHKT AILKEFISNI QNIDQEDIND CIIVAADKGV TYTGIYKGAS PNSIIIKTEN TYTICSKDHL TPXXLKXLNL XXXCTXX
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa caaacactct caaacgaagc aataatgaca gaatcagcat ctagtttgcc ttcacctaat aattataact atcccaatag tcttacatta aagaataaat atggaatcat aaatcatcaa gaatttatgg ataaatgtgc acatgattca gcaaaagcag ccattaattt gcgtcaagaa gcactgccaa agaagtttga ctcttcctat ttaaaatatc ttcataaacg cttatttgaa agctcgtttg aatgggctgg ttgtactcgt gatatccctt ttccatttaa agatggtaca cttgctgtta tgccagaaat gatgagatca aattggaaaa cagacaaacc tattgttttt gcaaccggta ataaagtgca agatggtttg aaaaatatag atagaatgct catcgaaaag gataatttac aaaacttacc acgccaagag tttattcatc atatagccga aatattttct tctctcaact acacgcatcc ttttagagaa ggcaatggac gtacacaacg gctattttgc gaaaaacttg ctcaagctgc aaattataat cttgactttt cagttgtaac aaaagagcgt atgtctgagg tcagtattgc agcagcacaa gatggcaatc tagaaccgat gaaaaagctt tttgatgata tctccgaccc tcacaaaaca gctattttaa aggaattcat aagcaatata caaaatattg atcaagaaga tattaatgat tgtattattg tagcagcgga taaaggtgta acctatacag gaatttataa aggtgcaagc ccaaatagta ttataatcaa aacagaaaat acttatacga tctgtagcaa agatcatctc acaccnnnnn aattaaaanc attaaatctn nnnaannaat gtacccnnna aaat
Expected Protein Sequence
MAHHHHHHMK QTLSNEAIMT ESASSLPSPN NYNYPNSLTL KNKYGIINHQ EFMDKCAHDS AKAAINLRQE ALPKKFDSSY LKYLHKRLFE SSFEWAGCTR DIPFPFKDGT LAVMPEMMRS NWKTDKPIVF ATGNKVQDGL KNIDRMLIEK DNLQNLPRQE FIHHIAEIFS SLNYTHPFRE GNGRTQRLFC EKLAQAANYN LDFSVVTKER MSEVSIAAAQ DGNLEPMKKL FDDISDPHKT AILKEFISNI QNIDQEDIND CIIVAADKGV TYTGIYKGAS PNSIIIKTEN TYTICSKDHL TPEQLKTLNL NNECTLKIPT TQNKENVLIP KEILAPLTSK QILKKIQNNT DIQNKKKEIK HLSKLVYASA KTLNQQIKVI NNNQRLSKQL SSQIISSPNS VANLAGFEIL GMKSPARKKA EDHAIKLANS IDTYASIIKN VKATIIQQYK SEQKRLQTTV KLPSPTLQNI LNLSEEKRKE LLSEEKLSSS IARELDEFMH ALNTRLSPSE RKNIHESDHQ QLAKSVGISE NKAATIIKVA QEAKEVSQQI QKLAFSVEKQ FIVAN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac aaacactctc aaacgaagca ataatgacag aatcagcatc tagtttgcct tcacctaata attataacta tcccaatagt cttacattaa agaataaata tggaatcata aatcatcaag aatttatgga taaatgtgca catgattcag caaaagcagc cattaatttg cgtcaagaag cactgccaaa gaagtttgac tcttcctatt taaaatatct tcataaacgc ttatttgaaa gctcgtttga atgggctggt tgtactcgtg atatcccttt tccatttaaa gatggtacac ttgctgttat gccagaaatg atgagatcaa attggaaaac agacaaacct attgtttttg caaccggtaa taaagtgcaa gatggtttga aaaatataga tagaatgctc atcgaaaagg ataatttaca aaacttacca cgccaagagt ttattcatca tatagccgaa atattttctt ctctcaacta cacgcatcct tttagagaag gcaatggacg tacacaacgg ctattttgcg aaaaacttgc tcaagctgca aattataatc ttgacttttc agttgtaaca aaagagcgta tgtctgaggt cagtattgca gcagcacaag atggcaatct agaaccgatg aaaaagcttt ttgatgatat ctccgaccct cacaaaacag ctattttaaa ggaattcata agcaatatac aaaatattga tcaagaagat attaatgatt gtattattgt agcagcggat aaaggtgtaa cctatacagg aatttataaa ggtgcaagcc caaatagtat tataatcaaa acagaaaata cttatacgat ctgtagcaaa gatcatctca caccagaaca attaaaaaca ttaaatctta ataatgaatg taccctgaaa attccaacca ctcaaaataa agaaaatgtt ttaattccaa aagaaatatt agctcctttg acatcaaaac aaatattaaa aaaaattcaa aacaatactg acattcaaaa caaaaaaaaa gaaatcaaac atttatcaaa acttgtatat gctagcgcta aaacattaaa tcaacagata aaggtaatta ataataatca aaggctaagc aaacagcttt cctcacaaat tatatcttct cccaactctg ttgctaatct tgcaggcttt gaaatattgg gtatgaaaag cccagcgcgt aaaaaagctg aagatcatgc tatcaaactc gctaactcga ttgacactta tgcatctatt ataaaaaatg ttaaggcaac aataatccaa cagtacaaat cagaacaaaa acgccttcaa acaacggtaa aactacccag cccaacattg caaaatattc ttaacttatc agaagaaaag cgaaaggaat tgttatcaga ggaaaagtta tcttcttcta ttgccagaga actggatgaa tttatgcatg cactcaatac tcgtctttca ccaagcgagc gtaaaaacat tcatgaaagt gatcatcaac aattagctaa aagtgttggc atatcagaaa ataaagcagc aacaatcatc aaagttgctc aagaagctaa agaggtatcc caacaaatac aaaaactagc ctttagtgtt gaaaaacagt ttattgtagc aaactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BasaA.17330.k.B2.GE37357
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP NNYNYPNSLT LKNKYGIINH QEFMDKCAHD SAKAAINLRQ EALPKKFDSS YLKYLHKRLF ESSFEWAGCT RDIPFPFKDG TLAVMPEMMR SNWKTDKPIV FATGNKVQDG LKNIDRMLIE KDNLQNLPRQ EFIHHIAEIF SSLNYTHPFR EGNGRTQRLF CEKLAQAANY NLDFSVVTKE RMSEVSIAAA QDGNLEPMKK LFDDISDPHK TAILKEFISN IQNIDQEDIN DC
Validated NT Sequence
atcttactca cttaacaatc attaatatct tcttgatcaa tattttgtat attgcttatg aattccttta aaatagctgt tttgtgaggg tcggagatat catcaaaaag ctttttcatc ggttctagat tgccatcttg tgctgctgca atactgacct cagacatacg ctcttttgtt acaactgaaa agtcaagatt ataatttgca gcttgagcaa gtttttcgca aaatagccgt tgtgtacgtc cattgccttc tctaaaagga tgcgtgtagt tgagagaaga aaatatttcg gctatatgat gaataaactc ttggcgtggt aagttttgta aattatcctt ttcgatgagc attctatcta tatttttcaa accatcttgc actttattac cggttgcaaa aacaataggt ttgtctgttt tccaatttga tctcatcatt tctggcataa cagcaagtgt accatcttta aatggaaaag ggatatcacg agtacaacca gcccattcaa acgagctttc aaataagcgt ttatgaagat attttaaata ggaagagtca aacttctttg gcagtgcttc ttgacgcaaa ttaatggctg cttttgctga atcatgtgca catttatcca taaattcttg atgatttatg attccatatt tattctttaa tgtaagacta ttgggatagt tataattatt aggcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHPN NYNYPNSLTL KNKYGIINHQ EFMDKCAHDS AKAAINLRQE ALPKKFDSSY LKYLHKRLFE SSFEWAGCTR DIPFPFKDGT LAVMPEMMRS NWKTDKPIVF ATGNKVQDGL KNIDRMLIEK DNLQNLPRQE FIHHIAEIFS SLNYTHPFRE GNGRTQRLFC EKLAQAANYN LDFSVVTKER MSEVSIAAAQ DGNLEPMKKL FDDISDPHKT AILKEFISNI QNIDQEDIND C
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcctaata attataacta tcccaatagt cttacattaa agaataaata tggaatcata aatcatcaag aatttatgga taaatgtgca catgattcag caaaagcagc cattaatttg cgtcaagaag cactgccaaa gaagtttgac tcttcctatt taaaatatct tcataaacgc ttatttgaaa gctcgtttga atgggctggt tgtactcgtg atatcccttt tccatttaaa gatggtacac ttgctgttat gccagaaatg atgagatcaa attggaaaac agacaaacct attgtttttg caaccggtaa taaagtgcaa gatggtttga aaaatataga tagaatgctc atcgaaaagg ataatttaca aaacttacca cgccaagagt ttattcatca tatagccgaa atattttctt ctctcaacta cacgcatcct tttagagaag gcaatggacg tacacaacgg ctattttgcg aaaaacttgc tcaagctgca aattataatc ttgacttttc agttgtaaca aaagagcgta tgtctgaggt cagtattgca gcagcacaag atggcaatct agaaccgatg aaaaagcttt ttgatgatat ctccgaccct cacaaaacag ctattttaaa ggaattcata agcaatatac aaaatattga tcaagaagat attaatgatt gttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BasaA.17330.k.B3.GE37358
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP NNYNYPNSLT LKNKYGIINH QEFMDKCAHD SAKAAINLRQ EALPKKFDSS YLKYLHKRLF ESSFEWAGCT RDIPFPFKDG TLAVMPEMMR SNWKTDKPIV FATGNKVQDG LKNIDRMLIE KDNLQNLPRQ EFIHHIAEIF SSLNYTHPFR EGNGRTQRLF CEKLAQAANY NLDFSVVTKE RMSEVSIAAA QDGNLEPMKK LFDDISDPHK TAILKEFISN IQNIDQEDIN DCIIVAADKG VTYTGIYKGA SPNSIIIKTE NTYTICSKDH LTPEQLKTLN LNNECTLK
Validated NT Sequence
ttttgtttaa ctttaagaag gaganatacc atggctcacc accaccacca ccatatgcct aataattata actatcccaa tagtcttaca ttaaagaata aatatggaat cataaatcat caagaattta tggataaatg tgcacatgat tcagcaaaag cagccattaa tttgcgtcaa gaagcactgc caaagaagtt tgactcttcc tatttaaaat atcttcataa acgcttattt gaaagctcgt ttgaatgggc tggttgtact cgtgatatcc cttttccatt taaagatggt acacttgctg ttatgccaga aatgatgaga tcaaattgga aaacagacaa acctattgtt tttgcaaccg gtaataaagt gcaagatggt ttgaaaaata tagatagaat gctcatcgaa aaggataatt tacaaaactt accacgccaa gagtttattc atcatatagc cgaaatattt tcttctctca actacacgca tccttttaga gaaggcaatg gacgtacaca acggctattt tgcgaaaaac ttgctcaagc tgcaaattat aatcttgact tttcagttgt aacaaaagag cgtatgtctg aggtcagtat tgcagcagca caagatggca atctagaacc gatgaaaaag ctttttgatg atatctccga ccctcacaaa acagctattt taaaggaatt cataagcaat atacaaaata ttgatcaaga agatattaat gattgtatta ttgtagcagc ggataaaggt gtaacctata caggaattta taaaggtgca agcccaaata gtattataat caaaacagaa aatacttata cgatctgtag caaagatcat ctcacaccag aacaattaaa aacattaaat cttaataatg aatgtaccct gaaataagtg agtaaganag ganccnnnnn nnnnnnnncc
Expected Protein Sequence
MAHHHHHHPN NYNYPNSLTL KNKYGIINHQ EFMDKCAHDS AKAAINLRQE ALPKKFDSSY LKYLHKRLFE SSFEWAGCTR DIPFPFKDGT LAVMPEMMRS NWKTDKPIVF ATGNKVQDGL KNIDRMLIEK DNLQNLPRQE FIHHIAEIFS SLNYTHPFRE GNGRTQRLFC EKLAQAANYN LDFSVVTKER MSEVSIAAAQ DGNLEPMKKL FDDISDPHKT AILKEFISNI QNIDQEDIND CIIVAADKGV TYTGIYKGAS PNSIIIKTEN TYTICSKDHL TPEQLKTLNL NNECTLK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcctaata attataacta tcccaatagt cttacattaa agaataaata tggaatcata aatcatcaag aatttatgga taaatgtgca catgattcag caaaagcagc cattaatttg cgtcaagaag cactgccaaa gaagtttgac tcttcctatt taaaatatct tcataaacgc ttatttgaaa gctcgtttga atgggctggt tgtactcgtg atatcccttt tccatttaaa gatggtacac ttgctgttat gccagaaatg atgagatcaa attggaaaac agacaaacct attgtttttg caaccggtaa taaagtgcaa gatggtttga aaaatataga tagaatgctc atcgaaaagg ataatttaca aaacttacca cgccaagagt ttattcatca tatagccgaa atattttctt ctctcaacta cacgcatcct tttagagaag gcaatggacg tacacaacgg ctattttgcg aaaaacttgc tcaagctgca aattataatc ttgacttttc agttgtaaca aaagagcgta tgtctgaggt cagtattgca gcagcacaag atggcaatct agaaccgatg aaaaagcttt ttgatgatat ctccgaccct cacaaaacag ctattttaaa ggaattcata agcaatatac aaaatattga tcaagaagat attaatgatt gtattattgt agcagcggat aaaggtgtaa cctatacagg aatttataaa ggtgcaagcc caaatagtat tataatcaaa acagaaaata cttatacgat ctgtagcaaa gatcatctca caccagaaca attaaaaaca ttaaatctta ataatgaatg taccctgaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat