BasaA.17330.j

Bep5 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BasaA.17330.j
ORGANISM: Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BasaA.17330.j.B1.GE35710 full length 1 559
BasaA.17330.j.B3.GE37355 FIC and OB-Fold 31 313
BasaA.17330.j.B4.GE37356 BID 325 460
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BasaA.17330.j.B4.PS01938 BID 325 460

External Resources

RESOURCE REFERENCE ID
UniProt: E6YPK5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGYACRHYT LAQFNALMDA DREKVMLSNQ KKGWNYNSSA KIRNKYGLQG IDFEKRCVHD VIQASVNLKE EPLPEKFDSS YLKYLHKRLF ENTFEWAGYT REFPFTFSDG AVREDLTITI PDLDYSIGND KITNGLKEID RILAEKNSLK DLSREEFVRE ASTIFSHFYS IYPFCSGNGC VQRIFFEKMA EAAGHRLDFS VITKKRWQYA RYAALLPTRG DKAIIHDLFE DISNPEKVNI LKEFIRYENM SIDIKDKFVV AAKAGFPYTG LCKGYSGDAV MLMIDDQYVL CKKDDIAPGT LKTLKLGDRI TLTVPRSKAI AEDVLIPEAR IAPLTEKEII SRIEKYGSIQ GITRKIRALS KCVYGSSKML DNDINLILVD SGLGEQHIRQ HVSLLRYQLV GVTVLGVDNP ARKQARIDYF KLEQALDDYI SAVIYTRQIV LKGHKEQQER GERMVKMPSQ AVQNIFSLSE EDQIKALNGN PSLHEEIKDL VNKVKDRLTS WERQVLKEEC CVQLADSIGI SLSRAREVTA IVKNAHKVCQ LTTNREHEPK AMNYNLIEL
NT Sequence
gtggcaggat atgcatgcag gcattataca ttggcgcaat ttaatgcttt aatggatgct gatagagaaa aagtaatgct gagcaatcaa aaaaagggat ggaattataa ttcttctgcc aagataagaa ataaatatgg tttgcagggt atagattttg agaaaagatg tgtgcatgat gtaatacaag cgagtgttaa tctgaaagaa gagccactgc cagaaaaatt tgattcttct tatctaaaat atcttcataa gcgcttgttt gagaatacat ttgaatgggc tggttatacc cgggagtttc catttacatt ttcggatggt gcagttaggg aagatttaac aataacaata cctgacttgg attactctat aggcaatgat aaaattacaa acggtttaaa ggaaattgat cgaatacttg ccgaaaaaaa tagtttgaaa gatttatcac gtgaagaatt tgttcgtgaa gcatcaacaa tattttctca cttttatagt atatatcctt tctgtagtgg aaatggatgt gtacaacgta tattttttga aaaaatggca gaggccgcag ggcatagact tgacttctca gttataacaa agaaacgttg gcaatatgct cgctatgccg cattattacc tactagaggt gataaagcta ttatacacga tctttttgaa gatatctcta atccagaaaa agtaaatatt ttaaaggaat tcatacgata tgaaaatatg tccatagata tcaaagataa gtttgttgta gcagcaaaag caggttttcc ttatacgggt ctctgtaaag gttatagcgg agatgctgtt atgcttatga tagatgatca gtatgtttta tgcaaaaaag atgatattgc accaggaaca ctaaagacat tgaagcttgg tgataggatt actcttacag ttccaaggtc caaagcaatt gctgaagatg ttcttatccc agaagcaaga atagcccctt tgacggaaaa agaaattatt tcaagaattg aaaaatatgg ctccatccaa gggattacac gaaaaatcag agctttatca aaatgtgttt atggaagctc gaaaatgtta gataatgata taaatctgat acttgtagat tcaggtctcg gtgaacaaca tatcaggcag catgtgagtc ttttaagata tcagcttgta ggtgtgacag tattaggtgt cgacaatcca gcgcgcaaac aagctaggat agattacttt aagctcgagc aagcattgga tgattatata agtgctgtaa tttatactag acaaatagta ctgaaagggc acaaagaaca acaagaacgt ggcgagcgaa tggtaaaaat gcctagtcaa gcagtgcaaa atattttttc tttatctgaa gaagatcaga tcaaagcttt aaatggcaat ccttcccttc acgaagagat taaagatctt gtgaataaag tcaaagatcg ccttacatca tgggagcgtc aagtacttaa ggaagaatgt tgtgttcagt tggctgatag tataggcata tcactaagta gagcgcggga agttacagca attgttaaga acgctcataa agtatgtcag ctaacaacaa acagagagca tgagccgaaa gcgatgaact ataacctgat agaactctaa
Details for BasaA.17330.j.B1.GE35710
HARVESTED ON: 7/10/2012
SEQUENCED ON: 2/22/2016
EXPECTED MW: 65kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 52
Validated AA Sequence
MAHHHHHHMV AGYACRHYTL AQFNALMDAD REKVMLSNQK KGWNYNSSAK IRNKYGLQGI DFEKRCVHDV IQASVNLKEE PLPEKFDSSY LKYLHKRLFE NTFEWAGYTR EFPFTFSDGA VREDLTITIP DLDYSIGNDK ITNGLKEIDR ILAEKNSLKD LSREEFVREA STIFSHFYSI YPFCSGNGCV QRIFFEKMAE AAGHRLDFSV ITKKRWQYAR YAALLPTRGD KAIIHDLFED ISNPEKVNIL KEFIRYENMS IDIKDKFVVA AKAXFPYTGL CKGYSGXAVM XXIDDQYVLC K
Validated NT Sequence
atggctcacc accaccacca ccatatggtg gcaggatatg catgcaggca ttatacattg gcgcaattta atgctttaat ggatgctgat agagaaaaag taatgctgag caatcaaaaa aagggatgga attataattc ttctgccaag ataagaaata aatatggttt gcagggtata gattttgaga aaagatgtgt gcatgatgta atacaagcga gtgttaatct gaaagaagag ccactgccag aaaaatttga ttcttcttat ctaaaatatc ttcataagcg cttgtttgag aatacatttg aatgggctgg ttatacccgg gagtttccat ttacattttc ggatggtgca gttagggaag atttaacaat aacaatacct gacttggatt actctatagg caatgataaa attacaaacg gtttaaagga aattgatcga atacttgccg aaaaaaatag tttgaaagat ttatcacgtg aagaatttgt tcgtgaagca tcaacaatat tttctcactt ttatagtata tatcctttct gtagtggaaa tggatgtgta caacgtatat tttttgaaaa aatggcagag gccgcagggc atagacttga cttctcagtt ataacaaaga aacgttggca atatgctcgc tatgccgcat tattacctac tagaggtgat aaagctatta tacacgatct ttttgaagat atctctaatc cagaaaaagt aaatatttta aaggaattca tacgatatga aaatatgtcc atagatatca aagataagtt tgttgtagca gcnaaagcng nntttcctta tacgggtctc tgtaaaggnt atagcggaga ngctgttatg cntntgatag atgatcagta tgttttatgc aaaaa
Expected Protein Sequence
MAHHHHHHMV AGYACRHYTL AQFNALMDAD REKVMLSNQK KGWNYNSSAK IRNKYGLQGI DFEKRCVHDV IQASVNLKEE PLPEKFDSSY LKYLHKRLFE NTFEWAGYTR EFPFTFSDGA VREDLTITIP DLDYSIGNDK ITNGLKEIDR ILAEKNSLKD LSREEFVREA STIFSHFYSI YPFCSGNGCV QRIFFEKMAE AAGHRLDFSV ITKKRWQYAR YAALLPTRGD KAIIHDLFED ISNPEKVNIL KEFIRYENMS IDIKDKFVVA AKAGFPYTGL CKGYSGDAVM LMIDDQYVLC KKDDIAPGTL KTLKLGDRIT LTVPRSKAIA EDVLIPEARI APLTEKEIIS RIEKYGSIQG ITRKIRALSK CVYGSSKMLD NDINLILVDS GLGEQHIRQH VSLLRYQLVG VTVLGVDNPA RKQARIDYFK LEQALDDYIS AVIYTRQIVL KGHKEQQERG ERMVKMPSQA VQNIFSLSEE DQIKALNGNP SLHEEIKDLV NKVKDRLTSW ERQVLKEECC VQLADSIGIS LSRAREVTAI VKNAHKVCQL TTNREHEPKA MNYNLIEL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgg caggatatgc atgcaggcat tatacattgg cgcaatttaa tgctttaatg gatgctgata gagaaaaagt aatgctgagc aatcaaaaaa agggatggaa ttataattct tctgccaaga taagaaataa atatggtttg cagggtatag attttgagaa aagatgtgtg catgatgtaa tacaagcgag tgttaatctg aaagaagagc cactgccaga aaaatttgat tcttcttatc taaaatatct tcataagcgc ttgtttgaga atacatttga atgggctggt tatacccggg agtttccatt tacattttcg gatggtgcag ttagggaaga tttaacaata acaatacctg acttggatta ctctataggc aatgataaaa ttacaaacgg tttaaaggaa attgatcgaa tacttgccga aaaaaatagt ttgaaagatt tatcacgtga agaatttgtt cgtgaagcat caacaatatt ttctcacttt tatagtatat atcctttctg tagtggaaat ggatgtgtac aacgtatatt ttttgaaaaa atggcagagg ccgcagggca tagacttgac ttctcagtta taacaaagaa acgttggcaa tatgctcgct atgccgcatt attacctact agaggtgata aagctattat acacgatctt tttgaagata tctctaatcc agaaaaagta aatattttaa aggaattcat acgatatgaa aatatgtcca tagatatcaa agataagttt gttgtagcag caaaagcagg ttttccttat acgggtctct gtaaaggtta tagcggagat gctgttatgc ttatgataga tgatcagtat gttttatgca aaaaagatga tattgcacca ggaacactaa agacattgaa gcttggtgat aggattactc ttacagttcc aaggtccaaa gcaattgctg aagatgttct tatcccagaa gcaagaatag cccctttgac ggaaaaagaa attatttcaa gaattgaaaa atatggctcc atccaaggga ttacacgaaa aatcagagct ttatcaaaat gtgtttatgg aagctcgaaa atgttagata atgatataaa tctgatactt gtagattcag gtctcggtga acaacatatc aggcagcatg tgagtctttt aagatatcag cttgtaggtg tgacagtatt aggtgtcgac aatccagcgc gcaaacaagc taggatagat tactttaagc tcgagcaagc attggatgat tatataagtg ctgtaattta tactagacaa atagtactga aagggcacaa agaacaacaa gaacgtggcg agcgaatggt aaaaatgcct agtcaagcag tgcaaaatat tttttcttta tctgaagaag atcagatcaa agctttaaat ggcaatcctt cccttcacga agagattaaa gatcttgtga ataaagtcaa agatcgcctt acatcatggg agcgtcaagt acttaaggaa gaatgttgtg ttcagttggc tgatagtata ggcatatcac taagtagagc gcgggaagtt acagcaattg ttaagaacgc tcataaagta tgtcagctaa caacaaacag agagcatgag ccgaaagcga tgaactataa cctgatagaa ctctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BasaA.17330.j.B3.GE37355
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK KGWNYNSSAK IRNKYGLQGI DFEKRCVHDV IQASVNLKEE PLPEKFDSSY LKYLHKRLFE NTFEWAGYTR EFPFTFSDGA VREDLTITIP DLDYSIGNDK ITNGLKEIDR ILAEKNSLKD LSREEFVREA STIFSHFYSI YPFCSGNGCV QRIFFEKMAE AAGHRLDFSV ITKKRWQYAR YAALLPTRGD KAIIHDLFED ISNPEKVNIL KEFIRYENMS IDIKDKFVVA AKAGFPYTGL CKGYSGDAVM LMIDDQYVLC KKDDIAPGTL KTLKLXIGLL LHK
Validated NT Sequence
ttgnttaact ttaagaagga ganataccat ggctcaccac caccaccacc atatgaaaaa gggatggaat tataattctt ctgccaagat aagaaataaa tatggtttgc agggtataga ttttgagaaa agatgtgtgc atgatgtaat acaagcgagt gttaatctga aagaagagcc actgccagaa aaatttgatt cttcttatct aaaatatctt cataagcgct tgtttgagaa tacatttgaa tgggctggtt atacccggga gtttccattt acattttcgg atggtgcagt tagggaagat ttaacaataa caatacctga cttggattac tctataggca atgataaaat tacaaacggt ttaaaggaaa ttgatcgaat acttgccgaa aaaaatagtt tgaaagattt atcacgtgaa gaatttgttc gtgaagcatc aacaatattt tctcactttt atagtatata tcctttctgt agtggaaatg gatgtgtaca acgtatattt tttgaaaaaa tggcagaggc cgcagggcat agacttgact tctcagttat aacaaagaaa cgttggcaat atgctcgcta tgccgcatta ttacctacta gaggtgataa agctattata cacgatcttt ttgaagatat ctctaatcca gaaaaagtaa atattttaaa ggaattcata cgatatgaaa atatgtccat agatatcaaa gataagtttg ttgtagcagc aaaagcaggt tttccttata cgggtctctg taaaggttat agcggagatg ctgttatgct tatgatagat gatcagtatg ttttatgcaa aaaagatgat attgcaccag gaacactaaa gacattgaag cttnnnatag gattactctt acataagtga gtaananagg anccnnnnnn ncccccccnn nnnnnannnn nnnnnnnnnn nnaaaanaaa nannnnnnnn nntnnnnnnn nnnnnnt
Expected Protein Sequence
MAHHHHHHKK GWNYNSSAKI RNKYGLQGID FEKRCVHDVI QASVNLKEEP LPEKFDSSYL KYLHKRLFEN TFEWAGYTRE FPFTFSDGAV REDLTITIPD LDYSIGNDKI TNGLKEIDRI LAEKNSLKDL SREEFVREAS TIFSHFYSIY PFCSGNGCVQ RIFFEKMAEA AGHRLDFSVI TKKRWQYARY AALLPTRGDK AIIHDLFEDI SNPEKVNILK EFIRYENMSI DIKDKFVVAA KAGFPYTGLC KGYSGDAVML MIDDQYVLCK KDDIAPGTLK TLKLGDRITL T
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaaaagg gatggaatta taattcttct gccaagataa gaaataaata tggtttgcag ggtatagatt ttgagaaaag atgtgtgcat gatgtaatac aagcgagtgt taatctgaaa gaagagccac tgccagaaaa atttgattct tcttatctaa aatatcttca taagcgcttg tttgagaata catttgaatg ggctggttat acccgggagt ttccatttac attttcggat ggtgcagtta gggaagattt aacaataaca atacctgact tggattactc tataggcaat gataaaatta caaacggttt aaaggaaatt gatcgaatac ttgccgaaaa aaatagtttg aaagatttat cacgtgaaga atttgttcgt gaagcatcaa caatattttc tcacttttat agtatatatc ctttctgtag tggaaatgga tgtgtacaac gtatattttt tgaaaaaatg gcagaggccg cagggcatag acttgacttc tcagttataa caaagaaacg ttggcaatat gctcgctatg ccgcattatt acctactaga ggtgataaag ctattataca cgatcttttt gaagatatct ctaatccaga aaaagtaaat attttaaagg aattcatacg atatgaaaat atgtccatag atatcaaaga taagtttgtt gtagcagcaa aagcaggttt tccttatacg ggtctctgta aaggttatag cggagatgct gttatgctta tgatagatga tcagtatgtt ttatgcaaaa aagatgatat tgcaccagga acactaaaga cattgaagct tggtgatagg attactctta catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BasaA.17330.j.B4.GE37356
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 17kDa
OBSERVED MW: 16kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHLI PEARIAPLTE KEIISRIEKY GSIQGITRKI RALSKCVYGS SKMLDNDINL ILVDSGLGEQ HIRQHVSLLR YQLVGVTVLG VDNPARKQAR IDYFKLEQAL DDYISAVIYT RQIVLKGHKE QQERGERMVK MPSQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcttatcc cagaagcaag aatagcccct ttgacggaaa aagaaattat ttcaagaatt gaaaaatatg gctccatcca agggattaca cgaaaaatca gagctttatc aaaatgtgtt tatggaagct cgaaaatgtt agataatgat ataaatctga tacttgtaga ttcaggtctc ggtgaacaac atatcaggca gcatgtgagt cttttaagat atcagcttgt aggtgtgaca gtattaggtg tcgacaatcc agcgcgcaaa caagctagga tagattactt taagctcgag caagcattgg atgattatat aagtgctgta atttatacta gacaaatagt actgaaaggg cacaaagaac aacaagaacg tggcgagcga atggtaaaaa tgcctagtca atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BasaA.17330.j.B4.PS01938
PURIFICATION DATe: 11/21/2013
CONCENTRATION: 42mg/ml
OBSERVED MW: 16kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHLI PEARIAPLTE KEIISRIEKY GSIQGITRKI RALSKCVYGS SKMLDNDINL ILVDSGLGEQ HIRQHVSLLR YQLVGVTVLG VDNPARKQAR IDYFKLEQAL DDYISAVIYT RQIVLKGHKE QQERGERMVK MPSQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTTATCC CAGAAGCAAG AATAGCCCCT TTGACGGAAA AAGAAATTAT TTCAAGAATT GAAAAATATG GCTCCATCCA AGGGATTACA CGAAAAATCA GAGCTTTATC AAAATGTGTT TATGGAAGCT CGAAAATGTT AGATAATGAT ATAAATCTGA TACTTGTAGA TTCAGGTCTC GGTGAACAAC ATATCAGGCA GCATGTGAGT CTTTTAAGAT ATCAGCTTGT AGGTGTGACA GTATTAGGTG TCGACAATCC AGCGCGCAAA CAAGCTAGGA TAGATTACTT TAAGCTCGAG CAAGCATTGG ATGATTATAT AAGTGCTGTA ATTTATACTA GACAAATAGT ACTGAAAGGG CACAAAGAAC AACAAGAACG TGGCGAGCGA ATGGTAAAAA TGCCTAGTCA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT