BasaA.17330.g

Bep10 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BasaA.17330.g
ORGANISM: Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BasaA.17330.g.B1.GE35707 full length 1 557
BasaA.17330.g.B2.GE36619 FIC 20 250
BasaA.17330.g.B3.GE37038 FIC and OB-Fold 20 309
BasaA.17330.g.B4.GE37039 BID 318 456
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: E6YS53

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIRDSEAFK KVEQSLLQQS SNYTYANSGS GNSNTLKNKF GIQDYKNFQI KCAHAVAKEI VNVYQEPAPE KFDHSYLKQL HKRLFKKVFD WAGETRDASV KMSDGTIANM VTTSQNPLFA SNNDIKKCLE EFNEKLAQRK KSQDTLQEKP EEKLTDSLTT VYALLNHARP FVDGNGVTQR LFMNKLSQAE DQKLDFSVVT QKRMEDAIVN AANDNLAPMK HLFEDASDPQ KIQTLKTALT GMNSGDIEDL TQGIVTTPIP GKVYTGVYES RQLDTIVIKT QNSVIVCNKY DIPREEFQKL RLGDTVSFKA LNNLDDVLIP GRELSDLTNF KVMEKTFYTP SVQQQRKEVE RLARLVFGNR ETLSNYLERV HENPSSATML ANRIVYAHKS ISKLAGFGAY GINSPKRNRA EKNLIALSEA VRKYGDCLKS SKEEVLQEHR QEQYRIAKDV HLPSKDLARF LNMSPDERGK LPITEAATLS KEAAQFLKQI YSRLSADEQK MVNNNDHKSF AKSIGIPENK AQQVMETVKQ VKAIQEPTNV IKPLMKHSPK QAMAIAN
NT Sequence
atgtcgataa gagactctga agcttttaaa aaagtagaac aatcgctact gcaacaaagt tctaattaca cctatgctaa ttctggttct ggaaatagca atacattaaa aaataaattt gggatacaag attataagaa ctttcagata aagtgtgctc atgcagtggc aaaagagatt gttaatgtgt atcaagaacc agcgccagaa aagtttgacc actcttatct aaaacagctt cacaaacgtt tgtttaaaaa agtatttgac tgggctggag aaacgcgcga cgcttcagtt aagatgtcag atggtacaat tgctaacatg gtaacgacta gtcaaaatcc tctttttgca tctaataatg acatcaaaaa atgcttagag gagttcaatg aaaagcttgc tcaaagaaag aaatctcaag atactttaca agagaaacca gaggaaaaac ttactgattc tctaacaacc gtctatgcat tacttaacca tgcacgtcct ttcgtagatg gcaatggggt tacacaacgg ttatttatga ataaactctc tcaagctgaa gatcaaaagc ttgatttttc agttgtgaca caaaaacgta tggaagatgc tattgttaat gcagcaaatg acaatttagc gcctatgaaa catctgtttg aagatgcttc tgatccacaa aaaattcaaa ctttgaaaac agctctaact ggaatgaatt ctggagatat agaagatctt acacagggta ttgttacaac accaatccca gggaaagtct atacaggtgt ttatgaaagt cgtcaattgg acactattgt gataaaaacg cagaattctg tgattgtatg caataaatat gatattccaa gagaggaatt ccagaaatta aggttaggtg atacagtcag ctttaaagct cttaacaacc ttgacgatgt tctgatccca ggacgagaat tgtctgattt aacaaacttt aaagttatgg aaaagacttt ctacactcct tctgttcaac agcaaaggaa agaagttgag cgtttagcaa gacttgtatt tggaaaccgt gagacattat ctaattacct agagcgagtt catgagaatc caagttcagc aacaatgctt gctaatagaa ttgtatacgc ccataaatct atctctaagc ttgcgggttt tggagcatat gggataaaca gtccaaaacg taacagagct gaaaaaaatc ttatagccct tagcgaggca gttagaaaat atggagattg cctaaaatct tctaaggagg aagttcttca agagcataga caagaacaat accgcattgc aaaagatgta catttaccta gcaaagattt ggcgcgtttt cttaatatgt caccagatga gcgtggaaag cttcctatta ctgaagctgc tactctcagc aaagaagctg cccaattttt aaaacaaatt tattctcgtc tttcagcaga tgagcaaaaa atggttaata ataatgatca taaatcattt gctaaaagta ttggtattcc agaaaacaaa gcacaacagg ttatggagac cgttaaacag gttaaagcta tacaagagcc gactaatgta attaaacctt taatgaagca ttcccccaaa caagcaatgg ctatagctaa ttag
Details for BasaA.17330.g.B1.GE35707
HARVESTED ON: 7/10/2012
SEQUENCED ON: 2/22/2016
EXPECTED MW: 64kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 56
Validated AA Sequence
MAHHHHHHMS IRDSEAFKKV EQSLLQQSSN YTYANSGSGN SNTLKNKFGI QDYKNFQIKC AHAVAKEIVN VYQEPAPEKF DHSYLKQLHK RLFKKVFDWA GETRDASVKM SDGTIANMVT TSQNPLFASN NDIKKCLEEF NEKLAQRKKS QDTLQEKPEE KLTDSLTTVY ALLNHARPFV DGNGVTQRLF MNKLSQAEDQ KLDFSVVTQK RMEDAIVNAA NDNLAPMKHL FEDASDPQKI QTLKTALTGM NSGDIEXLTQ GIVTTPIPGK VYTGVYESRQ LDTIVIKTQX SVIVCNKYDI PRXXXXXLRX XXXVSFK
Validated NT Sequence
atggctcacc accaccacca ccatatgtcg ataagagact ctgaagcttt taaaaaagta gaacaatcgc tactgcaaca aagttctaat tacacctatg ctaattctgg ttctggaaat agcaatacat taaaaaataa atttgggata caagattata agaactttca gataaagtgt gctcatgcag tggcaaaaga gattgttaat gtgtatcaag aaccagcgcc agaaaagttt gaccactctt atctaaaaca gcttcacaaa cgtttgttta aaaaagtatt tgactgggct ggagaaacgc gcgacgcttc agttaagatg tcagatggta caattgctaa catggtaacg actagtcaaa atcctctttt tgcatctaat aatgacatca aaaaatgctt agaggagttc aatgaaaagc ttgctcaaag aaagaaatct caagatactt tacaagagaa accagaggaa aaacttactg attctctaac aaccgtctat gcattactta accatgcacg tcctttcgta gatggcaatg gggttacaca acggttattt atgaataaac tctctcaagc tgaagatcaa aagcttgatt tttcagttgt gacacaaaaa cgtatggaag atgctattgt taatgcagca aatgacaatt tagcgcctat gaaacatctg tttgaagatg cttctgatcc acaaaaaatt caaactttga aaacagctct aactggaatg aattctggag atatagaagn tcttacacag ggtattgtta caacaccaat cccagggaaa gtctatacag gtgtttatga aagtcgtcaa ttggacacta ttgtgataaa aacgcagann tctgtgattg tatgcaataa atatgatatt ccaagagann nnnnnnnnnn attaaggttn nnngnnnnag tcagctttaa agc
Expected Protein Sequence
MAHHHHHHMS IRDSEAFKKV EQSLLQQSSN YTYANSGSGN SNTLKNKFGI QDYKNFQIKC AHAVAKEIVN VYQEPAPEKF DHSYLKQLHK RLFKKVFDWA GETRDASVKM SDGTIANMVT TSQNPLFASN NDIKKCLEEF NEKLAQRKKS QDTLQEKPEE KLTDSLTTVY ALLNHARPFV DGNGVTQRLF MNKLSQAEDQ KLDFSVVTQK RMEDAIVNAA NDNLAPMKHL FEDASDPQKI QTLKTALTGM NSGDIEDLTQ GIVTTPIPGK VYTGVYESRQ LDTIVIKTQN SVIVCNKYDI PREEFQKLRL GDTVSFKALN NLDDVLIPGR ELSDLTNFKV MEKTFYTPSV QQQRKEVERL ARLVFGNRET LSNYLERVHE NPSSATMLAN RIVYAHKSIS KLAGFGAYGI NSPKRNRAEK NLIALSEAVR KYGDCLKSSK EEVLQEHRQE QYRIAKDVHL PSKDLARFLN MSPDERGKLP ITEAATLSKE AAQFLKQIYS RLSADEQKMV NNNDHKSFAK SIGIPENKAQ QVMETVKQVK AIQEPTNVIK PLMKHSPKQA MAIAN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcga taagagactc tgaagctttt aaaaaagtag aacaatcgct actgcaacaa agttctaatt acacctatgc taattctggt tctggaaata gcaatacatt aaaaaataaa tttgggatac aagattataa gaactttcag ataaagtgtg ctcatgcagt ggcaaaagag attgttaatg tgtatcaaga accagcgcca gaaaagtttg accactctta tctaaaacag cttcacaaac gtttgtttaa aaaagtattt gactgggctg gagaaacgcg cgacgcttca gttaagatgt cagatggtac aattgctaac atggtaacga ctagtcaaaa tcctcttttt gcatctaata atgacatcaa aaaatgctta gaggagttca atgaaaagct tgctcaaaga aagaaatctc aagatacttt acaagagaaa ccagaggaaa aacttactga ttctctaaca accgtctatg cattacttaa ccatgcacgt cctttcgtag atggcaatgg ggttacacaa cggttattta tgaataaact ctctcaagct gaagatcaaa agcttgattt ttcagttgtg acacaaaaac gtatggaaga tgctattgtt aatgcagcaa atgacaattt agcgcctatg aaacatctgt ttgaagatgc ttctgatcca caaaaaattc aaactttgaa aacagctcta actggaatga attctggaga tatagaagat cttacacagg gtattgttac aacaccaatc ccagggaaag tctatacagg tgtttatgaa agtcgtcaat tggacactat tgtgataaaa acgcagaatt ctgtgattgt atgcaataaa tatgatattc caagagagga attccagaaa ttaaggttag gtgatacagt cagctttaaa gctcttaaca accttgacga tgttctgatc ccaggacgag aattgtctga tttaacaaac tttaaagtta tggaaaagac tttctacact ccttctgttc aacagcaaag gaaagaagtt gagcgtttag caagacttgt atttggaaac cgtgagacat tatctaatta cctagagcga gttcatgaga atccaagttc agcaacaatg cttgctaata gaattgtata cgcccataaa tctatctcta agcttgcggg ttttggagca tatgggataa acagtccaaa acgtaacaga gctgaaaaaa atcttatagc ccttagcgag gcagttagaa aatatggaga ttgcctaaaa tcttctaagg aggaagttct tcaagagcat agacaagaac aataccgcat tgcaaaagat gtacatttac ctagcaaaga tttggcgcgt tttcttaata tgtcaccaga tgagcgtgga aagcttccta ttactgaagc tgctactctc agcaaagaag ctgcccaatt tttaaaacaa atttattctc gtctttcagc agatgagcaa aaaatggtta ataataatga tcataaatca tttgctaaaa gtattggtat tccagaaaac aaagcacaac aggttatgga gaccgttaaa caggttaaag ctatacaaga gccgactaat gtaattaaac ctttaatgaa gcattccccc aaacaagcaa tggctatagc taattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BasaA.17330.g.B2.GE36619
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS SNYTYANSGS GNSNTLKNKF GIQDYKNFQI KCAHAVAKEI VNVYQEPAPE KFDHSYLKQL HKRLFKKVFD WAGETRDASV KMSDGTIANM VTTSQNPLFA SNNDIKKCLE EFNEKLAQRK KSQDTLQEKP EEKLTDSLTT VYALLNHARP FVDGNGVTQR LFMNKLSQAE DQKLDFSVVT QKRMEDAIVN AANDNLAPMK HLFEDASDPQ KIQTLKTALT GMNSGDIEXL
Validated NT Sequence
tttgnnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgagttc taattacacc tatgctaatt ctggttctgg aaatagcaat acattaaaaa ataaatttgg gatacaagat tataagaact ttcagataaa gtgtgctcat gcagtggcaa aagagattgt taatgtgtat caagaaccag cgccagaaaa gtttgaccac tcttatctaa aacagcttca caaacgtttg tttaaaaaag tatttgactg ggctggagaa acgcgcgacg cttcagttaa gatgtcagat ggtacaattg ctaacatggt aacgactagt caaaatcctc tttttgcatc taataatgac atcaaaaaat gcttagagga gttcaatgaa aagcttgctc aaagaaagaa atctcaagat actttacaag agaaaccaga ggaaaaactt actgattctc taacaaccgt ctatgcatta cttaaccatg cacgtccttt cgtagatggc aatggggtta cacaacggtt atttatgaat aaactctctc aagctgaaga tcaaaagctt gatttttcag ttgtgacaca aaaacgtatg gaagatgcta ttgttaatgc agcaaatgac aatttagcgc ctatgaaaca tctgtttgaa gatgcttctg atccacaaaa aattcaaact ttgaaaacag ctctaactgg aatgaattct ggagatatag aagancttta agtgagtaag ataggatccg gntccccnnn nnannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc nntgnnnnan tntnatttcn nncnnnnnnn ctnncgnnnn nnnntnnnnn ctttnnannn ntgcgnnnnn nnnnnngnnn nnnnntcnnn nncgnnnnct gtg
Expected Protein Sequence
MAHHHHHHMS SNYTYANSGS GNSNTLKNKF GIQDYKNFQI KCAHAVAKEI VNVYQEPAPE KFDHSYLKQL HKRLFKKVFD WAGETRDASV KMSDGTIANM VTTSQNPLFA SNNDIKKCLE EFNEKLAQRK KSQDTLQEKP EEKLTDSLTT VYALLNHARP FVDGNGVTQR LFMNKLSQAE DQKLDFSVVT QKRMEDAIVN AANDNLAPMK HLFEDASDPQ KIQTLKTALT GMNSGDIEDL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtt ctaattacac ctatgctaat tctggttctg gaaatagcaa tacattaaaa aataaatttg ggatacaaga ttataagaac tttcagataa agtgtgctca tgcagtggca aaagagattg ttaatgtgta tcaagaacca gcgccagaaa agtttgacca ctcttatcta aaacagcttc acaaacgttt gtttaaaaaa gtatttgact gggctggaga aacgcgcgac gcttcagtta agatgtcaga tggtacaatt gctaacatgg taacgactag tcaaaatcct ctttttgcat ctaataatga catcaaaaaa tgcttagagg agttcaatga aaagcttgct caaagaaaga aatctcaaga tactttacaa gagaaaccag aggaaaaact tactgattct ctaacaaccg tctatgcatt acttaaccat gcacgtcctt tcgtagatgg caatggggtt acacaacggt tatttatgaa taaactctct caagctgaag atcaaaagct tgatttttca gttgtgacac aaaaacgtat ggaagatgct attgttaatg cagcaaatga caatttagcg cctatgaaac atctgtttga agatgcttct gatccacaaa aaattcaaac tttgaaaaca gctctaactg gaatgaattc tggagatata gaagatcttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BasaA.17330.g.B3.GE37038
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS SNYTYANSGS GNSNTLKNKF GIQDYKNFQI KCAHAVAKEI VNVYQEPAPE KFDHSYLKQL HKRLFKKVFD WAGETRDASV KMSDGTIANM VTTSQNPLFA SNNDIKKCLE EFNEKLAQRK KSQDTLQEKP EEKLTDSLTT VYALLNHARP FVDGNGVTQR LFMNKLSQAE DQKLDFSVVT QKRMEDAIVN AANDNLAPMK HLFEDASDPQ KIQTLKTALT GMNSGDIEDL TQGIVTTPIP GKVYTGVYES RQLXTIVIKT XNSVXVCNKY DIPREEFQKX XVGDTVSFK
Validated NT Sequence
ttgtttactt taagaaggag anataccatg gctcaccacc accaccacca tatgagttct aattacacct atgctaattc tggttctgga aatagcaata cattaaaaaa taaatttggg atacaagatt ataagaactt tcagataaag tgtgctcatg cagtggcaaa agagattgtt aatgtgtatc aagaaccagc gccagaaaag tttgaccact cttatctaaa acagcttcac aaacgtttgt ttaaaaaagt atttgactgg gctggagaaa cgcgcgacgc ttcagttaag atgtcagatg gtacaattgc taacatggta acgactagtc aaaatcctct ttttgcatct aataatgaca tcaaaaaatg cttagaggag ttcaatgaaa agcttgctca aagaaagaaa tctcaagata ctttacaaga gaaaccagag gaaaaactta ctgattctct aacaaccgtc tatgcattac ttaaccatgc acgtcctttc gtagatggca atggggttac acaacggtta tttatgaata aactctctca agctgaagat caaaagcttg atttttcagt tgtgacacaa aaacgtatgg aagatgctat tgttaatgca gcaaatgaca atttagcgcc tatgaaacat ctgtttgaag atgcttctga tccacaaaaa attcaaactt tgaaaacagc tctaactgga atgaattctg gagatataga agatcttaca cagggtattg ttacaacacc aatcccaggg aaagtctata caggtgttta tgaaagtcgt caattgnaca ctattgtgat aaaaacgcan aattctgtga ntgtatgcaa taaatatgat attccaagag aggaattcca gaaattnang gtaggtgata cagtcagctt taaataagtg agtaanatag gatccggctg cnannnnncc ccnnaa
Expected Protein Sequence
MAHHHHHHSS NYTYANSGSG NSNTLKNKFG IQDYKNFQIK CAHAVAKEIV NVYQEPAPEK FDHSYLKQLH KRLFKKVFDW AGETRDASVK MSDGTIANMV TTSQNPLFAS NNDIKKCLEE FNEKLAQRKK SQDTLQEKPE EKLTDSLTTV YALLNHARPF VDGNGVTQRL FMNKLSQAED QKLDFSVVTQ KRMEDAIVNA ANDNLAPMKH LFEDASDPQK IQTLKTALTG MNSGDIEDLT QGIVTTPIPG KVYTGVYESR QLDTIVIKTQ NSVIVCNKYD IPREEFQKLR LGDTVSFK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagttcta attacaccta tgctaattct ggttctggaa atagcaatac attaaaaaat aaatttggga tacaagatta taagaacttt cagataaagt gtgctcatgc agtggcaaaa gagattgtta atgtgtatca agaaccagcg ccagaaaagt ttgaccactc ttatctaaaa cagcttcaca aacgtttgtt taaaaaagta tttgactggg ctggagaaac gcgcgacgct tcagttaaga tgtcagatgg tacaattgct aacatggtaa cgactagtca aaatcctctt tttgcatcta ataatgacat caaaaaatgc ttagaggagt tcaatgaaaa gcttgctcaa agaaagaaat ctcaagatac tttacaagag aaaccagagg aaaaacttac tgattctcta acaaccgtct atgcattact taaccatgca cgtcctttcg tagatggcaa tggggttaca caacggttat ttatgaataa actctctcaa gctgaagatc aaaagcttga tttttcagtt gtgacacaaa aacgtatgga agatgctatt gttaatgcag caaatgacaa tttagcgcct atgaaacatc tgtttgaaga tgcttctgat ccacaaaaaa ttcaaacttt gaaaacagct ctaactggaa tgaattctgg agatatagaa gatcttacac agggtattgt tacaacacca atcccaggga aagtctatac aggtgtttat gaaagtcgtc aattggacac tattgtgata aaaacgcaga attctgtgat tgtatgcaat aaatatgata ttccaagaga ggaattccag aaattaaggt taggtgatac agtcagcttt aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BasaA.17330.g.B4.GE37039
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHLI PGRELSDLTN FKVMEKTFYT PSVQQQRKEV ERLARLVFGN RETLSNYLER VHENPSSATM LANRIVYAHK SISKLAGFGA YGINSPKRNR AEKNLIALSE AVRKYGDCLK SSKEEVLQEH RQEQYRIAKD VHLPSKD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctgatcc caggacgaga attgtctgat ttaacaaact ttaaagttat ggaaaagact ttctacactc cttctgttca acagcaaagg aaagaagttg agcgtttagc aagacttgta tttggaaacc gtgagacatt atctaattac ctagagcgag ttcatgagaa tccaagttca gcaacaatgc ttgctaatag aattgtatac gcccataaat ctatctctaa gcttgcgggt tttggagcat atgggataaa cagtccaaaa cgtaacagag ctgaaaaaaa tcttatagcc cttagcgagg cagttagaaa atatggagat tgcctaaaat cttctaagga ggaagttctt caagagcata gacaagaaca ataccgcatt gcaaaagatg tacatttacc tagcaaagat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat