BasaA.17330.f

Bep7 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BasaA.17330.f
ORGANISM: Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BasaA.17330.f.B2.GE36617 FIC 27 258
BasaA.17330.f.B3.GE36618 FIC and OB-Fold 27 314
BasaA.17330.f.B4.GE37037 BID 325 463
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BasaA.17330.f.B4.PW37424 BID 325 463

External Resources

RESOURCE REFERENCE ID
UniProt: E6YQP8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKTNLKSLK KVFKPLTKKI EPLNLLSCDY TYPDSNTLIN KYGTKDSRVL EAMCAADAER VAVHLRQEAL PEKFDSTYLK YLHKRLFESI FEWAGCTRNL TFKFENGTTA SISKMQIPNS DAFFKDSKKV GKSLHKFDQI LSDKNNLQNL SRKEFVEESV KLFSFLNYIH PFRDGNGHAQ QMFFEKLAQA AGHQLDFSVV TKKRMIHACN NTIPAKGNVN YEAMQHLFED ISNPEKTRLL KDYLCSASEH RRKLLNDQII IAPREDIDYT GIYKSSNADS IIIETVDLHI VCCKDYFAPE QLKALKFGDK ITFKIPINKN LNQVLIPAEK LTSLTLEEML KKVKDSAVLK EDQKEIKRLS KIVYGSSKVL DQSIDMINQE QEMSEKIAQQ ILEHPRSISK LAGFRIWFIK SPKRIRAERK LSLLSQKIKD YGYGVGLVKN MIIRNHEVEK KRLAQEVRLP SEAVQNILNM SQSERQKTLS GEDCVKIKKE LFDFLLKIDS RLTRDEAKAL RIGDYQTIAQ SINISEHKAK ILIETVRETK KVYRQLQQCR VDKLKQMAVA V
NT Sequence
atgaaaaaaa caaatttaaa atctctaaaa aaagttttta agcctttaac aaaaaaaata gaaccattaa acttattgtc ttgcgattat acttatcctg acagtaatac attaataaat aaatatggaa caaaagattc tcgggttctt gaagcaatgt gcgcagctga tgcagaaaga gtagctgttc atttgcgtca agaagcattg ccagagaagt ttgattccac ttatttaaaa tatcttcata aacgcttatt tgaaagcata ttcgaatggg caggctgtac tcgcaatctt acatttaaat ttgaaaatgg tacaaccgct agcatctcaa aaatgcaaat accaaattca gatgctttct tcaaagatag taaaaaagta ggaaaaagct tacataaatt tgatcaaata ctttctgaca aaaataattt gcaaaactta tcgcgcaaag aatttgtcga ggaatcagta aagctttttt cttttcttaa ctatatacat cccttcagag atggcaatgg gcatgcacaa caaatgtttt ttgaaaaact cgcacaagct gcaggtcatc agcttgattt ttcagttgtg acaaaaaaac gcatgataca tgcctgtaat aatacaatac ctgcaaaagg taatgtaaat tatgaagcaa tgcaacattt gttcgaagat atctccaatc cagaaaaaac gcgcctttta aaggactatt tatgttctgc atctgagcat agacgtaagc ttttgaatga tcaaattatt atagcaccac gtgaagatat agactatacg ggtatttata agagtagtaa tgcggattct attataattg aaacagtaga cttacatatc gtatgctgta aagattattt tgcaccagaa caacttaagg cattgaaatt tggtgataag attaccttta aaattccaat aaataaaaat ttaaatcaag ttcttatccc agcagaaaaa ctcacttctt tgacgttaga agaaatgctt aaaaaggtta aggacagtgc tgtgcttaaa gaagatcaaa aagaaatcaa gcgtttatca aaaattgttt atggaagttc aaaagtatta gatcaaagta tcgatatgat taatcaagag caagaaatga gtgaaaaaat tgcccaacaa attttagaac atcctcgatc tatttctaaa ctcgctggct ttagaatatg gtttataaag agcccaaagc gcataagggc tgaaagaaag ctttcattac ttagtcaaaa aattaaagat tacgggtatg gtgtaggact tgttaaaaat atgatcattc gaaatcatga agtagaaaaa aaacgccttg cacaagaagt aaggttgcct agtgaagcag tgcaaaatat ccttaacatg tctcaaagtg aaagacaaaa aactttaagc ggagaagatt gtgttaaaat caaaaaagag ctcttcgact ttttattgaa aatcgattct cgtcttacaa gggatgaggc taaagcgctc agaataggtg attatcaaac aatcgctcaa agtattaata tatcagaaca taaagcaaaa atacttatag aaactgttag agaaacaaaa aaagtgtatc gacaacttca acaatgcaga gtggataagt taaaacagat ggctgtggca gtctaa
Details for BasaA.17330.f.B2.GE36617
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 28kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS CDYTYPDSNT LINKYGTKDS RVLEAMCAAD AERVAVHLRQ EALPEKFDST YLKYLHKRLF ESIFEWAGCT RNLTFKFENG TTASISKMQI PNSDAFFKDS KKVGKSLHKF DQILSDKNNL QNLSRKEFVE ESVKLFSFLN YIHPFRDGNG HAQQMFFEKL AQAAGHQLDF SVVTKKRMIH ACNNTIPAKG NVNYEAMQHL FEDISNPEKT RLLKDYLCSA SEHRRKLLND Q
Validated NT Sequence
atcttactca cttattgatc attcaaaagc ttacgtctat gctcagatgc agaacataaa tagtccttta aaaggcgcgt tttttctgga ttggagatat cttcgaacaa atgttgcatt gcttcataat ttacattacc ttttgcaggt attgtattat tacaggcatg tatcatgcgt ttttttgtca caactgaaaa atcaagctga tgacctgcag cttgtgcgag tttttcaaaa aacatttgtt gtgcatgccc attgccatct ctgaagggat gtatatagtt aagaaaagaa aaaagcttta ctgattcctc gacaaattct ttgcgcgata agttttgcaa attatttttg tcagaaagta tttgatcaaa tttatgtaag ctttttccta cttttttact atctttgaag aaagcatctg aatttggtat ttgcattttt gagatgctag cggttgtacc attttcaaat ttaaatgtaa gattgcgagt acagcctgcc cattcgaata tgctttcaaa taagcgttta tgaagatatt ttaaataagt ggaatcaaac ttctctggca atgcttcttg acgcaaatga acagctactc tttctgcatc agctgcgcac attgcttcaa gaacccgaga atcttttgtt ccatatttat ttattaatgt attactgtca ggataagtat aatcgcaaga catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMS CDYTYPDSNT LINKYGTKDS RVLEAMCAAD AERVAVHLRQ EALPEKFDST YLKYLHKRLF ESIFEWAGCT RNLTFKFENG TTASISKMQI PNSDAFFKDS KKVGKSLHKF DQILSDKNNL QNLSRKEFVE ESVKLFSFLN YIHPFRDGNG HAQQMFFEKL AQAAGHQLDF SVVTKKRMIH ACNNTIPAKG NVNYEAMQHL FEDISNPEKT RLLKDYLCSA SEHRRKLLND Q
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt gcgattatac ttatcctgac agtaatacat taataaataa atatggaaca aaagattctc gggttcttga agcaatgtgc gcagctgatg cagaaagagt agctgttcat ttgcgtcaag aagcattgcc agagaagttt gattccactt atttaaaata tcttcataaa cgcttatttg aaagcatatt cgaatgggca ggctgtactc gcaatcttac atttaaattt gaaaatggta caaccgctag catctcaaaa atgcaaatac caaattcaga tgctttcttc aaagatagta aaaaagtagg aaaaagctta cataaatttg atcaaatact ttctgacaaa aataatttgc aaaacttatc gcgcaaagaa tttgtcgagg aatcagtaaa gcttttttct tttcttaact atatacatcc cttcagagat ggcaatgggc atgcacaaca aatgtttttt gaaaaactcg cacaagctgc aggtcatcag cttgattttt cagttgtgac aaaaaaacgc atgatacatg cctgtaataa tacaatacct gcaaaaggta atgtaaatta tgaagcaatg caacatttgt tcgaagatat ctccaatcca gaaaaaacgc gccttttaaa ggactattta tgttctgcat ctgagcatag acgtaagctt ttgaatgatc aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BasaA.17330.f.B3.GE36618
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS CDYTYPDSNT LINKYGTKDS RVLEAMCAAD AERVAVHLRQ EALPEKFDST YLKYLHKRLF ESIFEWAGCT RNLTFKFENG TTASISKMQI PNSDAFFKDS KKVGKSLHKF DQILSDKNNL QNLSRKEFVE ESVKLFSFLN YIHPFRDGNG HAQQMFFEKL AQAAGHQLDF SVVTKKRMIH ACNNTIPAKG NVNYEAMQHL FEDISNPEKT RLLKDYLCSA SEHRRKLLND QIIIAPREDI DYTGIYKSSN ADSIIIETVD LHIVCCKXYF APEQLXXLKF GDKLPLNK
Validated NT Sequence
ttnnttaact ttaagaagga ganataccat ggctcaccac caccaccacc atatgtcttg cgattatact tatcctgaca gtaatacatt aataaataaa tatggaacaa aagattctcg ggttcttgaa gcaatgtgcg cagctgatgc agaaagagta gctgttcatt tgcgtcaaga agcattgcca gagaagtttg attccactta tttaaaatat cttcataaac gcttatttga aagcatattc gaatgggcag gctgtactcg caatcttaca tttaaatttg aaaatggtac aaccgctagc atctcaaaaa tgcaaatacc aaattcagat gctttcttca aagatagtaa aaaagtagga aaaagcttac ataaatttga tcaaatactt tctgacaaaa ataatttgca aaacttatcg cgcaaagaat ttgtcgagga atcagtaaag cttttttctt ttcttaacta tatacatccc ttcagagatg gcaatgggca tgcacaacaa atgttttttg aaaaactcgc acaagctgca ggtcatcagc ttgatttttc agttgtgaca aaaaaacgca tgatacatgc ctgtaataat acaatacctg caaaaggtaa tgtaaattat gaagcaatgc aacatttgtt cgaagatatc tccaatccag aaaaaacgcg ccttttaaag gactatttat gttctgcatc tgagcataga cgtaagcttt tgaatgatca aattattata gcaccacgtg aagatataga ctatacgggt atttataaga gtagtaatgc ggattctatt ataattgaaa cagtagactt acatatcgta tgctgtaaag antattttgc accagaacaa cttaangnat tgaaatttgg tgataaatta cctttaaata agtgagtaaa naggancnnn nnnnnnnnnn nccc
Expected Protein Sequence
MAHHHHHHMS CDYTYPDSNT LINKYGTKDS RVLEAMCAAD AERVAVHLRQ EALPEKFDST YLKYLHKRLF ESIFEWAGCT RNLTFKFENG TTASISKMQI PNSDAFFKDS KKVGKSLHKF DQILSDKNNL QNLSRKEFVE ESVKLFSFLN YIHPFRDGNG HAQQMFFEKL AQAAGHQLDF SVVTKKRMIH ACNNTIPAKG NVNYEAMQHL FEDISNPEKT RLLKDYLCSA SEHRRKLLND QIIIAPREDI DYTGIYKSSN ADSIIIETVD LHIVCCKDYF APEQLKALKF GDKITFK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt gcgattatac ttatcctgac agtaatacat taataaataa atatggaaca aaagattctc gggttcttga agcaatgtgc gcagctgatg cagaaagagt agctgttcat ttgcgtcaag aagcattgcc agagaagttt gattccactt atttaaaata tcttcataaa cgcttatttg aaagcatatt cgaatgggca ggctgtactc gcaatcttac atttaaattt gaaaatggta caaccgctag catctcaaaa atgcaaatac caaattcaga tgctttcttc aaagatagta aaaaagtagg aaaaagctta cataaatttg atcaaatact ttctgacaaa aataatttgc aaaacttatc gcgcaaagaa tttgtcgagg aatcagtaaa gcttttttct tttcttaact atatacatcc cttcagagat ggcaatgggc atgcacaaca aatgtttttt gaaaaactcg cacaagctgc aggtcatcag cttgattttt cagttgtgac aaaaaaacgc atgatacatg cctgtaataa tacaatacct gcaaaaggta atgtaaatta tgaagcaatg caacatttgt tcgaagatat ctccaatcca gaaaaaacgc gccttttaaa ggactattta tgttctgcat ctgagcatag acgtaagctt ttgaatgatc aaattattat agcaccacgt gaagatatag actatacggg tatttataag agtagtaatg cggattctat tataattgaa acagtagact tacatatcgt atgctgtaaa gattattttg caccagaaca acttaaggca ttgaaatttg gtgataagat tacctttaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BasaA.17330.f.B4.GE37037
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML IPAEKLTSLT LEEMLKKVKD SAVLKEDQKE IKRLSKIVYG SSKVLDQSID MINQEQEMSE KIAQQILEHP RSISKLAGFR IWFIKSPKRI RAERKLSLLS QKIKDYGYGV GLVKNMIIRN HEVEKKRLAQ EVRLPSEA
Validated NT Sequence
atcttactca cttatgcttc actaggcaac cttacttctt gtgcaaggcg ttttttttct acttcatgat ttcgaatgat catattttta acaagtccta caccataccc gtaatcttta attttttgac taagtaatga aagctttctt tcagccctta tgcgctttgg gctctttata aaccatattc taaagccagc gagtttagaa atagatcgag gatgttctaa aatttgttgg gcaatttttt cactcatttc ttgctcttga ttaatcatat cgatactttg atctaatact tttgaacttc cataaacaat ttttgataaa cgcttgattt ctttttgatc ttctttaagc acagcactgt ccttaacctt tttaagcatt tcttctaacg tcaaagaagt gagtttttct gctgggataa gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHLI PAEKLTSLTL EEMLKKVKDS AVLKEDQKEI KRLSKIVYGS SKVLDQSIDM INQEQEMSEK IAQQILEHPR SISKLAGFRI WFIKSPKRIR AERKLSLLSQ KIKDYGYGVG LVKNMIIRNH EVEKKRLAQE VRLPSEA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcttatcc cagcagaaaa actcacttct ttgacgttag aagaaatgct taaaaaggtt aaggacagtg ctgtgcttaa agaagatcaa aaagaaatca agcgtttatc aaaaattgtt tatggaagtt caaaagtatt agatcaaagt atcgatatga ttaatcaaga gcaagaaatg agtgaaaaaa ttgcccaaca aattttagaa catcctcgat ctatttctaa actcgctggc tttagaatat ggtttataaa gagcccaaag cgcataaggg ctgaaagaaa gctttcatta cttagtcaaa aaattaaaga ttacgggtat ggtgtaggac ttgttaaaaa tatgatcatt cgaaatcatg aagtagaaaa aaaacgcctt gcacaagaag taaggttgcc tagtgaagca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BasaA.17330.f.B4.PW37424
PURIFICATION DATe: 1/3/2014
CONCENTRATION: 3.2mg/ml
OBSERVED MW: 17kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML IPAEKLTSLT LEEMLKKVKD SAVLKEDQKE IKRLSKIVYG SSKVLDQSID MINQEQEMSE KIAQQILEHP RSISKLAGFR IWFIKSPKRI RAERKLSLLS QKIKDYGYGV GLVKNMIIRN HEVEKKRLAQ EVRLPSEA
Validated NT Sequence
atcttactca cttatgcttc actaggcaac cttacttctt gtgcaaggcg ttttttttct acttcatgat ttcgaatgat catattttta acaagtccta caccataccc gtaatcttta attttttgac taagtaatga aagctttctt tcagccctta tgcgctttgg gctctttata aaccatattc taaagccagc gagtttagaa atagatcgag gatgttctaa aatttgttgg gcaatttttt cactcatttc ttgctcttga ttaatcatat cgatactttg atctaatact tttgaacttc cataaacaat ttttgataaa cgcttgattt ctttttgatc ttctttaagc acagcactgt ccttaacctt tttaagcatt tcttctaacg tcaaagaagt gagtttttct gctgggataa gcatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHLI PAEKLTSLTL EEMLKKVKDS AVLKEDQKEI KRLSKIVYGS SKVLDQSIDM INQEQEMSEK IAQQILEHPR SISKLAGFRI WFIKSPKRIR AERKLSLLSQ KIKDYGYGVG LVKNMIIRNH EVEKKRLAQE VRLPSEA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTTATCC CAGCAGAAAA ACTCACTTCT TTGACGTTAG AAGAAATGCT TAAAAAGGTT AAGGACAGTG CTGTGCTTAA AGAAGATCAA AAAGAAATCA AGCGTTTATC AAAAATTGTT TATGGAAGTT CAAAAGTATT AGATCAAAGT ATCGATATGA TTAATCAAGA GCAAGAAATG AGTGAAAAAA TTGCCCAACA AATTTTAGAA CATCCTCGAT CTATTTCTAA ACTCGCTGGC TTTAGAATAT GGTTTATAAA GAGCCCAAAG CGCATAAGGG CTGAAAGAAA GCTTTCATTA CTTAGTCAAA AAATTAAAGA TTACGGGTAT GGTGTAGGAC TTGTTAAAAA TATGATCATT CGAAATCATG AAGTAGAAAA AAAACGCCTT GCACAAGAAG TAAGGTTGCC TAGTGAAGCA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT