SSGCID: Target Page

BasaA.17330.e

Bep2 protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BasaA.17330.e
ORGANISM:	Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BasaA.17330.e.B1.GE35705	full length		1	438
BasaA.17330.e.B2.GE36615	FIC		19	249

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	E6YRX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKTERVITL KQPEISIMHS SYYYTYPDTV VLKNKYGITD LYELVQRCAH DTAKEAVNLL AEPDPERFDT SYLKYLHGRL FQDVFEWAGC TRDVPFTFSD GTTATALGMS KSDSDVKFAA GREIGESLEL FDRVLAQKNN LKGLSREEFV PEAAKLFAAI NYIHPFREGN GRTQRLFFIK LARAAGHKLD FSAMTSMRMT YASIESIRND DIAPLEHLFE DISNPKKLRI LKRCIRELRE LYAADKSPPE EKLVISAREG VLYKGICRVV DQDRVVVETA NAYVNCSSDC FTPEQLSAFE LDHKVTFKVL NTDEMTKKFT ENISMRRQQE RSKQRVQHCA KAIYGNADEL NGIMRMIRIE PTLAETFVER LMPSSAEYRN NETFGREKAG HTENFGKLAD AVKSYANVIK ASSYENLQKP QKIEKHYVQV VRKFIRSL

NT Sequence

atgaagaaaa ctgaaagggt gataacctta aaacagcctg agatatctat aatgcattcg tcttattatt atacttatcc tgatactgta gtgctgaaaa ataaatatgg aatcacagac ctttatgagt tagtgcagag atgtgcacat gatacagcaa aagaagctgt taatttgctg gcagagcctg atccagagcg atttgatact tcctatttaa agtatttgca tggtcgttta tttcaggatg tatttgaatg ggcagggtgc actcgtgatg ttccatttac attttcagat ggtacgaccg ctacggcact tggcatgtca aagagcgatt ctgatgttaa gtttgcagct ggtagagaga ttggggaaag tttagaatta ttcgatcgag tacttgctca gaaaaataat ttaaaaggct tatcacgtga ggaatttgtt cctgaagcag caaaactgtt tgctgctatc aattatatac atccttttag agaaggtaat ggacgtacac aacgattgtt ttttataaag cttgccagag ctgcggggca taaacttgat ttctctgcca tgacatcaat gcgtatgaca tatgcgagta ttgaatctat aagaaatgat gatatagctc ctttggagca tttgtttgaa gatatttcca atccaaaaaa gctgcgaatt ttaaagcgct gcataagaga gctgcgagag ctgtatgcag ctgataagtc cccgccggag gagaagcttg ttatatcagc aagagaaggc gtgctctata aggggatctg tagggtcgtt gatcaagatc gcgttgtggt agaaacggca aatgcttatg taaactgcag tagcgattgt tttacaccag aacaattaag cgcattcgag cttgatcata aggtcacttt taaagttcta aatactgatg aaatgactaa aaagtttaca gagaatatct ctatgcgaag gcaacaagaa agaagcaaac aaagagtcca acattgtgca aaagctattt atggaaatgc ggatgaattg aatggaataa tgcgcatgat tcgtatagag ccaactttag cagaaacgtt tgttgaacgg cttatgcctt cttcagctga gtatcgtaat aatgagacat tcggacgcga aaaagctggg catactgaga attttggtaa acttgctgat gcagttaaaa gttatgcaaa tgttataaaa gcttctagtt atgaaaacct tcaaaagcct caaaaaatag aaaaacatta tgtgcaagta gtaagaaagt tcatcaggtc gctgtaa

Details for BasaA.17330.e.B1.GE35705

HARVESTED ON:	data unavailable
SEQUENCED ON:	7/30/2012
EXPECTED MW:	51kDa
OBSERVED MW:	78kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMK KTERVITLKQ PEISIMHSSY YYTYPDTVVL KNKYGITDLY ELVQRCAHDT AKEAVNLLAE PDPERFDTSY LKYLHGRLFQ DVFEWAGCTR DVPFTFSDGT TATALGMSKS DSDVKFAAGR EIGESLELFD RVLAQKNNLK GLSREEFVPE AAKLFAAINY IHPFREGNGR TQRLFFIKLA RAAGHKLDFS AMTSMRMTYA SIESIRNDDI APLEHLFEDI SNPKKLRILK RCIRELRELY AADKSPPEEK LVISAREGVL YKGICRVVDQ DRVVVETANA YVNCSSDCFT PEQLSAFELD HKVTFKVLNT DEMTKKFTEN ISMRRQQERS KQRVQHCAKA IYGNADELNG IMRMIRIEPT LAETFVERLM PSSAEYRNNE TFGREKAGHT ENFGKLADAV KSYANVIKAS SYENLQKPQK IEKHYVQVVR KFIRSL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga aaactgaaag ggtgataacc ttaaaacagc ctgagatatc tataatgcat tcgtcttatt attatactta tcctgatact gtagtgctga aaaataaata tggaatcaca gacctttatg agttagtgca gagatgtgca catgatacag caaaagaagc tgttaatttg ctggcagagc ctgatccaga gcgatttgat acttcctatt taaagtattt gcatggtcgt ttatttcagg atgtatttga atgggcaggg tgcactcgtg atgttccatt tacattttca gatggtacga ccgctacggc acttggcatg tcaaagagcg attctgatgt taagtttgca gctggtagag agattgggga aagtttagaa ttattcgatc gagtacttgc tcagaaaaat aatttaaaag gcttatcacg tgaggaattt gttcctgaag cagcaaaact gtttgctgct atcaattata tacatccttt tagagaaggt aatggacgta cacaacgatt gttttttata aagcttgcca gagctgcggg gcataaactt gatttctctg ccatgacatc aatgcgtatg acatatgcga gtattgaatc tataagaaat gatgatatag ctcctttgga gcatttgttt gaagatattt ccaatccaaa aaagctgcga attttaaagc gctgcataag agagctgcga gagctgtatg cagctgataa gtccccgccg gaggagaagc ttgttatatc agcaagagaa ggcgtgctct ataaggggat ctgtagggtc gttgatcaag atcgcgttgt ggtagaaacg gcaaatgctt atgtaaactg cagtagcgat tgttttacac cagaacaatt aagcgcattc gagcttgatc ataaggtcac ttttaaagtt ctaaatactg atgaaatgac taaaaagttt acagagaata tctctatgcg aaggcaacaa gaaagaagca aacaaagagt ccaacattgt gcaaaagcta tttatggaaa tgcggatgaa ttgaatggaa taatgcgcat gattcgtata gagccaactt tagcagaaac gtttgttgaa cggcttatgc cttcttcagc tgagtatcgt aataatgaga cattcggacg cgaaaaagct gggcatactg agaattttgg taaacttgct gatgcagtta aaagttatgc aaatgttata aaagcttcta gttatgaaaa ccttcaaaag cctcaaaaaa tagaaaaaca ttatgtgcaa gtagtaagaa agttcatcag gtcgctgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BasaA.17330.e.B2.GE36615

HARVESTED ON:	2/8/2013
SEQUENCED ON:	2/14/2013
EXPECTED MW:	28kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMH SSYYYTYPDT VVLKNKYGIT DLYELVQRCA HDTAKEAVNL LAEPDPERFD TSYLKYLHGR LFQDVFEWAG CTRDVPFTFS DGTTATALGM SKSDSDVKFA AGREIGESLE LFDRVLAQKN NLKGLSREEF VPEAAKLFAA INYIHPFREG NGRTQRLFFI KLARAAGHKL DFSAMTSMRM TYASIESIRN DDIAPLEHLF EDISNPKKLR ILKRCIRELR ELYAADKSPP

Validated NT Sequence

cncnnnnnnn nnttntnnnn tttaagaagg aganatacca tggctcacca ccaccaccac catatgcatt cgtcttatta ttatacttat cctgatactg tagtgctgaa aaataaatat ggaatcacag acctttatga gttagtgcag agatgtgcac atgatacagc aaaagaagct gttaatttgc tggcagagcc tgatccagag cgatttgata cttcctattt aaagtatttg catggtcgtt tatttcagga tgtatttgaa tgggcagggt gcactcgtga tgttccattt acattttcag atggtacgac cgctacggca cttggcatgt caaagagcga ttctgatgtt aagtttgcag ctggtagaga gattggggaa agtttagaat tattcgatcg agtacttgct cagaaaaata atttaaaagg cttatcacgt gaggaatttg ttcctgaagc agcaaaactg tttgctgcta tcaattatat acatcctttt agagaaggta atggacgtac acaacgattg ttttttataa agcttgccag agctgcgggg cataaacttg atttctctgc catgacatca atgcgtatga catatgcgag tattgaatct ataagaaatg atgatatagc tcctttggag catttgtttg aagatatttc caatccaaaa aagctgcgaa ttttaaagcg ctgcataaga gagctgcgag agctgtatgc agctgataag tccccgccgt aagtgagtaa gataggancc gnnnnnnnnn nnanannnan nnnnnnnnnn nnnnnnnnnn nntncnnncg cnnnnnnncn tttngnttct nnnnnnnnnn nnntnntctg ccnnnnnnng gngngnncna nnnnnnnngn ntnnnnnnnn nnnnnnnnnn nnngtnnnta cnnnnnncng nnncnnntcn nngannacnc nnnncnnnnn nttnnnannn ct

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatt cgtcttatta ttatacttat cctgatactg tagtgctgaa aaataaatat ggaatcacag acctttatga gttagtgcag agatgtgcac atgatacagc aaaagaagct gttaatttgc tggcagagcc tgatccagag cgatttgata cttcctattt aaagtatttg catggtcgtt tatttcagga tgtatttgaa tgggcagggt gcactcgtga tgttccattt acattttcag atggtacgac cgctacggca cttggcatgt caaagagcga ttctgatgtt aagtttgcag ctggtagaga gattggggaa agtttagaat tattcgatcg agtacttgct cagaaaaata atttaaaagg cttatcacgt gaggaatttg ttcctgaagc agcaaaactg tttgctgcta tcaattatat acatcctttt agagaaggta atggacgtac acaacgattg ttttttataa agcttgccag agctgcgggg cataaacttg atttctctgc catgacatca atgcgtatga catatgcgag tattgaatct ataagaaatg atgatatagc tcctttggag catttgtttg aagatatttc caatccaaaa aagctgcgaa ttttaaagcg ctgcataaga gagctgcgag agctgtatgc agctgataag tccccgccgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat