BasaA.17330.d

Bep5 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BasaA.17330.d
ORGANISM: Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BasaA.17330.d.B1.GE35704 full length 1 675
BasaA.17330.d.B2.GE36613 FIC 147 373
BasaA.17330.d.B3.GE36614 FIC and OB-Fold 147 429
BasaA.17330.d.B4.GE37036 BID 441 577
BasaA.17330.d.B5.GE37449 N-term extended FIC 1 373
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BasaA.17330.d.B5.PS01996 N-term extended FIC 1 373 unavailable

External Resources

RESOURCE REFERENCE ID
UniProt: E6YQT0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MINNIYQSLL KLKKHIQKKS TRNFISFLLK EEQKFNEMVD IDNGDGVETT HIEIFRKAPE ELLRMIDARI NMYMVYGLDI SPEKFATIRK YAANDFLRRY SSSKSDDFTL RDFFLRDLFL RDLSFIDSSL EKSKIPMDDV LNGYAYLNAY TYSNSLTIKN KYELRGIDFQ ERCAHDVIQA SVNLKEEPLP EKFDSSYLKY LHKRLFENTF EWAGYTRELP FTFSDGTVKL DTTITIPDLG CSIGSDKITN GLKEIDRILA EKNSLKDLSR EEFVRKASTI FSHFYSIYPF CSGNGCVQRI FFEKMAEAAG HRLDFSVITK KRWQYARYAA LLPTRGDKAI IHDLFEDISN PEKVNILKEF IRYENMSIDI KDKFVVAAKA GFPYTGLCKG YSGDAVMLMI DDQYVLCKKD DIAPGTLKTL KLGDRITLTV PRSKAIAEDV LIPEARIAPL TEKEIISRIE KYGSIQGITR KIRALSKCVY GSSKMLDNDI NLILVDSGLG EQHIRQHVSL LRYQLVGVTV LGVDNPARKQ ARIDYFKLEQ ALDDYISAVI YTRQIVLKGH KEQQERGERM VKMPSQAVQN IFSLSEEDQI KALNGNPSLH EEIKDLVNKV KDRLTSWERQ VLKEECCVQL ADSIGISLSR AREVTAIVKN AHKVCQLTTN REHEPKAMNY NLIEL
NT Sequence
atgattaata atatttatca aagcttattg aaattaaaga aacacataca aaagaaatca actcgtaatt ttatatcttt tttgttaaag gaagaacaaa agttcaatga aatggttgat atagacaatg gggacggtgt agaaacaacg catatagaaa tttttcgaaa agcaccagaa gagcttctta gaatgattga tgcaagaatc aatatgtata tggtttatgg gcttgatatc tcccctgaaa aattcgcaac tataagaaaa tatgctgcta atgatttttt acgcagatat tcttcatcta aatctgatga ttttacactt agggattttt tcttgagaga tttattcctt agggatttgt cttttataga ttcttcactg gagaaatcta agattccaat ggatgatgtt ttaaatggtt atgcctattt aaatgcttat acctattcta atagtttaac gataaaaaat aaatatgaat tgcgtggtat agattttcag gaaagatgtg cgcatgatgt aatacaagcg agtgttaatc tgaaagaaga gccactgcca gaaaaatttg attcttctta tctaaaatat cttcataagc gcttgtttga gaatacattt gaatgggctg gttatacccg ggagcttcca tttacatttt cggatggtac agttaagcta gatacaacaa taacaatacc cgatctgggt tgctctatag gcagtgataa aattacaaac ggtttaaagg aaattgaccg aatacttgcc gaaaaaaata gtttgaaaga tttatcacgt gaagaatttg ttcgtaaagc atcaacaata ttttctcact tttatagtat atatcctttc tgtagtggaa atggatgtgt acaacgtata ttttttgaaa aaatggcaga ggccgcaggg catagacttg acttctcagt tataacaaag aaacgttggc aatatgctcg ctatgccgca ttattaccta ctagaggtga taaagctatt atacacgatc tttttgaaga tatctctaat ccagaaaaag taaatatttt aaaggaattc atacgatatg aaaatatgtc catagatatc aaagataagt ttgttgtagc agcaaaagca ggttttcctt atacgggtct ctgtaaaggt tatagcggag atgctgttat gcttatgata gatgatcagt atgttttatg caaaaaagat gatattgcac caggaacact aaagacattg aagcttggtg ataggattac tcttacagtt ccaaggtcca aagcaattgc tgaagatgtt cttatcccag aagcaagaat agcccctttg acggaaaaag aaattatttc aagaattgaa aaatatggct ccatccaagg gattacacga aaaatcagag ctttatcaaa atgtgtttat ggaagctcga aaatgttaga taatgatata aatctgatac ttgtagattc aggtctcggt gaacaacata tcaggcagca tgtgagtctt ttaagatatc agcttgtagg tgtgacagta ttaggtgtcg acaatccagc gcgcaaacaa gctaggatag attactttaa gctcgagcaa gcattggatg attatataag tgctgtaatt tatactagac aaatagtact gaaagggcac aaagaacaac aagaacgtgg cgagcgaatg gtaaaaatgc ctagtcaagc agtgcaaaat attttttctt tatctgaaga agatcagatc aaagctttaa atggcaatcc ttcccttcac gaagagatta aagatcttgt gaataaagtc aaagatcgcc ttacatcatg ggagcgtcaa gtacttaagg aagaatgttg tgttcagttg gctgatagta taggcatatc actaagtaga gcgcgggaag ttacagcaat tgttaagaac gctcataaag tatgtcagct aacaacaaac agagagcatg agccgaaagc gatgaactat aacctgatag aactctaa
Details for BasaA.17330.d.B1.GE35704
HARVESTED ON: 7/10/2012
SEQUENCED ON: 7/30/2012
EXPECTED MW: 78kDa
OBSERVED MW: 78kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 78
PERCENT COVERAGE: 58
Validated AA Sequence
MAHHHHHHMI NNIYQSLLKL KKHIQKKSTR NFISFLLKEE QKFNEMVDID NGDGVETTHI EIFRKAPEEL LRMIDARINM YMVYGLDISP EKFATIRKYA ANDFLRRYSS SKSDDFTLRD FFLRDLFLRD LSFIDSSLEK SKIPMDDVLN GYAYLNAYTY SNSLTIKNKY ELRGIDFQER CAHDVIQASV NLKEEPLPEK FDSSYLKYLH KRLFENTFEW AGYTRELPFT FSDGTVKLDT TITIPDLGCS IGSDKITNGL KEIDRILAEK NSLKXLSREE FVRKASTIFS HFYSIYPFXS GNGCVQRIFF EKWXXXXXXL DXSXXXEXLX XXRYXXXXLX XXXXYXIXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
ttgtttaact ttaagaagga ganataccat ggctcaccac caccaccacc atatgattaa taatatttat caaagcttat tgaaattaaa gaaacacata caaaagaaat caactcgtaa ttttatatct tttttgttaa aggaagaaca aaagttcaat gaaatggttg atatagacaa tggggacggt gtagaaacaa cgcatataga aatttttcga aaagcaccag aagagcttct tagaatgatt gatgcaagaa tcaatatgta tatggtttat gggcttgata tctcccctga aaaattcgca actataagaa aatatgctgc taatgatttt ttacgcagat attcttcatc taaatctgat gattttacac ttagggattt tttcttgaga gatttattcc ttagggattt gtcttttata gattcttcac tggagaaatc taagattcca atggatgatg ttttaaatgg ttatgcctat ttaaatgctt atacctattc taatagttta acgataaaaa ataaatatga attgcgtggt atagattttc aggaaagatg tgcgcatgat gtaatacaag cgagtgttaa tctgaaagaa gagccactgc cagaaaaatt tgattcttct tatctaaaat atcttcataa gcgcttgttt gagaatacat ttgaatgggc tggttatacc cgggagcttc catttacatt ttcggatggt acagttaagc tagatacaac aataacaata cccgatctgg gttgctctat aggcagtgat aaaattacaa acggtttaaa ggaaattgac cgaatacttg ccgaaaaaaa tagtttgaaa ganttatcac gtgaagaatt tgttcgtaaa gcatcaacaa tattttctca cttttatagt atatatcctt tctgnagtgg aaatggntgt gtacaacgta tattttttga aaaatggnnn nnnnncnggn atanacttga ctnctcantn nnnnaagaan cgttggnana tgntcgctat ncncnnnnnc nactanagng anaanctntn tacncnatcn ttttnannnn ncnancnnaa aannannnnt tnnnnnnnnn nnnnntnaan nnnnncnnnn nnnnnnnnnn nnannnnnnn nnnnnnnncn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tnnnnnnnaa nnnnnnatnn nnnnnnnnnn cnnnnna
Expected Protein Sequence
MAHHHHHHMI NNIYQSLLKL KKHIQKKSTR NFISFLLKEE QKFNEMVDID NGDGVETTHI EIFRKAPEEL LRMIDARINM YMVYGLDISP EKFATIRKYA ANDFLRRYSS SKSDDFTLRD FFLRDLFLRD LSFIDSSLEK SKIPMDDVLN GYAYLNAYTY SNSLTIKNKY ELRGIDFQER CAHDVIQASV NLKEEPLPEK FDSSYLKYLH KRLFENTFEW AGYTRELPFT FSDGTVKLDT TITIPDLGCS IGSDKITNGL KEIDRILAEK NSLKDLSREE FVRKASTIFS HFYSIYPFCS GNGCVQRIFF EKMAEAAGHR LDFSVITKKR WQYARYAALL PTRGDKAIIH DLFEDISNPE KVNILKEFIR YENMSIDIKD KFVVAAKAGF PYTGLCKGYS GDAVMLMIDD QYVLCKKDDI APGTLKTLKL GDRITLTVPR SKAIAEDVLI PEARIAPLTE KEIISRIEKY GSIQGITRKI RALSKCVYGS SKMLDNDINL ILVDSGLGEQ HIRQHVSLLR YQLVGVTVLG VDNPARKQAR IDYFKLEQAL DDYISAVIYT RQIVLKGHKE QQERGERMVK MPSQAVQNIF SLSEEDQIKA LNGNPSLHEE IKDLVNKVKD RLTSWERQVL KEECCVQLAD SIGISLSRAR EVTAIVKNAH KVCQLTTNRE HEPKAMNYNL IEL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatta ataatattta tcaaagctta ttgaaattaa agaaacacat acaaaagaaa tcaactcgta attttatatc ttttttgtta aaggaagaac aaaagttcaa tgaaatggtt gatatagaca atggggacgg tgtagaaaca acgcatatag aaatttttcg aaaagcacca gaagagcttc ttagaatgat tgatgcaaga atcaatatgt atatggttta tgggcttgat atctcccctg aaaaattcgc aactataaga aaatatgctg ctaatgattt tttacgcaga tattcttcat ctaaatctga tgattttaca cttagggatt ttttcttgag agatttattc cttagggatt tgtcttttat agattcttca ctggagaaat ctaagattcc aatggatgat gttttaaatg gttatgccta tttaaatgct tatacctatt ctaatagttt aacgataaaa aataaatatg aattgcgtgg tatagatttt caggaaagat gtgcgcatga tgtaatacaa gcgagtgtta atctgaaaga agagccactg ccagaaaaat ttgattcttc ttatctaaaa tatcttcata agcgcttgtt tgagaataca tttgaatggg ctggttatac ccgggagctt ccatttacat tttcggatgg tacagttaag ctagatacaa caataacaat acccgatctg ggttgctcta taggcagtga taaaattaca aacggtttaa aggaaattga ccgaatactt gccgaaaaaa atagtttgaa agatttatca cgtgaagaat ttgttcgtaa agcatcaaca atattttctc acttttatag tatatatcct ttctgtagtg gaaatggatg tgtacaacgt atattttttg aaaaaatggc agaggccgca gggcatagac ttgacttctc agttataaca aagaaacgtt ggcaatatgc tcgctatgcc gcattattac ctactagagg tgataaagct attatacacg atctttttga agatatctct aatccagaaa aagtaaatat tttaaaggaa ttcatacgat atgaaaatat gtccatagat atcaaagata agtttgttgt agcagcaaaa gcaggttttc cttatacggg tctctgtaaa ggttatagcg gagatgctgt tatgcttatg atagatgatc agtatgtttt atgcaaaaaa gatgatattg caccaggaac actaaagaca ttgaagcttg gtgataggat tactcttaca gttccaaggt ccaaagcaat tgctgaagat gttcttatcc cagaagcaag aatagcccct ttgacggaaa aagaaattat ttcaagaatt gaaaaatatg gctccatcca agggattaca cgaaaaatca gagctttatc aaaatgtgtt tatggaagct cgaaaatgtt agataatgat ataaatctga tacttgtaga ttcaggtctc ggtgaacaac atatcaggca gcatgtgagt cttttaagat atcagcttgt aggtgtgaca gtattaggtg tcgacaatcc agcgcgcaaa caagctagga tagattactt taagctcgag caagcattgg atgattatat aagtgctgta atttatacta gacaaatagt actgaaaggg cacaaagaac aacaagaacg tggcgagcga atggtaaaaa tgcctagtca agcagtgcaa aatatttttt ctttatctga agaagatcag atcaaagctt taaatggcaa tccttccctt cacgaagaga ttaaagatct tgtgaataaa gtcaaagatc gccttacatc atgggagcgt caagtactta aggaagaatg ttgtgttcag ttggctgata gtataggcat atcactaagt agagcgcggg aagttacagc aattgttaag aacgctcata aagtatgtca gctaacaaca aacagagagc atgagccgaa agcgatgaac tataacctga tagaactctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BasaA.17330.d.B2.GE36613
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 27kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML NAYTYSNSLT IKNKYELRGI DFQERCAHDV IQASVNLKEE PLPEKFDSSY LKYLHKRLFE NTFEWAGYTR ELPFTFSDGT VKLDTTITIP DLGCSIGSDK ITNGLKEIDR ILAEKNSLKD LSREEFVRKA STIFSHFYSI YPFCSGNGCV QRIFFEKMAE AAGHRLDFSV ITKKRWQYAR YAALLPTRGD KAIIHDLFED ISNPEKVNIL KEFIRYENMS IDIKDK
Validated NT Sequence
tttntnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgttaaa tgcttatacc tattctaata gtttaacgat aaaaaataaa tatgaattgc gtggtataga ttttcaggaa agatgtgcgc atgatgtaat acaagcgagt gttaatctga aagaagagcc actgccagaa aaatttgatt cttcttatct aaaatatctt cataagcgct tgtttgagaa tacatttgaa tgggctggtt atacccggga gcttccattt acattttcgg atggtacagt taagctagat acaacaataa caatacccga tctgggttgc tctataggca gtgataaaat tacaaacggt ttaaaggaaa ttgaccgaat acttgccgaa aaaaatagtt tgaaagattt atcacgtgaa gaatttgttc gtaaagcatc aacaatattt tctcactttt atagtatata tcctttctgt agtggaaatg gatgtgtaca acgtatattt tttgaaaaaa tggcagaggc cgcagggcat agacttgact tctcagttat aacaaagaaa cgttggcaat atgctcgcta tgccgcatta ttacctacta gaggtgataa agctattata cacgatcttt ttgaagatat ctctaatcca gaaaaagtaa atattttaaa ggaattcata cgatatgaaa atatgtccat agatatcaaa gataagtaag tgagtaagat aggatccgnn nnnnnnnnnn ncnnnnnnna annnnnnnnn nnnnnnaaaa aannnnnnag aannnnnnnc ncgnnnncnn cncnntnnnn ntnnnnnnnn ntnnttnnnn ntgntgcnnn nntcagnnnn nananngann nnnnnnnnnn nnnnnannna nncnnnnntc cnttnnnccg nnnnnnnncn nnnttnnnnn cnnnncccnt
Expected Protein Sequence
MAHHHHHHML NAYTYSNSLT IKNKYELRGI DFQERCAHDV IQASVNLKEE PLPEKFDSSY LKYLHKRLFE NTFEWAGYTR ELPFTFSDGT VKLDTTITIP DLGCSIGSDK ITNGLKEIDR ILAEKNSLKD LSREEFVRKA STIFSHFYSI YPFCSGNGCV QRIFFEKMAE AAGHRLDFSV ITKKRWQYAR YAALLPTRGD KAIIHDLFED ISNPEKVNIL KEFIRYENMS IDIKDK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa atgcttatac ctattctaat agtttaacga taaaaaataa atatgaattg cgtggtatag attttcagga aagatgtgcg catgatgtaa tacaagcgag tgttaatctg aaagaagagc cactgccaga aaaatttgat tcttcttatc taaaatatct tcataagcgc ttgtttgaga atacatttga atgggctggt tatacccggg agcttccatt tacattttcg gatggtacag ttaagctaga tacaacaata acaatacccg atctgggttg ctctataggc agtgataaaa ttacaaacgg tttaaaggaa attgaccgaa tacttgccga aaaaaatagt ttgaaagatt tatcacgtga agaatttgtt cgtaaagcat caacaatatt ttctcacttt tatagtatat atcctttctg tagtggaaat ggatgtgtac aacgtatatt ttttgaaaaa atggcagagg ccgcagggca tagacttgac ttctcagtta taacaaagaa acgttggcaa tatgctcgct atgccgcatt attacctact agaggtgata aagctattat acacgatctt tttgaagata tctctaatcc agaaaaagta aatattttaa aggaattcat acgatatgaa aatatgtcca tagatatcaa agataagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BasaA.17330.d.B3.GE36614
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML NAYTYSNSLT IKNKYELRGI DFQERCAHDV IQASVNLKEE PLPEKFDSSY LKYLHKRLFE NTFEWAGYTR ELPFTFSDGT VKLDTTITIP DLGCSIGSDK ITNGLKEIDR ILAEKNSLKD LSREEFVRKA STIFSHFYSI YPFCSGNGCV QRIFFEKMAE AAGHRLDFSV ITKKRWQYAR YAALLPTRGD KAIIHDLFED ISNPEKVNIL KEFIRYENMS IDIKDKFVVA AKAGFPYTGL CKGYSGDAVM LMIDDQYVLC KKDDIAPGTL KTLKLGDRIT LT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa atgcttatac ctattctaat agtttaacga taaaaaataa atatgaattg cgtggtatag attttcagga aagatgtgcg catgatgtaa tacaagcgag tgttaatctg aaagaagagc cactgccaga aaaatttgat tcttcttatc taaaatatct tcataagcgc ttgtttgaga atacatttga atgggctggt tatacccggg agcttccatt tacattttcg gatggtacag ttaagctaga tacaacaata acaatacccg atctgggttg ctctataggc agtgataaaa ttacaaacgg tttaaaggaa attgaccgaa tacttgccga aaaaaatagt ttgaaagatt tatcacgtga agaatttgtt cgtaaagcat caacaatatt ttctcacttt tatagtatat atcctttctg tagtggaaat ggatgtgtac aacgtatatt ttttgaaaaa atggcagagg ccgcagggca tagacttgac ttctcagtta taacaaagaa acgttggcaa tatgctcgct atgccgcatt attacctact agaggtgata aagctattat acacgatctt tttgaagata tctctaatcc agaaaaagta aatattttaa aggaattcat acgatatgaa aatatgtcca tagatatcaa agataagttt gttgtagcag caaaagcagg ttttccttat acgggtctct gtaaaggtta tagcggagat gctgttatgc ttatgataga tgatcagtat gttttatgca aaaaagatga tattgcacca ggaacactaa agacattgaa gcttggtgat aggattactc ttacatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BasaA.17330.d.B4.GE37036
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 17kDa
OBSERVED MW: 15kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML IPEARIAPLT EKEIISRIEK YGSIQGITRK IRALSKCVYG SSKMLDNDIN LILVDSGLGE QHIRQHVSLL RYQLVGVTVL GVDNPARKQA RIDYFKLEQA LDDYISAVIY TRQIVLKGHK EQQERGERMV KMPSQA
Validated NT Sequence
atcttactca cttatgcttg actaggcatt tttaccattc gctcgccacg ttcttgttgt tctttgtgcc ctttcagtac tatttgtcta gtataaatta cagcacttat ataatcatcc aatgcttgct cgagcttaaa gtaatctatc ctagcttgtt tgcgcgctgg attgtcgaca cctaatactg tcacacctac aagctgatat cttaaaagac tcacatgctg cctgatatgt tgttcaccga gacctgaatc tacaagtatc agatttatat cattatctaa cattttcgag cttccataaa cacattttga taaagctctg atttttcgtg taatcccttg gatggagcca tatttttcaa ttcttgaaat aatttctttt tccgtcaaag gggctattct tgcttctggg ataagcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHLI PEARIAPLTE KEIISRIEKY GSIQGITRKI RALSKCVYGS SKMLDNDINL ILVDSGLGEQ HIRQHVSLLR YQLVGVTVLG VDNPARKQAR IDYFKLEQAL DDYISAVIYT RQIVLKGHKE QQERGERMVK MPSQA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcttatcc cagaagcaag aatagcccct ttgacggaaa aagaaattat ttcaagaatt gaaaaatatg gctccatcca agggattaca cgaaaaatca gagctttatc aaaatgtgtt tatggaagct cgaaaatgtt agataatgat ataaatctga tacttgtaga ttcaggtctc ggtgaacaac atatcaggca gcatgtgagt cttttaagat atcagcttgt aggtgtgaca gtattaggtg tcgacaatcc agcgcgcaaa caagctagga tagattactt taagctcgag caagcattgg atgattatat aagtgctgta atttatacta gacaaatagt actgaaaggg cacaaagaac aacaagaacg tggcgagcga atggtaaaaa tgcctagtca agcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BasaA.17330.d.B5.GE37449
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 45kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 64
Validated AA Sequence
MINNIYQSLL KLKKHIQKKS TRNFISFLLK EEQKFNEMVD IDNGDGVETT HIEIFRKAPE ELLRMIDARI NMYMVYGLDI SPEKFATIRK YAANDFLRRY SSSKSDDFTL RDFFLRDLFL RDLSFIDSSL EKSKIPMDDV LNGYAYLNAY TYSNSLTIKN KYELRGIDFQ ERCAHDVIQA SVNLKEEPLP EKFDSSYLKY LHKRLFENTF EWAGYTRELP FTFSDGTVKL DTTITIPDLG LLYRQ
Validated NT Sequence
catatcgtat gaattccttt aaaatattta ctttttctgg attagagata tcttcaaaaa gatcgtgtat aatagcttta tcacctctag taggtaataa tgcggcatag cgagcatatt gccaacgttt ctttgttata actgagaagt caagtctatg ccctgcggcc tctgccattt tttcaaaaaa tatacgttgt acacatccat ttccactaca gaaaggatat atactataaa agtgagaaaa tattgttgat gctttacgaa caaattcttc acgtgataaa tctttcaaac tatttttttc ggcaagtatt cggtcaattt cctttaaacc gtttgtaatt ttatcactgc ctatagagca accccagatc gggtattgtt attgttgtat ctagcttaac tgtaccatcc gaaaatgtaa atggaagctc ccgggtataa ccagcccatt caaatgtatt ctcaaacaag cgcttatgaa gatattttag ataagaagaa tcaaattttt ctggcagtgg ctcttctttc agattaacac tcgcttgtat tacatcatgc gcacatcttt cctgaaaatc tataccacgc aattcatatt tattttttat cgttaaacta ttagaatagg tataagcatt taaataggca taaccattta aaacatcatc cattggaatc ttagatttct ccagtgaaga atctataaaa gacaaatccc taaggaataa atctctcaag aaaaaatccc taagtgtaaa atcatcagat ttagatgaag aatatctgcg taaaaaatca ttagcagcat attttcttat agttgcgaat ttttcagggg agatatcaag cccataaacc atatacatat tgattcttgc atcaatcatt ctaagaagct cttctggtgc ttttcgaaaa atttctatat gcgttgtttc tacaccgtcc ccattgtcta tatcaaccat ttcattgaac ttttgttctt cctttaacaa aaaagatata aaattacgag ttgatttctt ttgtatgtgt ttctttaatt tcaataagct ttgataaata ttattaatca tatggtggtg gtg
Expected Protein Sequence
MAHHHHHHMI NNIYQSLLKL KKHIQKKSTR NFISFLLKEE QKFNEMVDID NGDGVETTHI EIFRKAPEEL LRMIDARINM YMVYGLDISP EKFATIRKYA ANDFLRRYSS SKSDDFTLRD FFLRDLFLRD LSFIDSSLEK SKIPMDDVLN GYAYLNAYTY SNSLTIKNKY ELRGIDFQER CAHDVIQASV NLKEEPLPEK FDSSYLKYLH KRLFENTFEW AGYTRELPFT FSDGTVKLDT TITIPDLGCS IGSDKITNGL KEIDRILAEK NSLKDLSREE FVRKASTIFS HFYSIYPFCS GNGCVQRIFF EKMAEAAGHR LDFSVITKKR WQYARYAALL PTRGDKAIIH DLFEDISNPE KVNILKEFIR YENMSIDIKD K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatta ataatattta tcaaagctta ttgaaattaa agaaacacat acaaaagaaa tcaactcgta attttatatc ttttttgtta aaggaagaac aaaagttcaa tgaaatggtt gatatagaca atggggacgg tgtagaaaca acgcatatag aaatttttcg aaaagcacca gaagagcttc ttagaatgat tgatgcaaga atcaatatgt atatggttta tgggcttgat atctcccctg aaaaattcgc aactataaga aaatatgctg ctaatgattt tttacgcaga tattcttcat ctaaatctga tgattttaca cttagggatt ttttcttgag agatttattc cttagggatt tgtcttttat agattcttca ctggagaaat ctaagattcc aatggatgat gttttaaatg gttatgccta tttaaatgct tatacctatt ctaatagttt aacgataaaa aataaatatg aattgcgtgg tatagatttt caggaaagat gtgcgcatga tgtaatacaa gcgagtgtta atctgaaaga agagccactg ccagaaaaat ttgattcttc ttatctaaaa tatcttcata agcgcttgtt tgagaataca tttgaatggg ctggttatac ccgggagctt ccatttacat tttcggatgg tacagttaag ctagatacaa caataacaat acccgatctg ggttgctcta taggcagtga taaaattaca aacggtttaa aggaaattga ccgaatactt gccgaaaaaa atagtttgaa agatttatca cgtgaagaat ttgttcgtaa agcatcaaca atattttctc acttttatag tatatatcct ttctgtagtg gaaatggatg tgtacaacgt atattttttg aaaaaatggc agaggccgca gggcatagac ttgacttctc agttataaca aagaaacgtt ggcaatatgc tcgctatgcc gcattattac ctactagagg tgataaagct attatacacg atctttttga agatatctct aatccagaaa aagtaaatat tttaaaggaa ttcatacgat atgaaaatat gtccatagat atcaaagata agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BasaA.17330.d.B5.PS01996
PURIFICATION DATe: 2/13/2014
CONCENTRATION: 4.76mg/ml
OBSERVED MW: 44kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: 110µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 64
Protocol Notes
notes unavailable
Validated AA Sequence
MINNIYQSLL KLKKHIQKKS TRNFISFLLK EEQKFNEMVD IDNGDGVETT HIEIFRKAPE ELLRMIDARI NMYMVYGLDI SPEKFATIRK YAANDFLRRY SSSKSDDFTL RDFFLRDLFL RDLSFIDSSL EKSKIPMDDV LNGYAYLNAY TYSNSLTIKN KYELRGIDFQ ERCAHDVIQA SVNLKEEPLP EKFDSSYLKY LHKRLFENTF EWAGYTRELP FTFSDGTVKL DTTITIPDLG LLYRQ
Validated NT Sequence
catatcgtat gaattccttt aaaatattta ctttttctgg attagagata tcttcaaaaa gatcgtgtat aatagcttta tcacctctag taggtaataa tgcggcatag cgagcatatt gccaacgttt ctttgttata actgagaagt caagtctatg ccctgcggcc tctgccattt tttcaaaaaa tatacgttgt acacatccat ttccactaca gaaaggatat atactataaa agtgagaaaa tattgttgat gctttacgaa caaattcttc acgtgataaa tctttcaaac tatttttttc ggcaagtatt cggtcaattt cctttaaacc gtttgtaatt ttatcactgc ctatagagca accccagatc gggtattgtt attgttgtat ctagcttaac tgtaccatcc gaaaatgtaa atggaagctc ccgggtataa ccagcccatt caaatgtatt ctcaaacaag cgcttatgaa gatattttag ataagaagaa tcaaattttt ctggcagtgg ctcttctttc agattaacac tcgcttgtat tacatcatgc gcacatcttt cctgaaaatc tataccacgc aattcatatt tattttttat cgttaaacta ttagaatagg tataagcatt taaataggca taaccattta aaacatcatc cattggaatc ttagatttct ccagtgaaga atctataaaa gacaaatccc taaggaataa atctctcaag aaaaaatccc taagtgtaaa atcatcagat ttagatgaag aatatctgcg taaaaaatca ttagcagcat attttcttat agttgcgaat ttttcagggg agatatcaag cccataaacc atatacatat tgattcttgc atcaatcatt ctaagaagct cttctggtgc ttttcgaaaa atttctatat gcgttgtttc tacaccgtcc ccattgtcta tatcaaccat ttcattgaac ttttgttctt cctttaacaa aaaagatata aaattacgag ttgatttctt ttgtatgtgt ttctttaatt tcaataagct ttgataaata ttattaatca tatggtggtg gtg
Expressed Protein Sequence
MAHHHHHHMI NNIYQSLLKL KKHIQKKSTR NFISFLLKEE QKFNEMVDID NGDGVETTHI EIFRKAPEEL LRMIDARINM YMVYGLDISP EKFATIRKYA ANDFLRRYSS SKSDDFTLRD FFLRDLFLRD LSFIDSSLEK SKIPMDDVLN GYAYLNAYTY SNSLTIKNKY ELRGIDFQER CAHDVIQASV NLKEEPLPEK FDSSYLKYLH KRLFENTFEW AGYTRELPFT FSDGTVKLDT TITIPDLGCS IGSDKITNGL KEIDRILAEK NSLKDLSREE FVRKASTIFS HFYSIYPFCS GNGCVQRIFF EKMAEAAGHR LDFSVITKKR WQYARYAALL PTRGDKAIIH DLFEDISNPE KVNILKEFIR YENMSIDIKD K
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTA ATAATATTTA TCAAAGCTTA TTGAAATTAA AGAAACACAT ACAAAAGAAA TCAACTCGTA ATTTTATATC TTTTTTGTTA AAGGAAGAAC AAAAGTTCAA TGAAATGGTT GATATAGACA ATGGGGACGG TGTAGAAACA ACGCATATAG AAATTTTTCG AAAAGCACCA GAAGAGCTTC TTAGAATGAT TGATGCAAGA ATCAATATGT ATATGGTTTA TGGGCTTGAT ATCTCCCCTG AAAAATTCGC AACTATAAGA AAATATGCTG CTAATGATTT TTTACGCAGA TATTCTTCAT CTAAATCTGA TGATTTTACA CTTAGGGATT TTTTCTTGAG AGATTTATTC CTTAGGGATT TGTCTTTTAT AGATTCTTCA CTGGAGAAAT CTAAGATTCC AATGGATGAT GTTTTAAATG GTTATGCCTA TTTAAATGCT TATACCTATT CTAATAGTTT AACGATAAAA AATAAATATG AATTGCGTGG TATAGATTTT CAGGAAAGAT GTGCGCATGA TGTAATACAA GCGAGTGTTA ATCTGAAAGA AGAGCCACTG CCAGAAAAAT TTGATTCTTC TTATCTAAAA TATCTTCATA AGCGCTTGTT TGAGAATACA TTTGAATGGG CTGGTTATAC CCGGGAGCTT CCATTTACAT TTTCGGATGG TACAGTTAAG CTAGATACAA CAATAACAAT ACCCGATCTG GGTTGCTCTA TAGGCAGTGA TAAAATTACA AACGGTTTAA AGGAAATTGA CCGAATACTT GCCGAAAAAA ATAGTTTGAA AGATTTATCA CGTGAAGAAT TTGTTCGTAA AGCATCAACA ATATTTTCTC ACTTTTATAG TATATATCCT TTCTGTAGTG GAAATGGATG TGTACAACGT ATATTTTTTG AAAAAATGGC AGAGGCCGCA GGGCATAGAC TTGACTTCTC AGTTATAACA AAGAAACGTT GGCAATATGC TCGCTATGCC GCATTATTAC CTACTAGAGG TGATAAAGCT ATTATACACG ATCTTTTTGA AGATATCTCT AATCCAGAAA AAGTAAATAT TTTAAAGGAA TTCATACGAT ATGAAAATAT GTCCATAGAT ATCAAAGATA AGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT