BasaA.17330.c
Bep8 protein (VirB-translocated Bartonella effector protein)
| CENTER ID: | BasaA.17330.c |
| ORGANISM: | Bartonella sp. AR 15-3 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BasaA.17330.c.B1.GE35664 | full length | 1 | 541 | ||
| BasaA.17330.c.B2.GE36611 | FIC | 9 | 240 | ||
| BasaA.17330.c.B3.GE36612 | FIC and OB-Fold | 9 | 296 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BasaA.17330.c.B3.PS01856 | FIC and OB-Fold | 9 | 296 | ||
| BasaA.17330.c.B2.PW37292 | FIC | 9 | 240 |
Structures
4M16
DEPOSITED: 8/2/2013
DETERMINATION: XRay
CLONE: BasaA.17330.c.B2.GE36611
PROTEIN: BasaA.17330.c.B2.PW37292
Publications by SSGCID
Evolutionary Diversification of Host-Targeted Bartonella Effectors Proteins Derived from a Conserved FicTA Toxin-Antitoxin Module
de Beer T, Dehio C, Edwards T, Harms A, Myler P, Phan I, Schirmer T, Dranow D, Tamegger S, Dietz N
Microorganisms - 2021
volume 9, issue 8, pages 1645
PMID: 34442725; PMCID: PMC8401265
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_171090 |
| UniProt: | E6YQQ1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGVLITSPVL NYFYPGTKTL KNKLGIMGYK QLEKRCKRNA KKVINSLRNE PLPETFDSSY LKYLHKRLFG SAFEWAGYTR DLSFAFDDGT IAQMSMMKIP GTDIYFAHGD KIQENLKEFD EILASKSNLQ GLSREDFIEE TVKLFSFLNY IHPFRAGNEA VQHIFFEKLA EAAGHKLDFS VVTEERIMRA CNDAMALKGE EAHQAMKSLF EDISNPEEVI ILRDFFKHIP RVERQRLNDE YVTMPREGTT YTGIYQLGSP NSLLFKTVDS HILCLKDYFT PEQLKALKSG GKITFTVPMK KDLDPILIPG EKLAPLKEEE IIKRTRRADC IRDHQREIDR YSKLIYGDST ILDKHMMRIH EYPETGMQLA EQIRTSPQSI AKLAGFKIWF IKSPKRRMAE NSILGLTEKI EKHAQIVRSV RDKIFMEHQK EQKRIECVVK KPSKKIQDIL NLPKDEQKKV LESSPTLYKD FCSFLDEVNS RLTVDQQNYI FDKKEIEFSD SVGMSRKKAR MVMNIIDKSV RLHKQITAIK PDCSQVMAMT I
NT Sequence
atgggagtac tcattacatc tccagtgctg aattattttt atcctggtac taagacgtta aagaataaat tgggtataat gggctataaa caacttgaaa aaagatgcaa gcgtaatgcg aaaaaagtga tcaatagtct gcgtaacgaa ccactaccag aaacatttga ttcttcctat ctaaaatatc ttcataaacg cctgttcggg agtgcattcg aatgggctgg atatacccgt gatctttcat ttgcatttga tgatggtact attgctcaaa tgtcaatgat gaaaattccg ggaacagata tttattttgc acatggtgat aaaattcaag aaaacttaaa ggaatttgat gaaatacttg cttcgaagag taatttgcaa ggtttatcgc gtgaagattt tattgaggaa acagtaaagc ttttttcttt tctcaactat atacatcctt ttagagctgg taatgaagct gtacagcaca tattttttga aaaacttgct gaagctgcgg gccataagct tgatttttcg gttgtgacag aagagcgtat aatgcgtgcc tgtaatgatg caatggctct aaagggcgag gaagcccatc aagcaatgaa gtctttgttt gaagatatct ctaatccaga agaagtaatt attttaagag atttctttaa acacatacct agagttgagc gccaacgttt gaatgatgaa tatgttacaa tgccgcggga aggtacaact tatacaggta tttatcaact aggtagtcca aattctcttc tgtttaaaac ggtggattcg catattctat gtctgaaaga ttattttacg ccagaacaac tcaaggcatt aaaatctggt ggtaagatta catttacagt tccaatgaaa aaagacttag atccaattct cattccagga gaaaaattag ctcctttgaa ggaagaagaa ataatcaaac ggactagaag ggctgattgt attagagacc accaaagaga aattgatcgt tactcaaagc tcatttatgg agacagcacg atattagata aacatatgat gcgtattcat gaatatcctg aaacgggtat gcagcttgcc gaacagatta gaacatctcc tcaatctatt gctaagctcg cgggctttaa aatatggttt ataaagagtc caaaacgtag aatggctgag aatagtattt tgggactcac tgaaaaaatt gaaaaacatg cgcaaattgt gcgatctgtt agggataaaa tttttatgga gcatcaaaaa gaacaaaaac gtattgaatg cgtagtaaaa aaacccagca aaaaaataca agatattctt aatttgccaa aagatgaaca aaaaaaggtc ttagaatcct ctcctacact ttataaagat ttctgtagtt ttctagatga agtaaattct cgtcttacag tagatcagca aaattacatc tttgataaaa aggagataga attttctgac agtgttggca tgtcaagaaa gaaagcaagg atggttatga acataattga taaaagtgta agattacaca agcaaattac agcaattaaa cctgattgtt cgcaagtaat ggctatgact atttga
Details for BasaA.17330.c.B1.GE35664
| HARVESTED ON: | 7/10/2012 |
| SEQUENCED ON: | 2/22/2016 |
| EXPECTED MW: | 63kDa |
| OBSERVED MW: | 63kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG VLITSPVLNY FYPGTKTLKN KLGIMGYKQL EKRCKRNAKK VINSLRNEPL PETFDSSYLK YLHKRLFGSA FEWAGYTRDL SFAFDDGTIA QMSMMKIPGT DIYFAHGDKI QENLKEFDEI LASKSNLQGL SREDFIEETV KLFSFLNYIH PFRAGNEAVQ HIFFEKLAEA AGHKLDFSVV TEERIMRACN DAMALKGEEA HQAMKSLFED ISNPEEVIIL RDFFKHIPRV ERQRLNDEYV TMPREGTTYT GIYQLGSPNS LLFKTVDSHI LCLKDYFTPE QLKALKSGGK ITFTVPMKKD LDPILIPGEK LAPLKEEEII KRTRRADCIR DHQREIDRYS KLIYGDSTIL DKHMMRIHEY PETGMQLAEQ IRTSPQSIAK LAGFKIWFIK SPKRRMAENS ILGLTEKIEK HAQIVRSVRD KIFMEHQKEQ KRIECVVKKP SKKIQDILNL PKDEQKKVLE SSPTLYKDFC SFLDEVNSRL TVDQQNYIFD KKEIEFSDSV GMSRKKARMV MNIIDKSVRL HKQITAIKPD CSQVMAMTI
Validated NT Sequence
atggctcacc accaccacca ccatatggga gtactcatta catctccagt gctgaattat ttttatcctg gtactaagac gttaaagaat aaattgggta taatgggcta taaacaactt gaaaaaagat gcaagcgtaa tgcgaaaaaa gtgatcaata gtctgcgtaa cgaaccacta ccagaaacat ttgattcttc ctatctaaaa tatcttcata aacgcctgtt cgggagtgca ttcgaatggg ctggatatac ccgtgatctt tcatttgcat ttgatgatgg tactattgct caaatgtcaa tgatgaaaat tccgggaaca gatatttatt ttgcacatgg tgataaaatt caagaaaact taaaggaatt tgatgaaata cttgcttcga agagtaattt gcaaggttta tcgcgtgaag attttattga ggaaacagta aagctttttt cttttctcaa ctatatacat ccttttagag ctggtaatga agctgtacag cacatatttt ttgaaaaact tgctgaagct gcgggccata agcttgattt ttcggttgtg acagaagagc gtataatgcg tgcctgtaat gatgcaatgg ctctaaaggg cgaggaagcc catcaagcaa tgaagtcttt gtttgaagat atctctaatc cagaagaagt aattatttta agagatttct ttaaacacat acctagagtt gagcgccaac gtttgaatga tgaatatgtt acaatgccgc gggaaggtac aacttataca ggtatttatc aactaggtag tccaaattct cttctgttta aaacggtgga ttcgcatatt ctatgtctga aagattattt tacgccagaa caactcaagg cattaaaatc tggtggtaag attacattta cagttccaat gaaaaaagac ttagatccaa ttctcattcc aggagaaaaa ttagctcctt tgaaggaaga agaaataatc aaacggacta gaagggctga ttgtattaga gaccaccaaa gagaaattga tcgttactca aagctcattt atggagacag cacgatatta gataaacata tgatgcgtat tcatgaatat cctgaaacgg gtatgcagct tgccgaacag attagaacat ctcctcaatc tattgctaag ctcgcgggct ttaaaatatg gtttataaag agtccaaaac gtagaatggc tgagaatagt attttgggac tcactgaaaa aattgaaaaa catgcgcaaa ttgtgcgatc tgttagggat aaaattttta tggagcatca aaaagaacaa aaacgtattg aatgcgtagt aaaaaaaccc agcaaaaaaa tacaagatat tcttaatttg ccaaaagatg aacaaaaaaa ggtcttagaa tcctctccta cactttataa agatttctgt agttttctag atgaagtaaa ttctcgtctt acagtagatc agcaaaatta catctttgat aaaaaggaga tagaattttc tgacagtgtt ggcatgtcaa gaaagaaagc aaggatggtt atgaacataa ttgataaaag tgtaagatta cacaagcaaa ttacagcaat taaacctgat tgttcgcaag taatggctat gactatttaa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHMG VLITSPVLNY FYPGTKTLKN KLGIMGYKQL EKRCKRNAKK VINSLRNEPL PETFDSSYLK YLHKRLFGSA FEWAGYTRDL SFAFDDGTIA QMSMMKIPGT DIYFAHGDKI QENLKEFDEI LASKSNLQGL SREDFIEETV KLFSFLNYIH PFRAGNEAVQ HIFFEKLAEA AGHKLDFSVV TEERIMRACN DAMALKGEEA HQAMKSLFED ISNPEEVIIL RDFFKHIPRV ERQRLNDEYV TMPREGTTYT GIYQLGSPNS LLFKTVDSHI LCLKDYFTPE QLKALKSGGK ITFTVPMKKD LDPILIPGEK LAPLKEEEII KRTRRADCIR DHQREIDRYS KLIYGDSTIL DKHMMRIHEY PETGMQLAEQ IRTSPQSIAK LAGFKIWFIK SPKRRMAENS ILGLTEKIEK HAQIVRSVRD KIFMEHQKEQ KRIECVVKKP SKKIQDILNL PKDEQKKVLE SSPTLYKDFC SFLDEVNSRL TVDQQNYIFD KKEIEFSDSV GMSRKKARMV MNIIDKSVRL HKQITAIKPD CSQVMAMTI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggag tactcattac atctccagtg ctgaattatt tttatcctgg tactaagacg ttaaagaata aattgggtat aatgggctat aaacaacttg aaaaaagatg caagcgtaat gcgaaaaaag tgatcaatag tctgcgtaac gaaccactac cagaaacatt tgattcttcc tatctaaaat atcttcataa acgcctgttc gggagtgcat tcgaatgggc tggatatacc cgtgatcttt catttgcatt tgatgatggt actattgctc aaatgtcaat gatgaaaatt ccgggaacag atatttattt tgcacatggt gataaaattc aagaaaactt aaaggaattt gatgaaatac ttgcttcgaa gagtaatttg caaggtttat cgcgtgaaga ttttattgag gaaacagtaa agcttttttc ttttctcaac tatatacatc cttttagagc tggtaatgaa gctgtacagc acatattttt tgaaaaactt gctgaagctg cgggccataa gcttgatttt tcggttgtga cagaagagcg tataatgcgt gcctgtaatg atgcaatggc tctaaagggc gaggaagccc atcaagcaat gaagtctttg tttgaagata tctctaatcc agaagaagta attattttaa gagatttctt taaacacata cctagagttg agcgccaacg tttgaatgat gaatatgtta caatgccgcg ggaaggtaca acttatacag gtatttatca actaggtagt ccaaattctc ttctgtttaa aacggtggat tcgcatattc tatgtctgaa agattatttt acgccagaac aactcaaggc attaaaatct ggtggtaaga ttacatttac agttccaatg aaaaaagact tagatccaat tctcattcca ggagaaaaat tagctccttt gaaggaagaa gaaataatca aacggactag aagggctgat tgtattagag accaccaaag agaaattgat cgttactcaa agctcattta tggagacagc acgatattag ataaacatat gatgcgtatt catgaatatc ctgaaacggg tatgcagctt gccgaacaga ttagaacatc tcctcaatct attgctaagc tcgcgggctt taaaatatgg tttataaaga gtccaaaacg tagaatggct gagaatagta ttttgggact cactgaaaaa attgaaaaac atgcgcaaat tgtgcgatct gttagggata aaatttttat ggagcatcaa aaagaacaaa aacgtattga atgcgtagta aaaaaaccca gcaaaaaaat acaagatatt cttaatttgc caaaagatga acaaaaaaag gtcttagaat cctctcctac actttataaa gatttctgta gttttctaga tgaagtaaat tctcgtctta cagtagatca gcaaaattac atctttgata aaaaggagat agaattttct gacagtgttg gcatgtcaag aaagaaagca aggatggtta tgaacataat tgataaaagt gtaagattac acaagcaaat tacagcaatt aaacctgatt gttcgcaagt aatggctatg actatttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BasaA.17330.c.B2.GE36611
| HARVESTED ON: | 2/8/2013 |
| SEQUENCED ON: | 8/22/2013 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV LNYFYPGTKT LKNKLGIMGY KQLEKRCKRN AKKVINSLRN EPLPETFDSS YLKYLHKRLF GSAFEWAGYT RDLSFAFDDG TIAQMSMMKI PGTDIYFAHG DKIQENLKEF DEILASKSNL QGLSREDFIE ETVKLFSFLN YIHPFRAGNE AVQHIFFEKL AEAAGHKLDF SVVTEERIMR ACNDAMALKG EEAHQAMKSL FEDISNPEEV IILRDFFKHI PRVERQRLND E
Validated NT Sequence
atggctcacc accaccacca ccatatggtg ctgaattatt tttatcctgg tactaagacg ttaaagaata aattgggtat aatgggctat aaacaacttg aaaaaagatg caagcgtaat gcgaaaaaag tgatcaatag tctgcgtaac gaaccactac cagaaacatt tgattcttcc tatctaaaat atcttcataa acgcctgttc gggagtgcat tcgaatgggc tggatatacc cgtgatcttt catttgcatt tgatgatggt actattgctc aaatgtcaat gatgaaaatt ccgggaacag atatttattt tgcacatggt gataaaattc aagaaaactt aaaggaattt gatgaaatac ttgcttcgaa gagtaatttg caaggtttat cgcgtgaaga ttttattgag gaaacagtaa agcttttttc ttttctcaac tatatacatc cttttagagc tggtaatgaa gctgtacagc acatattttt tgaaaaactt gctgaagctg cgggccataa gcttgatttt tcggttgtga cagaagagcg tataatgcgt gcctgtaatg atgcaatggc tctaaagggc gaggaagccc atcaagcaat gaagtctttg tttgaagata tctctaatcc agaagaagta attattttaa gagatttctt taaacacata cctagagttg agcgccaacg tttgaatgat gaataagtga gtaagat
Expected Protein Sequence
MAHHHHHHMV LNYFYPGTKT LKNKLGIMGY KQLEKRCKRN AKKVINSLRN EPLPETFDSS YLKYLHKRLF GSAFEWAGYT RDLSFAFDDG TIAQMSMMKI PGTDIYFAHG DKIQENLKEF DEILASKSNL QGLSREDFIE ETVKLFSFLN YIHPFRAGNE AVQHIFFEKL AEAAGHKLDF SVVTEERIMR ACNDAMALKG EEAHQAMKSL FEDISNPEEV IILRDFFKHI PRVERQRLND E
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc tgaattattt ttatcctggt actaagacgt taaagaataa attgggtata atgggctata aacaacttga aaaaagatgc aagcgtaatg cgaaaaaagt gatcaatagt ctgcgtaacg aaccactacc agaaacattt gattcttcct atctaaaata tcttcataaa cgcctgttcg ggagtgcatt cgaatgggct ggatataccc gtgatctttc atttgcattt gatgatggta ctattgctca aatgtcaatg atgaaaattc cgggaacaga tatttatttt gcacatggtg ataaaattca agaaaactta aaggaatttg atgaaatact tgcttcgaag agtaatttgc aaggtttatc gcgtgaagat tttattgagg aaacagtaaa gcttttttct tttctcaact atatacatcc ttttagagct ggtaatgaag ctgtacagca catatttttt gaaaaacttg ctgaagctgc gggccataag cttgattttt cggttgtgac agaagagcgt ataatgcgtg cctgtaatga tgcaatggct ctaaagggcg aggaagccca tcaagcaatg aagtctttgt ttgaagatat ctctaatcca gaagaagtaa ttattttaag agatttcttt aaacacatac ctagagttga gcgccaacgt ttgaatgatg aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BasaA.17330.c.B3.GE36612
| HARVESTED ON: | 2/8/2013 |
| SEQUENCED ON: | 2/14/2013 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV LNYFYPGTKT LKNKLGIMGY KQLEKRCKRN AKKVINSLRN EPLPETFDSS YLKYLHKRLF GSAFEWAGYT RDLSFAFDDG TIAQMSMMKI PGTDIYFAHG DKIQENLKEF DEILASKSNL QGLSREDFIE ETVKLFSFLN YIHPFRAGNE AVQHIFFEKL AEAAGHKLDF SVVTEERIMR ACNDAMALKG EEAHQAMKSL FEDISNPEEV IILRDFFKHI PRVERQRLND EYVTMPREGT TYTGIYQLXS PNSLLXKTVD SHILCLKXXF TPEQLXALKS GGXITFT
Validated NT Sequence
ttnnnnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatggtgctg aattattttt atcctggtac taagacgtta aagaataaat tgggtataat gggctataaa caacttgaaa aaagatgcaa gcgtaatgcg aaaaaagtga tcaatagtct gcgtaacgaa ccactaccag aaacatttga ttcttcctat ctaaaatatc ttcataaacg cctgttcggg agtgcattcg aatgggctgg atatacccgt gatctttcat ttgcatttga tgatggtact attgctcaaa tgtcaatgat gaaaattccg ggaacagata tttattttgc acatggtgat aaaattcaag aaaacttaaa ggaatttgat gaaatacttg cttcgaagag taatttgcaa ggtttatcgc gtgaagattt tattgaggaa acagtaaagc ttttttcttt tctcaactat atacatcctt ttagagctgg taatgaagct gtacagcaca tattttttga aaaacttgct gaagctgcgg gccataagct tgatttttcg gttgtgacag aagagcgtat aatgcgtgcc tgtaatgatg caatggctct aaagggcgag gaagcccatc aagcaatgaa gtctttgttt gaagatatct ctaatccaga agaagtaatt attttaagag atttctttaa acacatacct agagttgagc gccaacgttt gaatgatgaa tatgttacaa tgccgcggga aggtacaact tatacaggta tttatcaact angtagtcca aattctcttc tgnttaaaac ggtggattcg catattctat gtctgaaaga ntantttacg ccagaacaac tcnaggcatt aaaatctggt ggtaanatta catttacnta antgantaan annnnnnnnn nnnnnnnnnn cccnnnnnnn nnnnnnnnnn nnnnnaaana annnnncnan ngnnncnnaa ntnnnnnann annnnnnnna nncccnnnac nnnccnnnnn nncnnnnnnn nnnnnnnncn nnnnnnnacn nncnnnnnnn nnnnnna
Expected Protein Sequence
MAHHHHHHMV LNYFYPGTKT LKNKLGIMGY KQLEKRCKRN AKKVINSLRN EPLPETFDSS YLKYLHKRLF GSAFEWAGYT RDLSFAFDDG TIAQMSMMKI PGTDIYFAHG DKIQENLKEF DEILASKSNL QGLSREDFIE ETVKLFSFLN YIHPFRAGNE AVQHIFFEKL AEAAGHKLDF SVVTEERIMR ACNDAMALKG EEAHQAMKSL FEDISNPEEV IILRDFFKHI PRVERQRLND EYVTMPREGT TYTGIYQLGS PNSLLFKTVD SHILCLKDYF TPEQLKALKS GGKITFT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc tgaattattt ttatcctggt actaagacgt taaagaataa attgggtata atgggctata aacaacttga aaaaagatgc aagcgtaatg cgaaaaaagt gatcaatagt ctgcgtaacg aaccactacc agaaacattt gattcttcct atctaaaata tcttcataaa cgcctgttcg ggagtgcatt cgaatgggct ggatataccc gtgatctttc atttgcattt gatgatggta ctattgctca aatgtcaatg atgaaaattc cgggaacaga tatttatttt gcacatggtg ataaaattca agaaaactta aaggaatttg atgaaatact tgcttcgaag agtaatttgc aaggtttatc gcgtgaagat tttattgagg aaacagtaaa gcttttttct tttctcaact atatacatcc ttttagagct ggtaatgaag ctgtacagca catatttttt gaaaaacttg ctgaagctgc gggccataag cttgattttt cggttgtgac agaagagcgt ataatgcgtg cctgtaatga tgcaatggct ctaaagggcg aggaagccca tcaagcaatg aagtctttgt ttgaagatat ctctaatcca gaagaagtaa ttattttaag agatttcttt aaacacatac ctagagttga gcgccaacgt ttgaatgatg aatatgttac aatgccgcgg gaaggtacaa cttatacagg tatttatcaa ctaggtagtc caaattctct tctgtttaaa acggtggatt cgcatattct atgtctgaaa gattatttta cgccagaaca actcaaggca ttaaaatctg gtggtaagat tacatttaca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BasaA.17330.c.B3.PS01856
| PURIFICATION DATe: | 7/3/2013 |
| CONCENTRATION: | 21mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV LNYFYPGTKT LKNKLGIMGY KQLEKRCKRN AKKVINSLRN EPLPETFDSS YLKYLHKRLF GSAFEWAGYT RDLSFAFDDG TIAQMSMMKI PGTDIYFAHG DKIQENLKEF DEILASKSNL QGLSREDFIE ETVKLFSFLN YIHPFRAGNE AVQHIFFEKL AEAAGHKLDF SVVTEERIMR ACNDAMALKG EEAHQAMKSL FEDISNPEEV IILRDFFKHI PRVERQRLND EYVTMPREGT TYTGIYQLXS PNSLLXKTVD SHILCLKXXF TPEQLXALKS GGXITFT
Validated NT Sequence
ttnnnnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatggtgctg aattattttt atcctggtac taagacgtta aagaataaat tgggtataat gggctataaa caacttgaaa aaagatgcaa gcgtaatgcg aaaaaagtga tcaatagtct gcgtaacgaa ccactaccag aaacatttga ttcttcctat ctaaaatatc ttcataaacg cctgttcggg agtgcattcg aatgggctgg atatacccgt gatctttcat ttgcatttga tgatggtact attgctcaaa tgtcaatgat gaaaattccg ggaacagata tttattttgc acatggtgat aaaattcaag aaaacttaaa ggaatttgat gaaatacttg cttcgaagag taatttgcaa ggtttatcgc gtgaagattt tattgaggaa acagtaaagc ttttttcttt tctcaactat atacatcctt ttagagctgg taatgaagct gtacagcaca tattttttga aaaacttgct gaagctgcgg gccataagct tgatttttcg gttgtgacag aagagcgtat aatgcgtgcc tgtaatgatg caatggctct aaagggcgag gaagcccatc aagcaatgaa gtctttgttt gaagatatct ctaatccaga agaagtaatt attttaagag atttctttaa acacatacct agagttgagc gccaacgttt gaatgatgaa tatgttacaa tgccgcggga aggtacaact tatacaggta tttatcaact angtagtcca aattctcttc tgnttaaaac ggtggattcg catattctat gtctgaaaga ntantttacg ccagaacaac tcnaggcatt aaaatctggt ggtaanatta catttacnta antgantaan annnnnnnnn nnnnnnnnnn cccnnnnnnn nnnnnnnnnn nnnnnaaana annnnncnan ngnnncnnaa ntnnnnnann annnnnnnna nncccnnnac nnnccnnnnn nncnnnnnnn nnnnnnnncn nnnnnnnacn nncnnnnnnn nnnnnna
Expressed Protein Sequence
MAHHHHHHMV LNYFYPGTKT LKNKLGIMGY KQLEKRCKRN AKKVINSLRN EPLPETFDSS YLKYLHKRLF GSAFEWAGYT RDLSFAFDDG TIAQMSMMKI PGTDIYFAHG DKIQENLKEF DEILASKSNL QGLSREDFIE ETVKLFSFLN YIHPFRAGNE AVQHIFFEKL AEAAGHKLDF SVVTEERIMR ACNDAMALKG EEAHQAMKSL FEDISNPEEV IILRDFFKHI PRVERQRLND EYVTMPREGT TYTGIYQLGS PNSLLFKTVD SHILCLKDYF TPEQLKALKS GGKITFT
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTGC TGAATTATTT TTATCCTGGT ACTAAGACGT TAAAGAATAA ATTGGGTATA ATGGGCTATA AACAACTTGA AAAAAGATGC AAGCGTAATG CGAAAAAAGT GATCAATAGT CTGCGTAACG AACCACTACC AGAAACATTT GATTCTTCCT ATCTAAAATA TCTTCATAAA CGCCTGTTCG GGAGTGCATT CGAATGGGCT GGATATACCC GTGATCTTTC ATTTGCATTT GATGATGGTA CTATTGCTCA AATGTCAATG ATGAAAATTC CGGGAACAGA TATTTATTTT GCACATGGTG ATAAAATTCA AGAAAACTTA AAGGAATTTG ATGAAATACT TGCTTCGAAG AGTAATTTGC AAGGTTTATC GCGTGAAGAT TTTATTGAGG AAACAGTAAA GCTTTTTTCT TTTCTCAACT ATATACATCC TTTTAGAGCT GGTAATGAAG CTGTACAGCA CATATTTTTT GAAAAACTTG CTGAAGCTGC GGGCCATAAG CTTGATTTTT CGGTTGTGAC AGAAGAGCGT ATAATGCGTG CCTGTAATGA TGCAATGGCT CTAAAGGGCG AGGAAGCCCA TCAAGCAATG AAGTCTTTGT TTGAAGATAT CTCTAATCCA GAAGAAGTAA TTATTTTAAG AGATTTCTTT AAACACATAC CTAGAGTTGA GCGCCAACGT TTGAATGATG AATATGTTAC AATGCCGCGG GAAGGTACAA CTTATACAGG TATTTATCAA CTAGGTAGTC CAAATTCTCT TCTGTTTAAA ACGGTGGATT CGCATATTCT ATGTCTGAAA GATTATTTTA CGCCAGAACA ACTCAAGGCA TTAAAATCTG GTGGTAAGAT TACATTTACA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for BasaA.17330.c.B2.PW37292
| PURIFICATION DATe: | 5/20/2013 |
| CONCENTRATION: | 42.3mg/ml |
| OBSERVED MW: | 26kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV LNYFYPGTKT LKNKLGIMGY KQLEKRCKRN AKKVINSLRN EPLPETFDSS YLKYLHKRLF GSAFEWAGYT RDLSFAFDDG TIAQMSMMKI PGTDIYFAHG DKIQENLKEF DEILASKSNL QGLSREDFIE ETVKLFSFLN YIHPFRAGNE AVQHIFFEKL AEAAGHKLDF SVVTEERIMR ACNDAMALKG EEAHQAMKSL FEDISNPEEV IILRDFFKHI PRVERQRLND E
Validated NT Sequence
atggctcacc accaccacca ccatatggtg ctgaattatt tttatcctgg tactaagacg ttaaagaata aattgggtat aatgggctat aaacaacttg aaaaaagatg caagcgtaat gcgaaaaaag tgatcaatag tctgcgtaac gaaccactac cagaaacatt tgattcttcc tatctaaaat atcttcataa acgcctgttc gggagtgcat tcgaatgggc tggatatacc cgtgatcttt catttgcatt tgatgatggt actattgctc aaatgtcaat gatgaaaatt ccgggaacag atatttattt tgcacatggt gataaaattc aagaaaactt aaaggaattt gatgaaatac ttgcttcgaa gagtaatttg caaggtttat cgcgtgaaga ttttattgag gaaacagtaa agcttttttc ttttctcaac tatatacatc cttttagagc tggtaatgaa gctgtacagc acatattttt tgaaaaactt gctgaagctg cgggccataa gcttgatttt tcggttgtga cagaagagcg tataatgcgt gcctgtaatg atgcaatggc tctaaagggc gaggaagccc atcaagcaat gaagtctttg tttgaagata tctctaatcc agaagaagta attattttaa gagatttctt taaacacata cctagagttg agcgccaacg tttgaatgat gaataagtga gtaagat
Expressed Protein Sequence
MAHHHHHHMV LNYFYPGTKT LKNKLGIMGY KQLEKRCKRN AKKVINSLRN EPLPETFDSS YLKYLHKRLF GSAFEWAGYT RDLSFAFDDG TIAQMSMMKI PGTDIYFAHG DKIQENLKEF DEILASKSNL QGLSREDFIE ETVKLFSFLN YIHPFRAGNE AVQHIFFEKL AEAAGHKLDF SVVTEERIMR ACNDAMALKG EEAHQAMKSL FEDISNPEEV IILRDFFKHI PRVERQRLND E
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTGC TGAATTATTT TTATCCTGGT ACTAAGACGT TAAAGAATAA ATTGGGTATA ATGGGCTATA AACAACTTGA AAAAAGATGC AAGCGTAATG CGAAAAAAGT GATCAATAGT CTGCGTAACG AACCACTACC AGAAACATTT GATTCTTCCT ATCTAAAATA TCTTCATAAA CGCCTGTTCG GGAGTGCATT CGAATGGGCT GGATATACCC GTGATCTTTC ATTTGCATTT GATGATGGTA CTATTGCTCA AATGTCAATG ATGAAAATTC CGGGAACAGA TATTTATTTT GCACATGGTG ATAAAATTCA AGAAAACTTA AAGGAATTTG ATGAAATACT TGCTTCGAAG AGTAATTTGC AAGGTTTATC GCGTGAAGAT TTTATTGAGG AAACAGTAAA GCTTTTTTCT TTTCTCAACT ATATACATCC TTTTAGAGCT GGTAATGAAG CTGTACAGCA CATATTTTTT GAAAAACTTG CTGAAGCTGC GGGCCATAAG CTTGATTTTT CGGTTGTGAC AGAAGAGCGT ATAATGCGTG CCTGTAATGA TGCAATGGCT CTAAAGGGCG AGGAAGCCCA TCAAGCAATG AAGTCTTTGT TTGAAGATAT CTCTAATCCA GAAGAAGTAA TTATTTTAAG AGATTTCTTT AAACACATAC CTAGAGTTGA GCGCCAACGT TTGAATGATG AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT