SSGCID: Target Page

BasaA.17330.b

Bep3 protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BasaA.17330.b
ORGANISM:	Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BasaA.17330.b.B2.GE36609	FIC		90	330
BasaA.17330.b.B5.GE37448	N-term extended FIC		1	330

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	E6YQP7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKSYSSTSLS VEELQKRRQI VEAAVHTHTI ENIALHSDTI TILEQYAKGN YSLNEFNAIM DNFEKTILGD VKKVRVEDAE LPFHSDRPSS YNYVYPFTFV LKNKYGVADM EILSTVGGHS TVKALVNLYH EPLPEKFDST YLKYLHRRLF ENTFEWAGHS RDLPFVFDDG TIAIMPTMRK ISKESDFRNK KGDGFVESAK IVGCFDYMDK TLAEQNNLQG LSRKDFVNKA AHMFAFLNYV HPFRDGNGRT QRIFFEKLAE AAGHKLDFSV VTRKRMVVCS KLSMGCADTS DDISVMRHMF EDISNPKTVS IMKEFISSMN NIEYQEAQKK IIVMPNKGDS YVGVYENDSP ESILVKVDRD YILEPIYMIF KKDYLSPTQI KALKSGDTLN FVFPTNEDIK SVLIPEEILA PLTSDQISQR VMVHPTILSK ERDVNIYARR VYKQVKEFNE KMALINKNEN FDKVLIKQIT DSPESISKLA GKKILGFKNS KYKTAQRNIE ALTQQISGYG ATVRDVRHEI VQEHLVQQKR LMTVVKMPSK ELQDIFNLSE DMQKEALNFS PSLQKELDTF IREVRSRLTP SEHKWLCDAK YTLLAESIGI SESKAKTIQK LFVQGKQLQS LLKSVKLNDV GAINMAS

NT Sequence

atgaaatcgt atagttcgac atccctatca gtggaagagc tccaaaagcg tcgtcaaatc gttgaggcag cggttcatac tcatactatt gaaaacatcg ctttgcattc ggatacaatt acaattttag aacaatatgc aaagggaaat tattcattga atgaatttaa tgctatcatg gataattttg agaaaactat tctaggtgat gtaaaaaaag tgcgagtgga agacgctgag ctaccatttc actctgaccg cccgtcatcc tacaattatg tttatccttt cacctttgta ttaaaaaata aatatggggt agcagacatg gaaattctgt ccacagtagg cggacattcc acggtaaaag cactggttaa tttataccat gaaccattac cggaaaagtt tgattccact tatttaaaat atcttcatag acgcttgttc gagaatacat ttgaatgggc tggacacagc cgtgaccttc catttgtatt tgatgatggt actattgcca ttatgccaac aatgagaaag ataagcaagg aaagcgattt tagaaacaaa aaaggtgatg gttttgtaga aagtgccaaa attgttggct gcttcgatta tatggataaa acacttgcag aacagaataa tttgcaaggt ttatcgcgta aagattttgt taataaagca gcgcatatgt ttgcttttct taattatgtc catcccttta gagatgggaa tggtcgcacg caacgaatat tttttgaaaa attagcagaa gctgcaggcc ataagctcga tttttcggtt gtaacaagaa aacgcatggt agtttgcagt aagctctcca tggggtgtgc tgatacttcg gacgatataa gtgttatgag acatatgttt gaagatattt caaatccaaa aacagtaagt attatgaagg agttcataag ctccatgaat aatatcgagt atcaagaggc tcaaaaaaag attattgtaa tgccaaataa aggggatagc tatgtaggcg tttatgaaaa tgatagcccg gaatctattc tagtcaaagt ggatcgtgat tatattttag aaccaattta tatgatattt aaaaaagatt atctgtcacc aacgcaaatt aaggcattaa aatctggtga tacactaaat tttgtatttc caacaaacga agatataaaa agcgttctta ttccagaaga aatattagct cctttaacat cagatcaaat tagtcaaagg gttatggtgc atcctactat cttatcaaaa gaaagagacg ttaatattta tgcaagacgt gtttataaac aggtaaaaga attcaacgaa aaaatggctc tcattaataa aaatgaaaat tttgataaag tgcttataaa gcaaataact gattctcctg aatctatttc taaattagca ggtaaaaaaa tattaggctt taaaaattca aaatacaaaa cagctcaacg aaatattgaa gcactgactc aacagattag tggctatggc gctactgtaa gagatgtgag gcatgaaatt gttcaagagc atctagtcca acaaaaacgt ttgatgacag tagtgaaaat gcccagcaaa gaattacaag atattttcaa tttgtcggag gatatgcaga aagaagcttt aaatttctct ccttcattgc aaaaagaact cgatactttt atacgagaag ttcgttcccg tcttacacca agcgagcata aatggctatg tgatgctaag tatacattac tagccgaaag cattggtata tcagaaagta aagcgaagac aattcaaaag ctttttgtgc aaggcaagca actacagagc ttgttaaaaa gtgtcaagct taatgatgta ggagcaataa atatggctag ttaa

Details for BasaA.17330.b.B2.GE36609

HARVESTED ON:	2/8/2013
SEQUENCED ON:	2/14/2013
EXPECTED MW:	29kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS YNYVYPFTFV LKNKYGVADM EILSTVGGHS TVKALVNLYH EPLPEKFDST YLKYLHRRLF ENTFEWAGHS RDLPFVFDDG TIAIMPTMRK ISKESDFRNK KGDGFVESAK IVGCFDYMDK TLAEQNNLQG LSRKDFVNKA AHMFAFLNYV HPFRDGNGRT QRIFFEKLAE AAGHKLDFSV VTRKRMVVCS KLSMGCADTS DDISVMRHMF EDISNPKTVS IMKEFISSMN NIEYQEAQKK

Validated NT Sequence

tttnnnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgtccta caattatgtt tatcctttca cctttgtatt aaaaaataaa tatggggtag cagacatgga aattctgtcc acagtaggcg gacattccac ggtaaaagca ctggttaatt tataccatga accattaccg gaaaagtttg attccactta tttaaaatat cttcatagac gcttgttcga gaatacattt gaatgggctg gacacagccg tgaccttcca tttgtatttg atgatggtac tattgccatt atgccaacaa tgagaaagat aagcaaggaa agcgatttta gaaacaaaaa aggtgatggt tttgtagaaa gtgccaaaat tgttggctgc ttcgattata tggataaaac acttgcagaa cagaataatt tgcaaggttt atcgcgtaaa gattttgtta ataaagcagc gcatatgttt gcttttctta attatgtcca tccctttaga gatgggaatg gtcgcacgca acgaatattt tttgaaaaat tagcagaagc tgcaggccat aagctcgatt tttcggttgt aacaagaaaa cgcatggtag tttgcagtaa gctctccatg gggtgtgctg atacttcgga cgatataagt gttatgagac atatgtttga agatatttca aatccaaaaa cagtaagtat tatgaaggag ttcataagct ccatgaataa tatcgagtat caagaggctc aaaaaaagta agtgagtaag ataggatccg gnnnnnnnnn nccccnnnnn nnaaaaaann nnnnnnnnng tnnncnnntn ctcnnnnnnn nnnnnnnnnn nnnnanncnn ncncccnnnc ngcnnnnnnn nanncnnncn tnnccnnnnn ntntngnnnn nnnnnnncnn nnnnnncncn nnnnnncnnn nnnttc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcct acaattatgt ttatcctttc acctttgtat taaaaaataa atatggggta gcagacatgg aaattctgtc cacagtaggc ggacattcca cggtaaaagc actggttaat ttataccatg aaccattacc ggaaaagttt gattccactt atttaaaata tcttcataga cgcttgttcg agaatacatt tgaatgggct ggacacagcc gtgaccttcc atttgtattt gatgatggta ctattgccat tatgccaaca atgagaaaga taagcaagga aagcgatttt agaaacaaaa aaggtgatgg ttttgtagaa agtgccaaaa ttgttggctg cttcgattat atggataaaa cacttgcaga acagaataat ttgcaaggtt tatcgcgtaa agattttgtt aataaagcag cgcatatgtt tgcttttctt aattatgtcc atccctttag agatgggaat ggtcgcacgc aacgaatatt ttttgaaaaa ttagcagaag ctgcaggcca taagctcgat ttttcggttg taacaagaaa acgcatggta gtttgcagta agctctccat ggggtgtgct gatacttcgg acgatataag tgttatgaga catatgtttg aagatatttc aaatccaaaa acagtaagta ttatgaagga gttcataagc tccatgaata atatcgagta tcaagaggct caaaaaaagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BasaA.17330.b.B5.GE37448

HARVESTED ON:	10/31/2013
SEQUENCED ON:	11/5/2013
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK SYSSTSLSVE ELQKRRQIVE AAVHTHTIEN IALHSDTITI LEQYAKGNYS LNEFNAIMDN FEKTILGDVK KVRVEDAELP FHSDRPSSYN YVYPFTFVLK NKYGVADMEI LSTVGGHSTV KALVNLYHEP LPEKFDSTYL KYLHRRLFEN TFEWAGHSRD LPFVFDDGTI AIMPTMRKIS KESDFRNKKG DGFVESAKIV GCFDYMDKTL AEQNNLQGLS RKDFVNKAAH MFAFLNYVHP FRDGNGRTQR IFFEKLAEAA GHKLDFSVVT RKRMVVCSKL SMGCADTSDD ISVMRHMFED ISNPKTVSIM KEFISSMNNI EYQEAQKK

Validated NT Sequence

tcttactcac ttactttttt tgagcctctt gatactcgat attattcatg gagcttatga actccttcat aatacttact gtttttggat ttgaaatatc ttcaaacata tgtctcataa cacttatatc gtccgaagta tcagcacacc ccatggagag cttactgcaa actaccatgc gttttcttgt tacaaccgaa aaatcgagct tatggcctgc agcttctgct aatttttcaa aaaatattcg ttgcgtgcga ccattcccat ctctaaaggg atggacataa ttaagaaaag caaacatatg cgctgcttta ttaacaaaat ctttacgcga taaaccttgc aaattattct gttctgcaag tgttttatcc atataatcga agcagccaac aattttggca ctttctacaa aaccatcacc ttttttgttt ctaaaatcgc tttccttgct tatctttctc attgttggca taatggcaat agtaccatca tcaaatacaa atggaaggtc acggctgtgt ccagcccatt caaatgtatt ctcgaacaag cgtctatgaa gatattttaa ataagtggaa tcaaactttt ccggtaatgg ttcatggtat aaattaacca gtgcttttac cgtggaatgt ccgcctactg tggacagaat ttccatgtct gctaccccat atttattttt taatacaaag gtgaaaggat aaacataatt gtaggatgac gggcggtcag agtgaaatgg tagctcagcg tcttccactc gcactttttt tacatcacct agaatagttt tctcaaaatt atccatgata gcattaaatt cattcaatga ataatttccc tttgcatatt gttctaaaat tgtaattgta tccgaatgca aagcgatgtt ttcaatagta tgagtatgaa ccgctgcctc aacgatttga cgacgctttt ggagctcttc cactgatagg gatgtcgaac tatacgattt catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat cgtatagttc gacatcccta tcagtggaag agctccaaaa gcgtcgtcaa atcgttgagg cagcggttca tactcatact attgaaaaca tcgctttgca ttcggataca attacaattt tagaacaata tgcaaaggga aattattcat tgaatgaatt taatgctatc atggataatt ttgagaaaac tattctaggt gatgtaaaaa aagtgcgagt ggaagacgct gagctaccat ttcactctga ccgcccgtca tcctacaatt atgtttatcc tttcaccttt gtattaaaaa ataaatatgg ggtagcagac atggaaattc tgtccacagt aggcggacat tccacggtaa aagcactggt taatttatac catgaaccat taccggaaaa gtttgattcc acttatttaa aatatcttca tagacgcttg ttcgagaata catttgaatg ggctggacac agccgtgacc ttccatttgt atttgatgat ggtactattg ccattatgcc aacaatgaga aagataagca aggaaagcga ttttagaaac aaaaaaggtg atggttttgt agaaagtgcc aaaattgttg gctgcttcga ttatatggat aaaacacttg cagaacagaa taatttgcaa ggtttatcgc gtaaagattt tgttaataaa gcagcgcata tgtttgcttt tcttaattat gtccatccct ttagagatgg gaatggtcgc acgcaacgaa tattttttga aaaattagca gaagctgcag gccataagct cgatttttcg gttgtaacaa gaaaacgcat ggtagtttgc agtaagctct ccatggggtg tgctgatact tcggacgata taagtgttat gagacatatg tttgaagata tttcaaatcc aaaaacagta agtattatga aggagttcat aagctccatg aataatatcg agtatcaaga ggctcaaaaa aagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat