BasaA.17330.a

Bep4 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BasaA.17330.a
ORGANISM: Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BasaA.17330.a.B1.GE35932 full length 1 624
BasaA.17330.a.B2.GE36607 FIC 85 313
BasaA.17330.a.B3.GE36608 FIC and OB-Fold 85 368
BasaA.17330.a.B5.GE37447 N-term extended FIC 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BasaA.17330.a.B1.PS01725 full length 1 624

External Resources

RESOURCE REFERENCE ID
UniProt: E6YQI1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFKRKIQEQA VSKYASDYAI PRSLMIKRRA TVNSVISACM LEGYILAPKT LDILKKYASG DYSLKQFNTQ MDSSVKEVKV RKKYSTHGVI TTNGMLVNKY GITDAVALHK RSAHDIAKAI VNLSCEEVPE QFDSSYLKCL HKRLFGNMFE WAGQTRDEIS YLEDIKKTKL LPMEKLSSVS SKEIQERFKK IDKILFEKNN LKDLSCEEFS HNVARIFSLL NDTYPFISGN ECTQMVFFEQ LAQVANYKLD FSVITKERMK LVSDAAKTLK GNIGDLAPIE HFFEDVSDPN KVCMLKEFIK NNKCQDKNIK NQIIVAPRKD IVYGGICRNW SSNAILVETE DAYVVCHKDY LMAEELKTLK LGDKMSFTAV IPEDFGDILI PEGVLVSLAK EEIIKRVKKD SFVQRSLENV EYYSRVVYDD SEILKKNMEF INEFVCFDTQ VAKRNTERLV DKIMESPQSI HVLAGVEIFG MKNSKRKKAE ECCGDLRLAI NQYIDNVIYA RDAILQQYEA HNSFKRKAVA MPHKEIQDVL NLPENMRQEV LDTSCSLQQE ICDFVSDVMK RLSFSEYQAL HDNNYEKLAQ NLGVSVNKAE KITKLFKAGE KVYLQAKNLK INHSESMAIT QRAS
NT Sequence
atgtttaaaa gaaagataca ggaacaagca gtttcgaaat atgcttcgga ttatgctata cctcgaagtc taatgataaa gcgtcgtgca acagttaatt cagtcatcag tgcgtgtatg cttgaagggt atattcttgc ccctaaaacg ttagacattt taaagaaata tgcgagcgga gattattcac taaagcagtt taacactcag atggattctt ctgtgaaaga agtaaaggtg cgaaagaaat attcaaccca tggtgttatt actactaatg gaatgctggt aaataaatat ggaataacag atgcagttgc tcttcataaa agaagtgcac atgatatagc aaaagcaatt gttaatctat cttgtgaaga ggtaccagaa caatttgatt cctcttatct taaatgtctt cataagcgct tatttgggaa tatgtttgag tgggctgggc agacacgtga cgaaatttca tatttagagg atataaaaaa aaccaagctg ttaccaatgg aaaaattgag ctctgtaagc agtaaggaaa ttcaagaacg ttttaaaaaa attgacaaaa ttcttttcga gaagaataat ctgaaagatt tatcgtgcga agaattctct cataatgtag caagaatctt ttctttgctt aatgatacat acccttttat cagtgggaat gaatgtacgc aaatggtgtt ttttgagcag cttgctcaag ttgccaacta taaacttgat ttttcagtta taacaaaaga acgcatgaaa cttgtcagcg atgctgcaaa aactttaaaa ggcaacatag gcgatctagc tcctatagaa cacttttttg aagatgtttc tgatccaaac aaagtgtgta tgctgaaaga gtttatcaaa aataataaat gtcaagataa aaatataaaa aatcagatta ttgtagctcc tcgtaaagat attgtttatg gtggtatctg tagaaactgg agttcaaatg ccatcctagt cgaaacggaa gatgcgtacg tcgtatgtca taaagattat ttaatggcag aagaattaaa aacattaaaa ttaggtgata aaatgtcttt tacagctgta atacctgagg attttggaga cattctcatt ccagaaggag tattagtttc tttggcgaaa gaagaaatta ttaaaagagt taaaaaagat tcttttgttc aaagaagctt ggaaaatgtt gagtattatt caagagttgt ttatgatgat tcagaaatat tgaaaaaaaa tatggaattt attaatgaat ttgtatgttt tgatacgcag gttgcgaagc ggaatacgga gaggcttgtg gataaaatta tggaatctcc tcaatctatt catgtgcttg caggtgttga gatatttggt atgaaaaatt caaagcgcaa gaaagctgaa gaatgttgtg gtgaccttag gcttgcaatt aatcaatata tagataatgt aatatacgct agagatgcaa ttttacaaca atacgaagca cacaatagtt ttaaaaggaa agctgtagca atgccccata aggaaataca agatgtttta aatttgccag aaaatatgcg gcaagaagta ttagacactt cttgttcatt acagcaggag atttgtgatt ttgtatctga cgtcatgaag cgtctttcat ttagcgagta tcaagcactt catgataata attatgaaaa gctcgctcaa aatttgggcg tgtcagtgaa taaagcagag aaaattacaa aactttttaa agcgggtgag aaagtgtatc tgcaagctaa aaatctcaaa attaatcact cagaaagtat ggctataacc cagagagcca gttaa
Details for BasaA.17330.a.B1.GE35932
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 72kDa
OBSERVED MW: 72kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 42
Validated AA Sequence
MAHHHHHHMF KRKIQEQAVS KYASDYAIPR SLMIKRRATV NSVISACMLE GYILAPKTLD ILKKYASGDY SLKQFNTQMD SSVKEVKVRK KYSTHGVITT NGMLVNKYGI TDAVALHKRS AHDIAKAIVN LSCEEVPEQF DSSYLKCLHK RLFGNMFEWA GQTRDEISYL EDIKKTKLLP MEKLSSVSSK EIQERFKKID KILFEKNNLK DLSCEEFSHN VARIFSLLND TYPFISGNEC TQMVFFEQLA QVANYKLDFS VITXNA
Validated NT Sequence
tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttta aaagaaagat acaggaacaa gcagtttcga aatatgcttc ggattatgct atacctcgaa gtctaatgat aaagcgtcgt gcaacagtta attcagtcat cagtgcgtgt atgcttgaag ggtatattct tgcccctaaa acgttagaca ttttaaagaa atatgcgagc ggagattatt cactaaagca gtttaacact cagatggatt cttctgtgaa agaagtaaag gtgcgaaaga aatattcaac ccatggtgtt attactacta atggaatgct ggtaaataaa tatggaataa cagatgcagt tgctcttcat aaaagaagtg cacatgatat agcaaaagca attgttaatc tatcttgtga agaggtacca gaacaatttg attcctctta tcttaaatgt cttcataagc gcttatttgg gaatatgttt gagtgggctg ggcagacacg tgacgaaatt tcatatttag aggatataaa aaaaaccaag ctgttaccaa tggaaaaatt gagctctgta agcagtaagg aaattcaaga acgttttaaa aaaattgaca aaattctttt cgagaagaat aatctgaaag atttatcgtg cgaagaattc tctcataatg tagcaagaat cttttctttg cttaatgata catacccttt tatcagtggg aatgaatgta cgcaaatggt gttttttgag cagcttgctc aagttgccaa ctataaactt gatttttcag ttataacaaa naacgcatga aacttgtcag cgatgctgcn aaanctttna anngnannnt nnnnatctag ctncnnnnga anactttttg aanangntnn nnatnnaann nnngnnnatg ctgannantt nnnnaaannn nnaangncan nnnaaannnt naaaaannnn annatnnnan nnccncnnan nnnntnnnnn nnnnnnnnnn nnnannnggt nnannnnnnn nnanncnnnn nnnnnnnnnc nnnnnnnnnn naannnnngn nnnnnnnnna nnnnnnnnnn gnannnnnnn tnnnnnnnnn nnnnnnnnnn nnnnnntnnn nnnna
Expected Protein Sequence
MAHHHHHHMF KRKIQEQAVS KYASDYAIPR SLMIKRRATV NSVISACMLE GYILAPKTLD ILKKYASGDY SLKQFNTQMD SSVKEVKVRK KYSTHGVITT NGMLVNKYGI TDAVALHKRS AHDIAKAIVN LSCEEVPEQF DSSYLKCLHK RLFGNMFEWA GQTRDEISYL EDIKKTKLLP MEKLSSVSSK EIQERFKKID KILFEKNNLK DLSCEEFSHN VARIFSLLND TYPFISGNEC TQMVFFEQLA QVANYKLDFS VITKERMKLV SDAAKTLKGN IGDLAPIEHF FEDVSDPNKV CMLKEFIKNN KCQDKNIKNQ IIVAPRKDIV YGGICRNWSS NAILVETEDA YVVCHKDYLM AEELKTLKLG DKMSFTAVIP EDFGDILIPE GVLVSLAKEE IIKRVKKDSF VQRSLENVEY YSRVVYDDSE ILKKNMEFIN EFVCFDTQVA KRNTERLVDK IMESPQSIHV LAGVEIFGMK NSKRKKAEEC CGDLRLAINQ YIDNVIYARD AILQQYEAHN SFKRKAVAMP HKEIQDVLNL PENMRQEVLD TSCSLQQEIC DFVSDVMKRL SFSEYQALHD NNYEKLAQNL GVSVNKAEKI TKLFKAGEKV YLQAKNLKIN HSESMAITQR AS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttta aaagaaagat acaggaacaa gcagtttcga aatatgcttc ggattatgct atacctcgaa gtctaatgat aaagcgtcgt gcaacagtta attcagtcat cagtgcgtgt atgcttgaag ggtatattct tgcccctaaa acgttagaca ttttaaagaa atatgcgagc ggagattatt cactaaagca gtttaacact cagatggatt cttctgtgaa agaagtaaag gtgcgaaaga aatattcaac ccatggtgtt attactacta atggaatgct ggtaaataaa tatggaataa cagatgcagt tgctcttcat aaaagaagtg cacatgatat agcaaaagca attgttaatc tatcttgtga agaggtacca gaacaatttg attcctctta tcttaaatgt cttcataagc gcttatttgg gaatatgttt gagtgggctg ggcagacacg tgacgaaatt tcatatttag aggatataaa aaaaaccaag ctgttaccaa tggaaaaatt gagctctgta agcagtaagg aaattcaaga acgttttaaa aaaattgaca aaattctttt cgagaagaat aatctgaaag atttatcgtg cgaagaattc tctcataatg tagcaagaat cttttctttg cttaatgata catacccttt tatcagtggg aatgaatgta cgcaaatggt gttttttgag cagcttgctc aagttgccaa ctataaactt gatttttcag ttataacaaa agaacgcatg aaacttgtca gcgatgctgc aaaaacttta aaaggcaaca taggcgatct agctcctata gaacactttt ttgaagatgt ttctgatcca aacaaagtgt gtatgctgaa agagtttatc aaaaataata aatgtcaaga taaaaatata aaaaatcaga ttattgtagc tcctcgtaaa gatattgttt atggtggtat ctgtagaaac tggagttcaa atgccatcct agtcgaaacg gaagatgcgt acgtcgtatg tcataaagat tatttaatgg cagaagaatt aaaaacatta aaattaggtg ataaaatgtc ttttacagct gtaatacctg aggattttgg agacattctc attccagaag gagtattagt ttctttggcg aaagaagaaa ttattaaaag agttaaaaaa gattcttttg ttcaaagaag cttggaaaat gttgagtatt attcaagagt tgtttatgat gattcagaaa tattgaaaaa aaatatggaa tttattaatg aatttgtatg ttttgatacg caggttgcga agcggaatac ggagaggctt gtggataaaa ttatggaatc tcctcaatct attcatgtgc ttgcaggtgt tgagatattt ggtatgaaaa attcaaagcg caagaaagct gaagaatgtt gtggtgacct taggcttgca attaatcaat atatagataa tgtaatatac gctagagatg caattttaca acaatacgaa gcacacaata gttttaaaag gaaagctgta gcaatgcccc ataaggaaat acaagatgtt ttaaatttgc cagaaaatat gcggcaagaa gtattagaca cttcttgttc attacagcag gagatttgtg attttgtatc tgacgtcatg aagcgtcttt catttagcga gtatcaagca cttcatgata ataattatga aaagctcgct caaaatttgg gcgtgtcagt gaataaagca gagaaaatta caaaactttt taaagcgggt gagaaagtgt atctgcaagc taaaaatctc aaaattaatc actcagaaag tatggctata acccagagag ccagttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BasaA.17330.a.B2.GE36607
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS THGVITTNGM LVNKYGITDA VALHKRSAHD IAKAIVNLSC EEVPEQFDSS YLKCLHKRLF GNMFEWAGQT RDEISYLEDI KKTKLLPMEK LSSVSSKEIQ ERFKKIDKIL FEKNNLKDLS CEEFSHNVAR IFSLLNDTYP FISGNECTQM VFFEQLAQVA NYKLDFSVIT KERMKLVSDA AKTLKGNIGD LAPIEHFFED VSDPNKVCML KEFIKNNKCQ DKNIKNQI
Validated NT Sequence
ttgtttactt taagaaggag anataccatg gctcaccacc accaccacca tatgtcaacc catggtgtta ttactactaa tggaatgctg gtaaataaat atggaataac agatgcagtt gctcttcata aaagaagtgc acatgatata gcaaaagcaa ttgttaatct atcttgtgaa gaggtaccag aacaatttga ttcctcttat cttaaatgtc ttcataagcg cttatttggg aatatgtttg agtgggctgg gcagacacgt gacgaaattt catatttaga ggatataaaa aaaaccaagc tgttaccaat ggaaaaattg agctctgtaa gcagtaagga aattcaagaa cgttttaaaa aaattgacaa aattcttttc gagaagaata atctgaaaga tttatcgtgc gaagaattct ctcataatgt agcaagaatc ttttctttgc ttaatgatac ataccctttt atcagtggga atgaatgtac gcaaatggtg ttttttgagc agcttgctca agttgccaac tataaacttg atttttcagt tataacaaaa gaacgcatga aacttgtcag cgatgctgca aaaactttaa aaggcaacat aggcgatcta gctcctatag aacacttttt tgaagatgtt tctgatccaa acaaagtgtg tatgctgaaa gagtttatca aaaataataa atgtcaagat aaaaatataa aaaatcagat ttaagtgagt aagataggat ccggctgnnn nnnnncccnn nnnannnnan nnnnnnnaaa aaaaannnnn nntcncctcc nnncnncnnn nntntnnnnn nnnnnntnnn nctctnnnnn nnnnnnnnnn tccnnccntt cnnnncncnc nnnnnnncng ntnnnnnggc nnnnnngna
Expected Protein Sequence
MAHHHHHHMS THGVITTNGM LVNKYGITDA VALHKRSAHD IAKAIVNLSC EEVPEQFDSS YLKCLHKRLF GNMFEWAGQT RDEISYLEDI KKTKLLPMEK LSSVSSKEIQ ERFKKIDKIL FEKNNLKDLS CEEFSHNVAR IFSLLNDTYP FISGNECTQM VFFEQLAQVA NYKLDFSVIT KERMKLVSDA AKTLKGNIGD LAPIEHFFED VSDPNKVCML KEFIKNNKCQ DKNIKNQI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa cccatggtgt tattactact aatggaatgc tggtaaataa atatggaata acagatgcag ttgctcttca taaaagaagt gcacatgata tagcaaaagc aattgttaat ctatcttgtg aagaggtacc agaacaattt gattcctctt atcttaaatg tcttcataag cgcttatttg ggaatatgtt tgagtgggct gggcagacac gtgacgaaat ttcatattta gaggatataa aaaaaaccaa gctgttacca atggaaaaat tgagctctgt aagcagtaag gaaattcaag aacgttttaa aaaaattgac aaaattcttt tcgagaagaa taatctgaaa gatttatcgt gcgaagaatt ctctcataat gtagcaagaa tcttttcttt gcttaatgat acataccctt ttatcagtgg gaatgaatgt acgcaaatgg tgttttttga gcagcttgct caagttgcca actataaact tgatttttca gttataacaa aagaacgcat gaaacttgtc agcgatgctg caaaaacttt aaaaggcaac ataggcgatc tagctcctat agaacacttt tttgaagatg tttctgatcc aaacaaagtg tgtatgctga aagagtttat caaaaataat aaatgtcaag ataaaaatat aaaaaatcag atttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BasaA.17330.a.B3.GE36608
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS THGVITTNGM LVNKYGITDA VALHKRSAHD IAKAIVNLSC EEVPEQFDSS YLKCLHKRLF GNMFEWAGQT RDEISYLEDI KKTKLLPMEK LSSVSSKEIQ ERFKKIDKIL FEKNNLKDLS CEEFSHNVAR IFSLLNDTYP FISGNECTQM VFFEQLAQVA NYKLDFSVIT KERMKLVSDA AKTLKGNIGD LAPIEHFFED VSDPNKVCML KEFIKNNKCQ DKNIKNQIIV APRKDIVYGG ICRNWSSNAI LVETEDAYVV CHKDYLMAEE LKTLKLGDKM SFT
Validated NT Sequence
ttttntnnnn tttaagaagg agnnatacca tggctcacca ccaccaccac catatgtcaa cccatggtgt tattactact aatggaatgc tggtaaataa atatggaata acagatgcag ttgctcttca taaaagaagt gcacatgata tagcaaaagc aattgttaat ctatcttgtg aagaggtacc agaacaattt gattcctctt atcttaaatg tcttcataag cgcttatttg ggaatatgtt tgagtgggct gggcagacac gtgacgaaat ttcatattta gaggatataa aaaaaaccaa gctgttacca atggaaaaat tgagctctgt aagcagtaag gaaattcaag aacgttttaa aaaaattgac aaaattcttt tcgagaagaa taatctgaaa gatttatcgt gcgaagaatt ctctcataat gtagcaagaa tcttttcttt gcttaatgat acataccctt ttatcagtgg gaatgaatgt acgcaaatgg tgttttttga gcagcttgct caagttgcca actataaact tgatttttca gttataacaa aagaacgcat gaaacttgtc agcgatgctg caaaaacttt aaaaggcaac ataggcgatc tagctcctat agaacacttt tttgaagatg tttctgatcc aaacaaagtg tgtatgctga aagagtttat caaaaataat aaatgtcaag ataaaaatat aaaaaatcag attattgtag ctcctcgtaa agatattgtt tatggtggta tctgtagaaa ctggagttca aatgccatcc tagtcgaaac ggaagatgcg tacgtcgtat gtcataaaga ttatttaatg gcagaagaat taaaaacatt aaaattaggt gataaaatgt cttttacata antgagtaag anaggatcnn nnnnnnnnnn nnnnnnnnnn nnnnnntnnn nnnnaanaaa aaaanaannn nnnnnntnnn cnnnnnnnnc nnnnnncnnn nnnnannncn nnnntcnnnn tnc
Expected Protein Sequence
MAHHHHHHMS THGVITTNGM LVNKYGITDA VALHKRSAHD IAKAIVNLSC EEVPEQFDSS YLKCLHKRLF GNMFEWAGQT RDEISYLEDI KKTKLLPMEK LSSVSSKEIQ ERFKKIDKIL FEKNNLKDLS CEEFSHNVAR IFSLLNDTYP FISGNECTQM VFFEQLAQVA NYKLDFSVIT KERMKLVSDA AKTLKGNIGD LAPIEHFFED VSDPNKVCML KEFIKNNKCQ DKNIKNQIIV APRKDIVYGG ICRNWSSNAI LVETEDAYVV CHKDYLMAEE LKTLKLGDKM SFT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa cccatggtgt tattactact aatggaatgc tggtaaataa atatggaata acagatgcag ttgctcttca taaaagaagt gcacatgata tagcaaaagc aattgttaat ctatcttgtg aagaggtacc agaacaattt gattcctctt atcttaaatg tcttcataag cgcttatttg ggaatatgtt tgagtgggct gggcagacac gtgacgaaat ttcatattta gaggatataa aaaaaaccaa gctgttacca atggaaaaat tgagctctgt aagcagtaag gaaattcaag aacgttttaa aaaaattgac aaaattcttt tcgagaagaa taatctgaaa gatttatcgt gcgaagaatt ctctcataat gtagcaagaa tcttttcttt gcttaatgat acataccctt ttatcagtgg gaatgaatgt acgcaaatgg tgttttttga gcagcttgct caagttgcca actataaact tgatttttca gttataacaa aagaacgcat gaaacttgtc agcgatgctg caaaaacttt aaaaggcaac ataggcgatc tagctcctat agaacacttt tttgaagatg tttctgatcc aaacaaagtg tgtatgctga aagagtttat caaaaataat aaatgtcaag ataaaaatat aaaaaatcag attattgtag ctcctcgtaa agatattgtt tatggtggta tctgtagaaa ctggagttca aatgccatcc tagtcgaaac ggaagatgcg tacgtcgtat gtcataaaga ttatttaatg gcagaagaat taaaaacatt aaaattaggt gataaaatgt cttttacatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BasaA.17330.a.B5.GE37447
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 37kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMF KRKIQEQAVS KYASDYAIPR SLMIKRRATV NSVISACMLE GYILAPKTLD ILKKYASGDY SLKQFNTQMD SSVKEVKVRK KYSTHGVITT NGMLVNKYGI TDAVALHKRS AHDIAKAIVN LSCEEVPEQF DSSYLKCLHK RLFGNMFEWA GQTRDEISYL EDIKKTKLLP MEKLSSVSSK EIQERFKKID KILFEKNNLK DLSCEEFSHN VARIFSLLND TYPFISGNEC TQMVFFEQLA QVANYKLDFS VITKERMKLV SDAAKTLKGN IGDLAPIEHF FEDVSDPNKV CMLKEFIKNN KCQDKNIKNQ I
Validated NT Sequence
tcacttaaat ctgatttttt atatttttat cttgacattt attatttttg ataaactctt tcagcataca cactttgttt ggatcagaaa catcttcaaa aaagtgttct ataggagcta gatcgcctat gttgcctttt aaagtttttg cagcatcgct gacaagtttc atgcgttctt ttgttataac tgaaaaatca agtttatagt tggcaacttg agcaagctgc tcaaaaaaca ccatttgcgt acattcattc ccactgataa aagggtatgt atcattaagc aaagaaaaga ttcttgctac attatgagag aattcttcgc acgataaatc tttcagatta ttcttctcga aaagaatttt gtcaattttt ttaaaacgtt cttgaatttc cttactgctt acagagctca atttttccat tggtaacagc ttggtttttt ttatatcctc taaatatgaa atttcgtcac gtgtctgccc agcccactca aacatattcc caaataagcg cttatgaaga catttaagat aagaggaatc aaattgttct ggtacctctt cacaagatag attaacaatt gcttttgcta tatcatgtgc acttctttta tgaagagcaa ctgcatctgt tattccatat ttatttacca gcattccatt agtagtaata acaccatggg ttgaatattt ctttcgcacc tttacttctt tcacagaaga atccatctga gtgttaaact gctttagtga ataatctccg ctcgcatatt tctttaaaat gtctaacgtt ttaggggcaa gaatataccc ttcaagcata cacgcactga tgactgaatt aactgttgca cgacgcttta tcattagact tcgaggtata gcataatccg aagcatattt cgaaactgct tgttcctgta tctttctttt aaacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMF KRKIQEQAVS KYASDYAIPR SLMIKRRATV NSVISACMLE GYILAPKTLD ILKKYASGDY SLKQFNTQMD SSVKEVKVRK KYSTHGVITT NGMLVNKYGI TDAVALHKRS AHDIAKAIVN LSCEEVPEQF DSSYLKCLHK RLFGNMFEWA GQTRDEISYL EDIKKTKLLP MEKLSSVSSK EIQERFKKID KILFEKNNLK DLSCEEFSHN VARIFSLLND TYPFISGNEC TQMVFFEQLA QVANYKLDFS VITKERMKLV SDAAKTLKGN IGDLAPIEHF FEDVSDPNKV CMLKEFIKNN KCQDKNIKNQ I
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttta aaagaaagat acaggaacaa gcagtttcga aatatgcttc ggattatgct atacctcgaa gtctaatgat aaagcgtcgt gcaacagtta attcagtcat cagtgcgtgt atgcttgaag ggtatattct tgcccctaaa acgttagaca ttttaaagaa atatgcgagc ggagattatt cactaaagca gtttaacact cagatggatt cttctgtgaa agaagtaaag gtgcgaaaga aatattcaac ccatggtgtt attactacta atggaatgct ggtaaataaa tatggaataa cagatgcagt tgctcttcat aaaagaagtg cacatgatat agcaaaagca attgttaatc tatcttgtga agaggtacca gaacaatttg attcctctta tcttaaatgt cttcataagc gcttatttgg gaatatgttt gagtgggctg ggcagacacg tgacgaaatt tcatatttag aggatataaa aaaaaccaag ctgttaccaa tggaaaaatt gagctctgta agcagtaagg aaattcaaga acgttttaaa aaaattgaca aaattctttt cgagaagaat aatctgaaag atttatcgtg cgaagaattc tctcataatg tagcaagaat cttttctttg cttaatgata catacccttt tatcagtggg aatgaatgta cgcaaatggt gttttttgag cagcttgctc aagttgccaa ctataaactt gatttttcag ttataacaaa agaacgcatg aaacttgtca gcgatgctgc aaaaacttta aaaggcaaca taggcgatct agctcctata gaacactttt ttgaagatgt ttctgatcca aacaaagtgt gtatgctgaa agagtttatc aaaaataata aatgtcaaga taaaaatata aaaaatcaga tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BasaA.17330.a.B1.PS01725
PURIFICATION DATe: 1/10/2013
CONCENTRATION: 12.9mg/ml
OBSERVED MW: 72kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 42
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMF KRKIQEQAVS KYASDYAIPR SLMIKRRATV NSVISACMLE GYILAPKTLD ILKKYASGDY SLKQFNTQMD SSVKEVKVRK KYSTHGVITT NGMLVNKYGI TDAVALHKRS AHDIAKAIVN LSCEEVPEQF DSSYLKCLHK RLFGNMFEWA GQTRDEISYL EDIKKTKLLP MEKLSSVSSK EIQERFKKID KILFEKNNLK DLSCEEFSHN VARIFSLLND TYPFISGNEC TQMVFFEQLA QVANYKLDFS VITXNA
Validated NT Sequence
tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttta aaagaaagat acaggaacaa gcagtttcga aatatgcttc ggattatgct atacctcgaa gtctaatgat aaagcgtcgt gcaacagtta attcagtcat cagtgcgtgt atgcttgaag ggtatattct tgcccctaaa acgttagaca ttttaaagaa atatgcgagc ggagattatt cactaaagca gtttaacact cagatggatt cttctgtgaa agaagtaaag gtgcgaaaga aatattcaac ccatggtgtt attactacta atggaatgct ggtaaataaa tatggaataa cagatgcagt tgctcttcat aaaagaagtg cacatgatat agcaaaagca attgttaatc tatcttgtga agaggtacca gaacaatttg attcctctta tcttaaatgt cttcataagc gcttatttgg gaatatgttt gagtgggctg ggcagacacg tgacgaaatt tcatatttag aggatataaa aaaaaccaag ctgttaccaa tggaaaaatt gagctctgta agcagtaagg aaattcaaga acgttttaaa aaaattgaca aaattctttt cgagaagaat aatctgaaag atttatcgtg cgaagaattc tctcataatg tagcaagaat cttttctttg cttaatgata catacccttt tatcagtggg aatgaatgta cgcaaatggt gttttttgag cagcttgctc aagttgccaa ctataaactt gatttttcag ttataacaaa naacgcatga aacttgtcag cgatgctgcn aaanctttna anngnannnt nnnnatctag ctncnnnnga anactttttg aanangntnn nnatnnaann nnngnnnatg ctgannantt nnnnaaannn nnaangncan nnnaaannnt naaaaannnn annatnnnan nnccncnnan nnnntnnnnn nnnnnnnnnn nnnannnggt nnannnnnnn nnanncnnnn nnnnnnnnnc nnnnnnnnnn naannnnngn nnnnnnnnna nnnnnnnnnn gnannnnnnn tnnnnnnnnn nnnnnnnnnn nnnnnntnnn nnnna
Expressed Protein Sequence
MAHHHHHHMF KRKIQEQAVS KYASDYAIPR SLMIKRRATV NSVISACMLE GYILAPKTLD ILKKYASGDY SLKQFNTQMD SSVKEVKVRK KYSTHGVITT NGMLVNKYGI TDAVALHKRS AHDIAKAIVN LSCEEVPEQF DSSYLKCLHK RLFGNMFEWA GQTRDEISYL EDIKKTKLLP MEKLSSVSSK EIQERFKKID KILFEKNNLK DLSCEEFSHN VARIFSLLND TYPFISGNEC TQMVFFEQLA QVANYKLDFS VITKERMKLV SDAAKTLKGN IGDLAPIEHF FEDVSDPNKV CMLKEFIKNN KCQDKNIKNQ IIVAPRKDIV YGGICRNWSS NAILVETEDA YVVCHKDYLM AEELKTLKLG DKMSFTAVIP EDFGDILIPE GVLVSLAKEE IIKRVKKDSF VQRSLENVEY YSRVVYDDSE ILKKNMEFIN EFVCFDTQVA KRNTERLVDK IMESPQSIHV LAGVEIFGMK NSKRKKAEEC CGDLRLAINQ YIDNVIYARD AILQQYEAHN SFKRKAVAMP HKEIQDVLNL PENMRQEVLD TSCSLQQEIC DFVSDVMKRL SFSEYQALHD NNYEKLAQNL GVSVNKAEKI TKLFKAGEKV YLQAKNLKIN HSESMAITQR AS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTTA AAAGAAAGAT ACAGGAACAA GCAGTTTCGA AATATGCTTC GGATTATGCT ATACCTCGAA GTCTAATGAT AAAGCGTCGT GCAACAGTTA ATTCAGTCAT CAGTGCGTGT ATGCTTGAAG GGTATATTCT TGCCCCTAAA ACGTTAGACA TTTTAAAGAA ATATGCGAGC GGAGATTATT CACTAAAGCA GTTTAACACT CAGATGGATT CTTCTGTGAA AGAAGTAAAG GTGCGAAAGA AATATTCAAC CCATGGTGTT ATTACTACTA ATGGAATGCT GGTAAATAAA TATGGAATAA CAGATGCAGT TGCTCTTCAT AAAAGAAGTG CACATGATAT AGCAAAAGCA ATTGTTAATC TATCTTGTGA AGAGGTACCA GAACAATTTG ATTCCTCTTA TCTTAAATGT CTTCATAAGC GCTTATTTGG GAATATGTTT GAGTGGGCTG GGCAGACACG TGACGAAATT TCATATTTAG AGGATATAAA AAAAACCAAG CTGTTACCAA TGGAAAAATT GAGCTCTGTA AGCAGTAAGG AAATTCAAGA ACGTTTTAAA AAAATTGACA AAATTCTTTT CGAGAAGAAT AATCTGAAAG ATTTATCGTG CGAAGAATTC TCTCATAATG TAGCAAGAAT CTTTTCTTTG CTTAATGATA CATACCCTTT TATCAGTGGG AATGAATGTA CGCAAATGGT GTTTTTTGAG CAGCTTGCTC AAGTTGCCAA CTATAAACTT GATTTTTCAG TTATAACAAA AGAACGCATG AAACTTGTCA GCGATGCTGC AAAAACTTTA AAAGGCAACA TAGGCGATCT AGCTCCTATA GAACACTTTT TTGAAGATGT TTCTGATCCA AACAAAGTGT GTATGCTGAA AGAGTTTATC AAAAATAATA AATGTCAAGA TAAAAATATA AAAAATCAGA TTATTGTAGC TCCTCGTAAA GATATTGTTT ATGGTGGTAT CTGTAGAAAC TGGAGTTCAA ATGCCATCCT AGTCGAAACG GAAGATGCGT ACGTCGTATG TCATAAAGAT TATTTAATGG CAGAAGAATT AAAAACATTA AAATTAGGTG ATAAAATGTC TTTTACAGCT GTAATACCTG AGGATTTTGG AGACATTCTC ATTCCAGAAG GAGTATTAGT TTCTTTGGCG AAAGAAGAAA TTATTAAAAG AGTTAAAAAA GATTCTTTTG TTCAAAGAAG CTTGGAAAAT GTTGAGTATT ATTCAAGAGT TGTTTATGAT GATTCAGAAA TATTGAAAAA AAATATGGAA TTTATTAATG AATTTGTATG TTTTGATACG CAGGTTGCGA AGCGGAATAC GGAGAGGCTT GTGGATAAAA TTATGGAATC TCCTCAATCT ATTCATGTGC TTGCAGGTGT TGAGATATTT GGTATGAAAA ATTCAAAGCG CAAGAAAGCT GAAGAATGTT GTGGTGACCT TAGGCTTGCA ATTAATCAAT ATATAGATAA TGTAATATAC GCTAGAGATG CAATTTTACA ACAATACGAA GCACACAATA GTTTTAAAAG GAAAGCTGTA GCAATGCCCC ATAAGGAAAT ACAAGATGTT TTAAATTTGC CAGAAAATAT GCGGCAAGAA GTATTAGACA CTTCTTGTTC ATTACAGCAG GAGATTTGTG ATTTTGTATC TGACGTCATG AAGCGTCTTT CATTTAGCGA GTATCAAGCA CTTCATGATA ATAATTATGA AAAGCTCGCT CAAAATTTGG GCGTGTCAGT GAATAAAGCA GAGAAAATTA CAAAACTTTT TAAAGCGGGT GAGAAAGTGT ATCTGCAAGC TAAAAATCTC AAAATTAATC ACTCAGAAAG TATGGCTATA ACCCAGAGAG CCAGTTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT