SSGCID: Target Page

BasaA.01664.a

Two-component sensor kinase BatS (EC 2.7.3.-) (BatR-BatS two-component system)

CENTER ID:	BasaA.01664.a
ORGANISM:	Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BasaA.01664.a.B2.GE35931	kinase domain		297	558
BasaA.01664.a.B3.GE36606	periplasmic domain		32	243

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BasaA.01664.a.B2.PS01669	kinase domain		297	558

External Resources

RESOURCE	REFERENCE ID
UniProt:	E6YNY0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRQLFFSSLT RRIVILNLAA LGILITSILY LNQFRNGLIE IKIESLRTQG KIIAGAIAAS ATIETNSILI NPQKLLELHA GESTTPMLQS TDTWDFPINS EQIAPLLRRL IAPKTTRARI YDRDATLLLD SRVIYSSGEV FTYDLPPIQT KQNLWEYLKS LFFNTLYSNG IAVDRRQAKD NEALHPEVYL ALNGSSATAK RRNRQGQLIV SVAVPVQRYR AVVGALLLST TGSDIDDIVE SERLGILKIF AVVGSVFLVL SLFLAHTIAH PLSKLSSSAN RVRHGRSKRI KIPDFSKRED EIGHLSTSIR DMTNALYTRI EAIERFAADV SHELKNPLTS LRSAVETLPL AKNQAQQTQL LEIIQHDVHR LDRLITDISD ASRLDAELVR ETAKIVDMSS LLKSLIQAIR EVHCNKQDID INFNIIPRTN GKPYLIVGHE LRLGQVVSNL IENARSFVPR KNGKICITMK NNTSTLILTV EDNGPGIRSE NIERIFERFY TDRSSKNAFG QHSGLGLAIS RQIIEAHNGT LMAENIVDPT SKKEKIGARF IITLPLAK

NT Sequence

ttgagacaat tattcttttc tagtctcaca cgccgtattg ttatcttaaa tcttgctgct cttggtattt taattacaag tattttatat cttaatcaat tccgcaatgg tttgattgaa ataaaaattg aaagtttgcg cacccaagga aaaattattg ctggtgccat tgctgcctct gcgacaatag aaacaaattc tattttaatt aaccctcaaa aacttttgga attacacgct ggggaaagta ctacaccaat gcttcaatca actgatactt gggattttcc cattaattcc gaacaaattg ctccactttt acgacgtctt attgcaccaa aaacgacaag agcacggata tatgatcgtg acgcaacttt gcttcttgat tcacgtgtta tttattcgag tggtgaagtt tttacctatg atttacctcc tatccaaaca aaacaaaatt tgtgggagta tttaaaatct ttgtttttca atacactata cagcaacggt attgctgtcg atagaagaca agcaaaagat aatgaagccc ttcaccctga agtttaccta gcattaaatg gctcttctgc cactgctaaa cgacgtaaca gacaaggtca attaattgtt tcggttgctg tacctgttca acgttatcgt gcagtagttg gtgctctttt gctttcaact actggttcag acattgatga tattgtagaa agtgaaagat taggaatttt aaaaatcttt gccgttgttg gaagcgtttt tttagtcctt tctctatttt tagctcatac tattgcgcat ccactaagta aattatcttc ctccgctaat cgtgtgcgtc atggtcgcag taagcgaata aaaattcctg atttttcaaa acgcgaagat gaaattggtc atctttctac ctctattcgt gatatgacca acgctcttta tacacgtatt gaagctattg aacgttttgc agccgacgta agtcatgaat taaagaaccc tctcacctcg ctgcgaagtg ctgttgagac attaccacta gctaaaaatc aagcacaaca gacacaatta cttgaaatta tccaacatga tgtgcatcgt cttgatcgtt taattaccga tatttctgat gcatcgcggt tagatgcaga acttgttcgc gaaactgcta aaatagttga tatgagttca ttgttaaaaa gccttattca agctattcgt gaagtccatt gcaacaaaca agatattgat attaatttta atattatccc acggactaat ggtaaacctt atttaattgt aggacatgaa ttacgtttag gtcaggtagt ttccaatctc attgaaaatg cacgttcttt tgttcctcgt aaaaatggta aaatttgtat tacgatgaaa aataacactt caactctcat tttaactgtc gaagacaatg gccctggtat tcgttcagag aatattgaac gcatttttga acgtttctat actgatcgat caagcaaaaa tgcttttgga caacactcag gacttggcct tgcaattagt cggcaaatca ttgaagctca taatggaact ctcatggctg aaaatattgt tgatcccaca tctaaaaaag aaaaaattgg tgctcgattt attatcactc ttccacttgc caaataa

Details for BasaA.01664.a.B2.GE35931

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK REDEIGHLST SIRDMTNALY TRIEAIERFA ADVSHELKNP LTSLRSAVET LPLAKNQAQQ TQLLEIIQHD VHRLDRLITD ISDASRLDAE LVRETAKIVD MSSLLKSLIQ AIREVHCNKQ DIDINFNIIP RTNGKPYLIV GHELRLGQVV SNLIENARSF VPRKNGKICI TMKNNTSTLI LTVEDNGPGI RSENIERIFE RFYTDRSSKN AFGQHSGLGL AISRQIIEAH NGTLMAENIV DPTSKKEKIG ARFIITLPLA K

Validated NT Sequence

gatcctatct tactcactta tttggcaagt ggaagagtga taataaatcg agcaccaatt ttttcttttt tagatgtggg atcaacaata ttttcagcca tgagagttcc attatgagct tcaatgattt gccgactaat tgcaaggcca agtcctgagt gttgtccaaa agcatttttg cttgatcgat cagtatagaa acgttcaaaa atgcgttcaa tattctctga acgaatacca gggccattgt cttcgacagt taaaatgaga gttgaagtgt tatttttcat cgtaatacaa attttaccat ttttacgagg aacaaaagaa cgtgcatttt caatgagatt ggaaactacc tgacctaaac gtaattcatg tcctacaatt aaataaggtt taccattagt ccgtgggata atattaaaat taatatcaat atcttgtttg ttgcaatgga cttcacgaat agcttgaata aggcttttta acaatgaact catatcaact attttagcag tttcgcgaac aagttctgca tctaaccgcg atgcatcaga aatatcggta attaaacgat caagacgatg cacatcatgt tggataattt caagtaattg tgtctgttgt gcttgatttt tagctagtgg taatgtctca acagcacttc gcagcgaggt gagagggttc tttaattcat gacttacgtc ggctgcaaaa cgttcaatag cttcaatacg tgtataaaga gcgttggtca tatcacgaat agaggtagaa agatgaccaa tttcatcttc gcgtttcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac gcgaagatga aattggtcat ctttctacct ctattcgtga tatgaccaac gctctttata cacgtattga agctattgaa cgttttgcag ccgacgtaag tcatgaatta aagaaccctc tcacctcgct gcgaagtgct gttgagacat taccactagc taaaaatcaa gcacaacaga cacaattact tgaaattatc caacatgatg tgcatcgtct tgatcgttta attaccgata tttctgatgc atcgcggtta gatgcagaac ttgttcgcga aactgctaaa atagttgata tgagttcatt gttaaaaagc cttattcaag ctattcgtga agtccattgc aacaaacaag atattgatat taattttaat attatcccac ggactaatgg taaaccttat ttaattgtag gacatgaatt acgtttaggt caggtagttt ccaatctcat tgaaaatgca cgttcttttg ttcctcgtaa aaatggtaaa atttgtatta cgatgaaaaa taacacttca actctcattt taactgtcga agacaatggc cctggtattc gttcagagaa tattgaacgc atttttgaac gtttctatac tgatcgatca agcaaaaatg cttttggaca acactcagga cttggccttg caattagtcg gcaaatcatt gaagctcata atggaactct catggctgaa aatattgttg atcccacatc taaaaaagaa aaaattggtg ctcgatttat tatcactctt ccacttgcca aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for BasaA.01664.a.B3.GE36606

HARVESTED ON:	2/8/2013
SEQUENCED ON:	2/14/2013
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN QFRNGLIEIK IESLRTQGKI IAGAIAASAT IETNSILINP QKLLELHAGE STTPMLQSTD TWDFPINSEQ IAPLLRRLIA PKTTRARIYD RDATLLLDSR VIYSSGEVFT YDLPPIQTKQ NLWEYLKSLF FNTLYSNGIA VDRRQAKDNE ALHPEVYLAL NGSSATAKRR NRQGQLIVSV AVPVQRYRAV VGALLLSTTG SDIDDIVESE R

Validated NT Sequence

aattttgttt aactttaaga aggaganata ccatggctca ccaccaccac caccatatga atcaattccg caatggtttg attgaaataa aaattgaaag tttgcgcacc caaggaaaaa ttattgctgg tgccattgct gcctctgcga caatagaaac aaattctatt ttaattaacc ctcaaaaact tttggaatta cacgctgggg aaagtactac accaatgctt caatcaactg atacttggga ttttcccatt aattccgaac aaattgctcc acttttacga cgtcttattg caccaaaaac gacaagagca cggatatatg atcgtgacgc aactttgctt cttgattcac gtgttattta ttcgagtggt gaagttttta cctatgattt acctcctatc caaacaaaac aaaatttgtg ggagtattta aaatctttgt ttttcaatac actatacagc aacggtattg ctgtcgatag aagacaagca aaagataatg aagcccttca ccctgaagtt tacctagcat taaatggctc ttctgccact gctaaacgac gtaacagaca aggtcaatta attgtttcgg ttgctgtacc tgttcaacgt tatcgtgcag tagttggtgc tcttttgctt tcaactactg gttcagacat tgatgatatt gtagaaagtg aaagataagt gagtaagata ggatccggct gctaacannc cccnnaagna nnnnncnnnn annnnnnnnn nnnnnngnnn nnnnnncccn nngnnnnnnn nnnnnnnnna tgnnnnnncn attntnnttn nnncttnnnc nncnnncnnc nnnnnnnnnn natttnnnnn nnncnnnnnn cgnnnctnnn ntgncanann acnttcanta nngnnnnnnn nnnncgnnna ntnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc aattccgcaa tggtttgatt gaaataaaaa ttgaaagttt gcgcacccaa ggaaaaatta ttgctggtgc cattgctgcc tctgcgacaa tagaaacaaa ttctatttta attaaccctc aaaaactttt ggaattacac gctggggaaa gtactacacc aatgcttcaa tcaactgata cttgggattt tcccattaat tccgaacaaa ttgctccact tttacgacgt cttattgcac caaaaacgac aagagcacgg atatatgatc gtgacgcaac tttgcttctt gattcacgtg ttatttattc gagtggtgaa gtttttacct atgatttacc tcctatccaa acaaaacaaa atttgtggga gtatttaaaa tctttgtttt tcaatacact atacagcaac ggtattgctg tcgatagaag acaagcaaaa gataatgaag cccttcaccc tgaagtttac ctagcattaa atggctcttc tgccactgct aaacgacgta acagacaagg tcaattaatt gtttcggttg ctgtacctgt tcaacgttat cgtgcagtag ttggtgctct tttgctttca actactggtt cagacattga tgatattgta gaaagtgaaa gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for BasaA.01664.a.B2.PS01669

PURIFICATION DATe:	11/1/2012
CONCENTRATION:	42.9mg/ml
OBSERVED MW:	29kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAC GCGAAGATGA AATTGGTCAT CTTTCTACCT CTATTCGTGA TATGACCAAC GCTCTTTATA CACGTATTGA AGCTATTGAA CGTTTTGCAG CCGACGTAAG TCATGAATTA AAGAACCCTC TCACCTCGCT GCGAAGTGCT GTTGAGACAT TACCACTAGC TAAAAATCAA GCACAACAGA CACAATTACT TGAAATTATC CAACATGATG TGCATCGTCT TGATCGTTTA ATTACCGATA TTTCTGATGC ATCGCGGTTA GATGCAGAAC TTGTTCGCGA AACTGCTAAA ATAGTTGATA TGAGTTCATT GTTAAAAAGC CTTATTCAAG CTATTCGTGA AGTCCATTGC AACAAACAAG ATATTGATAT TAATTTTAAT ATTATCCCAC GGACTAATGG TAAACCTTAT TTAATTGTAG GACATGAATT ACGTTTAGGT CAGGTAGTTT CCAATCTCAT TGAAAATGCA CGTTCTTTTG TTCCTCGTAA AAATGGTAAA ATTTGTATTA CGATGAAAAA TAACACTTCA ACTCTCATTT TAACTGTCGA AGACAATGGC CCTGGTATTC GTTCAGAGAA TATTGAACGC ATTTTTGAAC GTTTCTATAC TGATCGATCA AGCAAAAATG CTTTTGGACA ACACTCAGGA CTTGGCCTTG CAATTAGTCG GCAAATCATT GAAGCTCATA ATGGAACTCT CATGGCTGAA AATATTGTTG ATCCCACATC TAAAAAAGAA AAAATTGGTG CTCGATTTAT TATCACTCTT CCACTTGCCA AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT