SSGCID: Target Page

BasaA.00806.a

Transcriptional regulator BatR (BatR-BatS two-component system)

CENTER ID:	BasaA.00806.a
ORGANISM:	Bartonella sp. AR 15-3
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BasaA.00806.a.B1.GE35662	full length		1	240

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	E6YNY1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKDTPTVTQT IVLVDDDCNI LTSLSFALET EGYRVESYTD GSAALKNLTI HPPHLAIFDI KMPRMDGMEL LRRLRQKSDI PVIFLTSKDD EIDELFGLKM GADDFITKPF SQRLLIERVK AVLRRSHARN QPLPIGTTST SSLKRGDLIM DQERHICTWK NKPVILTVTE FLILQTLAQR PGVVKSRDAL MDAVYNDQIY VDDRTIDSHI KRLRKKFKQV DNDFAMIETL YGVGYRFHEV

NT Sequence

atgaaggata ctccaaccgt cacccaaacg attgtactgg ttgacgatga ttgtaatatt ttaacatctt tatcttttgc ccttgaaaca gaagggtatc gggttgaaag ttatacagat ggatcagctg cacttaaaaa tttaacaatt caccctccac acttagctat ttttgatatt aaaatgccac gaatggatgg tatggagctt ttgcgtcgtt tacgtcaaaa atctgacatt ccagttattt ttctaacctc taaagacgat gaaattgatg aactgtttgg tctcaaaatg ggagctgatg attttatcac aaaacctttt tctcaacgtc ttcttattga gcgagtaaaa gctgttttac gtcgttccca tgcccgcaac cagccacttc caataggaac aacatcaact tcttctctca aacgtggtga tcttattatg gatcaagagc gccatatctg tacatggaaa aacaaacctg ttatattaac agttacagag tttttgattc tgcaaacttt agcgcagcga ccaggagttg ttaaaagtcg tgatgcttta atggatgctg tctataacga tcaaatttat gtagatgatc gtactattga tagtcacatt aagcgtttgc gtaaaaaatt taaacaagta gataatgatt ttgcaatgat tgaaacgctt tatggtgtag gttatcgttt tcatgaagta tga

Details for BasaA.00806.a.B1.GE35662

HARVESTED ON:	7/10/2012
SEQUENCED ON:	7/30/2012
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK DTPTVTQTIV LVDDDCNILT SLSFALETEG YRVESYTDGS AALKNLTIHP PHLAIFDIKM PRMDGMELLR RLRQKSDIPV IFLTSKDDEI DELFGLKMGA DDFITKPFSQ RLLIERVKAV LRRSHARNQP LPIGTTSTSS LKRGDLIMDQ ERHICTWKNK PVILTVTEFL ILQTLAQRPG VVKSRDALMD AVYNDQIYVD DRTIDSHIKR LRKKFKQVDN DFAMIETLYG VGYRFHEV

Validated NT Sequence

atcttactca cttatacttc atgaaaacga taacctacac cataaagcgt ttcaatcatt gcaaaatcat tatctacttg tttaaatttt ttacgcaaac gcttaatgtg actatcaata gtacgatcat ctacataaat ttgatcgtta tagacagcat ccattaaagc atcacgactt ttaacaactc ctggtcgctg cgctaaagtt tgcagaatca aaaactctgt aactgttaat ataacaggtt tgtttttcca tgtacagata tggcgctctt gatccataat aagatcacca cgtttgagag aagaagttga tgttgttcct attggaagtg gctggttgcg ggcatgggaa cgacgtaaaa cagcttttac tcgctcaata agaagacgtt gagaaaaagg ttttgtgata aaatcatcag ctcccatttt gagaccaaac agttcatcaa tttcatcgtc tttagaggtt agaaaaataa ctggaatgtc agatttttga cgtaaacgac gcaaaagctc cataccatcc attcgtggca ttttaatatc aaaaatagct aagtgtggag ggtgaattgt taaattttta agtgcagctg atccatctgt ataactttca acccgatacc cttctgtttc aagggcaaaa gataaagatg ttaaaatatt acaatcatcg tcaaccagta caatcgtttg ggtgacggtt ggagtatcct tcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagg atactccaac cgtcacccaa acgattgtac tggttgacga tgattgtaat attttaacat ctttatcttt tgcccttgaa acagaagggt atcgggttga aagttataca gatggatcag ctgcacttaa aaatttaaca attcaccctc cacacttagc tatttttgat attaaaatgc cacgaatgga tggtatggag cttttgcgtc gtttacgtca aaaatctgac attccagtta tttttctaac ctctaaagac gatgaaattg atgaactgtt tggtctcaaa atgggagctg atgattttat cacaaaacct ttttctcaac gtcttcttat tgagcgagta aaagctgttt tacgtcgttc ccatgcccgc aaccagccac ttccaatagg aacaacatca acttcttctc tcaaacgtgg tgatcttatt atggatcaag agcgccatat ctgtacatgg aaaaacaaac ctgttatatt aacagttaca gagtttttga ttctgcaaac tttagcgcag cgaccaggag ttgttaaaag tcgtgatgct ttaatggatg ctgtctataa cgatcaaatt tatgtagatg atcgtactat tgatagtcac attaagcgtt tgcgtaaaaa atttaaacaa gtagataatg attttgcaat gattgaaacg ctttatggtg taggttatcg ttttcatgaa gtatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat