SSGCID: Target Page

BaroA.17330.j

Bep7 protein (VirB-translocated Bartonella effector protein)

CENTER ID:	BaroA.17330.j
ORGANISM:	Bartonella rochalimae ATCC BAA-1498
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaroA.17330.j.B3.GE36605	FIC and truncated OB-Fold		8	294
BaroA.17330.j.B4.GE37275	BID		305	441

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|685782.3.peg.1333
UniProt:	E6YLF1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPSFGCDYTY PNSDVLINKY GIKNSQALHE KSAYHSEQAI IRLRQEALPN KFNSSYLKHI HKCLFEDTFE WAGCTRDLTF KFKNGVTANI SKMQIPNSDA FFKESEAVSK SLHKFDQVLS KKNNLQGLSR EEFVNEATKL FSFLNYIHPF RDGNGLAQRV FFERLAEAAG HKLDFSVVTQ KRMIHACNDA IPVEGNVNYE TMQHLFEDIS NPEKRRILKD YLNAVSNNKH ACNFLDDQII ITPREGVTYT GFYKVSNADS IIVETVDFYM VCCKDYFTPE QLKALKPNDE ITFTVSINKD LDQILIPAEK IPPLTSEKIL QKIENHIKLQ NNRKEIENLS QIVYGNSKAL DPSIDIINKG REVSKKIPKQ ILNDPESIAK LAGFKILGIR APGRRIAERN LAPLSQKIKN YMSAVEVTKN IIIRNHVIEQ KRLLQEVRLP SKLMQDVLSV VQNEKSQALN VENCVKIQKE LISFLTKFES RLSETESQAF REGDYKQIAE SIGMSEHKAK ILISTVNKAK KMYRQLQQLK VNKSIQMGVA V

NT Sequence

ttgccttctt ttggttgtga ttacacttat cctaacagtg atgtattaat caataaatat ggaataaaga attctcaagc tcttcatgaa aagagcgcgt atcattcaga gcaagcgatt attcgtttgc gtcaagaagc tctgccaaat aagtttaatt cctcttattt gaaacatatt cataaatgtt tatttgaaga tacgttcgaa tgggctggct gtacacgtga tcttacattt aaatttaaaa atggcgtaac tgctaacatc tcaaagatgc aaataccaaa ttcagatgct ttttttaaag agagtgaagc agtttcaaaa agtttacata aatttgatca agtgctttcg aaaaaaaata atttacaagg cttatcgcgt gaagaatttg ttaatgaagc aacgaagctt ttttcctttc tcaactatat acatccattt agagatggga atgggcttgc acaacgagtc ttttttgaaa gacttgctga agctgcaggc cataaacttg atttttcagt tgtgactcaa aaacgcatga tacatgcttg taatgatgcg atacctgtag aaggtaatgt aaattatgaa acgatgcaac atttgttcga agatatctct aatccagaaa aaagacgcat tttgaaggat tatttaaatg ctgtgtctaa taataagcat gcatgtaatt ttttggatga tcaaattatc ataacaccac gtgaaggtgt gacttataca ggtttttata aggttagtaa tgcggattct attatagttg aaacagtaga cttttatatg gtatgctgca aagattattt tacaccagaa cagcttaagg cattaaaacc taatgatgag attactttta cagtttcaat aaataaagat ttagatcaaa ttctcatccc agcagaaaaa atacctcctt taacgtcaga aaaaattctt caaaaaattg agaatcatat taaacttcaa aacaatcgaa aagaaattga gaatttatca caaattgttt atggaaattc aaaagcgtta gatccaagta ttgatataat taataaagga cgagaagtaa gtaaaaaaat tcctaaacaa attttaaatg atcctgaatc tattgctaag ctagctggtt ttaaaatatt gggtataaga gctccaggac gcagaatagc tgaaaggaat cttgcaccgc ttagtcaaaa aattaaaaat tatatgtctg ctgtagaagt tactaaaaat ataatcatta gaaatcatgt aatagaacaa aaacgccttt tacaagaagt aagactgcct agtaagctaa tgcaagatgt ccttagcgtg gttcaaaatg agaaatcaca agccttaaat gtagaaaatt gtgttaagat ccaaaaagag cttatttcct ttttgactaa atttgaatct cgtctttcag agacagaatc tcaagcattc agagaaggtg attataaaca aatcgctgaa agtattggta tgtcagaaca taaagcaaaa atacttataa gcactgttaa taaagcaaaa aaaatgtatc gacaacttca acaattgaaa gtgaataagt ctatacagat gggcgtagcc gtttaa

Details for BaroA.17330.j.B3.GE36605

HARVESTED ON:	2/8/2013
SEQUENCED ON:	2/14/2013
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMY TYPNSDVLIN KYGIKNSQAL HEKSAYHSEQ AIIRLRQEAL PNKFNSSYLK HIHKCLFEDT FEWAGCTRDL TFKFKNGVTA NISKMQIPNS DAFFKESEAV SKSLHKFDQV LSKKNNLQGL SREEFVNEAT KLFSFLNYIH PFRDGNGLAQ RVFFERLAEA AGHKLDFSVV TQKRMIHACN DAIPVEGNVN YETMQHLFED ISNPEKRRIL KXYLNAVSNN KHACNFLDDQ IIITPREGVT YTGFYKXSNA XSIIVETVDF YMVCCKDYFT PEQLKAXKPN DEITFT

Validated NT Sequence

aattttgntt anctttaaga aggaganata ccatggctca ccaccaccac caccatatgt acacttatcc taacagtgat gtattaatca ataaatatgg aataaagaat tctcaagctc ttcatgaaaa gagcgcgtat cattcagagc aagcgattat tcgtttgcgt caagaagctc tgccaaataa gtttaattcc tcttatttga aacatattca taaatgttta tttgaagata cgttcgaatg ggctggctgt acacgtgatc ttacatttaa atttaaaaat ggcgtaactg ctaacatctc aaagatgcaa ataccaaatt cagatgcttt ttttaaagag agtgaagcag tttcaaaaag tttacataaa tttgatcaag tgctttcgaa aaaaaataat ttacaaggct tatcgcgtga agaatttgtt aatgaagcaa cgaagctttt ttcctttctc aactatatac atccatttag agatgggaat gggcttgcac aacgagtctt ttttgaaaga cttgctgaag ctgcaggcca taaacttgat ttttcagttg tgactcaaaa acgcatgata catgcttgta atgatgcgat acctgtagaa ggtaatgtaa attatgaaac gatgcaacat ttgttcgaag atatctctaa tccagaaaaa agacgcattt tgaagganta tttaaatgct gtgtctaata ataagcatgc atgtaatttt ttggatgatc aaattatcat aacaccacgt gaaggtgtga cttatacagg tttttataag gntagtaatg cggantctat tatagttgaa acagtagact tttatatggt atgctgcaaa gattatttta caccagaaca gcttaaggca ntaaaaccta atgatgagat tacttttaca taagtgagta anannnnnnn nnnnnnnnnc cccnnnncnn nnnnnnnnnn nnnnnnnnna aaaaaaaaaa annncnntnn cncnctctnn ncncntnnnn nttntttttt

Expected Protein Sequence

MAHHHHHHMY TYPNSDVLIN KYGIKNSQAL HEKSAYHSEQ AIIRLRQEAL PNKFNSSYLK HIHKCLFEDT FEWAGCTRDL TFKFKNGVTA NISKMQIPNS DAFFKESEAV SKSLHKFDQV LSKKNNLQGL SREEFVNEAT KLFSFLNYIH PFRDGNGLAQ RVFFERLAEA AGHKLDFSVV TQKRMIHACN DAIPVEGNVN YETMQHLFED ISNPEKRRIL KDYLNAVSNN KHACNFLDDQ IIITPREGVT YTGFYKVSNA DSIIVETVDF YMVCCKDYFT PEQLKALKPN DEITFT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtaca cttatcctaa cagtgatgta ttaatcaata aatatggaat aaagaattct caagctcttc atgaaaagag cgcgtatcat tcagagcaag cgattattcg tttgcgtcaa gaagctctgc caaataagtt taattcctct tatttgaaac atattcataa atgtttattt gaagatacgt tcgaatgggc tggctgtaca cgtgatctta catttaaatt taaaaatggc gtaactgcta acatctcaaa gatgcaaata ccaaattcag atgctttttt taaagagagt gaagcagttt caaaaagttt acataaattt gatcaagtgc tttcgaaaaa aaataattta caaggcttat cgcgtgaaga atttgttaat gaagcaacga agcttttttc ctttctcaac tatatacatc catttagaga tgggaatggg cttgcacaac gagtcttttt tgaaagactt gctgaagctg caggccataa acttgatttt tcagttgtga ctcaaaaacg catgatacat gcttgtaatg atgcgatacc tgtagaaggt aatgtaaatt atgaaacgat gcaacatttg ttcgaagata tctctaatcc agaaaaaaga cgcattttga aggattattt aaatgctgtg tctaataata agcatgcatg taattttttg gatgatcaaa ttatcataac accacgtgaa ggtgtgactt atacaggttt ttataaggtt agtaatgcgg attctattat agttgaaaca gtagactttt atatggtatg ctgcaaagat tattttacac cagaacagct taaggcatta aaacctaatg atgagattac ttttacatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BaroA.17330.j.B4.GE37275

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	17kDa
OBSERVED MW:	17kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHLI PAEKIPPLTS EKILQKIENH IKLQNNRKEI ENLSQIVYGN SKALDPSIDI INKGREVSKK IPKQILNDPE SIAKLAGFKI LGIRAPGRRI AERNLAPLSQ KIKNYMSAVE VTKNIIIRNH VIEQKRLLQE VRLPS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctcatcc cagcagaaaa aatacctcct ttaacgtcag aaaaaattct tcaaaaaatt gagaatcata ttaaacttca aaacaatcga aaagaaattg agaatttatc acaaattgtt tatggaaatt caaaagcgtt agatccaagt attgatataa ttaataaagg acgagaagta agtaaaaaaa ttcctaaaca aattttaaat gatcctgaat ctattgctaa gctagctggt tttaaaatat tgggtataag agctccagga cgcagaatag ctgaaaggaa tcttgcaccg cttagtcaaa aaattaaaaa ttatatgtct gctgtagaag ttactaaaaa tataatcatt agaaatcatg taatagaaca aaaacgcctt ttacaagaag taagactgcc tagttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat