BaroA.17330.i

Bep10 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaroA.17330.i
ORGANISM: Bartonella rochalimae ATCC BAA-1498
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaroA.17330.i.B2.GE36602 FIC 22 257
BaroA.17330.i.B3.GE36603 FIC and truncated OB-Fold 22 313
BaroA.17330.i.B4.GE37274 BID 322 458
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|685782.3.peg.560
RefSeq: WP_035006229.1
UniProt: E6YMR3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFIKDSQTFK NAEKSLLKNS INYTYAESGD PYTLINKLGI QDHQQLKIKC AHKVTKELVN VHQEPIPQKV DQLYLKKLHK RLFGAIFDWA GETRDNPIKM SNGKIATMLV NDKNSPFASN EKLSKCLNEL DAKLAQRAQR NEAGNTSQKK VSDKNEPLSE DIATIYALLD HARPFVEGNG IVQRLFVNKL SQAENQKLDF SVVTQKRMED AIVSAANDNL QPMQHLFEDA SNPENIKVLK TAINRMTPEE AQEIARGIIT TPISGKIYTG VYESRQLDTI VIRTSNSLIV CNKSVIPREQ FQKLKLGDKI SFKALSKLSD VLIPGKELRN LTDFKIMERT VCNSYVLHQR KEIERLAKVV FGNREKLTNH LERIHDNPNS AKMLSETILH NPKSIAKLAG FGKYGINSPK RNKAEKSLSA LGEAILRYGS DLKTSKEEVL REHRAEQYRL AKEVSLPGEK IQRYLDMSKE ERESALKIEG NVTICKEISA FLKQINSRLS VSERKMVNSG SYESLAESIG IPEDKAKQVI DTIQQIKVLQ KQAMLIREPR QQTIAIAS
NT Sequence
atgtttataa aagattctca aactttcaaa aacgcagaaa aatcattact gaaaaacagt attaactaca cctatgctga atctggagat ccctatacgt taataaataa acttgggata caagatcacc agcaacttaa gataaagtgt gcgcataaag tgacgaaaga acttgttaat gtgcatcaag aaccgatacc tcaaaaagtt gatcagcttt acctaaaaaa acttcacaaa cgcttatttg gagcgatatt tgattgggct ggagaaaccc gtgataatcc gattaaaatg tcaaatggta aaattgctac catgttagtg aatgataaaa attctccctt tgcatctaat gaaaagctct caaaatgctt aaatgaactt gatgcaaagc ttgctcaaag agctcaaaga aacgaagcag gaaatacctc acaaaaaaaa gtttcagata aaaatgaacc gcttagtgaa gatatagcaa ctatttatgc actacttgat catgcacgtc ctttcgtaga gggcaatgga attgtacaac ggttatttgt gaataaactc tctcaagctg agaatcaaaa gcttgatttt tcagttgtga cacaaaagcg tatggaagat gcaattgtta gtgcagcaaa tgacaattta cagcctatgc aacatctgtt tgaagatgct tctaatccag aaaacatcaa ggttttgaaa acagctataa atagaatgac acctgaagaa gcgcaagaga ttgcaagagg aattattaca acaccaatct caggaaaaat ctatacaggt gtctatgaaa gtcgtcaatt agatactatt gtgataagaa catcgaattc tttgattgta tgcaataaat ctgttattcc aagggagcag ttccaaaaat taaagctagg tgataaaatc agctttaaag ctcttagtaa gcttagtgat gttttaatcc caggaaaaga attacgcaat ttaacagact ttaaaattat ggaaaggacc gtatgtaatt cttatgttct acatcaaaga aaagaaattg aacgtttggc aaaagttgta tttggaaacc gtgaaaaatt aaccaatcat ctagaacgaa ttcatgataa tccaaattca gcgaaaatgc tttctgaaac tatcttacac aaccctaaat ctattgctaa gcttgcaggt tttggaaaat atggaataaa cagtccaaaa cgtaataaag ctgaaaaaag tctttctgca ctcggtgaag caattttgag atatgggagt gatttgaaaa cttctaaaga agaagttttg agagaacata gagcagaaca ataccgtctt gcaaaagagg taagtttacc tggcgaaaag atacagcgtt atcttgatat gtcaaaagaa gaacgtgaaa gtgctttaaa aatagaagga aatgttacta tttgcaaaga gattagcgct tttttaaagc aaattaattc tcgtctttcg gtaagtgaac gtaaaatggt taacagtggt agttatgaat ctttggctga aagcattggt attccagaag acaaagcaaa acaagttatt gataccattc aacaaatcaa agttttacaa aagcaagcaa tgttaattag agaacctcgt caacaaacaa tagctatagc cagctaa
Details for BaroA.17330.i.B2.GE36602
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN YTYAESGDPY TLINKLGIQD HQQLKIKCAH KVTKELVNVH QEPIPQKVDQ LYLKKLHKRL FGAIFDWAGE TRDNPIKMSN GKIATMLVND KNSPFASNEK LSKCLNELDA KLAQRAQRNE AGNTSQKKVS DKNEPLSEDI ATIYALLDHA RPFVEGNGIV QRLFVNKLSQ AENQKLDFSV VTQKRMEDAI VSAANDNLQP MQHLFEDASN PENIKVLKTA INRMTPEEAQ EIARG
Validated NT Sequence
ttgtttanct ttaagaagga gatataccat ggctcaccac caccaccacc atatgaacta cacctatgct gaatctggag atccctatac gttaataaat aaacttggga tacaagatca ccagcaactt aagataaagt gtgcgcataa agtgacgaaa gaacttgtta atgtgcatca agaaccgata cctcaaaaag ttgatcagct ttacctaaaa aaacttcaca aacgcttatt tggagcgata tttgattggg ctggagaaac ccgtgataat ccgattaaaa tgtcaaatgg taaaattgct accatgttag tgaatgataa aaattctccc tttgcatcta atgaaaagct ctcaaaatgc ttaaatgaac ttgatgcaaa gcttgctcaa agagctcaaa gaaacgaagc aggaaatacc tcacaaaaaa aagtttcaga taaaaatgaa ccgcttagtg aagatatagc aactatttat gcactacttg atcatgcacg tcctttcgta gagggcaatg gaattgtaca acggttattt gtgaataaac tctctcaagc tgagaatcaa aagcttgatt tttcagttgt gacacaaaag cgtatggaag atgcaattgt tagtgcagca aatgacaatt tacagcctat gcaacatctg tttgaagatg cttctaatcc agaaaacatc aaggttttga aaacagctat aaatagaatg acacctgaag aagcgcaaga gattgcaaga ggataagtga gtaaganagg atccggctgc nnnnnncccc cnnaanngan nnnnnnnnnn annnnnnnaa nnnnnnnnnn nnnnnnnnnn nncnnnncnc nnnnnnnnnc nnnnnnnnnn nnnnnnntnn nnnnnnnnnn nnnnnnncnn nnnnnnncnn nnnnnnnnnn nnnnttt
Expected Protein Sequence
MAHHHHHHMN YTYAESGDPY TLINKLGIQD HQQLKIKCAH KVTKELVNVH QEPIPQKVDQ LYLKKLHKRL FGAIFDWAGE TRDNPIKMSN GKIATMLVND KNSPFASNEK LSKCLNELDA KLAQRAQRNE AGNTSQKKVS DKNEPLSEDI ATIYALLDHA RPFVEGNGIV QRLFVNKLSQ AENQKLDFSV VTQKRMEDAI VSAANDNLQP MQHLFEDASN PENIKVLKTA INRMTPEEAQ EIARG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaact acacctatgc tgaatctgga gatccctata cgttaataaa taaacttggg atacaagatc accagcaact taagataaag tgtgcgcata aagtgacgaa agaacttgtt aatgtgcatc aagaaccgat acctcaaaaa gttgatcagc tttacctaaa aaaacttcac aaacgcttat ttggagcgat atttgattgg gctggagaaa cccgtgataa tccgattaaa atgtcaaatg gtaaaattgc taccatgtta gtgaatgata aaaattctcc ctttgcatct aatgaaaagc tctcaaaatg cttaaatgaa cttgatgcaa agcttgctca aagagctcaa agaaacgaag caggaaatac ctcacaaaaa aaagtttcag ataaaaatga accgcttagt gaagatatag caactattta tgcactactt gatcatgcac gtcctttcgt agagggcaat ggaattgtac aacggttatt tgtgaataaa ctctctcaag ctgagaatca aaagcttgat ttttcagttg tgacacaaaa gcgtatggaa gatgcaattg ttagtgcagc aaatgacaat ttacagccta tgcaacatct gtttgaagat gcttctaatc cagaaaacat caaggttttg aaaacagcta taaatagaat gacacctgaa gaagcgcaag agattgcaag aggatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BaroA.17330.i.B3.GE36603
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN YTYAESGDPY TLINKLGIQD HQQLKIKCAH KVTKELVNVH QEPIPQKVDQ LYLKKLHKRL FGAIFDWAGE TRDNPIKMSN GKIATMLVND KNSPFASNEK LSKCLNELDA KLAQRAQRNE AGNTSQKKVS DKNEPLSEDI ATIYALLDHA RPFVEGNGIV QRLFVNKLSQ AENQKLDFSV VTQKRMEDAI VSAANDNLQP MQHLFEDASN PENIKVLKTA INRMTPEEAQ EIARGIITTP ISGKIYTGVY ESRQLDTIVI RTSNSLIXCN KSVIPREQFX KLKLGDKISF K
Validated NT Sequence
tttgnnnnnn ttaagaagga ganataccat ggctcaccac caccaccacc atatgaacta cacctatgct gaatctggag atccctatac gttaataaat aaacttggga tacaagatca ccagcaactt aagataaagt gtgcgcataa agtgacgaaa gaacttgtta atgtgcatca agaaccgata cctcaaaaag ttgatcagct ttacctaaaa aaacttcaca aacgcttatt tggagcgata tttgattggg ctggagaaac ccgtgataat ccgattaaaa tgtcaaatgg taaaattgct accatgttag tgaatgataa aaattctccc tttgcatcta atgaaaagct ctcaaaatgc ttaaatgaac ttgatgcaaa gcttgctcaa agagctcaaa gaaacgaagc aggaaatacc tcacaaaaaa aagtttcaga taaaaatgaa ccgcttagtg aagatatagc aactatttat gcactacttg atcatgcacg tcctttcgta gagggcaatg gaattgtaca acggttattt gtgaataaac tctctcaagc tgagaatcaa aagcttgatt tttcagttgt gacacaaaag cgtatggaag atgcaattgt tagtgcagca aatgacaatt tacagcctat gcaacatctg tttgaagatg cttctaatcc agaaaacatc aaggttttga aaacagctat aaatagaatg acacctgaag aagcgcaaga gattgcaaga ggaattatta cnacaccaat ctcaggaaaa atctatacag gtgtctatga aagtcgtcaa ttagatacta ttgtgataag aacatcgaat tctttgattg natgcaataa atctgttatt ccnagggagc agttccnaaa attaaagcta ggtgataaaa tcagctttaa ataagtgagt aananagnan nnnnnnnnnn nnncnccnnn nnnnnnnnnn nnnnnnnnnn aa
Expected Protein Sequence
MAHHHHHHMN YTYAESGDPY TLINKLGIQD HQQLKIKCAH KVTKELVNVH QEPIPQKVDQ LYLKKLHKRL FGAIFDWAGE TRDNPIKMSN GKIATMLVND KNSPFASNEK LSKCLNELDA KLAQRAQRNE AGNTSQKKVS DKNEPLSEDI ATIYALLDHA RPFVEGNGIV QRLFVNKLSQ AENQKLDFSV VTQKRMEDAI VSAANDNLQP MQHLFEDASN PENIKVLKTA INRMTPEEAQ EIARGIITTP ISGKIYTGVY ESRQLDTIVI RTSNSLIVCN KSVIPREQFQ KLKLGDKISF K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaact acacctatgc tgaatctgga gatccctata cgttaataaa taaacttggg atacaagatc accagcaact taagataaag tgtgcgcata aagtgacgaa agaacttgtt aatgtgcatc aagaaccgat acctcaaaaa gttgatcagc tttacctaaa aaaacttcac aaacgcttat ttggagcgat atttgattgg gctggagaaa cccgtgataa tccgattaaa atgtcaaatg gtaaaattgc taccatgtta gtgaatgata aaaattctcc ctttgcatct aatgaaaagc tctcaaaatg cttaaatgaa cttgatgcaa agcttgctca aagagctcaa agaaacgaag caggaaatac ctcacaaaaa aaagtttcag ataaaaatga accgcttagt gaagatatag caactattta tgcactactt gatcatgcac gtcctttcgt agagggcaat ggaattgtac aacggttatt tgtgaataaa ctctctcaag ctgagaatca aaagcttgat ttttcagttg tgacacaaaa gcgtatggaa gatgcaattg ttagtgcagc aaatgacaat ttacagccta tgcaacatct gtttgaagat gcttctaatc cagaaaacat caaggttttg aaaacagcta taaatagaat gacacctgaa gaagcgcaag agattgcaag aggaattatt acaacaccaa tctcaggaaa aatctataca ggtgtctatg aaagtcgtca attagatact attgtgataa gaacatcgaa ttctttgatt gtatgcaata aatctgttat tccaagggag cagttccaaa aattaaagct aggtgataaa atcagcttta aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BaroA.17330.i.B4.GE37274
HARVESTED ON: 8/28/2013
SEQUENCED ON: 9/3/2013
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML IPGKELRNLT DFKIMERTVC NSYVLHQRKE IERLAKVVFG NREKLTNHLE RIHDNPNSAK MLSETILHNP KSIAKLAGFG KYGINSPKRN KAEKSLSALG EAILRYGSDL KTSKEEVLRE HRAEQYRLAK EVSLPG
Validated NT Sequence
atcttactca cttagccagg taaacttacc tcttttgcaa gacggtattg ttctgctcta tgttctctca aaacttcttc tttagaagtt ttcaaatcac tcccatatct caaaattgct tcaccgagtg cagaaagact tttttcagct ttattacgtt ttggactgtt tattccatat tttccaaaac ctgcaagctt agcaatagat ttagggttgt gtaagatagt ttcagaaagc attttcgctg aatttggatt atcatgaatt cgttctagat gattggttaa tttttcacgg tttccaaata caacttttgc caaacgttca atttcttttc tttgatgtag aacataagaa ttacatacgg tcctttccat aattttaaag tctgttaaat tgcgtaattc ttttcctggg attaacatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHLI PGKELRNLTD FKIMERTVCN SYVLHQRKEI ERLAKVVFGN REKLTNHLER IHDNPNSAKM LSETILHNPK SIAKLAGFGK YGINSPKRNK AEKSLSALGE AILRYGSDLK TSKEEVLREH RAEQYRLAKE VSLPG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttaatcc caggaaaaga attacgcaat ttaacagact ttaaaattat ggaaaggacc gtatgtaatt cttatgttct acatcaaaga aaagaaattg aacgtttggc aaaagttgta tttggaaacc gtgaaaaatt aaccaatcat ctagaacgaa ttcatgataa tccaaattca gcgaaaatgc tttctgaaac tatcttacac aaccctaaat ctattgctaa gcttgcaggt tttggaaaat atggaataaa cagtccaaaa cgtaataaag ctgaaaaaag tctttctgca ctcggtgaag caattttgag atatgggagt gatttgaaaa cttctaaaga agaagttttg agagaacata gagcagaaca ataccgtctt gcaaaagagg taagtttacc tggctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat