BaroA.17330.h

Bep5 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaroA.17330.h
ORGANISM: Bartonella rochalimae ATCC BAA-1498
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaroA.17330.h.B2.GE36600 FIC 96 305
BaroA.17330.h.B3.GE36601 FIC and truncated OB-Fold 96 376
BaroA.17330.h.B4.GE37273 BID 387 524
BaroA.17330.h.B5.GE37444 N-term extended FIC 1 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: E6YKB8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MISIDNKSST ESIDISQKEL RKSVEMSINV YMINGLYILP EEFAFIKRYV TAADSFLRDD LSSSDFSLKD FLLKDYLLFR DLTRKKFNIP VDEVLLNGYI YANSLTLKNK YELQGIEFQD ICAYDVLEAE YRLKEEPLPE KFDSSYLKYL HKCLFEKTFE WAGHTCDIPF TFLDGTVTTV PVNNKIKDGL ERIDQILAEK NNLKGLSHKE FVYEASMIFA LFSNLRPFAV GNGCAQRIFM EQIAEAAGYR LDFSVVTEQR MRSANNAALP RDGRAVGNIT PMCHLFEDIS NPEKVDVLRE FMNAIRNAGS FDEYAKVINR VIVVPNEGST YTGICKGYSP DSIMLMINDK YVICKKDYFA PETLTALRLG DKLTFRASVP ENFENILIPE EKVVPLTEKE IVARIAGNIF VQKSRQKIKT LSKSIYKSSK ILDADINLVN IDSGFGEQYI RQNMSLFKYM SKLAGTEILG IDSPRRKQAK KDYFNLTQEF DNYISIVVSL REHILQEHQE KQERCQQAIK MPSQAVQNIL SMSEEDQIKA LNANPSLCRE ITGLVDKVKD RLTFWEYQTL DDRDYEKLAK SVGISFSRAQ KVTEIIKKAQ KVCQLTRSRA HESKKMEHNL LQI
NT Sequence
gtgattagta tagacaataa atcgagtaca gagtcgatag atatttctca aaaggaacta cgaaagtctg tagagatgtc tatcaatgta tatatgatta atgggcttta cattcttcct gaggagtttg cctttataaa gaggtatgtg actgcagctg atagtttttt acgcgatgat ctctcatcta gtgatttttc acttaaagat tttttactta aagattattt gttattcaga gatcttacac ggaagaaatt taatattccg gtggatgagg ttttgctaaa tggctatatc tatgctaata gtttaacgct gaaaaataaa tatgaattgc agggcataga gtttcaagat atatgtgcct atgatgtatt agaagcagag tacaggctta aagaagaacc gctaccagaa aagtttgatt catcttatct aaaatatctt cataaatgtt tgtttgagaa aacgttcgaa tgggctgggc atacctgtga tattccattt acatttttag atggcacagt taccacggtg ccagtcaata ataaaattaa agacggttta gagagaatcg atcaaatact agccgaaaag aataatttga aaggtttatc acataaagag tttgtttatg aagcatcgat gatatttgct ttgtttagta atttaagacc ttttgcagtt ggaaatggat gtgcacaacg gatatttatg gaacaaatag ccgaagcagc agggtatagg cttgacttct cggttgtaac agaacaacgc atgcgatctg ctaataatgc tgcgttacca cgtgatggca gagctgtagg caatatcact cctatgtgtc atctatttga agatatctcc aatccagaaa aagtggatgt tttaagagaa ttcatgaatg ccataaggaa tgcgggatct tttgatgagt atgcaaaggt gataaatagg gttattgtag tgccaaatga aggttctacc tatacgggta tttgtaaagg ttatagccca gattccatca tgctcatgat aaatgataag tatgttatat gcaaaaaaga ttattttgca ccagaaacat taacagcatt acggcttggt gacaaactta cttttagagc ttctgtgcca gaaaactttg agaatattct tattccagaa gaaaaagtag ttcctttgac ggaaaaagaa attgttgcaa gaattgcagg taatattttc gttcaaaaga gtcgacaaaa aatcaaaact ttatcaaaat ctatctataa aagctcgaaa atattggatg ctgatatcaa tcttgttaat atagattcag gtttcggtga acagtatatc agacagaata tgagtctttt taaatatatg tctaaattgg caggtacgga aatattgggt attgacagtc cacggcgcaa acaagctaag aaagattact ttaatctcac tcaggaattt gataattata taagtattgt agtctctctt agagagcaca ttcttcaaga gcaccaagaa aaacaagaac gttgtcagca agcgataaaa atgcctagtc aagcagtgca aaatatcctt tctatgtctg aagaagatca gatcaaagct ttaaatgcca atccctcact ttgcagagaa attacaggtc ttgtggataa agtcaaagat cgtcttacat tttgggagta tcaaaccctt gatgatagag attatgagaa gctcgctaaa agtgtcggta tatcattcag tagagcacag aaagtcacag aaattattaa gaaagctcaa aaagtatgtc agctaactag aagcagagct catgaatcga aaaaaatgga gcataatcta ttacaaatat aa
Details for BaroA.17330.h.B2.GE36600
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML NGYIYANSLT LKNKYELQGI EFQDICAYDV LEAEYRLKEE PLPEKFDSSY LKYLHKCLFE KTFEWAGHTC DIPFTFLDGT VTTVPVNNKI KDGLERIDQI LAEKNNLKGL SHKEFVYEAS MIFALFSNLR PFAVGNGCAQ RIFMEQIAEA AGYRLDFSVV TEQRMRSANN AALPRDGRAV GNITPMCHLF EDISNPEKVD VLREFMNAI
Validated NT Sequence
ttttntttac tttaagaagg aganatacca tggctcacca ccaccaccac catatgctaa atggctatat ctatgctaat agtttaacgc tgaaaaataa atatgaattg cagggcatag agtttcaaga tatatgtgcc tatgatgtat tagaagcaga gtacaggctt aaagaagaac cgctaccaga aaagtttgat tcatcttatc taaaatatct tcataaatgt ttgtttgaga aaacgttcga atgggctggg catacctgtg atattccatt tacattttta gatggcacag ttaccacggt gccagtcaat aataaaatta aagacggttt agagagaatc gatcaaatac tagccgaaaa gaataatttg aaaggtttat cacataaaga gtttgtttat gaagcatcga tgatatttgc tttgtttagt aatttaagac cttttgcagt tggaaatgga tgtgcacaac ggatatttat ggaacaaata gccgaagcag cagggtatag gcttgacttc tcggttgtaa cagaacaacg catgcgatct gctaataatg ctgcgttacc acgtgatggc agagctgtag gcaatatcac tcctatgtgt catctatttg aagatatctc caatccagaa aaagtggatg ttttaagaga attcatgaat gccatataag tgagtaagat aggatccggc tgnnnnnnnc ccccnnnnnn nngnnnaaaa annnnnnnna nnnnnnctat gnnncatnnn ncnngnntnn nnatgnnnnn nngnnnnnnn nnncncnnnn ttgnannnnn cannannnnt nncggctgnt nnnnnnngcn nnnntcngtg agnatanngn nnnnnnnnnn nnnnnnannn nnnnnnnnnn nnntnnnnnn nnnnnntnnn nnnncnnnnn cnnnna
Expected Protein Sequence
MAHHHHHHML NGYIYANSLT LKNKYELQGI EFQDICAYDV LEAEYRLKEE PLPEKFDSSY LKYLHKCLFE KTFEWAGHTC DIPFTFLDGT VTTVPVNNKI KDGLERIDQI LAEKNNLKGL SHKEFVYEAS MIFALFSNLR PFAVGNGCAQ RIFMEQIAEA AGYRLDFSVV TEQRMRSANN AALPRDGRAV GNITPMCHLF EDISNPEKVD VLREFMNAI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctaa atggctatat ctatgctaat agtttaacgc tgaaaaataa atatgaattg cagggcatag agtttcaaga tatatgtgcc tatgatgtat tagaagcaga gtacaggctt aaagaagaac cgctaccaga aaagtttgat tcatcttatc taaaatatct tcataaatgt ttgtttgaga aaacgttcga atgggctggg catacctgtg atattccatt tacattttta gatggcacag ttaccacggt gccagtcaat aataaaatta aagacggttt agagagaatc gatcaaatac tagccgaaaa gaataatttg aaaggtttat cacataaaga gtttgtttat gaagcatcga tgatatttgc tttgtttagt aatttaagac cttttgcagt tggaaatgga tgtgcacaac ggatatttat ggaacaaata gccgaagcag cagggtatag gcttgacttc tcggttgtaa cagaacaacg catgcgatct gctaataatg ctgcgttacc acgtgatggc agagctgtag gcaatatcac tcctatgtgt catctatttg aagatatctc caatccagaa aaagtggatg ttttaagaga attcatgaat gccatatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BaroA.17330.h.B3.GE36601
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML NGYIYANSLT LKNKYELQGI EFQDICAYDV LEAEYRLKEE PLPEKFDSSY LKYLHKCLFE KTFEWAGHTC DIPFTFLDGT VTTVPVNNKI KDGLERIDQI LAEKNNLKGL SHKEFVYEAS MIFALFSNLR PFAVGNGCAQ RIFMEQIAEA AGYRLDFSVV TEQRMRSANN AALPRDGRAV GNITPMCHLF EDISNPEKVD VLREFMNAIR NAGSFDEYAK VINRVIVVPN EGSTYTGICK GYSPDSIMLM INDKYVICKK DXFAPETLTA LRLGDKLTFR
Validated NT Sequence
attttgntta nctttaagaa ggaganatac catggctcac caccaccacc accatatgct aaatggctat atctatgcta atagtttaac gctgaaaaat aaatatgaat tgcagggcat agagtttcaa gatatatgtg cctatgatgt attagaagca gagtacaggc ttaaagaaga accgctacca gaaaagtttg attcatctta tctaaaatat cttcataaat gtttgtttga gaaaacgttc gaatgggctg ggcatacctg tgatattcca tttacatttt tagatggcac agttaccacg gtgccagtca ataataaaat taaagacggt ttagagagaa tcgatcaaat actagccgaa aagaataatt tgaaaggttt atcacataaa gagtttgttt atgaagcatc gatgatattt gctttgttta gtaatttaag accttttgca gttggaaatg gatgtgcaca acggatattt atggaacaaa tagccgaagc agcagggtat aggcttgact tctcggttgt aacagaacaa cgcatgcgat ctgctaataa tgctgcgtta ccacgtgatg gcagagctgt aggcaatatc actcctatgt gtcatctatt tgaagatatc tccaatccag aaaaagtgga tgttttaaga gaattcatga atgccataag gaatgcggga tcttttgatg agtatgcaaa ggtgataaat agggttattg tagtgccaaa tgaaggttct acctatacgg gtatttgtaa aggttatagc ccagattcca tcatgctcat gataaatgat aagtatgtta tatgcaaaaa agattanttt gcaccagaaa cattaacagc attacggctt ggtgacaaac ttacttttag ataagtgagt aagannnnnn nnnnnnnnnn nnccnnnnnn nnnnnnnnnn nnncnnnngg ggnnnnnnnn nccntt
Expected Protein Sequence
MAHHHHHHML NGYIYANSLT LKNKYELQGI EFQDICAYDV LEAEYRLKEE PLPEKFDSSY LKYLHKCLFE KTFEWAGHTC DIPFTFLDGT VTTVPVNNKI KDGLERIDQI LAEKNNLKGL SHKEFVYEAS MIFALFSNLR PFAVGNGCAQ RIFMEQIAEA AGYRLDFSVV TEQRMRSANN AALPRDGRAV GNITPMCHLF EDISNPEKVD VLREFMNAIR NAGSFDEYAK VINRVIVVPN EGSTYTGICK GYSPDSIMLM INDKYVICKK DYFAPETLTA LRLGDKLTFR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctaa atggctatat ctatgctaat agtttaacgc tgaaaaataa atatgaattg cagggcatag agtttcaaga tatatgtgcc tatgatgtat tagaagcaga gtacaggctt aaagaagaac cgctaccaga aaagtttgat tcatcttatc taaaatatct tcataaatgt ttgtttgaga aaacgttcga atgggctggg catacctgtg atattccatt tacattttta gatggcacag ttaccacggt gccagtcaat aataaaatta aagacggttt agagagaatc gatcaaatac tagccgaaaa gaataatttg aaaggtttat cacataaaga gtttgtttat gaagcatcga tgatatttgc tttgtttagt aatttaagac cttttgcagt tggaaatgga tgtgcacaac ggatatttat ggaacaaata gccgaagcag cagggtatag gcttgacttc tcggttgtaa cagaacaacg catgcgatct gctaataatg ctgcgttacc acgtgatggc agagctgtag gcaatatcac tcctatgtgt catctatttg aagatatctc caatccagaa aaagtggatg ttttaagaga attcatgaat gccataagga atgcgggatc ttttgatgag tatgcaaagg tgataaatag ggttattgta gtgccaaatg aaggttctac ctatacgggt atttgtaaag gttatagccc agattccatc atgctcatga taaatgataa gtatgttata tgcaaaaaag attattttgc accagaaaca ttaacagcat tacggcttgg tgacaaactt acttttagat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaroA.17330.h.B4.GE37273
HARVESTED ON: 8/28/2013
SEQUENCED ON: 9/3/2013
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML IPEEKVVPLT EKEIVARIAG NIFVQKSRQK IKTLSKSIYK SSKILDADIN LVNIDSGFGE QYIRQNMSLF KYMSKLAGTE ILGIDSPRRK QAKKDYFNLT QEFDNYISIV VSLREHILQE HQEKQERCQQ AIKMPSQ
Validated NT Sequence
atcttactca cttattgact aggcattttt atcgcttgct gacaacgttc ttgtttttct tggtgctctt gaagaatgtg ctctctaaga gagactacaa tacttatata attatcaaat tcctgagtga gattaaagta atctttctta gcttgtttgc gccgtggact gtcaataccc aatatttccg tacctgccaa tttagacata tatttaaaaa gactcatatt ctgtctgata tactgttcac cgaaacctga atctatatta acaagattga tatcagcatc caatattttc gagcttttat agatagattt tgataaagtt ttgatttttt gtcgactctt ttgaacgaaa atattacctg caattcttgc aacaatttct ttttccgtca aaggaactac tttttcttct ggaataagca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHLI PEEKVVPLTE KEIVARIAGN IFVQKSRQKI KTLSKSIYKS SKILDADINL VNIDSGFGEQ YIRQNMSLFK YMSKLAGTEI LGIDSPRRKQ AKKDYFNLTQ EFDNYISIVV SLREHILQEH QEKQERCQQA IKMPSQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcttattc cagaagaaaa agtagttcct ttgacggaaa aagaaattgt tgcaagaatt gcaggtaata ttttcgttca aaagagtcga caaaaaatca aaactttatc aaaatctatc tataaaagct cgaaaatatt ggatgctgat atcaatcttg ttaatataga ttcaggtttc ggtgaacagt atatcagaca gaatatgagt ctttttaaat atatgtctaa attggcaggt acggaaatat tgggtattga cagtccacgg cgcaaacaag ctaagaaaga ttactttaat ctcactcagg aatttgataa ttatataagt attgtagtct ctcttagaga gcacattctt caagagcacc aagaaaaaca agaacgttgt cagcaagcga taaaaatgcc tagtcaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BaroA.17330.h.B5.GE37444
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 36kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMI SIDNKSSTES IDISQKELRK SVEMSINVYM INGLYILPEE FAFIKRYVTA ADSFLRDDLS SSDFSLKDFL LKDYLLFRDL TRKKFNIPVD EVLLNGYIYA NSLTLKNKYE LQGIEFQDIC AYDVLEAEYR LKEEPLPEKF DSSYLKYLHK CLFEKTFEWA GHTCDIPFTF LDGTVTTVPV NNKIKDGLER IDQILAEKNN LKGLSHKEFV YEASMIFALF SNLRPFAVGN GCAQRIFMEQ IAEAAGYRLD FSVVTEQRMR SANNAALPRD GRAVGNITPM CHLFEDISNP EKVDVLREFM NAI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgatta gtatagacaa taaatcgagt acagagtcga tagatatttc tcaaaaggaa ctacgaaagt ctgtagagat gtctatcaat gtatatatga ttaatgggct ttacattctt cctgaggagt ttgcctttat aaagaggtat gtgactgcag ctgatagttt tttacgcgat gatctctcat ctagtgattt ttcacttaaa gattttttac ttaaagatta tttgttattc agagatctta cacggaagaa atttaatatt ccggtggatg aggttttgct aaatggctat atctatgcta atagtttaac gctgaaaaat aaatatgaat tgcagggcat agagtttcaa gatatatgtg cctatgatgt attagaagca gagtacaggc ttaaagaaga accgctacca gaaaagtttg attcatctta tctaaaatat cttcataaat gtttgtttga gaaaacgttc gaatgggctg ggcatacctg tgatattcca tttacatttt tagatggcac agttaccacg gtgccagtca ataataaaat taaagacggt ttagagagaa tcgatcaaat actagccgaa aagaataatt tgaaaggttt atcacataaa gagtttgttt atgaagcatc gatgatattt gctttgttta gtaatttaag accttttgca gttggaaatg gatgtgcaca acggatattt atggaacaaa tagccgaagc agcagggtat aggcttgact tctcggttgt aacagaacaa cgcatgcgat ctgctaataa tgctgcgtta ccacgtgatg gcagagctgt aggcaatatc actcctatgt gtcatctatt tgaagatatc tccaatccag aaaaagtgga tgttttaaga gaattcatga atgccatatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat