| CENTER ID: | BaroA.17330.f |
| ORGANISM: | Bartonella rochalimae ATCC BAA-1498 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | purified |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaroA.17330.f.B2.GE36596 | FIC | 91 | 316 | ||
| BaroA.17330.f.B3.GE36597 | FIC and truncated OB-Fold | 91 | 383 | ||
| BaroA.17330.f.B5.GE37443 | N-term extended FIC | 1 | 316 |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BaroA.17330.f.B5.PW37445 | N-term extended FIC | 1 | 316 |
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|685782.3.peg.1338 |
| RefSeq: | WP_051668877.1 |
| UniProt: | E6YLF6 |
| HARVESTED ON: | 2/8/2013 |
| SEQUENCED ON: | 2/14/2013 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 2/14/2013 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
| HARVESTED ON: | 10/31/2013 |
| SEQUENCED ON: | 11/5/2013 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 14 |
| PURIFICATION DATe: | 2/18/2014 |
| CONCENTRATION: | 5.91mg/ml |
| OBSERVED MW: | 36kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 14 |