BaroA.17330.e

Bep8 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaroA.17330.e
ORGANISM: Bartonella rochalimae ATCC BAA-1498
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaroA.17330.e.B1.GE36081 full length 1 543
BaroA.17330.e.B2.GE36594 FIC 11 239
BaroA.17330.e.B3.GE36595 FIC and truncated OB-Fold 11 296
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaroA.17330.e.B1.PS01837 full length 1 543 unavailable
BaroA.17330.e.B1.PS01838 full length 1 543 unavailable
BaroA.17330.e.B2.PW37281 FIC 11 239

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|685782.3.peg.1334
RefSeq: WP_035007368.1
UniProt: E6YLF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVFSDISLAR NYVYPHMKVL KNKHNITSTK KLAYECKRFA KKVMINLNNE PLPQEFDSSY LKYIHQRLFE STFEWAGYTR DLSFTFDDGT VAEMPMMKVP DSDIFYVQGK NIQESLKKFD QLLASKNNLQ GLSREEFIDE AAKLFAFLNS IAPFRAGNEP TQRIFFEKLA KAAGHQLDFS VATEKRIMRA CIDSMTLEGD GAHKEMKLLF EDISCPQKII VLKDFIRRIP RLERERLNDE YVIMPKEGIT YTGIYKIGTV NSVIIETADS HVLCVKDYFP PEQLKTLKPG DVCTVTVPID IDQILIPAEK MAPLKEEEII RKVSRTNCIK ESQKEIDYLL KFVYRDITVL DEQIMCIRKC PETSKQLSEQ IRKSPQAFGK LAGFKVFGIR SPKRRIAERN IEKLTSEIEK HGEIVKNVKD EISLEFEKDQ KRVGQVVKKA SKKIQDILNL PKDEQKNALE ASPLLYEELR SFLNEVNSRL SAREKDYICD YHDKKLAESI GVSEMHAMTI IKTVNKSAKL CKQFEKIKEI KKVDCSEIMA MAV
NT Sequence
atggtatttt ctgatatatc tttagctcgt aattatgttt atcctcacat gaaagtatta aagaataaac ataatataac gagtactaaa aaacttgcat acgaatgtaa gcgttttgca aaaaaagtaa tgatcaattt aaataacgaa ccattgcccc aagaatttga ttcctcttac ttaaaatata tccatcaacg cttattcgag agcacatttg aatgggctgg atatactcgt gatctttcgt ttacgtttga tgatggcact gttgctgaaa tgccaatgat gaaagttcca gactcagata ttttttatgt acagggtaag aacattcaag aaagcttaaa gaaatttgat caactacttg cttcaaagaa taatttgcaa ggtttatctc gtgaagagtt tattgatgaa gctgcgaaac tttttgcttt tctcaactct atagctcctt tcagagctgg taatgagcct acacaacgaa tattttttga aaaacttgct aaggctgcag gtcatcaact agatttttca gttgcgacag agaaacgtat aatgcgtgcc tgtattgatt caatgactct agagggtgat ggggctcata aagagatgaa acttctgttt gaagatatct cttgtcccca aaaaataatt gttttaaagg attttattcg acgcatacct agattggagc gcgagcgttt gaatgatgaa tatgttataa tgccaaagga aggtattact tatacaggta tttataaaat aggtactgta aattccgtta taatcgaaac agcggattct catgttctat gcgttaaaga ttattttcca ccggaacaac ttaagacatt aaaacctggt gatgtgtgta ccgttacagt tccaatagat atagatcaaa ttctcattcc agcagaaaaa atggctcctt taaaggaaga agaaataatc agaaaagttt cacgtactaa ttgtattaaa gaaagccaaa aggaaattga ttatctttta aagtttgttt atagagacat aacagtatta gacgaacaga taatgtgtat tcgtaaatgt cctgaaacaa gtaaacagct ttccgaacag attagaaaat ctcctcaagc ttttggtaag ctagctggtt ttaaagtatt tggtataagg agtccaaaac gtaggatagc tgaaagaaat attgaaaaac tcacttcgga gattgaaaaa catggggaaa ttgtgaaaaa tgttaaagat gaaatttctc tagaatttga aaaagatcaa aagcgtgttg ggcaagtggt gaaaaaagct agtaaaaaaa tacaagatat tcttaatttg ccaaaggatg agcaaaaaaa tgccttagag gcctctcctc ttctttacga agagcttcgc agctttctga atgaagtcaa ttctcgtctt tcagcaagag agaaagatta catctgtgac tatcatgata aaaaacttgc cgaaagcatt ggtgtatcag aaatgcacgc aatgacaatt ataaaaactg ttaacaaaag cgcaaaattg tgtaaacaat ttgaaaagat caaagaaatt aaaaaagttg attgttcaga aataatggct atggccgttt ga
Details for BaroA.17330.e.B1.GE36081
HARVESTED ON: 10/2/2012
SEQUENCED ON: 2/22/2016
EXPECTED MW: 63kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 67
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV FSDISLARNY VYPHMKVLKN KHNITSTKKL AYECKRFAKK VMINLNNEPL PQEFDSSYLK YIHQRLFEST FEWAGYTRDL SFTFDDGTVA EMPMMKVPDS DIFYVQGKNI QESLKKFDQL LASKNNLQGL SREEFIDEAA KLFAFLNSIA PFRAGNEPTQ RIFFEKLAKA AGHQLDFSVA TEKRIMRACI DSMTLEGDGA HKEMKLLFED ISCPQKIIVL KDFIRRIPRL ERERLNDEYV IMPKEGITYT GIYKIGTVNS VIIETADSHV LCVKDYFPPE QLKTLKPGDV CTVTVXIDID QILIPAEKMA PLKEEEIIRK VSRTNCIKES QKXXXFXXXX XRETTTVXXX XXXXXXXXXX XXMXHXHHHH MTPLKTVEPF VIRVDNSTGI IETVSALKET PNNYDEAITR YFASKYVRAR EGFQLSEAEY NFRLISLLSS PEEQNRFAKW YSGNNPESPQ NIYHNMTAKV TIKSISFLSK DLIQVRYYKT IREXNGKENI SXXVSIXNFS YINAHISTED RLINPLGFQV SE
Validated NT Sequence
atggctcacc accaccacca ccatatggta ttttctgata tatctttagc tcgtaattat gtttatcctc acatgaaagt attaaagaat aaacataata taacgagtac taaaaaactt gcatacgaat gtaagcgttt tgcaaaaaaa gtaatgatca atttaaataa cgaaccattg ccccaagaat ttgattcctc ttacttaaaa tatatccatc aacgcttatt cgagagcaca tttgaatggg ctggatatac tcgtgatctt tcgtttacgt ttgatgatgg cactgttgct gaaatgccaa tgatgaaagt tccagactca gatatttttt atgtacaggg taagaacatt caagaaagct taaagaaatt tgatcaacta cttgcttcaa agaataattt gcaaggttta tctcgtgaag agtttattga tgaagctgcg aaactttttg cttttctcaa ctctatagct cctttcagag ctggtaatga gcctacacaa cgaatatttt ttgaaaaact tgctaaggct gcaggtcatc aactagattt ttcagttgcg acagagaaac gtataatgcg tgcctgtatt gattcaatga ctctagaggg tgatggggct cataaagaga tgaaacttct gtttgaagat atctcttgtc cccaaaaaat aattgtttta aaggatttta ttcgacgcat acctagattg gagcgcgagc gtttgaatga tgaatatgtt ataatgccaa aggaaggtat tacttataca ggtatttata aaataggtac tgtaaattcc gttataatcg aaacagcgga ttctcatgtt ctatgcgtta aagattattt tccaccggaa caacttaaga cattaaaacc tggtgatgtg tgtaccgtta cagttncaat agatatagat caaattctca ttccagcaga aaaaatggct cctttaaagg aagaagaaat aatcagaaaa gtttcacgta ctaattgtat taaagaaagc caaaagnnnn nnnattttnn tnnnnnntnn natagggaga ccacaacggt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatgn ctcaccncca ccaccaccat atgacaccnt taaaaacagt agagccnttt gttatccgcg ttgataattc aacaggtatt attgagacag taagtgcctt aaaagaaacg ccaaacaatt atgatgaagc aataacacgt tattttgcta gcaaatatgt tcgtgcgcgc gaaggttttc aattatcaga ggcggaatac aattttcgct tgatttctct tttatcttca ccagaagaac aaaaccgttt tgcaaaatgg tattcgggca acaatccaga aagtccgcaa aatatctatc acaacatgac tgctaaggtc acaatcaagt caatctcctt tttaagcaaa gatcttattc aagtgcgtta ctataaaacg atcagagaan ngaatggaaa agaaaatatt tcccannngg tctcgatcnt caatttttcc tacattaatg cacacatttc aacagaagac cgactaatca acccgcttgg ttttcaagtg tcagaata
Expected Protein Sequence
MAHHHHHHMV FSDISLARNY VYPHMKVLKN KHNITSTKKL AYECKRFAKK VMINLNNEPL PQEFDSSYLK YIHQRLFEST FEWAGYTRDL SFTFDDGTVA EMPMMKVPDS DIFYVQGKNI QESLKKFDQL LASKNNLQGL SREEFIDEAA KLFAFLNSIA PFRAGNEPTQ RIFFEKLAKA AGHQLDFSVA TEKRIMRACI DSMTLEGDGA HKEMKLLFED ISCPQKIIVL KDFIRRIPRL ERERLNDEYV IMPKEGITYT GIYKIGTVNS VIIETADSHV LCVKDYFPPE QLKTLKPGDV CTVTVPIDID QILIPAEKMA PLKEEEIIRK VSRTNCIKES QKEIDYLLKF VYRDITVLDE QIMCIRKCPE TSKQLSEQIR KSPQAFGKLA GFKVFGIRSP KRRIAERNIE KLTSEIEKHG EIVKNVKDEI SLEFEKDQKR VGQVVKKASK KIQDILNLPK DEQKNALEAS PLLYEELRSF LNEVNSRLSA REKDYICDYH DKKLAESIGV SEMHAMTIIK TVNKSAKLCK QFEKIKEIKK VDCSEIMAMA V
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtat tttctgatat atctttagct cgtaattatg tttatcctca catgaaagta ttaaagaata aacataatat aacgagtact aaaaaacttg catacgaatg taagcgtttt gcaaaaaaag taatgatcaa tttaaataac gaaccattgc cccaagaatt tgattcctct tacttaaaat atatccatca acgcttattc gagagcacat ttgaatgggc tggatatact cgtgatcttt cgtttacgtt tgatgatggc actgttgctg aaatgccaat gatgaaagtt ccagactcag atatttttta tgtacagggt aagaacattc aagaaagctt aaagaaattt gatcaactac ttgcttcaaa gaataatttg caaggtttat ctcgtgaaga gtttattgat gaagctgcga aactttttgc ttttctcaac tctatagctc ctttcagagc tggtaatgag cctacacaac gaatattttt tgaaaaactt gctaaggctg caggtcatca actagatttt tcagttgcga cagagaaacg tataatgcgt gcctgtattg attcaatgac tctagagggt gatggggctc ataaagagat gaaacttctg tttgaagata tctcttgtcc ccaaaaaata attgttttaa aggattttat tcgacgcata cctagattgg agcgcgagcg tttgaatgat gaatatgtta taatgccaaa ggaaggtatt acttatacag gtatttataa aataggtact gtaaattccg ttataatcga aacagcggat tctcatgttc tatgcgttaa agattatttt ccaccggaac aacttaagac attaaaacct ggtgatgtgt gtaccgttac agttccaata gatatagatc aaattctcat tccagcagaa aaaatggctc ctttaaagga agaagaaata atcagaaaag tttcacgtac taattgtatt aaagaaagcc aaaaggaaat tgattatctt ttaaagtttg tttatagaga cataacagta ttagacgaac agataatgtg tattcgtaaa tgtcctgaaa caagtaaaca gctttccgaa cagattagaa aatctcctca agcttttggt aagctagctg gttttaaagt atttggtata aggagtccaa aacgtaggat agctgaaaga aatattgaaa aactcacttc ggagattgaa aaacatgggg aaattgtgaa aaatgttaaa gatgaaattt ctctagaatt tgaaaaagat caaaagcgtg ttgggcaagt ggtgaaaaaa gctagtaaaa aaatacaaga tattcttaat ttgccaaagg atgagcaaaa aaatgcctta gaggcctctc ctcttcttta cgaagagctt cgcagctttc tgaatgaagt caattctcgt ctttcagcaa gagagaaaga ttacatctgt gactatcatg ataaaaaact tgccgaaagc attggtgtat cagaaatgca cgcaatgaca attataaaaa ctgttaacaa aagcgcaaaa ttgtgtaaac aatttgaaaa gatcaaagaa attaaaaaag ttgattgttc agaaataatg gctatggccg tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BaroA.17330.e.B2.GE36594
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 28kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN YVYPHMKVLK NKHNITSTKK LAYECKRFAK KVMINLNNEP LPQEFDSSYL KYIHQRLFES TFEWAGYTRD LSFTFDDGTV AEMPMMKVPD SDIFYVQGKN IQESLKKFDQ LLASKNNLQG LSREEFIDEA AKLFAFLNSI APFRAGNEPT QRIFFEKLAK AAGHQLDFSV ATEKRIMRAC IDSMTLEGDG AHKEMKLLFE DISCPQKIIV LKDFIRRIPR LERERLND
Validated NT Sequence
atcttactca cttaatcatt caaacgctcg cgctccaatc taggtatgcg tcgaataaaa tcctttaaaa caattatttt ttggggacaa gagatatctt caaacagaag tttcatctct ttatgagccc catcaccctc tagagtcatt gaatcaatac aggcacgcat tatacgtttc tctgtcgcaa ctgaaaaatc tagttgatga cctgcagcct tagcaagttt ttcaaaaaat attcgttgtg taggctcatt accagctctg aaaggagcta tagagttgag aaaagcaaaa agtttcgcag cttcatcaat aaactcttca cgagataaac cttgcaaatt attctttgaa gcaagtagtt gatcaaattt ctttaagctt tcttgaatgt tcttaccctg tacataaaaa atatctgagt ctggaacttt catcattggc atttcagcaa cagtgccatc atcaaacgta aacgaaagat cacgagtata tccagcccat tcaaatgtgc tctcgaataa gcgttgatgg atatatttta agtaagagga atcaaattct tggggcaatg gttcgttatt taaattgatc attacttttt ttgcaaaacg cttacattcg tatgcaagtt ttttagtact cgttatatta tgtttattct ttaatacttt catgtgagga taaacataat tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMN YVYPHMKVLK NKHNITSTKK LAYECKRFAK KVMINLNNEP LPQEFDSSYL KYIHQRLFES TFEWAGYTRD LSFTFDDGTV AEMPMMKVPD SDIFYVQGKN IQESLKKFDQ LLASKNNLQG LSREEFIDEA AKLFAFLNSI APFRAGNEPT QRIFFEKLAK AAGHQLDFSV ATEKRIMRAC IDSMTLEGDG AHKEMKLLFE DISCPQKIIV LKDFIRRIPR LERERLND
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt atgtttatcc tcacatgaaa gtattaaaga ataaacataa tataacgagt actaaaaaac ttgcatacga atgtaagcgt tttgcaaaaa aagtaatgat caatttaaat aacgaaccat tgccccaaga atttgattcc tcttacttaa aatatatcca tcaacgctta ttcgagagca catttgaatg ggctggatat actcgtgatc tttcgtttac gtttgatgat ggcactgttg ctgaaatgcc aatgatgaaa gttccagact cagatatttt ttatgtacag ggtaagaaca ttcaagaaag cttaaagaaa tttgatcaac tacttgcttc aaagaataat ttgcaaggtt tatctcgtga agagtttatt gatgaagctg cgaaactttt tgcttttctc aactctatag ctcctttcag agctggtaat gagcctacac aacgaatatt ttttgaaaaa cttgctaagg ctgcaggtca tcaactagat ttttcagttg cgacagagaa acgtataatg cgtgcctgta ttgattcaat gactctagag ggtgatgggg ctcataaaga gatgaaactt ctgtttgaag atatctcttg tccccaaaaa ataattgttt taaaggattt tattcgacgc atacctagat tggagcgcga gcgtttgaat gattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BaroA.17330.e.B3.GE36595
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN YVYPHMKVLK NKHNITSTKK LAYECKRFAK KVMINLNNEP LPQEFDSSYL KYIHQRLFES TFEWAGYTRD LSFTFDDGTV AEMPMMKVPD SDIFYVQGKN IQESLKKFDQ LLASKNNLQG LSREEFIDEA AKLFAFLNSI APFRAGNEPT QRIFFEKLAK AAGHQLDFSV ATEKRIMRAC IDSMTLEGDG AHKEMKLLFE DISCPQKIIV LKDFIRRIPR LERERLNDEY VIMPKEGITY TGIYKIGTVN SVIIETAXSH VLCVKDYFPP EQLKTLKPGX VCTVT
Validated NT Sequence
tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt atgtttatcc tcacatgaaa gtattaaaga ataaacataa tataacgagt actaaaaaac ttgcatacga atgtaagcgt tttgcaaaaa aagtaatgat caatttaaat aacgaaccat tgccccaaga atttgattcc tcttacttaa aatatatcca tcaacgctta ttcgagagca catttgaatg ggctggatat actcgtgatc tttcgtttac gtttgatgat ggcactgttg ctgaaatgcc aatgatgaaa gttccagact cagatatttt ttatgtacag ggtaagaaca ttcaagaaag cttaaagaaa tttgatcaac tacttgcttc aaagaataat ttgcaaggtt tatctcgtga agagtttatt gatgaagctg cgaaactttt tgcttttctc aactctatag ctcctttcag agctggtaat gagcctacac aacgaatatt ttttgaaaaa cttgctaagg ctgcaggtca tcaactagat ttttcagttg cgacagagaa acgtataatg cgtgcctgta ttgattcaat gactctagag ggtgatgggg ctcataaaga gatgaaactt ctgtttgaag atatctcttg tccccaaaaa ataattgttt taaaggattt tattcgacgc atacctagat tggagcgcga gcgtttgaat gatgaatatg ttataatgcc aaaggaaggt attacttata caggtattta taaaataggt actgtaaatt ccgttataat cgaaacagcg gantctcatg ttctatgcgt taaagattat tttccaccgg aacaacttaa gacattaaaa cctggtgang tgtgtaccgt tacntaantg agtaanatag nannnnnnnn nnnnnnnccn nnnnnnnnan nnnnnnnnnn naaaaaaann nnnnnntnnn ntnnnnnnnn cnnnnnnnnn nnnc
Expected Protein Sequence
MAHHHHHHMN YVYPHMKVLK NKHNITSTKK LAYECKRFAK KVMINLNNEP LPQEFDSSYL KYIHQRLFES TFEWAGYTRD LSFTFDDGTV AEMPMMKVPD SDIFYVQGKN IQESLKKFDQ LLASKNNLQG LSREEFIDEA AKLFAFLNSI APFRAGNEPT QRIFFEKLAK AAGHQLDFSV ATEKRIMRAC IDSMTLEGDG AHKEMKLLFE DISCPQKIIV LKDFIRRIPR LERERLNDEY VIMPKEGITY TGIYKIGTVN SVIIETADSH VLCVKDYFPP EQLKTLKPGD VCTVT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt atgtttatcc tcacatgaaa gtattaaaga ataaacataa tataacgagt actaaaaaac ttgcatacga atgtaagcgt tttgcaaaaa aagtaatgat caatttaaat aacgaaccat tgccccaaga atttgattcc tcttacttaa aatatatcca tcaacgctta ttcgagagca catttgaatg ggctggatat actcgtgatc tttcgtttac gtttgatgat ggcactgttg ctgaaatgcc aatgatgaaa gttccagact cagatatttt ttatgtacag ggtaagaaca ttcaagaaag cttaaagaaa tttgatcaac tacttgcttc aaagaataat ttgcaaggtt tatctcgtga agagtttatt gatgaagctg cgaaactttt tgcttttctc aactctatag ctcctttcag agctggtaat gagcctacac aacgaatatt ttttgaaaaa cttgctaagg ctgcaggtca tcaactagat ttttcagttg cgacagagaa acgtataatg cgtgcctgta ttgattcaat gactctagag ggtgatgggg ctcataaaga gatgaaactt ctgtttgaag atatctcttg tccccaaaaa ataattgttt taaaggattt tattcgacgc atacctagat tggagcgcga gcgtttgaat gatgaatatg ttataatgcc aaaggaaggt attacttata caggtattta taaaataggt actgtaaatt ccgttataat cgaaacagcg gattctcatg ttctatgcgt taaagattat tttccaccgg aacaacttaa gacattaaaa cctggtgatg tgtgtaccgt tacatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BaroA.17330.e.B1.PS01837
PURIFICATION DATe: 5/16/2013
CONCENTRATION: 27mg/ml
OBSERVED MW: 66kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 67
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV FSDISLARNY VYPHMKVLKN KHNITSTKKL AYECKRFAKK VMINLNNEPL PQEFDSSYLK YIHQRLFEST FEWAGYTRDL SFTFDDGTVA EMPMMKVPDS DIFYVQGKNI QESLKKFDQL LASKNNLQGL SREEFIDEAA KLFAFLNSIA PFRAGNEPTQ RIFFEKLAKA AGHQLDFSVA TEKRIMRACI DSMTLEGDGA HKEMKLLFED ISCPQKIIVL KDFIRRIPRL ERERLNDEYV IMPKEGITYT GIYKIGTVNS VIIETADSHV LCVKDYFPPE QLKTLKPGDV CTVTVXIDID QILIPAEKMA PLKEEEIIRK VSRTNCIKES QKXXXFXXXX XRETTTVXXX XXXXXXXXXX XXMXHXHHHH MTPLKTVEPF VIRVDNSTGI IETVSALKET PNNYDEAITR YFASKYVRAR EGFQLSEAEY NFRLISLLSS PEEQNRFAKW YSGNNPESPQ NIYHNMTAKV TIKSISFLSK DLIQVRYYKT IREXNGKENI SXXVSIXNFS YINAHISTED RLINPLGFQV SE
Validated NT Sequence
atggctcacc accaccacca ccatatggta ttttctgata tatctttagc tcgtaattat gtttatcctc acatgaaagt attaaagaat aaacataata taacgagtac taaaaaactt gcatacgaat gtaagcgttt tgcaaaaaaa gtaatgatca atttaaataa cgaaccattg ccccaagaat ttgattcctc ttacttaaaa tatatccatc aacgcttatt cgagagcaca tttgaatggg ctggatatac tcgtgatctt tcgtttacgt ttgatgatgg cactgttgct gaaatgccaa tgatgaaagt tccagactca gatatttttt atgtacaggg taagaacatt caagaaagct taaagaaatt tgatcaacta cttgcttcaa agaataattt gcaaggttta tctcgtgaag agtttattga tgaagctgcg aaactttttg cttttctcaa ctctatagct cctttcagag ctggtaatga gcctacacaa cgaatatttt ttgaaaaact tgctaaggct gcaggtcatc aactagattt ttcagttgcg acagagaaac gtataatgcg tgcctgtatt gattcaatga ctctagaggg tgatggggct cataaagaga tgaaacttct gtttgaagat atctcttgtc cccaaaaaat aattgtttta aaggatttta ttcgacgcat acctagattg gagcgcgagc gtttgaatga tgaatatgtt ataatgccaa aggaaggtat tacttataca ggtatttata aaataggtac tgtaaattcc gttataatcg aaacagcgga ttctcatgtt ctatgcgtta aagattattt tccaccggaa caacttaaga cattaaaacc tggtgatgtg tgtaccgtta cagttncaat agatatagat caaattctca ttccagcaga aaaaatggct cctttaaagg aagaagaaat aatcagaaaa gtttcacgta ctaattgtat taaagaaagc caaaagnnnn nnnattttnn tnnnnnntnn natagggaga ccacaacggt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatgn ctcaccncca ccaccaccat atgacaccnt taaaaacagt agagccnttt gttatccgcg ttgataattc aacaggtatt attgagacag taagtgcctt aaaagaaacg ccaaacaatt atgatgaagc aataacacgt tattttgcta gcaaatatgt tcgtgcgcgc gaaggttttc aattatcaga ggcggaatac aattttcgct tgatttctct tttatcttca ccagaagaac aaaaccgttt tgcaaaatgg tattcgggca acaatccaga aagtccgcaa aatatctatc acaacatgac tgctaaggtc acaatcaagt caatctcctt tttaagcaaa gatcttattc aagtgcgtta ctataaaacg atcagagaan ngaatggaaa agaaaatatt tcccannngg tctcgatcnt caatttttcc tacattaatg cacacatttc aacagaagac cgactaatca acccgcttgg ttttcaagtg tcagaata
Expressed Protein Sequence
MAHHHHHHMV FSDISLARNY VYPHMKVLKN KHNITSTKKL AYECKRFAKK VMINLNNEPL PQEFDSSYLK YIHQRLFEST FEWAGYTRDL SFTFDDGTVA EMPMMKVPDS DIFYVQGKNI QESLKKFDQL LASKNNLQGL SREEFIDEAA KLFAFLNSIA PFRAGNEPTQ RIFFEKLAKA AGHQLDFSVA TEKRIMRACI DSMTLEGDGA HKEMKLLFED ISCPQKIIVL KDFIRRIPRL ERERLNDEYV IMPKEGITYT GIYKIGTVNS VIIETADSHV LCVKDYFPPE QLKTLKPGDV CTVTVPIDID QILIPAEKMA PLKEEEIIRK VSRTNCIKES QKEIDYLLKF VYRDITVLDE QIMCIRKCPE TSKQLSEQIR KSPQAFGKLA GFKVFGIRSP KRRIAERNIE KLTSEIEKHG EIVKNVKDEI SLEFEKDQKR VGQVVKKASK KIQDILNLPK DEQKNALEAS PLLYEELRSF LNEVNSRLSA REKDYICDYH DKKLAESIGV SEMHAMTIIK TVNKSAKLCK QFEKIKEIKK VDCSEIMAMA V
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTAT TTTCTGATAT ATCTTTAGCT CGTAATTATG TTTATCCTCA CATGAAAGTA TTAAAGAATA AACATAATAT AACGAGTACT AAAAAACTTG CATACGAATG TAAGCGTTTT GCAAAAAAAG TAATGATCAA TTTAAATAAC GAACCATTGC CCCAAGAATT TGATTCCTCT TACTTAAAAT ATATCCATCA ACGCTTATTC GAGAGCACAT TTGAATGGGC TGGATATACT CGTGATCTTT CGTTTACGTT TGATGATGGC ACTGTTGCTG AAATGCCAAT GATGAAAGTT CCAGACTCAG ATATTTTTTA TGTACAGGGT AAGAACATTC AAGAAAGCTT AAAGAAATTT GATCAACTAC TTGCTTCAAA GAATAATTTG CAAGGTTTAT CTCGTGAAGA GTTTATTGAT GAAGCTGCGA AACTTTTTGC TTTTCTCAAC TCTATAGCTC CTTTCAGAGC TGGTAATGAG CCTACACAAC GAATATTTTT TGAAAAACTT GCTAAGGCTG CAGGTCATCA ACTAGATTTT TCAGTTGCGA CAGAGAAACG TATAATGCGT GCCTGTATTG ATTCAATGAC TCTAGAGGGT GATGGGGCTC ATAAAGAGAT GAAACTTCTG TTTGAAGATA TCTCTTGTCC CCAAAAAATA ATTGTTTTAA AGGATTTTAT TCGACGCATA CCTAGATTGG AGCGCGAGCG TTTGAATGAT GAATATGTTA TAATGCCAAA GGAAGGTATT ACTTATACAG GTATTTATAA AATAGGTACT GTAAATTCCG TTATAATCGA AACAGCGGAT TCTCATGTTC TATGCGTTAA AGATTATTTT CCACCGGAAC AACTTAAGAC ATTAAAACCT GGTGATGTGT GTACCGTTAC AGTTCCAATA GATATAGATC AAATTCTCAT TCCAGCAGAA AAAATGGCTC CTTTAAAGGA AGAAGAAATA ATCAGAAAAG TTTCACGTAC TAATTGTATT AAAGAAAGCC AAAAGGAAAT TGATTATCTT TTAAAGTTTG TTTATAGAGA CATAACAGTA TTAGACGAAC AGATAATGTG TATTCGTAAA TGTCCTGAAA CAAGTAAACA GCTTTCCGAA CAGATTAGAA AATCTCCTCA AGCTTTTGGT AAGCTAGCTG GTTTTAAAGT ATTTGGTATA AGGAGTCCAA AACGTAGGAT AGCTGAAAGA AATATTGAAA AACTCACTTC GGAGATTGAA AAACATGGGG AAATTGTGAA AAATGTTAAA GATGAAATTT CTCTAGAATT TGAAAAAGAT CAAAAGCGTG TTGGGCAAGT GGTGAAAAAA GCTAGTAAAA AAATACAAGA TATTCTTAAT TTGCCAAAGG ATGAGCAAAA AAATGCCTTA GAGGCCTCTC CTCTTCTTTA CGAAGAGCTT CGCAGCTTTC TGAATGAAGT CAATTCTCGT CTTTCAGCAA GAGAGAAAGA TTACATCTGT GACTATCATG ATAAAAAACT TGCCGAAAGC ATTGGTGTAT CAGAAATGCA CGCAATGACA ATTATAAAAA CTGTTAACAA AAGCGCAAAA TTGTGTAAAC AATTTGAAAA GATCAAAGAA ATTAAAAAAG TTGATTGTTC AGAAATAATG GCTATGGCCG TTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT
Details for BaroA.17330.e.B1.PS01838
PURIFICATION DATe: 5/16/2013
CONCENTRATION: 17mg/ml
OBSERVED MW: 66kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 67
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV FSDISLARNY VYPHMKVLKN KHNITSTKKL AYECKRFAKK VMINLNNEPL PQEFDSSYLK YIHQRLFEST FEWAGYTRDL SFTFDDGTVA EMPMMKVPDS DIFYVQGKNI QESLKKFDQL LASKNNLQGL SREEFIDEAA KLFAFLNSIA PFRAGNEPTQ RIFFEKLAKA AGHQLDFSVA TEKRIMRACI DSMTLEGDGA HKEMKLLFED ISCPQKIIVL KDFIRRIPRL ERERLNDEYV IMPKEGITYT GIYKIGTVNS VIIETADSHV LCVKDYFPPE QLKTLKPGDV CTVTVXIDID QILIPAEKMA PLKEEEIIRK VSRTNCIKES QKXXXFXXXX XRETTTVXXX XXXXXXXXXX XXMXHXHHHH MTPLKTVEPF VIRVDNSTGI IETVSALKET PNNYDEAITR YFASKYVRAR EGFQLSEAEY NFRLISLLSS PEEQNRFAKW YSGNNPESPQ NIYHNMTAKV TIKSISFLSK DLIQVRYYKT IREXNGKENI SXXVSIXNFS YINAHISTED RLINPLGFQV SE
Validated NT Sequence
atggctcacc accaccacca ccatatggta ttttctgata tatctttagc tcgtaattat gtttatcctc acatgaaagt attaaagaat aaacataata taacgagtac taaaaaactt gcatacgaat gtaagcgttt tgcaaaaaaa gtaatgatca atttaaataa cgaaccattg ccccaagaat ttgattcctc ttacttaaaa tatatccatc aacgcttatt cgagagcaca tttgaatggg ctggatatac tcgtgatctt tcgtttacgt ttgatgatgg cactgttgct gaaatgccaa tgatgaaagt tccagactca gatatttttt atgtacaggg taagaacatt caagaaagct taaagaaatt tgatcaacta cttgcttcaa agaataattt gcaaggttta tctcgtgaag agtttattga tgaagctgcg aaactttttg cttttctcaa ctctatagct cctttcagag ctggtaatga gcctacacaa cgaatatttt ttgaaaaact tgctaaggct gcaggtcatc aactagattt ttcagttgcg acagagaaac gtataatgcg tgcctgtatt gattcaatga ctctagaggg tgatggggct cataaagaga tgaaacttct gtttgaagat atctcttgtc cccaaaaaat aattgtttta aaggatttta ttcgacgcat acctagattg gagcgcgagc gtttgaatga tgaatatgtt ataatgccaa aggaaggtat tacttataca ggtatttata aaataggtac tgtaaattcc gttataatcg aaacagcgga ttctcatgtt ctatgcgtta aagattattt tccaccggaa caacttaaga cattaaaacc tggtgatgtg tgtaccgtta cagttncaat agatatagat caaattctca ttccagcaga aaaaatggct cctttaaagg aagaagaaat aatcagaaaa gtttcacgta ctaattgtat taaagaaagc caaaagnnnn nnnattttnn tnnnnnntnn natagggaga ccacaacggt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatgn ctcaccncca ccaccaccat atgacaccnt taaaaacagt agagccnttt gttatccgcg ttgataattc aacaggtatt attgagacag taagtgcctt aaaagaaacg ccaaacaatt atgatgaagc aataacacgt tattttgcta gcaaatatgt tcgtgcgcgc gaaggttttc aattatcaga ggcggaatac aattttcgct tgatttctct tttatcttca ccagaagaac aaaaccgttt tgcaaaatgg tattcgggca acaatccaga aagtccgcaa aatatctatc acaacatgac tgctaaggtc acaatcaagt caatctcctt tttaagcaaa gatcttattc aagtgcgtta ctataaaacg atcagagaan ngaatggaaa agaaaatatt tcccannngg tctcgatcnt caatttttcc tacattaatg cacacatttc aacagaagac cgactaatca acccgcttgg ttttcaagtg tcagaata
Expressed Protein Sequence
MAHHHHHHMV FSDISLARNY VYPHMKVLKN KHNITSTKKL AYECKRFAKK VMINLNNEPL PQEFDSSYLK YIHQRLFEST FEWAGYTRDL SFTFDDGTVA EMPMMKVPDS DIFYVQGKNI QESLKKFDQL LASKNNLQGL SREEFIDEAA KLFAFLNSIA PFRAGNEPTQ RIFFEKLAKA AGHQLDFSVA TEKRIMRACI DSMTLEGDGA HKEMKLLFED ISCPQKIIVL KDFIRRIPRL ERERLNDEYV IMPKEGITYT GIYKIGTVNS VIIETADSHV LCVKDYFPPE QLKTLKPGDV CTVTVPIDID QILIPAEKMA PLKEEEIIRK VSRTNCIKES QKEIDYLLKF VYRDITVLDE QIMCIRKCPE TSKQLSEQIR KSPQAFGKLA GFKVFGIRSP KRRIAERNIE KLTSEIEKHG EIVKNVKDEI SLEFEKDQKR VGQVVKKASK KIQDILNLPK DEQKNALEAS PLLYEELRSF LNEVNSRLSA REKDYICDYH DKKLAESIGV SEMHAMTIIK TVNKSAKLCK QFEKIKEIKK VDCSEIMAMA V
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTAT TTTCTGATAT ATCTTTAGCT CGTAATTATG TTTATCCTCA CATGAAAGTA TTAAAGAATA AACATAATAT AACGAGTACT AAAAAACTTG CATACGAATG TAAGCGTTTT GCAAAAAAAG TAATGATCAA TTTAAATAAC GAACCATTGC CCCAAGAATT TGATTCCTCT TACTTAAAAT ATATCCATCA ACGCTTATTC GAGAGCACAT TTGAATGGGC TGGATATACT CGTGATCTTT CGTTTACGTT TGATGATGGC ACTGTTGCTG AAATGCCAAT GATGAAAGTT CCAGACTCAG ATATTTTTTA TGTACAGGGT AAGAACATTC AAGAAAGCTT AAAGAAATTT GATCAACTAC TTGCTTCAAA GAATAATTTG CAAGGTTTAT CTCGTGAAGA GTTTATTGAT GAAGCTGCGA AACTTTTTGC TTTTCTCAAC TCTATAGCTC CTTTCAGAGC TGGTAATGAG CCTACACAAC GAATATTTTT TGAAAAACTT GCTAAGGCTG CAGGTCATCA ACTAGATTTT TCAGTTGCGA CAGAGAAACG TATAATGCGT GCCTGTATTG ATTCAATGAC TCTAGAGGGT GATGGGGCTC ATAAAGAGAT GAAACTTCTG TTTGAAGATA TCTCTTGTCC CCAAAAAATA ATTGTTTTAA AGGATTTTAT TCGACGCATA CCTAGATTGG AGCGCGAGCG TTTGAATGAT GAATATGTTA TAATGCCAAA GGAAGGTATT ACTTATACAG GTATTTATAA AATAGGTACT GTAAATTCCG TTATAATCGA AACAGCGGAT TCTCATGTTC TATGCGTTAA AGATTATTTT CCACCGGAAC AACTTAAGAC ATTAAAACCT GGTGATGTGT GTACCGTTAC AGTTCCAATA GATATAGATC AAATTCTCAT TCCAGCAGAA AAAATGGCTC CTTTAAAGGA AGAAGAAATA ATCAGAAAAG TTTCACGTAC TAATTGTATT AAAGAAAGCC AAAAGGAAAT TGATTATCTT TTAAAGTTTG TTTATAGAGA CATAACAGTA TTAGACGAAC AGATAATGTG TATTCGTAAA TGTCCTGAAA CAAGTAAACA GCTTTCCGAA CAGATTAGAA AATCTCCTCA AGCTTTTGGT AAGCTAGCTG GTTTTAAAGT ATTTGGTATA AGGAGTCCAA AACGTAGGAT AGCTGAAAGA AATATTGAAA AACTCACTTC GGAGATTGAA AAACATGGGG AAATTGTGAA AAATGTTAAA GATGAAATTT CTCTAGAATT TGAAAAAGAT CAAAAGCGTG TTGGGCAAGT GGTGAAAAAA GCTAGTAAAA AAATACAAGA TATTCTTAAT TTGCCAAAGG ATGAGCAAAA AAATGCCTTA GAGGCCTCTC CTCTTCTTTA CGAAGAGCTT CGCAGCTTTC TGAATGAAGT CAATTCTCGT CTTTCAGCAA GAGAGAAAGA TTACATCTGT GACTATCATG ATAAAAAACT TGCCGAAAGC ATTGGTGTAT CAGAAATGCA CGCAATGACA ATTATAAAAA CTGTTAACAA AAGCGCAAAA TTGTGTAAAC AATTTGAAAA GATCAAAGAA ATTAAAAAAG TTGATTGTTC AGAAATAATG GCTATGGCCG TTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT
Details for BaroA.17330.e.B2.PW37281
PURIFICATION DATe: 4/29/2013
CONCENTRATION: 30mg/ml
OBSERVED MW: 28kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN YVYPHMKVLK NKHNITSTKK LAYECKRFAK KVMINLNNEP LPQEFDSSYL KYIHQRLFES TFEWAGYTRD LSFTFDDGTV AEMPMMKVPD SDIFYVQGKN IQESLKKFDQ LLASKNNLQG LSREEFIDEA AKLFAFLNSI APFRAGNEPT QRIFFEKLAK AAGHQLDFSV ATEKRIMRAC IDSMTLEGDG AHKEMKLLFE DISCPQKIIV LKDFIRRIPR LERERLND
Validated NT Sequence
atcttactca cttaatcatt caaacgctcg cgctccaatc taggtatgcg tcgaataaaa tcctttaaaa caattatttt ttggggacaa gagatatctt caaacagaag tttcatctct ttatgagccc catcaccctc tagagtcatt gaatcaatac aggcacgcat tatacgtttc tctgtcgcaa ctgaaaaatc tagttgatga cctgcagcct tagcaagttt ttcaaaaaat attcgttgtg taggctcatt accagctctg aaaggagcta tagagttgag aaaagcaaaa agtttcgcag cttcatcaat aaactcttca cgagataaac cttgcaaatt attctttgaa gcaagtagtt gatcaaattt ctttaagctt tcttgaatgt tcttaccctg tacataaaaa atatctgagt ctggaacttt catcattggc atttcagcaa cagtgccatc atcaaacgta aacgaaagat cacgagtata tccagcccat tcaaatgtgc tctcgaataa gcgttgatgg atatatttta agtaagagga atcaaattct tggggcaatg gttcgttatt taaattgatc attacttttt ttgcaaaacg cttacattcg tatgcaagtt ttttagtact cgttatatta tgtttattct ttaatacttt catgtgagga taaacataat tcatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMN YVYPHMKVLK NKHNITSTKK LAYECKRFAK KVMINLNNEP LPQEFDSSYL KYIHQRLFES TFEWAGYTRD LSFTFDDGTV AEMPMMKVPD SDIFYVQGKN IQESLKKFDQ LLASKNNLQG LSREEFIDEA AKLFAFLNSI APFRAGNEPT QRIFFEKLAK AAGHQLDFSV ATEKRIMRAC IDSMTLEGDG AHKEMKLLFE DISCPQKIIV LKDFIRRIPR LERERLND
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATT ATGTTTATCC TCACATGAAA GTATTAAAGA ATAAACATAA TATAACGAGT ACTAAAAAAC TTGCATACGA ATGTAAGCGT TTTGCAAAAA AAGTAATGAT CAATTTAAAT AACGAACCAT TGCCCCAAGA ATTTGATTCC TCTTACTTAA AATATATCCA TCAACGCTTA TTCGAGAGCA CATTTGAATG GGCTGGATAT ACTCGTGATC TTTCGTTTAC GTTTGATGAT GGCACTGTTG CTGAAATGCC AATGATGAAA GTTCCAGACT CAGATATTTT TTATGTACAG GGTAAGAACA TTCAAGAAAG CTTAAAGAAA TTTGATCAAC TACTTGCTTC AAAGAATAAT TTGCAAGGTT TATCTCGTGA AGAGTTTATT GATGAAGCTG CGAAACTTTT TGCTTTTCTC AACTCTATAG CTCCTTTCAG AGCTGGTAAT GAGCCTACAC AACGAATATT TTTTGAAAAA CTTGCTAAGG CTGCAGGTCA TCAACTAGAT TTTTCAGTTG CGACAGAGAA ACGTATAATG CGTGCCTGTA TTGATTCAAT GACTCTAGAG GGTGATGGGG CTCATAAAGA GATGAAACTT CTGTTTGAAG ATATCTCTTG TCCCCAAAAA ATAATTGTTT TAAAGGATTT TATTCGACGC ATACCTAGAT TGGAGCGCGA GCGTTTGAAT GATTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT