BaroA.17330.b

Bep4 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaroA.17330.b
ORGANISM: Bartonella rochalimae ATCC BAA-1498
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaroA.17330.b.B2.GE37267 BID 387 531
BaroA.17330.b.B3.GE37441 FIC 85 346
BaroA.17330.b.B4.GE37442 N-term extended FIC 1 346
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaroA.17330.b.B2.PS01970 BID 387 531

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|685782.3.peg.1279
RefSeq: WP_035007281.1
UniProt: E6YLB1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFKSKKKIIS NYKTDYATPR CLIQKRREVV NSVISAHMIE GYTFNSKTLG ILEEYVNGDY SLQQLNSQLD NSMKKLIKVK AQKEHSSYSE KKLINKYGIL VNKCGITDTV ALRDKIAHDV AKAIVNLSYE KVPEQFDTSY LKRLHKSLFG NIFDWAGKTR DELKFSDSRI NAKLSLMGEL SSINNDEIQD SEEIQESLQK IDNILFEKNN LRGLSSEEFA HNVARMFSFL NYVCPFVGGN ECTQKVFFEQ LANAVGYKLD FSVVTEERMK FVSHASETLE GDIYALTPME HLFEDISDPN KVCMLKEFVS NNKYKGVEKQ IIVVARKDVV YNGIYRGRSS NSVLVEIPDC CVVCHKDCFM PEELQALKLG DTVAFKAVIP KVFEDVLIPK RVCVPLTREE IAEKVKKDFS VQRSWKDVEY YLKLVYNNPA IFKKDIELID KFSCFDKALA RQFAKQLAEK FMESPQSISR LAGLEVLGIR NLKRKEAKAS CAALSDAIVR YADIVISARD RILEQQEIQI GISSRSVKMP SKEMQDILNL PEDTRKEMLE VSLSLRQECF DFMHGIMSRL SLNEYKALDD NNYAELAESL GVSESKAKQI ITIVQPIEKL YLDLKKTLSV SLSENTAIVQ AN
NT Sequence
atgttcaaga gcaaaaaaaa gataatttca aattataaga cggattatgc tacgcctcgg tgcctaatac aaaagcgtcg tgaagtagta aattcagtga ttagtgcgca tatgattgag ggatatactt ttaactctaa aactttaggt attttagaag aatatgtgaa cggggattat tcactacagc agcttaatag tcagttggat aattctatga aaaaactcat taaagtaaag gcacaaaagg aacattcaag ctattctgaa aaaaagctga taaataaata tggaattctg gtaaataaat gtggaataac agatacagtt gctcttcgtg acaaaattgc acacgatgtc gcaaaagcaa ttgttaattt gtcttatgaa aaggtaccag aacaatttga cacttcttat ctaaaacgtc ttcataagag cttattcggg aatatttttg attgggctgg aaagactcgt gacgagctta aattttctga tagtagaata aatgctaagt tgtcattaat gggagaattg agctctataa ataatgatga aattcaagat agtgaggaaa ttcaggaaag tttgcaaaaa attgataaca ttctttttga gaagaataat ctaagaggtt tatcatctga agaatttgct cataatgtag caagaatgtt ttcttttctt aattatgtat gtccttttgt aggggggaat gaatgtacgc aaaaggtgtt ttttgaacaa cttgctaacg ctgttggtta taaactcgat ttttcggttg taacagaaga gcgcatgaaa tttgttagcc atgcttcgga aacattagaa ggtgatatat acgctttaac tcctatggag catttgtttg aagatatttc tgatccaaac aaagtgtgta tgctaaaaga gtttgttagc aataacaagt ataaaggtgt agaaaaacag attattgtag ttgctcgtaa agatgtggtt tataacggta tttatagagg ccgtagttca aattcggttc tagtcgaaat accagattgc tgtgtcgtgt gccacaaaga ttgttttatg ccagaagaat tacaagcctt aaaattaggt gatacagttg cttttaaagc tgtaatacct aaggtttttg aagatgttct cattccaaaa agagtatgtg ttcctttgac aagagaagaa attgctgaga aagttaaaaa agatttctct gttcaaagaa gctggaaaga tgttgagtat tatttaaaac ttgtttataa taatccggca atctttaaaa aagatatcga attaattgat aaattttcat gttttgataa agcgcttgca agacagtttg caaaacagct tgcagaaaag tttatggaat ctcctcaatc tatttctcgc cttgcaggtt tagaggtgtt aggtatcagg aatttaaagc gaaaagaagc taaagcgtct tgtgctgcac ttagtgatgc aattgttcgg tatgcagata ttgtaatatc ggctagagat cgaattttag aacaacaaga aatacaaatt ggtatttcaa gtagatctgt aaaaatgccc agtaaggaaa tgcaagatat tttaaatttg ccagaagaca cgcgaaaaga aatgcttgag gtttctcttt cattacgtca ggaatgtttt gattttatgc atggaatcat gagtcgtctt tcattgaatg agtataaagc acttgatgat aataattatg cagagcttgc tgaaagtttg ggggtatcag agagtaaagc aaagcaaatt ataacaatcg ttcaaccgat tgaaaaattg tatttggatc ttaaaaaaac tctctccgtt agtctttcgg aaaatacagc tatagtgcaa gccaattaa
Details for BaroA.17330.b.B2.GE37267
HARVESTED ON: 8/28/2013
SEQUENCED ON: 9/3/2013
EXPECTED MW: 18kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML IPKRVCVPLT REEIAEKVKK DFSVQRSWKD VEYYLKLVYN NPAIFKKDIE LIDKFSCFDK ALARQFAKQL AEKFMESPQS ISRLAGLEVL GIRNLKRKEA KASCAALSDA IVRYADIVIS ARDRILEQQE IQIGISSRSV KMPS
Validated NT Sequence
anattttgtn nnntttaaga aggaganata ccatggctca ccaccaccac caccatatgc tcattccaaa aagagtatgt gttcctttga caagagaaga aattgctgag aaagttaaaa aagatttctc tgttcaaaga agctggaaag atgttgagta ttatttaaaa cttgtttata ataatccggc aatctttaaa aaagatatcg aattaattga taaattttca tgttttgata aagcgcttgc aagacagttt gcaaaacagc ttgcagaaaa gtttatggaa tctcctcaat ctatttctcg ccttgcaggt ttagaggtgt taggtatcag gaatttaaag cgaaaagaag ctaaagcgtc ttgtgctgca cttagtgatg caattgttcg gtatgcagat attgtaatat cggctagaga tcgaatttta gaacaacaag aaatacaaat tggtatttca agtagatctg taaaaatgcc cagttaagtg agtaagatag gatccggctg ctaacaannn naaangannt nnannnnnnn nnngncnnng ncnttncnnn ncnnnnngnn ncnngaccnc ngnttgtnnn nntcnnnnac annncnntcc cnacnnanan cnntnnnnnn nnnncnnnnn nnacnnnnnn nnnnnnnnnn nnnnnncacn tnnccannnn nnnagcngnn nnnncngaan nnnccnnnnn nnnncgtnnn nnnnnnnnnn nnnnncnatg anngnnnctt nnnnnnannn nnnnnag
Expected Protein Sequence
MAHHHHHHLI PKRVCVPLTR EEIAEKVKKD FSVQRSWKDV EYYLKLVYNN PAIFKKDIEL IDKFSCFDKA LARQFAKQLA EKFMESPQSI SRLAGLEVLG IRNLKRKEAK ASCAALSDAI VRYADIVISA RDRILEQQEI QIGISSRSVK MPS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctcattc caaaaagagt atgtgttcct ttgacaagag aagaaattgc tgagaaagtt aaaaaagatt tctctgttca aagaagctgg aaagatgttg agtattattt aaaacttgtt tataataatc cggcaatctt taaaaaagat atcgaattaa ttgataaatt ttcatgtttt gataaagcgc ttgcaagaca gtttgcaaaa cagcttgcag aaaagtttat ggaatctcct caatctattt ctcgccttgc aggtttagag gtgttaggta tcaggaattt aaagcgaaaa gaagctaaag cgtcttgtgc tgcacttagt gatgcaattg ttcggtatgc agatattgta atatcggcta gagatcgaat tttagaacaa caagaaatac aaattggtat ttcaagtaga tctgtaaaaa tgcccagttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BaroA.17330.b.B3.GE37441
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMH SSYSEKKLIN KYGILVNKCG ITDTVALRDK IAHDVAKAIV NLSYEKVPEQ FDTSYLKRLH KSLFGNIFDW AGKTRDELKF SDSRINAKLS LMGELSSINN DEIQDSEEIQ ESLQKIDNIL FEKNNLRGLS SEEFAHNVAR MFSFLNYVCP FVGGNECTQK VFFEQLANAV GYKLDFSVVT EERMKFVSHA SETLEGDIYA LTPMEHLFED ISDPNKVCML KEFVSNNKYK GVEKQIIVVA RKDVVYNGIY RGRSSNSVLV X
Validated NT Sequence
ttttntttna ctttaagaag gagnnatacc atggctcacc accaccacca ccatatgcat tcaagctatt ctgaaaaaaa gctgataaat aaatatggaa ttctggtaaa taaatgtgga ataacagata cagttgctct tcgtgacaaa attgcacacg atgtcgcaaa agcaattgtt aatttgtctt atgaaaaggt accagaacaa tttgacactt cttatctaaa acgtcttcat aagagcttat tcgggaatat ttttgattgg gctggaaaga ctcgtgacga gcttaaattt tctgatagta gaataaatgc taagttgtca ttaatgggag aattgagctc tataaataat gatgaaattc aagatagtga ggaaattcag gaaagtttgc aaaaaattga taacattctt tttgagaaga ataatctaag aggtttatca tctgaagaat ttgctcataa tgtagcaaga atgttttctt ttcttaatta tgtatgtcct tttgtagggg ggaatgaatg tacgcaaaag gtgttttttg aacaacttgc taacgctgtt ggttataaac tcgatttttc ggttgtaaca gaagagcgca tgaaatttgt tagccatgct tcggaaacat tagaaggtga tatatacgct ttaactccta tggagcattt gtttgaagat atttctgatc caaacaaagt gtgtatgcta aaagagtttg ttagcaataa caagtataaa ggtgtagaaa aacagattat tgtagttgct cgtaaagatg tggtttataa cggtatttat agaggccgta gttcaaattc ggttctagtc naataagtga gtaaganagg atccggctgc tanannnccc cnnaangnnn nnnnnnnaan nnanaaannn nnntnnactc ctannnacnn tnngntnnnn gatnntnncn nanccnnncn antnnnnnng ctnnnttngt nagcnnnanc nnntnntnnn ngntnnnnaa aancnnagta ntcnnnccnn nnnnnnnngg ntnnnnnnnn nntnnnnnng gccncnantn nnngtncngc nnannnnnnn nnnnnnnnnn nnnnnnnnnc
Expected Protein Sequence
MAHHHHHHHS SYSEKKLINK YGILVNKCGI TDTVALRDKI AHDVAKAIVN LSYEKVPEQF DTSYLKRLHK SLFGNIFDWA GKTRDELKFS DSRINAKLSL MGELSSINND EIQDSEEIQE SLQKIDNILF EKNNLRGLSS EEFAHNVARM FSFLNYVCPF VGGNECTQKV FFEQLANAVG YKLDFSVVTE ERMKFVSHAS ETLEGDIYAL TPMEHLFEDI SDPNKVCMLK EFVSNNKYKG VEKQIIVVAR KDVVYNGIYR GRSSNSVLVE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcattcaa gctattctga aaaaaagctg ataaataaat atggaattct ggtaaataaa tgtggaataa cagatacagt tgctcttcgt gacaaaattg cacacgatgt cgcaaaagca attgttaatt tgtcttatga aaaggtacca gaacaatttg acacttctta tctaaaacgt cttcataaga gcttattcgg gaatattttt gattgggctg gaaagactcg tgacgagctt aaattttctg atagtagaat aaatgctaag ttgtcattaa tgggagaatt gagctctata aataatgatg aaattcaaga tagtgaggaa attcaggaaa gtttgcaaaa aattgataac attctttttg agaagaataa tctaagaggt ttatcatctg aagaatttgc tcataatgta gcaagaatgt tttcttttct taattatgta tgtccttttg taggggggaa tgaatgtacg caaaaggtgt tttttgaaca acttgctaac gctgttggtt ataaactcga tttttcggtt gtaacagaag agcgcatgaa atttgttagc catgcttcgg aaacattaga aggtgatata tacgctttaa ctcctatgga gcatttgttt gaagatattt ctgatccaaa caaagtgtgt atgctaaaag agtttgttag caataacaag tataaaggtg tagaaaaaca gattattgta gttgctcgta aagatgtggt ttataacggt atttatagag gccgtagttc aaattcggtt ctagtcgaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BaroA.17330.b.B4.GE37442
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 93
Validated AA Sequence
MAHHHHHHMF KSKKKIISNY KTDYATPRCL IQKRREVVNS VISAHMIEGY TFNSKTLGIL EEYVNGDYSL QQLNSQLDNS MKKLIKVKAQ KEHSSYSEKK LINKYGILVN KCGITDTVAL RDKIAHDVAK AIVNLSYEKV PEQFDTSYLK RLHKSLFGNI FDWAGKTRDE LKFSDSRINA KLSLMGELSS INNDEIQDSE EIQESLQKID NILFEKNNLR GLSSEEFAHN VARMFSFLNY VCPFVGGNEC TQKVFFEQLA NAVGYKLDFS VVTEERMKFV SHASETLEXX IYALTPMEHL FEXXSXPNKV XMLKEFVSXN XYXXVXNXXL
Validated NT Sequence
attttgttta actttaagaa ggagnnatac catggctcac caccaccacc accatatgtt caagagcaaa aaaaagataa tttcaaatta taagacggat tatgctacgc ctcggtgcct aatacaaaag cgtcgtgaag tagtaaattc agtgattagt gcgcatatga ttgagggata tacttttaac tctaaaactt taggtatttt agaagaatat gtgaacgggg attattcact acagcagctt aatagtcagt tggataattc tatgaaaaaa ctcattaaag taaaggcaca aaaggaacat tcaagctatt ctgaaaaaaa gctgataaat aaatatggaa ttctggtaaa taaatgtgga ataacagata cagttgctct tcgtgacaaa attgcacacg atgtcgcaaa agcaattgtt aatttgtctt atgaaaaggt accagaacaa tttgacactt cttatctaaa acgtcttcat aagagcttat tcgggaatat ttttgattgg gctggaaaga ctcgtgacga gcttaaattt tctgatagta gaataaatgc taagttgtca ttaatgggag aattgagctc tataaataat gatgaaattc aagatagtga ggaaattcag gaaagtttgc aaaaaattga taacattctt tttgagaaga ataatctaag aggtttatca tctgaagaat ttgctcataa tgtagcaaga atgttttctt ttcttaatta tgtatgtcct tttgtagggg ggaatgaatg tacgcaaaag gtgttttttg aacaacttgc taacgctgtt ggttataaac tcgatttttc ggttgtaaca gaagagcgca tgaaatttgt tagccatgct tcggaaacat tagaannnga natatacgct ttaactccta tggagcattt gtttgaanat ntttctnanc caaacaaagt gngnatgcta aaagagtttg ttagcannaa cnagtatnan nnngtanaaa acanantatt gtagttcgta aanatgtngt tnannacggn ntttnnnnna ngccgtantt caantnggtt ctagtcnana ntnannnnnn nnnnannnnn nnnnnnnnnn nnna
Expected Protein Sequence
MAHHHHHHMF KSKKKIISNY KTDYATPRCL IQKRREVVNS VISAHMIEGY TFNSKTLGIL EEYVNGDYSL QQLNSQLDNS MKKLIKVKAQ KEHSSYSEKK LINKYGILVN KCGITDTVAL RDKIAHDVAK AIVNLSYEKV PEQFDTSYLK RLHKSLFGNI FDWAGKTRDE LKFSDSRINA KLSLMGELSS INNDEIQDSE EIQESLQKID NILFEKNNLR GLSSEEFAHN VARMFSFLNY VCPFVGGNEC TQKVFFEQLA NAVGYKLDFS VVTEERMKFV SHASETLEGD IYALTPMEHL FEDISDPNKV CMLKEFVSNN KYKGVEKQII VVARKDVVYN GIYRGRSSNS VLVE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttca agagcaaaaa aaagataatt tcaaattata agacggatta tgctacgcct cggtgcctaa tacaaaagcg tcgtgaagta gtaaattcag tgattagtgc gcatatgatt gagggatata cttttaactc taaaacttta ggtattttag aagaatatgt gaacggggat tattcactac agcagcttaa tagtcagttg gataattcta tgaaaaaact cattaaagta aaggcacaaa aggaacattc aagctattct gaaaaaaagc tgataaataa atatggaatt ctggtaaata aatgtggaat aacagataca gttgctcttc gtgacaaaat tgcacacgat gtcgcaaaag caattgttaa tttgtcttat gaaaaggtac cagaacaatt tgacacttct tatctaaaac gtcttcataa gagcttattc gggaatattt ttgattgggc tggaaagact cgtgacgagc ttaaattttc tgatagtaga ataaatgcta agttgtcatt aatgggagaa ttgagctcta taaataatga tgaaattcaa gatagtgagg aaattcagga aagtttgcaa aaaattgata acattctttt tgagaagaat aatctaagag gtttatcatc tgaagaattt gctcataatg tagcaagaat gttttctttt cttaattatg tatgtccttt tgtagggggg aatgaatgta cgcaaaaggt gttttttgaa caacttgcta acgctgttgg ttataaactc gatttttcgg ttgtaacaga agagcgcatg aaatttgtta gccatgcttc ggaaacatta gaaggtgata tatacgcttt aactcctatg gagcatttgt ttgaagatat ttctgatcca aacaaagtgt gtatgctaaa agagtttgtt agcaataaca agtataaagg tgtagaaaaa cagattattg tagttgctcg taaagatgtg gtttataacg gtatttatag aggccgtagt tcaaattcgg ttctagtcga atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BaroA.17330.b.B2.PS01970
PURIFICATION DATe: 1/22/2014
CONCENTRATION: 15mg/ml
OBSERVED MW: 16kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML IPKRVCVPLT REEIAEKVKK DFSVQRSWKD VEYYLKLVYN NPAIFKKDIE LIDKFSCFDK ALARQFAKQL AEKFMESPQS ISRLAGLEVL GIRNLKRKEA KASCAALSDA IVRYADIVIS ARDRILEQQE IQIGISSRSV KMPS
Validated NT Sequence
anattttgtn nnntttaaga aggaganata ccatggctca ccaccaccac caccatatgc tcattccaaa aagagtatgt gttcctttga caagagaaga aattgctgag aaagttaaaa aagatttctc tgttcaaaga agctggaaag atgttgagta ttatttaaaa cttgtttata ataatccggc aatctttaaa aaagatatcg aattaattga taaattttca tgttttgata aagcgcttgc aagacagttt gcaaaacagc ttgcagaaaa gtttatggaa tctcctcaat ctatttctcg ccttgcaggt ttagaggtgt taggtatcag gaatttaaag cgaaaagaag ctaaagcgtc ttgtgctgca cttagtgatg caattgttcg gtatgcagat attgtaatat cggctagaga tcgaatttta gaacaacaag aaatacaaat tggtatttca agtagatctg taaaaatgcc cagttaagtg agtaagatag gatccggctg ctaacaannn naaangannt nnannnnnnn nnngncnnng ncnttncnnn ncnnnnngnn ncnngaccnc ngnttgtnnn nntcnnnnac annncnntcc cnacnnanan cnntnnnnnn nnnncnnnnn nnacnnnnnn nnnnnnnnnn nnnnnncacn tnnccannnn nnnagcngnn nnnncngaan nnnccnnnnn nnnncgtnnn nnnnnnnnnn nnnnncnatg anngnnnctt nnnnnnannn nnnnnag
Expressed Protein Sequence
MAHHHHHHLI PKRVCVPLTR EEIAEKVKKD FSVQRSWKDV EYYLKLVYNN PAIFKKDIEL IDKFSCFDKA LARQFAKQLA EKFMESPQSI SRLAGLEVLG IRNLKRKEAK ASCAALSDAI VRYADIVISA RDRILEQQEI QIGISSRSVK MPS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTCATTC CAAAAAGAGT ATGTGTTCCT TTGACAAGAG AAGAAATTGC TGAGAAAGTT AAAAAAGATT TCTCTGTTCA AAGAAGCTGG AAAGATGTTG AGTATTATTT AAAACTTGTT TATAATAATC CGGCAATCTT TAAAAAAGAT ATCGAATTAA TTGATAAATT TTCATGTTTT GATAAAGCGC TTGCAAGACA GTTTGCAAAA CAGCTTGCAG AAAAGTTTAT GGAATCTCCT CAATCTATTT CTCGCCTTGC AGGTTTAGAG GTGTTAGGTA TCAGGAATTT AAAGCGAAAA GAAGCTAAAG CGTCTTGTGC TGCACTTAGT GATGCAATTG TTCGGTATGC AGATATTGTA ATATCGGCTA GAGATCGAAT TTTAGAACAA CAAGAAATAC AAATTGGTAT TTCAAGTAGA TCTGTAAAAA TGCCCAGTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT