BaroA.17330.a

Bep2 protein (VirB-translocated Bartonella effector protein)

CENTER ID: BaroA.17330.a
ORGANISM: Bartonella rochalimae ATCC BAA-1498
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BaroA.17330.a.B2.GE36588 FIC 20 246
BaroA.17330.a.B3.GE36589 FIC and truncated OB-Fold 20 302
BaroA.17330.a.B4.GE37266 BID 313 441
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|685782.3.peg.494
RefSeq: WP_035006139.1
UniProt: E6YMI0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKSEMMIKL EDINIPSPKN YVYPDSRVLK NKYGIMELQK FEERMTHDAL KETVKVLEEP APERFDSSYL KYLHKRLFQH ALEWAGCTRD VPFTFLDGTR AVMPSMAKCD SDFGFAFGSE IKEGLDFIDQ KLSEKNYLRG LSREEFVHNA AKIFGSINYI HPFREGNGRV QRLFFVKLAR SVGYKLDFSA TTTKRMTYVS VEVTKNAYMK PLQHLFEDIS NPEKVGILKQ FISNAKEYNI FDSNEKLITV AREGETYKGI GKFLASDFIV IEAGDTCILS NKDSLTPEQL RTFKLGSDFI FTALDTKDLK EIFIPEEKLA ALSEDEMIEK ILEYSSVREQ RDKIQHCARF IYGHSDQLNE IINMIDMDPN LGEVFSEQIM ACCDNKKYFI SKKTKATNQS FCKLSNEIKN YANIVEDSRN QILKEYKERQ KRCGQVVKMP SKEVQCILDM PEDMWEKALK SWSLSFLNKS ISDFIIKVNH RLSPCECKAI NDKNYEELAK SISISESKAK AIIKTIGKVK EIQKKIQVLR ANALKATAMV C
NT Sequence
atgaagaaaa gtgaaatgat gataaagttg gaggatatta acatcccttc tcctaaaaat tatgtctatc ctgacagtag agtgttgaag aataaatatg gaatcatgga gcttcaaaag ttcgaggaga gaatgacgca tgatgcatta aaagaaactg ttaaggtgct agaagaacct gctccagagc ggtttgattc ttcttatcta aagtatttgc ataaacgttt atttcagcat gcattggaat gggctgggtg cacccgtgat gttccattta catttttaga tggtacaagg gctgtgatgc cctccatggc aaagtgtgat tccgattttg gttttgcatt tggttcagag attaaggaag gtttagattt catcgatcaa aaactctctg agaagaatta tttgagaggc ttatcgcgtg aagaatttgt tcataatgca gcaaaaatat ttggctctat caattatatt catcctttta gggaaggtaa tggacgcgta caaagattgt tttttgtaaa gctagccaga tctgtaggct ataaacttga cttttccgct acaacaacaa agcgcatgac atatgtgagt gttgaggtta caaaaaacgc atatatgaag cctttgcaac atttgtttga agatatttcc aatccagaaa aggtcggtat tttaaagcaa ttcataagca atgccaaaga atataatatc tttgattcga atgaaaagtt gattacagta gcgagagaag gtgaaaccta taaggggatt ggtaaattcc ttgcttcaga ttttattgta atagaagcgg gagatacttg tatcctctcc aataaagatt cccttacacc agaacaatta aggacattca aacttggtag tgatttcatt tttacagctt tagatactaa agatcttaaa gaaattttta tcccagaaga aaaattagct gctttgagtg aagatgaaat gattgaaaaa attttagaat atagttctgt tcgagagcaa cgagataaaa tccagcattg tgcaagattt atttatggac attcggatca attgaacgag ataataaaca tgattgatat ggatccgaat ttaggggagg tgttttctga acaaattatg gcatgttgtg ataacaaaaa gtatttcata agtaaaaaaa ctaaagctac taatcaaagt ttttgtaaac tgagtaatga aattaaaaat tatgcaaata ttgtagaaga ttctagaaat caaatcttaa aggagtataa agaaagacaa aaacgttgtg gacaggtagt aaaaatgccc agcaaggaag tgcaatgcat tcttgatatg ccagaagata tgtgggaaaa agctctgaaa tcatggagtc tttcatttct taataaaagt atctctgatt ttatcattaa agttaatcac cgtctttcac catgtgaatg taaggcaatc aatgataaga actatgaaga acttgctaaa agtattagca tatcagaaag taaagcaaag gcaattataa aaactattgg gaaagtcaaa gaaatacaaa agaaaataca agtacttaga gcgaatgctt taaaggcgac agctatggtt tgttaa
Details for BaroA.17330.a.B2.GE36588
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 28kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN YVYPDSRVLK NKYGIMELQK FEERMTHDAL KETVKVLEEP APERFDSSYL KYLHKRLFQH ALEWAGCTRD VPFTFLDGTR AVMPSMAKCD SDFGFAFGSE IKEGLDFIDQ KLSEKNYLRG LSREEFVHNA AKIFGSINYI HPFREGNGRV QRLFFVKLAR SVGYKLDFSA TTTKRMTYVS VEVTKNAYMK PLQHLFEDIS NPEKVGILKQ FISNAKEYNI FDSNEK
Validated NT Sequence
ttntnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgaattat gtctatcctg acagtagagt gttgaagaat aaatatggaa tcatggagct tcaaaagttc gaggagagaa tgacgcatga tgcattaaaa gaaactgtta aggtgctaga agaacctgct ccagagcggt ttgattcttc ttatctaaag tatttgcata aacgtttatt tcagcatgca ttggaatggg ctgggtgcac ccgtgatgtt ccatttacat ttttagatgg tacaagggct gtgatgccct ccatggcaaa gtgtgattcc gattttggtt ttgcatttgg ttcagagatt aaggaaggtt tagatttcat cgatcaaaaa ctctctgaga agaattattt gagaggctta tcgcgtgaag aatttgttca taatgcagca aaaatatttg gctctatcaa ttatattcat ccttttaggg aaggtaatgg acgcgtacaa agattgtttt ttgtaaagct agccagatct gtaggctata aacttgactt ttccgctaca acaacaaagc gcatgacata tgtgagtgtt gaggttacaa aaaacgcata tatgaagcct ttgcaacatt tgtttgaaga tatttccaat ccagaaaagg tcggtatttt aaagcaattc ataagcaatg ccaaagaata taatatcttt gattcgaatg aaaagtaagt gagtaagata ggatccggnn nnnnnnnccc nnnnnannnn nnnnnnnaaa annnnntnnn ntgnantnnn ancnggagan nnntnccnct cnatnnnnat tcncttnnnn nnnnnnnntn nnnnnttcan ncntnnngtg anncatttnn catannganc
Expected Protein Sequence
MAHHHHHHMN YVYPDSRVLK NKYGIMELQK FEERMTHDAL KETVKVLEEP APERFDSSYL KYLHKRLFQH ALEWAGCTRD VPFTFLDGTR AVMPSMAKCD SDFGFAFGSE IKEGLDFIDQ KLSEKNYLRG LSREEFVHNA AKIFGSINYI HPFREGNGRV QRLFFVKLAR SVGYKLDFSA TTTKRMTYVS VEVTKNAYMK PLQHLFEDIS NPEKVGILKQ FISNAKEYNI FDSNEK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt atgtctatcc tgacagtaga gtgttgaaga ataaatatgg aatcatggag cttcaaaagt tcgaggagag aatgacgcat gatgcattaa aagaaactgt taaggtgcta gaagaacctg ctccagagcg gtttgattct tcttatctaa agtatttgca taaacgttta tttcagcatg cattggaatg ggctgggtgc acccgtgatg ttccatttac atttttagat ggtacaaggg ctgtgatgcc ctccatggca aagtgtgatt ccgattttgg ttttgcattt ggttcagaga ttaaggaagg tttagatttc atcgatcaaa aactctctga gaagaattat ttgagaggct tatcgcgtga agaatttgtt cataatgcag caaaaatatt tggctctatc aattatattc atccttttag ggaaggtaat ggacgcgtac aaagattgtt ttttgtaaag ctagccagat ctgtaggcta taaacttgac ttttccgcta caacaacaaa gcgcatgaca tatgtgagtg ttgaggttac aaaaaacgca tatatgaagc ctttgcaaca tttgtttgaa gatatttcca atccagaaaa ggtcggtatt ttaaagcaat tcataagcaa tgccaaagaa tataatatct ttgattcgaa tgaaaagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BaroA.17330.a.B3.GE36589
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 105
Validated AA Sequence
MAHHHHHHMN YVYPDSRVLK NKYGIMELQK FEERMTHDAL KETVKVLEEP APERFDSSYL KYLHKRLFQH ALEWAGCTRD VPFTFLDGTR AVMPSMAKCD SDFGFAFGSE IKEGLDFIDQ KLSEKNYLRG LSREEFVHNA AKIFGSINYI HPFREGNGRV QRLFFVKLAR SVGYKLDFSA TTTKRMTYVS VEVTKNAYMK PLQHLFEDIS NPEKVGILKQ FISNAKEYNI FDSNEKLITV AREGETYKGI GKFLASXFIV IEAGDTCILS XKXSLTPEQL RXFKLGSDFX FTXVXXXXXX KXXXXKKKK
Validated NT Sequence
cnnnnnnnnn nttnttnanc tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt atgtctatcc tgacagtaga gtgttgaaga ataaatatgg aatcatggag cttcaaaagt tcgaggagag aatgacgcat gatgcattaa aagaaactgt taaggtgcta gaagaacctg ctccagagcg gtttgattct tcttatctaa agtatttgca taaacgttta tttcagcatg cattggaatg ggctgggtgc acccgtgatg ttccatttac atttttagat ggtacaaggg ctgtgatgcc ctccatggca aagtgtgatt ccgattttgg ttttgcattt ggttcagaga ttaaggaagg tttagatttc atcgatcaaa aactctctga gaagaattat ttgagaggct tatcgcgtga agaatttgtt cataatgcag caaaaatatt tggctctatc aattatattc atccttttag ggaaggtaat ggacgcgtac aaagattgtt ttttgtaaag ctagccagat ctgtaggcta taaacttgac ttttccgcta caacaacaaa gcgcatgaca tatgtgagtg ttgaggttac aaaaaacgca tatatgaagc ctttgcaaca tttgtttgaa gatatttcca atccagaaaa ggtcggtatt ttaaagcaat tcataagcaa tgccaaagaa tataatatct ttgattcgaa tgaaaagttg attacagtag cgagagaagg tgaaacctat aaggggattg gtaaattcct tgcttcanat tttattgtaa tagaagcggg agatacttgt atcctctcnn ataaanattc ccttacacca gaacaattaa ggncattcaa acttggtagt gatttcnttt ttacntnagt gannnnannn nannnnnnna aaaannnnnn nnnaaaaaaa aaaaaaaa
Expected Protein Sequence
MAHHHHHHMN YVYPDSRVLK NKYGIMELQK FEERMTHDAL KETVKVLEEP APERFDSSYL KYLHKRLFQH ALEWAGCTRD VPFTFLDGTR AVMPSMAKCD SDFGFAFGSE IKEGLDFIDQ KLSEKNYLRG LSREEFVHNA AKIFGSINYI HPFREGNGRV QRLFFVKLAR SVGYKLDFSA TTTKRMTYVS VEVTKNAYMK PLQHLFEDIS NPEKVGILKQ FISNAKEYNI FDSNEKLITV AREGETYKGI GKFLASDFIV IEAGDTCILS NKDSLTPEQL RTFKLGSDFI FT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt atgtctatcc tgacagtaga gtgttgaaga ataaatatgg aatcatggag cttcaaaagt tcgaggagag aatgacgcat gatgcattaa aagaaactgt taaggtgcta gaagaacctg ctccagagcg gtttgattct tcttatctaa agtatttgca taaacgttta tttcagcatg cattggaatg ggctgggtgc acccgtgatg ttccatttac atttttagat ggtacaaggg ctgtgatgcc ctccatggca aagtgtgatt ccgattttgg ttttgcattt ggttcagaga ttaaggaagg tttagatttc atcgatcaaa aactctctga gaagaattat ttgagaggct tatcgcgtga agaatttgtt cataatgcag caaaaatatt tggctctatc aattatattc atccttttag ggaaggtaat ggacgcgtac aaagattgtt ttttgtaaag ctagccagat ctgtaggcta taaacttgac ttttccgcta caacaacaaa gcgcatgaca tatgtgagtg ttgaggttac aaaaaacgca tatatgaagc ctttgcaaca tttgtttgaa gatatttcca atccagaaaa ggtcggtatt ttaaagcaat tcataagcaa tgccaaagaa tataatatct ttgattcgaa tgaaaagttg attacagtag cgagagaagg tgaaacctat aaggggattg gtaaattcct tgcttcagat tttattgtaa tagaagcggg agatacttgt atcctctcca ataaagattc ccttacacca gaacaattaa ggacattcaa acttggtagt gatttcattt ttacatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BaroA.17330.a.B4.GE37266
HARVESTED ON: 8/28/2013
SEQUENCED ON: 9/3/2013
EXPECTED MW: 16kDa
OBSERVED MW: 15kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMF IPEEKLAALS EDEMIEKILE YSSVREQRDK IQHCARFIYG HSDQLNEIIN MIDMDPNLGE VFSEQIMACC DNKKYFISKK TKATNQSFCK LSNEIKNYAN IVEDSRNQIL KEYKERQKRC GQVVKMPS
Validated NT Sequence
atcttactca cttagctggg catttttact acctgtccac aacgtttttg tctttcttta tactccttta agatttgatt tctagaatct tctacaatat ttgcataatt tttaatttca ttactcagtt tacaaaaact ttgattagta gctttagttt ttttacttat gaaatacttt ttgttatcac aacatgccat aatttgttca gaaaacacct cccctaaatt cggatccata tcaatcatgt ttattatctc gttcaattga tccgaatgtc cataaataaa tcttgcacaa tgctggattt tatctcgttg ctctcgaaca gaactatatt ctaaaatttt ttcaatcatt tcatcttcac tcaaagcagc taatttttct tctgggataa acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHFI PEEKLAALSE DEMIEKILEY SSVREQRDKI QHCARFIYGH SDQLNEIINM IDMDPNLGEV FSEQIMACCD NKKYFISKKT KATNQSFCKL SNEIKNYANI VEDSRNQILK EYKERQKRCG QVVKMPS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattttatcc cagaagaaaa attagctgct ttgagtgaag atgaaatgat tgaaaaaatt ttagaatata gttctgttcg agagcaacga gataaaatcc agcattgtgc aagatttatt tatggacatt cggatcaatt gaacgagata ataaacatga ttgatatgga tccgaattta ggggaggtgt tttctgaaca aattatggca tgttgtgata acaaaaagta tttcataagt aaaaaaacta aagctactaa tcaaagtttt tgtaaactga gtaatgaaat taaaaattat gcaaatattg tagaagattc tagaaatcaa atcttaaagg agtataaaga aagacaaaaa cgttgtggac aggtagtaaa aatgcccagc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat