SSGCID: Target Page

BaroA.01664.a

Two-component sensor kinase BatS (EC 2.7.3.-) (BatR-BatS two-component system)

CENTER ID:	BaroA.01664.a
ORGANISM:	Bartonella rochalimae ATCC BAA-1498
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaroA.01664.a.B2.GE36078	kinase domain		294	555
BaroA.01664.a.B3.GE37265	periplasmic domain		29	240

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	E6YJT3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFFSSLTRRI VILNLAALGI LVISILYLNQ FRNGLIEAKI ESLRTQGKII AGAIAASATI DTNSILIDPQ KLLELQAGES VTPMLQSTDT WDFPINSEQV APLLRRLITP KTTRARIYDR DATLLLDSRV LYSSGEVFTY DLPPIQTKQN LWEHLKSLFF NTLYGNGIAV DRRQAKDNEA LHPEVYLALN GSSATAKRRN RQGQLIVSVA VPVQRYRAVV GALLLSTIGT DIDDIVESER LGIFKIFAVV GSVFLVLSLF LAHTIAHPLS KLSSSANRVR HGRSKRITIP DFSKREDEIG RLSTSIRDMT NALYTRIEAI ERFAADVSHE LKNPLTSLRS AVETLPLAKN KAQQTQLLEI IQHDVHRLDR LITDISDASR LDAELVRETA KIVDMGSLLK SLIQAIREIH CNKQNIDINL NIIPRINGKP YLIVGHELRL GQVISNLIEN ARSFVPRKNG KICITMKNNA SNLILTVEDN GPGICSENIE RIFERFYTDR SGKDAFGKHS GLGLAISQQI IEAHNGTLMA ENIIDPKSKK EKIGARFIIT LPLTK

NT Sequence

ttgttctttt ccagtctcac acgccgtatt gtgattttaa atcttgctgc ccttggtatt ctagttataa gtattttgta tcttaaccaa tttcgtaatg gtttgattga agcaaaaatt gaaagtttac gcacccaagg aaaaattatt gctggagcta ttgctgcttc tgcaacaata gacacaaact ctattttaat tgaccctcaa aaactgttgg aattacaagc tggagaaagt gttacaccaa tgcttcaatc cactgatact tgggattttc ccattaattc cgaacaagtt gctccacttt tacgacgtct tattacacca aaaacaacaa gagcacggat atatgaccgt gacgcaactt tgcttcttga ttcacgtgtt ctttattcga gtggtgaggt ttttacttat gatttacctc ctatccaaac aaaacaaaat ttatgggagc acttaaaatc attgtttttt aatacactct atggcaacgg tattgctgtt gatagaagac aagcaaaaga taatgaagct cttcatcctg aagtttatct agcattaaat ggctcttctg ccactgctaa acgacgtaac aggcaaggtc aattaattgt ttcagttgct gtacctgttc aacgttatcg tgcagtcgtt ggtgctcttt tgctttcaac tattggtaca gacattgatg atattgtgga aagtgaaaga ttaggaattt ttaaaatctt tgccgttgtt ggaagtgttt tcttggttct ttctctattt ttagctcata ctattgcgca tccactaagt aaattatctt cctccgctaa tcgcgtgcgt catggtcgca gtaagagaat aacaattcct gatttttcaa aacgtgaaga tgaaattggt cgtctttcta cctctattcg tgatatgacc aacgctcttt atacacgcat tgaagccatt gaacgttttg cagccgatgt aagtcatgaa ctaaagaatc ctcttacctc gctgcgcagt gccgttgaaa cattaccact ggctaaaaat aaagcacaac agacacaatt acttgaaatc atccaacatg acgtgcatcg tcttgatcgt ttaattaccg atatttctga cgcatcgcga ttagatgcag aacttgttcg cgaaactgct aaaatagttg atatgggttc attgttaaaa agccttattc aagctattcg tgaaatccat tgtaataaac aaaatattga tattaatttg aatattattc cacgaattaa tggcaaacct tacctaattg tagggcatga attacgttta gggcaggtga tttccaatct cattgaaaat gcacgttctt ttgtacctcg taaaaatggt aaaatttgta ttacaatgaa aaataatgct tcaaatctca ttttaactgt cgaagacaat ggaccaggta tttgttcaga aaatattgaa cgtatttttg aacgtttcta tactgatcgg tcaggcaaag atgcttttgg gaaacattca ggtcttggcc ttgcaattag tcagcaaatc attgaagctc ataatggaac tctcatggct gaaaatatta ttgatcccaa atctaaaaaa gaaaaaattg gtgctcgatt tattatcacc cttccactta ccaaataa

Details for BaroA.01664.a.B2.GE36078

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK REDEIGRLST SIRDMTNALY TRIEAIERFA ADVSHELKNP LTSLRSAVET LPLAKNKAQQ TQLLEIIQHD VHRLDRLITD ISDASRLDAE LVRETAKIVD MGSLLKSLIQ AIREIHCNKQ NIDINLNIIP RINGKPYLIV GHELRLGQVI SNLIENARSF VPRKNGKICI TMKNNASNLI LTVEDNGPGI CSENIERIFE RFYTDRSGKD AFGKHSGLGL AISQQIIEAH NGTLMAENII DPKSKKEKIG ARFIITLPLT K

Validated NT Sequence

atcttactca cttatttggt aagtggaagg gtgataataa atcgagcacc aattttttct tttttagatt tgggatcaat aatattttca gccatgagag ttccattatg agcttcaatg atttgctgac taattgcaag gccaagacct gaatgtttcc caaaagcatc tttgcctgac cgatcagtat agaaacgttc aaaaatacgt tcaatatttt ctgaacaaat acctggtcca ttgtcttcga cagttaaaat gagatttgaa gcattatttt tcattgtaat acaaatttta ccatttttac gaggtacaaa agaacgtgca ttttcaatga gattggaaat cacctgccct aaacgtaatt catgccctac aattaggtaa ggtttgccat taattcgtgg aataatattc aaattaatat caatattttg tttattacaa tggatttcac gaatagcttg aataaggctt tttaacaatg aacccatatc aactatttta gcagtttcgc gaacaagttc tgcatctaat cgcgatgcgt cagaaatatc ggtaattaaa cgatcaagac gatgcacgtc atgttggatg atttcaagta attgtgtctg ttgtgcttta tttttagcca gtggtaatgt ttcaacggca ctgcgcagcg aggtaagagg attctttagt tcatgactta catcggctgc aaaacgttca atggcttcaa tgcgtgtata aagagcgttg gtcatatcac gaatagaggt agaaagacga ccaatttcat cttcacgttt catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac gtgaagatga aattggtcgt ctttctacct ctattcgtga tatgaccaac gctctttata cacgcattga agccattgaa cgttttgcag ccgatgtaag tcatgaacta aagaatcctc ttacctcgct gcgcagtgcc gttgaaacat taccactggc taaaaataaa gcacaacaga cacaattact tgaaatcatc caacatgacg tgcatcgtct tgatcgttta attaccgata tttctgacgc atcgcgatta gatgcagaac ttgttcgcga aactgctaaa atagttgata tgggttcatt gttaaaaagc cttattcaag ctattcgtga aatccattgt aataaacaaa atattgatat taatttgaat attattccac gaattaatgg caaaccttac ctaattgtag ggcatgaatt acgtttaggg caggtgattt ccaatctcat tgaaaatgca cgttcttttg tacctcgtaa aaatggtaaa atttgtatta caatgaaaaa taatgcttca aatctcattt taactgtcga agacaatgga ccaggtattt gttcagaaaa tattgaacgt atttttgaac gtttctatac tgatcggtca ggcaaagatg cttttgggaa acattcaggt cttggccttg caattagtca gcaaatcatt gaagctcata atggaactct catggctgaa aatattattg atcccaaatc taaaaaagaa aaaattggtg ctcgatttat tatcaccctt ccacttacca aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for BaroA.01664.a.B3.GE37265

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN QFRNGLIEAK IESLRTQGKI IAGAIAASAT IDTNSILIDP QKLLELQAGE SVTPMLQSTD TWDFPINSEQ VAPLLRRLIT PKTTRARIYD RDATLLLDSR VLYSSGEVFT YDLPPIQTKQ NLWEHLKSLF FNTLYGNGIA VDRRQAKDNE ALHPEVYLAL NGSSATAKRR NRQGQLIVSV AVPVQRYRAV VGALLLSTIG TDIDDIVESE R

Validated NT Sequence

tnnnnnanat tttnttnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgaacca atttcgtaat ggtttgattg aagcaaaaat tgaaagttta cgcacccaag gaaaaattat tgctggagct attgctgctt ctgcaacaat agacacaaac tctattttaa ttgaccctca aaaactgttg gaattacaag ctggagaaag tgttacacca atgcttcaat ccactgatac ttgggatttt cccattaatt ccgaacaagt tgctccactt ttacgacgtc ttattacacc aaaaacaaca agagcacgga tatatgaccg tgacgcaact ttgcttcttg attcacgtgt tctttattcg agtggtgagg tttttactta tgatttacct cctatccaaa caaaacaaaa tttatgggag cacttaaaat cattgttttt taatacactc tatggcaacg gtattgctgt tgatagaaga caagcaaaag ataatgaagc tcttcatcct gaagtttatc tagcattaaa tggctcttct gccactgcta aacgacgtaa caggcaaggt caattaattg tttcagttgc tgtacctgtt caacgttatc gtgcagtcgt tggtgctctt ttgctttcaa ctattggtac agacattgat gatattgtgg aaagtgaaag ataagtgagt aaganaggat ccggntnnnn nnnnccccnn nnnnnnnnna angnnnnnnn aaaaannnnn ncccgganaa nttnnnnnnn nnnantncna annnnnnnna ttnccnncnn nnncaacnnn nacagannag annananann nnnnnnnna

Expected Protein Sequence

MAHHHHHHNQ FRNGLIEAKI ESLRTQGKII AGAIAASATI DTNSILIDPQ KLLELQAGES VTPMLQSTDT WDFPINSEQV APLLRRLITP KTTRARIYDR DATLLLDSRV LYSSGEVFTY DLPPIQTKQN LWEHLKSLFF NTLYGNGIAV DRRQAKDNEA LHPEVYLALN GSSATAKRRN RQGQLIVSVA VPVQRYRAVV GALLLSTIGT DIDDIVESER

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaccaat ttcgtaatgg tttgattgaa gcaaaaattg aaagtttacg cacccaagga aaaattattg ctggagctat tgctgcttct gcaacaatag acacaaactc tattttaatt gaccctcaaa aactgttgga attacaagct ggagaaagtg ttacaccaat gcttcaatcc actgatactt gggattttcc cattaattcc gaacaagttg ctccactttt acgacgtctt attacaccaa aaacaacaag agcacggata tatgaccgtg acgcaacttt gcttcttgat tcacgtgttc tttattcgag tggtgaggtt tttacttatg atttacctcc tatccaaaca aaacaaaatt tatgggagca cttaaaatca ttgtttttta atacactcta tggcaacggt attgctgttg atagaagaca agcaaaagat aatgaagctc ttcatcctga agtttatcta gcattaaatg gctcttctgc cactgctaaa cgacgtaaca ggcaaggtca attaattgtt tcagttgctg tacctgttca acgttatcgt gcagtcgttg gtgctctttt gctttcaact attggtacag acattgatga tattgtggaa agtgaaagat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat