SSGCID: Target Page

BaquA.18412.a

Type IV secretion system protein virB4 (VirB type IV secretion machinery)

CENTER ID:	BaquA.18412.a
ORGANISM:	Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaquA.18412.a.B1.GE36076	full length		1	784

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283165.4.peg.1143
RefSeq:	YP_032622.1
UniProt:	Q6FYW7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSIMKRESLP EEYIPYIRHV NQHVIALNSR CLMTVMAVEG VNFDTADINH LNSLHNQLNT LLRNIADERV ALYSHIIRRR ETIYPESRFF SSFAATLDEK YKKKMVSQEL YRNDLFVSLL WNPTSGKTEQ LASFFQRLTK AKKTQSEPDM EAIRKIEELS QDLIQGLESY EARLLSVYAH EGILFSEQSE FLHQLVGGRR ERIPLTFGTI ASTIYSDRVI FGKEMIEIRH ESNERFVGMF GWKEYPSKTR PGMTDGLLTA PFEFILTQSF VFKSKAAARV IMGRKQNQMI NAADRASSQI DALDEALDDL ESNRFVLGEH HLSLAVFADQ PKTLVEYLSK ARAHLTNGGA VIAREDLGLE AAWWAQLPGN FSYRARSGAI TSRNFAALSP FHSFPIGKLE GNVWGAAVAL LKTQAGSPYY FNFHYGDLGN TFVCGPSGSG KTVIVNFLLA QLQKHNPTMV FFDKDQGAEI FVRAGGGKYK PLKNGQPTGI APLKGMEYTE KNKIFLRSWV LKLVTTEGQT VTEQERQDIA KAINSLESLP HAQRSLGALQ LFFDNTSKEG IAIRLQRWIK GNDLGWVFDN DQDDLNLDSQ FIGYDMTDFL DNEEIRRPLM MYLFNRILDL IDGRRIIIVI DEFWKALEDD SFKAFAQDRL KTIRKQNGMM LFATQSPKDA LNSTIAHTII EQCPTQIFFP NQKANYKDYV EDFKLTEREF ELIQSELSRE SRRFLIKQGQ NSVVAELNLR GMNDEIAILS GTTKNIELVN QIINDYGADP DTWLPIFHQR RENQ

NT Sequence

atgtcaatta tgaaacggga gtctttacct gaagaatata ttccttacat acgccacgtc aaccaacacg tcattgcatt aaattcacgc tgcttaatga ctgtaatggc tgttgagggg gtgaattttg atactgcaga tatcaatcac ttaaattcct tacacaacca gttaaacact ctcttgagaa atatcgcgga tgaacgtgtt gctttatatt ctcacatcat tcgtcggcgc gaaacgatct atccagagag tcgttttttt tcatcttttg cagcaacact agatgaaaaa tacaaaaaga aaatggtttc gcaagagctg tatagaaatg atctcttcgt ttcactgctg tggaatccaa catcgggtaa gactgagcaa ctcgcttcat tttttcagcg cttaacaaaa gcgaagaaaa cacaatctga accagacatg gaagccattc gtaaaattga agagttaagc caagatctta tacaagggtt ggagagctat gaagcgcgcc tcttgtcagt ctatgcacat gagggcattt tgttttccga acaaagtgaa tttctacacc agttagtggg aggaaggcgt gagcggattc ctctcacatt tggtaccatt gcctcaacga tttactcaga ccgtgtcatt tttggcaaag aaatgatcga aattcgtcat gaaagcaatg aacgctttgt tggcatgttt ggctggaaag aatatccctc taaaacacgc ccaggtatga cagatggttt actcacagca ccgtttgaat ttatcttaac acaatccttt gtctttaaga gtaaagcagc tgccagggtc attatgggcc gcaaacaaaa tcagatgatt aatgcagcgg atcgtgctag ctcgcaaatt gatgcacttg atgaagcgct tgatgattta gaatcaaacc gttttgtttt gggcgaacat catctctctc tagccgtttt tgctgatcaa ccaaaaacat tggttgaata cctctcaaaa gcgcgcgctc acttaaccaa tggtggagcg gttatcgcca gagaagatct aggattagaa gctgcatggt gggcgcaact gcctggaaat ttcagctatc gtgcgcgatc tggagccatt accagcagaa attttgcagc gttatcgccc ttccattcct tccccattgg caaacttgaa ggcaatgttt ggggagcggc tgtggcattg ctgaaaacac aagctggttc accttattat tttaattttc attatggtga ccttggcaac acttttgttt gcggtccatc aggatctggt aaaactgtga ttgttaattt ccttctcgca caattacaaa aacataaccc gacaatggtc ttttttgaca aagatcaagg cgcagagatt tttgtgcgtg ctggaggtgg aaaatataag cctttgaaaa acggacagcc cacgggcatt gctccattaa agggcatgga atacactgaa aaaaacaaaa tctttcttcg cagttgggtc ttgaagctgg tgacaactga agggcaaaca gtgacagaac aagagcgaca agatatcgcc aaagccataa attccttgga aagtcttcca catgcgcaac gctctcttgg tgcccttcaa ttgttttttg ataacacatc aaaagaagga attgctatac ggttacaacg ctggatcaaa ggcaatgact taggctgggt ttttgacaac gatcaagatg atctcaattt agactcacaa ttcattggtt atgacatgac cgatttctta gacaatgaag aaattcggcg ccccttgatg atgtatctgt ttaaccgcat tctcgatctt attgatgggc ggcgcattat tattgtcatt gatgaattct ggaaagcgct tgaagatgat tcctttaaag cttttgcgca agatcgcctt aagacgatcc gtaaacaaaa tggtatgatg ctctttgcta cgcaaagccc caaagatgct ttgaactcta caatcgcaca cacgattatt gagcaatgcc ctacccaaat attttttcca aatcaaaaag caaattacaa agattatgtt gaagatttta aactgactga gcgtgaattt gaactgatac agtcagaatt aagcagagaa tctcgtcgtt ttctcataaa acagggacaa aattcggtcg ttgcagaact caatttacgt ggaatgaacg atgagatcgc catcttaagc ggtacgacca aaaacatcga actggtgaac caaattatca acgattatgg agcagatccc gatacatggc tgcctatatt tcaccaaagg agagaaaatc aatga

Details for BaquA.18412.a.B1.GE36076

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	90kDa
OBSERVED MW:	80kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	37

Validated AA Sequence

Validated NT Sequence

attttgttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgtca attatgaaac gggagtcttt acctgaagaa tatattcctt acatacgcca cgtcaaccaa cacgtcattg cattaaattc acgctgctta atgactgtaa tggctgttga gggggtgaat tttgatactg cagatatcaa tcacttaaat tccttacaca accagttaaa cactctcttg agaaatatcg cggatgaacg tgttgcttta tattctcaca tcattcgtcg gcgcgaaacg atctatccag agagtcgttt tttttcatct tttgcagcaa cactagatga aaaatacaaa aagaaaatgg tttcgcaaga gctgtataga aatgatctct tcgtttcact gctgtggaat ccaacatcgg gtaagactga gcaactcgct tcattttttc agcgcttaac aaaagcgaag aaaacacaat ctgaaccaga catggaagcc attcgtaaaa ttgaagagtt aagccaagat cttatacaag ggttggagag ctatgaagcg cgcctcttgt cagtctatgc acatgagggc attttgtttt ccgaacaaag tgaatttcta caccagttag tgggaggaag gcgtgagcgg attcctctca catttggtac cattgcctca acgatttact cagaccgtgt catttttggc aaagaaatga tcgaaattcg tcatgaaagc aatgaacgct ttgttggcat gtttggctgg aaagaatatc cctctaaaac acgcccaggt atgacagatg gtttactcac agcaccgttt gaatttatct taacacaatc ctttgtcttt aanagtaaag cagctgccng gncattatgg gccgcancaa aatcagatga ttaatgcagc ggatcgtgct agctcgcnan tgatgcncnn gatgaagcnn nnnngatnnn ancaancnnt tgnttgggcn anntcatctn ncncnancnt tttnctgatn aannnnnnna nacnnnnnaa gnnnnngcnn nnntnnnnnn nnnannnnan nnnngnnnna ancnncnngn nngnnnnnnn ncnnnnnnnn nnnnnnnnnn nnnnnnnnnn cnnct

Expected Protein Sequence

MAHHHHHHMS IMKRESLPEE YIPYIRHVNQ HVIALNSRCL MTVMAVEGVN FDTADINHLN SLHNQLNTLL RNIADERVAL YSHIIRRRET IYPESRFFSS FAATLDEKYK KKMVSQELYR NDLFVSLLWN PTSGKTEQLA SFFQRLTKAK KTQSEPDMEA IRKIEELSQD LIQGLESYEA RLLSVYAHEG ILFSEQSEFL HQLVGGRRER IPLTFGTIAS TIYSDRVIFG KEMIEIRHES NERFVGMFGW KEYPSKTRPG MTDGLLTAPF EFILTQSFVF KSKAAARVIM GRKQNQMINA ADRASSQIDA LDEALDDLES NRFVLGEHHL SLAVFADQPK TLVEYLSKAR AHLTNGGAVI AREDLGLEAA WWAQLPGNFS YRARSGAITS RNFAALSPFH SFPIGKLEGN VWGAAVALLK TQAGSPYYFN FHYGDLGNTF VCGPSGSGKT VIVNFLLAQL QKHNPTMVFF DKDQGAEIFV RAGGGKYKPL KNGQPTGIAP LKGMEYTEKN KIFLRSWVLK LVTTEGQTVT EQERQDIAKA INSLESLPHA QRSLGALQLF FDNTSKEGIA IRLQRWIKGN DLGWVFDNDQ DDLNLDSQFI GYDMTDFLDN EEIRRPLMMY LFNRILDLID GRRIIIVIDE FWKALEDDSF KAFAQDRLKT IRKQNGMMLF ATQSPKDALN STIAHTIIEQ CPTQIFFPNQ KANYKDYVED FKLTEREFEL IQSELSRESR RFLIKQGQNS VVAELNLRGM NDEIAILSGT TKNIELVNQI INDYGADPDT WLPIFHQRRE NQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa ttatgaaacg ggagtcttta cctgaagaat atattcctta catacgccac gtcaaccaac acgtcattgc attaaattca cgctgcttaa tgactgtaat ggctgttgag ggggtgaatt ttgatactgc agatatcaat cacttaaatt ccttacacaa ccagttaaac actctcttga gaaatatcgc ggatgaacgt gttgctttat attctcacat cattcgtcgg cgcgaaacga tctatccaga gagtcgtttt ttttcatctt ttgcagcaac actagatgaa aaatacaaaa agaaaatggt ttcgcaagag ctgtatagaa atgatctctt cgtttcactg ctgtggaatc caacatcggg taagactgag caactcgctt cattttttca gcgcttaaca aaagcgaaga aaacacaatc tgaaccagac atggaagcca ttcgtaaaat tgaagagtta agccaagatc ttatacaagg gttggagagc tatgaagcgc gcctcttgtc agtctatgca catgagggca ttttgttttc cgaacaaagt gaatttctac accagttagt gggaggaagg cgtgagcgga ttcctctcac atttggtacc attgcctcaa cgatttactc agaccgtgtc atttttggca aagaaatgat cgaaattcgt catgaaagca atgaacgctt tgttggcatg tttggctgga aagaatatcc ctctaaaaca cgcccaggta tgacagatgg tttactcaca gcaccgtttg aatttatctt aacacaatcc tttgtcttta agagtaaagc agctgccagg gtcattatgg gccgcaaaca aaatcagatg attaatgcag cggatcgtgc tagctcgcaa attgatgcac ttgatgaagc gcttgatgat ttagaatcaa accgttttgt tttgggcgaa catcatctct ctctagccgt ttttgctgat caaccaaaaa cattggttga atacctctca aaagcgcgcg ctcacttaac caatggtgga gcggttatcg ccagagaaga tctaggatta gaagctgcat ggtgggcgca actgcctgga aatttcagct atcgtgcgcg atctggagcc attaccagca gaaattttgc agcgttatcg cccttccatt ccttccccat tggcaaactt gaaggcaatg tttggggagc ggctgtggca ttgctgaaaa cacaagctgg ttcaccttat tattttaatt ttcattatgg tgaccttggc aacacttttg tttgcggtcc atcaggatct ggtaaaactg tgattgttaa tttccttctc gcacaattac aaaaacataa cccgacaatg gtcttttttg acaaagatca aggcgcagag atttttgtgc gtgctggagg tggaaaatat aagcctttga aaaacggaca gcccacgggc attgctccat taaagggcat ggaatacact gaaaaaaaca aaatctttct tcgcagttgg gtcttgaagc tggtgacaac tgaagggcaa acagtgacag aacaagagcg acaagatatc gccaaagcca taaattcctt ggaaagtctt ccacatgcgc aacgctctct tggtgccctt caattgtttt ttgataacac atcaaaagaa ggaattgcta tacggttaca acgctggatc aaaggcaatg acttaggctg ggtttttgac aacgatcaag atgatctcaa tttagactca caattcattg gttatgacat gaccgatttc ttagacaatg aagaaattcg gcgccccttg atgatgtatc tgtttaaccg cattctcgat cttattgatg ggcggcgcat tattattgtc attgatgaat tctggaaagc gcttgaagat gattccttta aagcttttgc gcaagatcgc cttaagacga tccgtaaaca aaatggtatg atgctctttg ctacgcaaag ccccaaagat gctttgaact ctacaatcgc acacacgatt attgagcaat gccctaccca aatatttttt ccaaatcaaa aagcaaatta caaagattat gttgaagatt ttaaactgac tgagcgtgaa tttgaactga tacagtcaga attaagcaga gaatctcgtc gttttctcat aaaacaggga caaaattcgg tcgttgcaga actcaattta cgtggaatga acgatgagat cgccatctta agcggtacga ccaaaaacat cgaactggtg aaccaaatta tcaacgatta tggagcagat cccgatacat ggctgcctat atttcaccaa aggagagaaa atcaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat