SSGCID: Target Page

BaquA.18411.a

Type IV secretion system protein virB10 (VirB type IV secretion machinery)

CENTER ID:	BaquA.18411.a
ORGANISM:	Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaquA.18411.a.B2.GE36075	soluble domain		50	385

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaquA.18411.a.B2.PS01708	soluble domain		50	385

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283165.4.peg.1149
RefSeq:	YP_032628.1
UniProt:	Q6FYW1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKDEIDENNI NDRSTIKDGQ GKKLHSNTSK AVALLVLLGV CGYLAYSTLI TNKKQPVELP KEAIIKQTER FRPAQPKPVL LEPTEKNNLL LPKVELPTPK RNQTNADDSL LEAAQRAPVL AYASPQKSQA NAEKNNDTSP NQLERKPDET AQRFNHLLKP TNLEGIHAST LTNRNYIIAM GASIPCILET AISSDQQGFT SCIVSRDILS DNGRVVLLDK GTQIVGEYRS GLKKGQNRLF VLWNRAKTPS GVIITLASPA TDALGRSGVD GDVDNHWFER IGSALLVSIV RDATNYARNR LPKDQDKNSS DTISSGPNIA NIVVENYANI PPTLTKNQGE MVNVFVARDL DFSSVYKLKV IEDKKQIVNR SISRNFYKNS AVILK

NT Sequence

atgaaggatg aaatagatga aaacaacata aatgatcgca gtacaataaa agacggtcaa ggaaaaaaac tgcattccaa tacaagtaaa gcagttgctc ttctcgttct tttgggtgtt tgtggttatt tagcgtattc aacgcttatc acaaacaaaa aacagccggt tgaacttcca aaagaggcga ttattaagca aacagagcgt ttccgccctg cacagcccaa gcctgtactc ctcgagccaa ctgaaaaaaa taacctcctg ttgcccaagg ttgaattgcc aacgcccaaa agaaaccaaa caaatgctga tgattcactc ttagaagcag cacaacgtgc tcctgtctta gcctatgcca gcccacaaaa aagccaagca aatgcagaaa aaaataacga cacttcaccg aaccaactcg aaagaaaacc tgatgaaaca gcacaacgct ttaatcatct tctcaaaccg acaaaccttg aaggtattca tgcttcaaca cttactaatc gaaactacat catcgcaatg ggtgcttcca ttccatgtat tttagaaacg gcaatcagca gtgaccaaca gggatttacc agttgtatcg tttctagaga tatcttgtca gacaatggtc gcgttgtcct tcttgacaag ggcacacaaa tcgttggcga ataccgttcc ggattaaaaa aggggcaaaa tcgtcttttt gtgttatgga atagagccaa aacaccaagt ggtgttatta taacattagc ctcaccggca acggatgcct tagggcgttc tggtgttgat ggagatgttg ataatcattg gtttgagcgg attggatctg cgcttcttgt atcgattgtc agagatgcaa ccaattatgc gagaaaccgg ttgccaaaag atcaagataa gaacagttct gacacaatct cttcgggacc aaatattgca aatattgtcg tggaaaatta cgccaacatt cctcccacac taacaaaaaa ccaaggggaa atggtcaatg tttttgttgc ccgcgattta gatttttcca gtgtttataa attgaaagtg atcgaagaca aaaaacagat tgtcaatcga tccatttcaa gaaactttta taaaaattct gcggtgattt tgaaatga

Details for BaquA.18411.a.B2.GE36075

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	38kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	59

Validated AA Sequence

MGASIPCILE TAISSDQQGF TSCIVSRDIL SDNGRVVLLD KGTQIVGEYR SGLKKGQNRL FVLWNRAKTP SGVIITLASP ATDALGRSGV DGDVDNHWFE RIGSALLVSI VRDATNYARN RLPKDQDKNS SDTISSGPNI ANIVVENYAN IPPTLTKNQG EMVNVFVARD LDFSSVYKLK VIEDKKQIVN RSISRNFYKN SAVILK

Validated NT Sequence

tactcactta tttcaaaatc accgcagaat ttttataaaa gtttcttgaa atggatcgat tgacaatctg ttttttgtct tcgatcactt tcaatttata aacactggaa aaatctaaat cgcgggcaac aaaaacattg accatttccc cttggttttt tgttagtgtg ggaggaatgt tggcgtaatt ttccacgaca atatttgcaa tatttggtcc cgaagagatt gtgtcagaac tgttcttatc ttgatctttt ggcaaccggt ttctcgcata attggttgca tctctgacaa tcgatacaag aagcgcagat ccaatccgct caaaccaatg attatcaaca tctccatcaa caccagaacg ccctaaggca tccgttgccg gtgaggctaa tgttataata acaccacttg gtgttttggc tctattccat aacacaaaaa gacgattttg cccctttttt aatccggaac ggtattcgcc aacgatttgt gtgcccttgt caagaaggac aacgcgacca ttgtctgaca agatatctct agaaacgata caactggtaa atccctgttg gtcactgctg attgccgttt ctaaaataca tggaatggaa gcacccattg cgatgatgta gtttcgatta gtaagtgttg aagcatgaat accttcaagg tttgtcggtt tgagaagatg attaaagcgt tgtgctgttt catcaggttt tctttcgagt tggttcggtg aagtgtcgtt atttttttct gcatttgctt ggcttttttg tgggctggca taggctaaga caggagcacg ttgtgctgct tctaagagtg aatcatcagc atttgtttgg tttcttttgg gcgttggcaa ttcaaccttg ggcaacagga ggttattttt ttcagttggc tcgaggagta caggcttggg ctgtgcaggg cggaaacgct ctgtttgctt aataatcgcc tcttttggaa gttcaaccgg ctgttttttg t

Expected Protein Sequence

MAHHHHHHMI TNKKQPVELP KEAIIKQTER FRPAQPKPVL LEPTEKNNLL LPKVELPTPK RNQTNADDSL LEAAQRAPVL AYASPQKSQA NAEKNNDTSP NQLERKPDET AQRFNHLLKP TNLEGIHAST LTNRNYIIAM GASIPCILET AISSDQQGFT SCIVSRDILS DNGRVVLLDK GTQIVGEYRS GLKKGQNRLF VLWNRAKTPS GVIITLASPA TDALGRSGVD GDVDNHWFER IGSALLVSIV RDATNYARNR LPKDQDKNSS DTISSGPNIA NIVVENYANI PPTLTKNQGE MVNVFVARDL DFSSVYKLKV IEDKKQIVNR SISRNFYKNS AVILK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatca caaacaaaaa acagccggtt gaacttccaa aagaggcgat tattaagcaa acagagcgtt tccgccctgc acagcccaag cctgtactcc tcgagccaac tgaaaaaaat aacctcctgt tgcccaaggt tgaattgcca acgcccaaaa gaaaccaaac aaatgctgat gattcactct tagaagcagc acaacgtgct cctgtcttag cctatgccag cccacaaaaa agccaagcaa atgcagaaaa aaataacgac acttcaccga accaactcga aagaaaacct gatgaaacag cacaacgctt taatcatctt ctcaaaccga caaaccttga aggtattcat gcttcaacac ttactaatcg aaactacatc atcgcaatgg gtgcttccat tccatgtatt ttagaaacgg caatcagcag tgaccaacag ggatttacca gttgtatcgt ttctagagat atcttgtcag acaatggtcg cgttgtcctt cttgacaagg gcacacaaat cgttggcgaa taccgttccg gattaaaaaa ggggcaaaat cgtctttttg tgttatggaa tagagccaaa acaccaagtg gtgttattat aacattagcc tcaccggcaa cggatgcctt agggcgttct ggtgttgatg gagatgttga taatcattgg tttgagcgga ttggatctgc gcttcttgta tcgattgtca gagatgcaac caattatgcg agaaaccggt tgccaaaaga tcaagataag aacagttctg acacaatctc ttcgggacca aatattgcaa atattgtcgt ggaaaattac gccaacattc ctcccacact aacaaaaaac caaggggaaa tggtcaatgt ttttgttgcc cgcgatttag atttttccag tgtttataaa ttgaaagtga tcgaagacaa aaaacagatt gtcaatcgat ccatttcaag aaacttttat aaaaattctg cggtgatttt gaaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for BaquA.18411.a.B2.PS01708

PURIFICATION DATe:	1/2/2013
CONCENTRATION:	24.2mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	59

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCA CAAACAAAAA ACAGCCGGTT GAACTTCCAA AAGAGGCGAT TATTAAGCAA ACAGAGCGTT TCCGCCCTGC ACAGCCCAAG CCTGTACTCC TCGAGCCAAC TGAAAAAAAT AACCTCCTGT TGCCCAAGGT TGAATTGCCA ACGCCCAAAA GAAACCAAAC AAATGCTGAT GATTCACTCT TAGAAGCAGC ACAACGTGCT CCTGTCTTAG CCTATGCCAG CCCACAAAAA AGCCAAGCAA ATGCAGAAAA AAATAACGAC ACTTCACCGA ACCAACTCGA AAGAAAACCT GATGAAACAG CACAACGCTT TAATCATCTT CTCAAACCGA CAAACCTTGA AGGTATTCAT GCTTCAACAC TTACTAATCG AAACTACATC ATCGCAATGG GTGCTTCCAT TCCATGTATT TTAGAAACGG CAATCAGCAG TGACCAACAG GGATTTACCA GTTGTATCGT TTCTAGAGAT ATCTTGTCAG ACAATGGTCG CGTTGTCCTT CTTGACAAGG GCACACAAAT CGTTGGCGAA TACCGTTCCG GATTAAAAAA GGGGCAAAAT CGTCTTTTTG TGTTATGGAA TAGAGCCAAA ACACCAAGTG GTGTTATTAT AACATTAGCC TCACCGGCAA CGGATGCCTT AGGGCGTTCT GGTGTTGATG GAGATGTTGA TAATCATTGG TTTGAGCGGA TTGGATCTGC GCTTCTTGTA TCGATTGTCA GAGATGCAAC CAATTATGCG AGAAACCGGT TGCCAAAAGA TCAAGATAAG AACAGTTCTG ACACAATCTC TTCGGGACCA AATATTGCAA ATATTGTCGT GGAAAATTAC GCCAACATTC CTCCCACACT AACAAAAAAC CAAGGGGAAA TGGTCAATGT TTTTGTTGCC CGCGATTTAG ATTTTTCCAG TGTTTATAAA TTGAAAGTGA TCGAAGACAA AAAACAGATT GTCAATCGAT CCATTTCAAG AAACTTTTAT AAAAATTCTG CGGTGATTTT GAAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT