SSGCID: Target Page

BaquA.18408.a

TrwK protein (Trw type IV secretion machinery)

CENTER ID:	BaquA.18408.a
ORGANISM:	Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaquA.18408.a.B1.GE36074	full length		1	823

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283165.4.peg.1376
RefSeq:	YP_032785.1
UniProt:	Q6FYJ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTAVESIKRL ASETPVSLFI PYSHHVTDTI ISTKNAEYLS VWKIDGRSHQ SASEEDIFRW TKELNNTLRG IASANLSLWT HIVRRRVYEY PDSTFDKIFC YQLDEKYRQS FVGYNLMVND LYLTIVFQPV TDKVMSFFSQ HERETLDQKK MRQESCIKAL NDINRTLSQS FKCYGAELLG AYEKNGHAYS SALEFLAMLV NGEYQPMPIC HDRFADYMSI NRPFFSKWGA LGELRTTTGM RRFGMMEIKE YDTTTKPGQL NVLLESDFEF VLTQSFSVLS RHAAKDYLQR HQRNLIDARD VATSQIEQID EALNQLVSGH FVMGEHHCTL TIYGDTIQQV RDYMAKANAD MLDVGVLPKP VDLAIEAGYW AQLPGNWKWR PRPAPITSLN FLSFSSFHNF MSGKPTGNPW GPAVTILKTI SKTPLYFNFH ASKLEEDATN KRLLGNTAII GQSGSGKTVL LGFLLAQAQK FKPTTVVFDK DRGMEIAIRA MGGRYLTFKP GRPSSFNPFQ LPPTQDNLTF LKQFVKKLAE AGGEITHRDE EEINQAVISI MSSNIDRSLR RLSFLLQFLP NPRSSHYNAH PSVHARLAKW CQNGDYGWLF DNPQDALDLS THQIYGFDIT EFLDNPETRT PVIMYLFYRT EGMISGQRFM YIFDEFWKSL QDPYFEDLAK NKQKTIRKQN GIFVFATQEP SDALESNIAK TLIQQCATYI FLANPKASYE DYTEGFKLTD AEFELVKGLG EFSRQFLIKQ GDQSALAELN LGKFNVTTDG KTIERDFSDE LLVLSGTPDN AELVETLIAE VGDDPAVWLP VFLQHIKTER RVI

NT Sequence

atgacggctg ttgaaagcat aaaacggctt gcatcagaaa caccagtaag tttgtttata ccctattcac accacgttac agatacgatt atctctacca aaaatgcgga gtatttgtcg gtctggaaaa ttgatggacg ctcacatcaa agcgcttcag aagaggatat ttttcggtgg actaaagagc taaacaatac cctgcgtggt attgcatcgg ctaatttatc actgtggaca catatcgtac gtcgacgtgt ttatgaatat cctgattcaa catttgataa aatattttgc tatcagcttg atgaaaagta ccgacaaagt tttgttggtt ataatctaat ggtcaatgat ctgtatttga ccattgtgtt ccaaccagtc actgataagg ttatgtcgtt tttttcccag catgaacgtg aaacgcttga tcaaaaaaag atgcggcaag aatcatgtat taaagctctc aatgatatca accgtacctt aagtcaatcg tttaaatgtt atggtgccga acttcttggt gcatatgaaa aaaatggtca cgcttattct tctgcccttg aatttctcgc aatgcttgtc aatggtgaat accagcctat gccaatctgc catgaccgtt ttgctgacta tatgtctatc aatcggcctt tcttctcaaa atggggagca ctgggtgaac tgcgcacaac cactggaatg cgccgatttg gaatgatgga aattaaggaa tatgacacaa caaccaaacc gggccagtta aatgtcttac tggaaagtga tttcgaattt gtgttaacac agagcttttc tgtactctcg cgccatgcag ccaaggatta tttgcagcgg catcaacgga atcttatcga cgctcgcgat gtagcaacaa gtcaaattga gcaaattgac gaagcactta atcaacttgt aagtgggcat tttgtcatgg gtgagcacca ttgcacattg accatctacg gcgatacaat tcaacaagtt cgcgactata tggcaaaagc gaatgcggat atgctagacg ttggcgtgtt accaaaacct gtagatttgg ccatagaagc tggctattgg gcacaacttc ccggaaattg gaaatggcga ccacgtcccg caccaattac gtcattgaac tttttatcat tctcatcttt tcataacttt atgtcaggca aaccaacggg gaatccatgg gggccggcag ttacaattct taaaacaata agtaaaacac cgctttattt caattttcat gcctctaaac tcgaagaaga tgcgacaaat aagcggttac ttggtaatac tgcaatcatt ggacagtctg gttctggtaa aaccgtcctt cttggatttt tgttagcgca agcgcaaaaa ttcaaaccga caactgtcgt ttttgataaa gatcgtggca tggaaattgc cattcgtgct atggggggac ggtatctaac attcaaacct ggtaggccaa gtagttttaa tccgttccaa cttccaccga cacaagacaa cctaactttt cttaaacaat ttgttaaaaa attggcagaa gctggtggcg aaatcacaca ccgcgacgaa gaggaaatta atcaagctgt catatctatt atgagcagta acattgaccg atcgctccgt cgtttgtctt ttctgctgca attcttaccc aatccgcgtt caagtcatta taatgcacac ccatctgttc acgctcgctt ggcaaaatgg tgccaaaatg gtgattatgg gtggctcttt gataatcctc aggatgcact tgatctctca acgcatcaaa tttacggctt tgatattaca gaattcttag ataatccaga gactcgtact ccagtgataa tgtacttgtt ctatcgtaca gaaggaatga tcagtgggca acggtttatg tatatctttg acgagttttg gaaatcttta caggatccgt attttgaaga tttagcaaag aacaagcaaa aaactattcg taaacaaaac ggtatttttg tttttgctac gcaagagcct agtgatgcgc tggaaagcaa tattgctaag acgctcattc aacaatgtgc aacctacatt tttctagcca atcccaaagc gagttatgaa gattatactg aaggcttcaa actaacagat gctgaatttg aactggtcaa aggacttggt gaatttagtc gtcagttcct catcaagcaa ggtgatcagt ctgcgcttgc cgagcttaat ctcggcaaat ttaatgtcac aactgatgga aaaactatcg aacgtgactt tagtgacgaa ctcttagtat tatccggtac gcctgacaat gcagagttgg tcgaaacact tatcgctgaa gtcggagacg atccagcggt atggttgcca gtttttttac aacatataaa aactgagagg agagtaatat ga

Details for BaquA.18408.a.B1.GE36074

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	95kDa
OBSERVED MW:	80kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	98
PERCENT COVERAGE:	28

Validated AA Sequence

MAHHHHHHMT AVESIKRLAS ETPVSLFIPY SHHVTDTIIS TKNAEYLSVW KIDGRSHQSA SEEDIFRWTK ELNNTLRGIA SANLSLWTHI IRRRVYEYPD STFDKIFCYQ LDEKYRQSFV GYNLMVNDLY LTIVFQPVTD KVMSFFSQHE RETLDQKKMR QESCIKALND INRTLSQSFK CYGAELLGAY EKNGHAYSSA LEFLAMLVNG EYQPMPICHD RFADYMSINR PFFSKWGSTG

Validated NT Sequence

attttntnnn nnttaagaag gaganatacc atggctcacc accaccacca ccatatgacg gctgttgaaa gcataaaacg gcttgcatca gaaacaccag taagtttgtt tataccctat tcacaccacg ttacagatac gattatctct accaaaaatg cggagtattt gtcggtctgg aaaattgacg gacgctcaca tcaaagcgct tcagaagagg atatttttcg gtggactaaa gagctaaaca acaccctgcg tggtattgca tcggctaatt tatcactgtg gacacatatc atacgtcgac gtgtttatga atatcctgat tcaacatttg ataaaatatt ttgctatcag cttgatgaaa agtaccgaca aagttttgtt ggttataatc taatggtcaa tgatctgtat ttgaccattg tgttccaacc agtcactgat aaggttatgt cgtttttttc ccagcatgaa cgtgaaacgc ttgatcaaaa aaagatgcgg caagaatcat gtattaaagc tctcaatgat atcaaccgta ccttaagtca atcgtttaaa tgttatggtg ccgaacttct tggtgcatat gaaaaaaatg gtcacgctta ttcttctgcc cttgaatttc tcgcaatgct tgtcaatggt gaataccagc ctatgccaat ctgccatgac cgttttgctg actatatgtc tatcaatcgg cctttcttct caaaatgggg gagcactggg tgaactgcgc acaaccactg gaatgcgccg atttggaata atggaaatta aggaatatga cacgacnacc aaaccgggcc aattaaatgt cttactggaa agtgatttcn aatttgtgtt aacacagagc ttttctgtac tctcgcgcnn tgcagcnnng nattatttgc agcggcatca acgcnatcnt atcgacgctc gcgatgtagc annagccaaa ntgagcannt gacnannacn tannanntgn annggnattt gncatgggtg ancncnnngt nncnnnnacg nnannnnnnn nnnnnnnnnc nnnnngnaan nnnnnngntn nnanannnnn nnnntnnnnn nnnnnnnnnn nnnaannnng nnnnnnnnnn nnnnnnnnnn a

Expected Protein Sequence

MAHHHHHHMT AVESIKRLAS ETPVSLFIPY SHHVTDTIIS TKNAEYLSVW KIDGRSHQSA SEEDIFRWTK ELNNTLRGIA SANLSLWTHI VRRRVYEYPD STFDKIFCYQ LDEKYRQSFV GYNLMVNDLY LTIVFQPVTD KVMSFFSQHE RETLDQKKMR QESCIKALND INRTLSQSFK CYGAELLGAY EKNGHAYSSA LEFLAMLVNG EYQPMPICHD RFADYMSINR PFFSKWGALG ELRTTTGMRR FGMMEIKEYD TTTKPGQLNV LLESDFEFVL TQSFSVLSRH AAKDYLQRHQ RNLIDARDVA TSQIEQIDEA LNQLVSGHFV MGEHHCTLTI YGDTIQQVRD YMAKANADML DVGVLPKPVD LAIEAGYWAQ LPGNWKWRPR PAPITSLNFL SFSSFHNFMS GKPTGNPWGP AVTILKTISK TPLYFNFHAS KLEEDATNKR LLGNTAIIGQ SGSGKTVLLG FLLAQAQKFK PTTVVFDKDR GMEIAIRAMG GRYLTFKPGR PSSFNPFQLP PTQDNLTFLK QFVKKLAEAG GEITHRDEEE INQAVISIMS SNIDRSLRRL SFLLQFLPNP RSSHYNAHPS VHARLAKWCQ NGDYGWLFDN PQDALDLSTH QIYGFDITEF LDNPETRTPV IMYLFYRTEG MISGQRFMYI FDEFWKSLQD PYFEDLAKNK QKTIRKQNGI FVFATQEPSD ALESNIAKTL IQQCATYIFL ANPKASYEDY TEGFKLTDAE FELVKGLGEF SRQFLIKQGD QSALAELNLG KFNVTTDGKT IERDFSDELL VLSGTPDNAE LVETLIAEVG DDPAVWLPVF LQHIKTERRV I

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgg ctgttgaaag cataaaacgg cttgcatcag aaacaccagt aagtttgttt ataccctatt cacaccacgt tacagatacg attatctcta ccaaaaatgc ggagtatttg tcggtctgga aaattgatgg acgctcacat caaagcgctt cagaagagga tatttttcgg tggactaaag agctaaacaa taccctgcgt ggtattgcat cggctaattt atcactgtgg acacatatcg tacgtcgacg tgtttatgaa tatcctgatt caacatttga taaaatattt tgctatcagc ttgatgaaaa gtaccgacaa agttttgttg gttataatct aatggtcaat gatctgtatt tgaccattgt gttccaacca gtcactgata aggttatgtc gtttttttcc cagcatgaac gtgaaacgct tgatcaaaaa aagatgcggc aagaatcatg tattaaagct ctcaatgata tcaaccgtac cttaagtcaa tcgtttaaat gttatggtgc cgaacttctt ggtgcatatg aaaaaaatgg tcacgcttat tcttctgccc ttgaatttct cgcaatgctt gtcaatggtg aataccagcc tatgccaatc tgccatgacc gttttgctga ctatatgtct atcaatcggc ctttcttctc aaaatgggga gcactgggtg aactgcgcac aaccactgga atgcgccgat ttggaatgat ggaaattaag gaatatgaca caacaaccaa accgggccag ttaaatgtct tactggaaag tgatttcgaa tttgtgttaa cacagagctt ttctgtactc tcgcgccatg cagccaagga ttatttgcag cggcatcaac ggaatcttat cgacgctcgc gatgtagcaa caagtcaaat tgagcaaatt gacgaagcac ttaatcaact tgtaagtggg cattttgtca tgggtgagca ccattgcaca ttgaccatct acggcgatac aattcaacaa gttcgcgact atatggcaaa agcgaatgcg gatatgctag acgttggcgt gttaccaaaa cctgtagatt tggccataga agctggctat tgggcacaac ttcccggaaa ttggaaatgg cgaccacgtc ccgcaccaat tacgtcattg aactttttat cattctcatc ttttcataac tttatgtcag gcaaaccaac ggggaatcca tgggggccgg cagttacaat tcttaaaaca ataagtaaaa caccgcttta tttcaatttt catgcctcta aactcgaaga agatgcgaca aataagcggt tacttggtaa tactgcaatc attggacagt ctggttctgg taaaaccgtc cttcttggat ttttgttagc gcaagcgcaa aaattcaaac cgacaactgt cgtttttgat aaagatcgtg gcatggaaat tgccattcgt gctatggggg gacggtatct aacattcaaa cctggtaggc caagtagttt taatccgttc caacttccac cgacacaaga caacctaact tttcttaaac aatttgttaa aaaattggca gaagctggtg gcgaaatcac acaccgcgac gaagaggaaa ttaatcaagc tgtcatatct attatgagca gtaacattga ccgatcgctc cgtcgtttgt cttttctgct gcaattctta cccaatccgc gttcaagtca ttataatgca cacccatctg ttcacgctcg cttggcaaaa tggtgccaaa atggtgatta tgggtggctc tttgataatc ctcaggatgc acttgatctc tcaacgcatc aaatttacgg ctttgatatt acagaattct tagataatcc agagactcgt actccagtga taatgtactt gttctatcgt acagaaggaa tgatcagtgg gcaacggttt atgtatatct ttgacgagtt ttggaaatct ttacaggatc cgtattttga agatttagca aagaacaagc aaaaaactat tcgtaaacaa aacggtattt ttgtttttgc tacgcaagag cctagtgatg cgctggaaag caatattgct aagacgctca ttcaacaatg tgcaacctac atttttctag ccaatcccaa agcgagttat gaagattata ctgaaggctt caaactaaca gatgctgaat ttgaactggt caaaggactt ggtgaattta gtcgtcagtt cctcatcaag caaggtgatc agtctgcgct tgccgagctt aatctcggca aatttaatgt cacaactgat ggaaaaacta tcgaacgtga ctttagtgac gaactcttag tattatccgg tacgcctgac aatgcagagt tggtcgaaac acttatcgct gaagtcggag acgatccagc ggtatggttg ccagtttttt tacaacatat aaaaactgag aggagagtaa tatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat