SSGCID: Target Page

BaquA.18407.a

TrwE protein (Trw type IV secretion machinery)

CENTER ID:	BaquA.18407.a
ORGANISM:	Bartonella quintana Toulouse
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BaquA.18407.a.B2.GE36073	soluble domain		63	390

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|283165.4.peg.1385
RefSeq:	YP_032794.1
UniProt:	A0A0H3LV32

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFDNKKGDEI NTGAEQDYTE HKHIEGAYGS SELDSERRPI VPGARTLLIV GLIVIVALPI ALSWKALKMR NAIDVEEEKP QQTVEQIIPR YTPRITEEPK ILEEPEKPVQ AEELTQSIPP ALAQLLQTMP PHLIQDAEEL ARKRMLSSGL NNGSSSESSA ENSKKNTFDN GNSGVLFDKL QPVRLGQSHA AQLRNRDLLI TQGTQIDCTL ETKIVTSQPG MTTCHLTRDV YSTSGRVVLL DRGSKVVGFY QSGIQQGQTR VFVQWSRIET PSGVIVNLDS PGTGPLGESG IDGWIDTHFW QRFGGAIMMS IIGDLGGWVK NKIGQGNKES KEGKEKSLSQ NTQGPESVVN EVLHNSINIP PTLYKNQGER VSIFVARDLD FSDVYSLIIR

NT Sequence

atgttcgaca ataagaaagg agatgagatc aatacaggtg cagaacaaga ctacactgag cataaacata ttgaaggtgc gtatggtagc tccgaattag actcggaacg ccgtccaata gttcccggtg cacggacatt gttaatagta gggctcattg tcatagtagc gttgccgatt gctctgtctt ggaaggcttt aaaaatgcgc aatgccatag acgttgaaga agaaaagcct caacagacag tagagcaaat tatacctcgc tacacccctc gaatcacaga ggaaccgaaa atcttagaag aaccagaaaa accagtacag gcagaagaac taacgcagtc tattccacca gccttagcac aacttcttca aacaatgcca ccacatttga tccaggatgc tgaagaattg gcacgcaaac gtatgttaag ttctggtctt aacaatggta gcagtagcga aagctctgca gaaaattcaa aaaagaatac atttgacaat ggaaatagtg gtgtattatt cgataagctc cagcctgtac gattaggtca atcacacgcg gcccaactgc gtaaccgtga tctcttgatt acgcagggaa cgcaaataga ttgtacgctg gaaacaaaaa tcgtcacatc acaaccggga atgacaacat gtcatttaac acgtgatgtc tattctacga gcggtcgtgt tgttttgctc gatcgtggtt ccaaagtcgt cggtttctac caaagtggca tacaacaagg acaaacgcgc gttttcgtgc aatggtcgcg tattgaaacg ccgtctggtg ttattgtcaa tcttgactca cctggtactg gccctcttgg ggaatctggg atcgatggtt ggattgatac acatttctgg caaagatttg gcggtgcaat catgatgagt ataatcggtg atttgggagg atgggtgaaa aataaaattg gtcaaggaaa taaagaaagc aaagaaggca aggaaaaatc actatctcaa aatacacagg gtcctgaatc agtggttaac gaagttcttc acaattcaat caacattcca ccaacacttt acaaaaacca aggcgagcgg gtgagtattt ttgttgctcg cgatctggat ttcagcgatg tctacagcct cattatacgc taa

Details for BaquA.18407.a.B2.GE36073

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/23/2013
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	92

Validated AA Sequence

MAHHHHHHMS WKALKMRNAI DVEEEKPQQT VEQIIPRYTP RITEEPKILE EPEKPVQAEE LTQSIPPALA QLLQTMPPHL IQDAEELARK RMLSSGLNNG SSSESSAENS KKNTFDNGNS GVLFDKLQPV RLGQSHAAQL RNRDLLITQG TQIDCTLETK IVTSQPGMTT CHLTRDVYST SGRVVLLDRG SKVVGFYQSG IQQGQTRVFV QWSRIETPSG VIVNLDSPGT GPLGESGIDG WIDTHFWQRF GGAIMMSIIG DLGGWVKNKI GQGNKESKEX KEKSLSQNTQ GPESVVNEVL HNSINIXXXT LQ

Validated NT Sequence

atggctcacc accaccacca ccatatgtct tggaaggctt taaaaatgcg caatgccata gacgttgaag aagaaaagcc tcaacagaca gtagagcaaa ttatacctcg ctacacccct cgaatcacag aggaaccgaa aatcttagaa gaaccagaaa aaccagtaca ggcagaagaa ctaacgcagt ctattccacc agccttagca caacttcttc aaacaatgcc accacatttg atccaggatg ctgaagaatt ggcacgcaaa cgtatgttaa gttctggtct taacaatggt agcagtagcg aaagctctgc agaaaattca aaaaagaata catttgacaa tggaaatagt ggtgtattat tcgataagct ccagcctgta cgattaggtc aatcacacgc ggcccaactg cgtaaccgtg atctcttgat tacgcaggga acgcaaatag attgtacgct ggaaacaaaa atcgtcacat cacaaccggg aatgacaaca tgtcatttaa cacgtgatgt ctattctacg agcggtcgtg ttgttttgct cgatcgtggt tccaaagtcg tcggtttcta ccaaagtggc atacaacaag gacaaacgcg cgttttcgtg caatggtcgc gtattgaaac gccgtctggt gttattgtca atcttgactc acctggtact ggccctcttg gggaatctgg gatcgatggt tggattgata cacatttctg gcaaagattt ggcggtgcaa tcatgatgag tataatcggt gatttgggag gatgggtgaa aaataaaatt ggtcaaggaa ataaagaaag caaagaannn aaggaaaaat cactatctca aaatacacag ggtcctgaat cagtggttaa cgaagttctt cacaattcaa tcaacattnc nnnnnacact ttacaaaaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt ggaaggcttt aaaaatgcgc aatgccatag acgttgaaga agaaaagcct caacagacag tagagcaaat tatacctcgc tacacccctc gaatcacaga ggaaccgaaa atcttagaag aaccagaaaa accagtacag gcagaagaac taacgcagtc tattccacca gccttagcac aacttcttca aacaatgcca ccacatttga tccaggatgc tgaagaattg gcacgcaaac gtatgttaag ttctggtctt aacaatggta gcagtagcga aagctctgca gaaaattcaa aaaagaatac atttgacaat ggaaatagtg gtgtattatt cgataagctc cagcctgtac gattaggtca atcacacgcg gcccaactgc gtaaccgtga tctcttgatt acgcagggaa cgcaaataga ttgtacgctg gaaacaaaaa tcgtcacatc acaaccggga atgacaacat gtcatttaac acgtgatgtc tattctacga gcggtcgtgt tgttttgctc gatcgtggtt ccaaagtcgt cggtttctac caaagtggca tacaacaagg acaaacgcgc gttttcgtgc aatggtcgcg tattgaaacg ccgtctggtg ttattgtcaa tcttgactca cctggtactg gccctcttgg ggaatctggg atcgatggtt ggattgatac acatttctgg caaagatttg gcggtgcaat catgatgagt ataatcggtg atttgggagg atgggtgaaa aataaaattg gtcaaggaaa taaagaaagc aaagaaggca aggaaaaatc actatctcaa aatacacagg gtcctgaatc agtggttaac gaagttcttc acaattcaat caacattcca ccaacacttt acaaaaacca aggcgagcgg gtgagtattt ttgttgctcg cgatctggat ttcagcgatg tctacagcct cattatacgc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat